Bohai Wen

Summary

Country: China

Publications

  1. pmc Microarray of surface-exposed proteins of rickettsia heilongjiangensis for serodiagnosis of Far-eastern spotted fever
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai, Beijing, China
    BMC Infect Dis 14:332. 2014
  2. pmc Simultaneous detection of Anaplasma marginale and a new Ehrlichia species closely related to Ehrlichia chaffeensis by sequence analyses of 16S ribosomal DNA in Boophilus microplus ticks from Tibet
    Bohai Wen
    Department of Microbiology, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    J Clin Microbiol 40:3286-90. 2002
  3. ncbi Ehrlichiae and ehrlichial diseases in china
    Bohai Wen
    Department of Microbiology, Beijing Institute of Microbiology and Epidemiology, PR China
    Ann N Y Acad Sci 990:45-53. 2003
  4. ncbi Recombinant protein YbgF induces protective immunity against Rickettsia heilongjiangensis infection in C3H/HeN mice
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai, Beijing 100071, China
    Vaccine 31:5643-50. 2013
  5. pmc Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 8:e70440. 2013
  6. doi Protein array of Coxiella burnetii probed with Q fever sera
    Xile Wang
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    Sci China Life Sci 56:453-9. 2013
  7. doi Mice immunized with bone marrow-derived dendritic cells stimulated with recombinant Coxiella burnetii Com1 and Mip demonstrate enhanced bacterial clearance in association with a Th1 immune response
    Xiaolu Xiong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai District, Beijing 100071, China
    Vaccine 30:6809-15. 2012
  8. ncbi Analysis of immunoprotectivity of the recombinant OmpA of Rickettsia heilongjiangensis
    Yanmei Jiao
    Beijing Institute of Microbiology and Epidemiology and State Key Laboratory of Pathogen and Biosecurity, Fengtai, Beijing 100071, China
    Ann N Y Acad Sci 1063:261-5. 2005
  9. ncbi Balb/c mouse model and real-time quantitative polymerase chain reaction for evaluation of the immunoprotectivity against Q fever
    Jingbo Zhang
    Beijing Institute of Microbiology and Epidemiology, Fengtai, Beijing 100071, China
    Ann N Y Acad Sci 1063:171-5. 2005
  10. pmc Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen
    Changsong Duan
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Da Jie St, Fengtai, Beijing 100071, China
    J Bacteriol 193:5564-5. 2011

Collaborators

Detail Information

Publications21

  1. pmc Microarray of surface-exposed proteins of rickettsia heilongjiangensis for serodiagnosis of Far-eastern spotted fever
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai, Beijing, China
    BMC Infect Dis 14:332. 2014
    ..The laboratory diagnosis of FESF in hospitals is mainly based on serological methods. However, these methods need to cultivate rickettsial cells as diagnostic antigens, which is both burdensome and dangerous...
  2. pmc Simultaneous detection of Anaplasma marginale and a new Ehrlichia species closely related to Ehrlichia chaffeensis by sequence analyses of 16S ribosomal DNA in Boophilus microplus ticks from Tibet
    Bohai Wen
    Department of Microbiology, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    J Clin Microbiol 40:3286-90. 2002
    ..microplus ticks is a new species of the genus Ehrlichia and is most closely related to E. chaffeensis...
  3. ncbi Ehrlichiae and ehrlichial diseases in china
    Bohai Wen
    Department of Microbiology, Beijing Institute of Microbiology and Epidemiology, PR China
    Ann N Y Acad Sci 990:45-53. 2003
    ..These results reveal that a variety of tick-borne ehrlichial agents and diseases exist in China, and the ehrlichial agents and their tick-vectors are same as or different from that in other countries at species or strain levels...
  4. ncbi Recombinant protein YbgF induces protective immunity against Rickettsia heilongjiangensis infection in C3H/HeN mice
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai, Beijing 100071, China
    Vaccine 31:5643-50. 2013
    ..Surface proteins YbgF and PrsA are major seroreactive antigens of Rickettsia heilongjiangensis, the etiological agent of Far-Eastern spotted fever. This study investigated their potential immunogenicity for protective immunity...
  5. pmc Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis
    Yong Qi
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 8:e70440. 2013
    ..They are also potential molecular candidates for the development of diagnostic reagents and vaccines against rickettsiosis...
  6. doi Protein array of Coxiella burnetii probed with Q fever sera
    Xile Wang
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    Sci China Life Sci 56:453-9. 2013
    ..These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever...
  7. doi Mice immunized with bone marrow-derived dendritic cells stimulated with recombinant Coxiella burnetii Com1 and Mip demonstrate enhanced bacterial clearance in association with a Th1 immune response
    Xiaolu Xiong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai District, Beijing 100071, China
    Vaccine 30:6809-15. 2012
    ..Therefore, Com1 and Mip are key antigens to induce a protective immune response against C. burnetii infection...
  8. ncbi Analysis of immunoprotectivity of the recombinant OmpA of Rickettsia heilongjiangensis
    Yanmei Jiao
    Beijing Institute of Microbiology and Epidemiology and State Key Laboratory of Pathogen and Biosecurity, Fengtai, Beijing 100071, China
    Ann N Y Acad Sci 1063:261-5. 2005
    ..These results suggest that the truncated OmpA of R. heilongjiangii is immunogenic for effectively inducing humoral and cell-mediated immune responses against homologous and heterologous species in the spotted fever group...
  9. ncbi Balb/c mouse model and real-time quantitative polymerase chain reaction for evaluation of the immunoprotectivity against Q fever
    Jingbo Zhang
    Beijing Institute of Microbiology and Epidemiology, Fengtai, Beijing 100071, China
    Ann N Y Acad Sci 1063:171-5. 2005
    ..The results suggest that the combination of Balb/c model and the real-time quantitative PCR assay is a reliable and sensitive way to evaluate the efficiency of vaccines against Q fever...
  10. pmc Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen
    Changsong Duan
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Da Jie St, Fengtai, Beijing 100071, China
    J Bacteriol 193:5564-5. 2011
    ..Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054)...
  11. ncbi Genomic and comparative genomic analyses of Rickettsia heilongjiangensis provide insight into its evolution and pathogenesis
    Changsong Duan
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China Electronic address
    Infect Genet Evol 26:274-82. 2014
    ..heilongjiangensis and 42 other rickettsiae strains share 693 core genes with a pan-genome size of 4837 genes. The pan-genome-based phylogeny showed that R. heilongjiangensis was closely related to R. japonica. ..
  12. pmc Identification of novel surface-exposed proteins of Rickettsia rickettsii by affinity purification and proteomics
    Wenping Gong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 9:e100253. 2014
    ..rickettsii. Altogether, the 5 novel SEPs identified herein might be involved in the interaction of R. rickettsii with vascular endothelial cells, and all of them except TolC were protective antigens. ..
  13. pmc Potential serodiagnostic markers for Q fever identified in Coxiella burnetii by immunoproteomic and protein microarray approaches
    Xiaolu Xiong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Dong dia jie, Beijing 100071, China
    BMC Microbiol 12:35. 2012
    ..Coxiella burnetii is the etiological agent of Q fever. The clinical diagnosis of Q fever is mainly based on several serological tests. These tests all need Coxiella organisms which are difficult and hazardous to culture and purify...
  14. ncbi Surface protein Adr2 of Rickettsia rickettsii induced protective immunity against Rocky Mountain spotted fever in C3H/HeN mice
    Wenping Gong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Dia Jie street, Fengtai, Beijing 100071, China
    Vaccine 32:2027-33. 2014
    ..Adr2 was a surface-exposed adhesion protein of R. rickettsii and its immunoprotection against RMSF was investigated in mice...
  15. pmc Exploratory study on Th1 epitope-induced protective immunity against Coxiella burnetii infection
    Xiaolu Xiong
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 9:e87206. 2014
    ..burnetii infection. These observations could contribute to the rational design of molecular vaccines for Q fever. ..
  16. pmc Efficient activation of T cells by human monocyte-derived dendritic cells (HMDCs) pulsed with Coxiella burnetii outer membrane protein Com1 but not by HspB-pulsed HMDCs
    Ying Wang
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    BMC Immunol 12:52. 2011
    ..It is not clear whether Com1 and HspB have the ability to mount immune responses against C. burnetii infection...
  17. ncbi Protective immunity against Q fever induced with a recombinant P1 antigen fused with HspB of Coxiella burnetii
    Qingfeng Li
    Beijing Institute of Microbiology and Epidemiology, Fengtai, Beijing 100071, China
    Ann N Y Acad Sci 1063:130-42. 2005
    ..The data from this study indicate that fusion antigen P1-HspB is a good immunogen for eliciting immunoresponses against C. burnetii, and it may be a more suitable candidate for preparing subunit vaccine against Q fever...
  18. pmc Exploratory study on pathogenesis of far-eastern spotted fever
    Changsong Duan
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    Am J Trop Med Hyg 85:504-9. 2011
    ..i. Our results reveal that R. heilongjiangensis may cause an infection in BALB/c mice and the pathological lesions in the infected mice are associated with host inflammatory response induced by R. heilongjiangensis...
  19. pmc Coxiella burnetii antigen-stimulated dendritic cells mediated protection against Coxiella burnetii in BALB/c mice
    Yan Wei
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    J Infect Dis 203:283-91. 2011
    ..Our results suggest that PIIAg is far superior to PIAg in activating BMDCs to confer protection against C. burnetii in vivo, whereas Com1 and SecB are protective antigens because Com1- or SecB-pulsed BMDCs confer partial protection...
  20. ncbi A spiroplasma associated with tremor disease in the Chinese mitten crab (Eriocheir sinensis)
    Wen Wang
    Key Laboratory for Bioresources Technology of Jiangsu Province, College of Biological Sciences, Nanjing Normal University, Nanjing 210097, PR China
    Microbiology 150:3035-40. 2004
    ..This is believed to be the first time a spiroplasma has been found in a crustacean. These findings are not only significant for studies on pathogenic spiroplasmas, but also have implications for studies of freshwater ecology...
  21. ncbi Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis
    Xinzhong Wu
    College of Animal Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, China
    Dis Aquat Organ 67:149-54. 2005
    ..The present results advance our knowledge of RLO protein antigens, and several proteins have been identified that could potentially be useful for diagnostic assays or for production of experimental immunostimulants...