Jun C, Huan H, Wei F, Duozhi S, Chen L, Yang S, et al
. Detection of pathogens from resected heart valves of patients with infective endocarditis by next-generation sequencing. Int J Infect Dis. 2019;: pubmed publisher
..The NGS technology should be considered an essential supplement to culture-based methods, particularly for unculturable or difficult-to-culture microorganisms. ..
Cheng J, Hu H, Kang Y, Chen W, Fang W, Wang K, et al
. Identification of pathogens in culture-negative infective endocarditis cases by metagenomic analysis. Ann Clin Microbiol Antimicrob. 2018;17:43 pubmed publisher
..However, more efforts should be made to optimize protocols for sample processing, sequencing and bioinformatics analysis. ..