Chen H, Li Y, Du C, Li Y, Zhao J, Zheng X, et al
. Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression. J Biotechnol. 2018;288:23-29 pubmed publisher
..This new system could provide a potentially safer and more powerful tool for genome editing and therapeutic application. ..
Shan L, Wang D, Mao Q, Xia H. Establishment of a DGK? Endogenous Promoter Luciferase Reporter HepG2 Cell Line for Studying the Transcriptional Regulation of DGK? Gene. Appl Biochem Biotechnol. 2018;: pubmed publisher
..The study provides a useful tool for high-throughput drug screening for the treatment of DGK?-involved diseases. ..
Li Z, Zhao J, Muhammad N, Wang D, Mao Q, Xia H. Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene. Biotechnol Lett. 2018;40:1495-1506 pubmed publisher
Li Y, Zhang W, Zhao J, Li S, Shan L, Zhu J, et al
. Establishing a dual knock-out cell line by lentivirus based combined CRISPR/Cas9 and Loxp/Cre system. Cytotechnology. 2018;70:1595-1605 pubmed publisher
..This system should have great potential in applications for multiple genes knock-out in vitro. ..
Zhang W, Chen H, Zheng X, Wang D, Ji H, Xia H, et al
. Targeted genome correction by a single adenoviral vector simultaneously carrying an inducible zinc finger nuclease and a donor template. J Biotechnol. 2014;188:1-6 pubmed publisher
..The gene correction efficiency of the single Ad was significantly higher than that of the double Ad system. This novel vector will be a promising ZFN and donor delivery system for treatment of monogenic diseases. ..
Xiao D, Zhang W, Li Y, Liu K, Zhao J, Sun X, et al
. A novel luciferase knock-in reporter system for studying transcriptional regulation of the human Sox2 gene. J Biotechnol. 2016;219:110-6 pubmed publisher
..This novel system will be a useful tool to study the transcriptional regulation of Sox2, and has great potential in medical and industrial applications. ..
Chen H, Wang D, Xia R, Mao Q, Xia H. A novel adenoviral vector carrying an all-in-one Tet-On system with an autoregulatory loop for tight, inducible transgene expression. BMC Biotechnol. 2015;15:4 pubmed publisher
..This system has great potential for a variety of applications, including gene therapy and the study of gene function. ..
Li Y, Li Y, Zhao J, Wang D, Mao Q, Xia H. Production and characterization of domain-specific monoclonal antibodies against human ECM1. Protein Expr Purif. 2016;121:103-11 pubmed publisher
..In conclusion, the domain-specific anti-ECM1 MAbs produced in this study should provide a useful tool for investigating ECM1's biological functions, and cellular pathways in which it is involved. ..
Li Y, Li Y, Ye M, Wang D, Zhao J, Sun X, et al
. Biological function analysis of monoclonal antibodies against human granulins in vitro using U251 cells as a model. Protein Expr Purif. 2017;130:55-62 pubmed publisher