Affiliation: Wuhan University
Tao W, Yurkovich M, Wen S, Lebe K, Samborskyy M, Yang A, et al
. A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. Chem Sci. 2016;7:376-385 pubmed publisher
..These insights have allowed us to propose a mechanism for sulfur insertion, and have opened the way to engineering of the biosynthesis of TLM and other thiotetronates to produce novel analogues. ..
Li S, Guo J, Reva A, Huang F, Xiong B, Liu Y, et al
. Methyltransferases of gentamicin biosynthesis. Proc Natl Acad Sci U S A. 2018;115:1340-1345 pubmed publisher
..These findings fully account for the methylation pattern in gentamicins and open the way to production of individual gentamicins by fermentation, as starting materials for semisynthesis. ..
Huang C, Huang F, Moison E, Guo J, Jian X, Duan X, et al
. Delineating the biosynthesis of gentamicin x2, the common precursor of the gentamicin C antibiotic complex. Chem Biol. 2015;22:251-61 pubmed publisher
..Finally, C-methylation at C-4â€³ to form gentamicin X2 is catalyzed by the radical SAM-dependent and cobalamin-dependent enzyme GenD1. ..
Zeng H, Wen S, Xu W, He Z, Zhai G, Liu Y, et al
. Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system. Appl Microbiol Biotechnol. 2015;99:10575-85 pubmed publisher
..We foresee the system becoming a routine laboratory technique for genome editing to exploit the great biosynthetic potential of Streptomyces and perhaps for other medically and economically important actinomycetes. ..
Liu Y, Tao W, Wen S, Li Z, Yang A, Deng Z, et al
. In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing. MBio. 2015;6:e01714-15 pubmed publisher
..This in vitro CRISPR editing (ICE) system promises a step forward in our ability to engineer biosynthetic pathways. ..