Genomes and Genes


Haibin Xia


Country: China


  1. Chen H, Li Y, Du C, Li Y, Zhao J, Zheng X, et al. Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression. J Biotechnol. 2018;288:23-29 pubmed publisher
    ..This new system could provide a potentially safer and more powerful tool for genome editing and therapeutic application. ..
  2. Shan L, Wang D, Mao Q, Xia H. Establishment of a DGK? Endogenous Promoter Luciferase Reporter HepG2 Cell Line for Studying the Transcriptional Regulation of DGK? Gene. Appl Biochem Biotechnol. 2018;: pubmed publisher
    ..The study provides a useful tool for high-throughput drug screening for the treatment of DGK?-involved diseases. ..
  3. Li Z, Zhao J, Muhammad N, Wang D, Mao Q, Xia H. Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene. Biotechnol Lett. 2018;40:1495-1506 pubmed publisher
  4. Li Y, Zhang W, Zhao J, Li S, Shan L, Zhu J, et al. Establishing a dual knock-out cell line by lentivirus based combined CRISPR/Cas9 and Loxp/Cre system. Cytotechnology. 2018;70:1595-1605 pubmed publisher
    ..This system should have great potential in applications for multiple genes knock-out in vitro. ..
  5. Zhang W, Chen H, Zheng X, Wang D, Ji H, Xia H, et al. Targeted genome correction by a single adenoviral vector simultaneously carrying an inducible zinc finger nuclease and a donor template. J Biotechnol. 2014;188:1-6 pubmed publisher
    ..The gene correction efficiency of the single Ad was significantly higher than that of the double Ad system. This novel vector will be a promising ZFN and donor delivery system for treatment of monogenic diseases. ..
  6. Xiao D, Zhang W, Li Y, Liu K, Zhao J, Sun X, et al. A novel luciferase knock-in reporter system for studying transcriptional regulation of the human Sox2 gene. J Biotechnol. 2016;219:110-6 pubmed publisher
    ..This novel system will be a useful tool to study the transcriptional regulation of Sox2, and has great potential in medical and industrial applications. ..
  7. Chen H, Wang D, Xia R, Mao Q, Xia H. A novel adenoviral vector carrying an all-in-one Tet-On system with an autoregulatory loop for tight, inducible transgene expression. BMC Biotechnol. 2015;15:4 pubmed publisher
    ..This system has great potential for a variety of applications, including gene therapy and the study of gene function. ..
  8. Li Y, Li Y, Zhao J, Wang D, Mao Q, Xia H. Production and characterization of domain-specific monoclonal antibodies against human ECM1. Protein Expr Purif. 2016;121:103-11 pubmed publisher
    ..In conclusion, the domain-specific anti-ECM1 MAbs produced in this study should provide a useful tool for investigating ECM1's biological functions, and cellular pathways in which it is involved. ..
  9. Li Y, Li Y, Ye M, Wang D, Zhao J, Sun X, et al. Biological function analysis of monoclonal antibodies against human granulins in vitro using U251 cells as a model. Protein Expr Purif. 2017;130:55-62 pubmed publisher

More Information


  1. request reprint
    Chen F, Zhang W, Zhao J, Yang P, Ma R, Xia H. [Construction of Rev-erb? gene knockout HEK293 cell line with CRISPR/Cas9 system]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1446-1452 pubmed
    ..Conclusion Using CRISPR/Cas9 technology, the HEK293 Rev-erb?-/- cell line has been successfully constructed, which would provide an effective tool for the study on the function of Rev-erb?. ..
  2. Chen F, Li Y, Zhao J, Mao Q, Xia H. Domain-Specific Monoclonal Antibodies Against Human Rev-erb?. Appl Biochem Biotechnol. 2017;182:978-989 pubmed publisher
    ..The MAbs produced in the study will provide a valuable tool for investigating the function of Rev-erb?. ..