Kwok Yung Yuen

Summary

Affiliation: University of Hong Kong
Country: China

Publications

  1. pmc Genetic characterization of EV71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype D and recent emergence of a distinct lineage of subgenotype C2 in Hong Kong
    Cyril C Y Yip
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    Virol J 10:222. 2013
  2. pmc Comparative immunological evaluation of recombinant Salmonella Typhimurium strains expressing model antigens as live oral vaccines
    Song Yue Zheng
    Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong
    BMC Immunol 13:54. 2012
  3. pmc Transmission of methicillin-resistant Staphylococcus aureus in the long term care facilities in Hong Kong
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    BMC Infect Dis 13:205. 2013
  4. pmc Epidemiology of Klebsiella oxytoca-associated diarrhea detected by Simmons citrate agar supplemented with inositol, tryptophan, and bile salts
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Clin Microbiol 50:1571-9. 2012
  5. pmc Two years after pandemic influenza A/2009/H1N1: what have we learned?
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    Clin Microbiol Rev 25:223-63. 2012
  6. pmc Complete genome sequence of a novel picornavirus, canine picornavirus, discovered in dogs
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    J Virol 86:3402-3. 2012
  7. pmc Clostridium difficile isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong
    V C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    Eur J Clin Microbiol Infect Dis 30:1371-81. 2011
  8. pmc Transport genes and chemotaxis in Laribacter hongkongensis: a genome-wide analysis
    Susanna Kp Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    Cell Biosci 1:28. 2011
  9. pmc Virulence determinants, drug resistance and mobile genetic elements of Laribacter hongkongensis: a genome-wide analysis
    Susanna Kp Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    Cell Biosci 1:17. 2011
  10. pmc General metabolism of Laribacter hongkongensis: a genome-wide analysis
    Shirly O Curreem
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Cell Biosci 1:16. 2011

Collaborators

Detail Information

Publications243 found, 100 shown here

  1. pmc Genetic characterization of EV71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype D and recent emergence of a distinct lineage of subgenotype C2 in Hong Kong
    Cyril C Y Yip
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    Virol J 10:222. 2013
    ..The objective of this study was to investigate the genetic evolution of these EV71 isolates in Hong Kong over a 7-year period...
  2. pmc Comparative immunological evaluation of recombinant Salmonella Typhimurium strains expressing model antigens as live oral vaccines
    Song Yue Zheng
    Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong
    BMC Immunol 13:54. 2012
    ..Such information would provide invaluable guidance for the future rational design of live recombinant Salmonella oral vaccines...
  3. pmc Transmission of methicillin-resistant Staphylococcus aureus in the long term care facilities in Hong Kong
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    BMC Infect Dis 13:205. 2013
    ..The relative contribution of long term care facilities (LTCFs) and hospitals in the transmission of methicillin-resistant Staphylococcus aureus (MRSA) is unknown...
  4. pmc Epidemiology of Klebsiella oxytoca-associated diarrhea detected by Simmons citrate agar supplemented with inositol, tryptophan, and bile salts
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Clin Microbiol 50:1571-9. 2012
    ..oxytoca developed antibiotic-associated hemorrhagic colitis, suggesting that K. oxytoca can cause a mild disease manifesting as uncomplicated antibiotic-associated diarrhea with winter seasonality...
  5. pmc Two years after pandemic influenza A/2009/H1N1: what have we learned?
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    Clin Microbiol Rev 25:223-63. 2012
    ..These findings could prove useful for future randomized controlled clinical trials and the epidemiological control of future pandemics...
  6. pmc Complete genome sequence of a novel picornavirus, canine picornavirus, discovered in dogs
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    J Virol 86:3402-3. 2012
    ....
  7. pmc Clostridium difficile isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong
    V C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    Eur J Clin Microbiol Infect Dis 30:1371-81. 2011
    ..This finding should alert both the physician and the infection control team to the establishment of and possible outbreaks by ribotype 002 in our hospitals, as in the case of ribotype 027...
  8. pmc Transport genes and chemotaxis in Laribacter hongkongensis: a genome-wide analysis
    Susanna Kp Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    Cell Biosci 1:28. 2011
    ..abstract:..
  9. pmc Virulence determinants, drug resistance and mobile genetic elements of Laribacter hongkongensis: a genome-wide analysis
    Susanna Kp Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    Cell Biosci 1:17. 2011
    ..abstract:..
  10. pmc General metabolism of Laribacter hongkongensis: a genome-wide analysis
    Shirly O Curreem
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Cell Biosci 1:16. 2011
    ..abstract:..
  11. pmc Introduction of an electronic monitoring system for monitoring compliance with Moments 1 and 4 of the WHO "My 5 Moments for Hand Hygiene" methodology
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    BMC Infect Dis 11:151. 2011
    ..Unlike previous electronic monitoring systems, MedSense operates in open cubicles with multiple beds and does not disrupt existing workflows...
  12. pmc Sequential introduction of single room isolation and hand hygiene campaign in the control of methicillin-resistant Staphylococcus aureus in intensive care unit
    Vincent C C Cheng
    Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China
    BMC Infect Dis 10:263. 2010
    ..After renovation of the adult intensive care unit (ICU) with installation of ten single rooms, an enhanced infection control program was conducted to control the spread of methicillin-resistant Staphylococcus aureus (MRSA) in our hospital...
  13. pmc A model to control the epidemic of H5N1 influenza at the source
    Y Guan
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    BMC Infect Dis 7:132. 2007
    ..Systematic surveillance in southern China showed that ducks and geese are the primary reservoirs which transmit the virus to chickens, minor poultry and even migratory birds...
  14. pmc The unmasking of Pneumocystis jiroveci pneumonia during reversal of immunosuppression: case reports and literature review
    Alan K L Wu
    Division of Infectious Diseases, Centre of Infection, Queen Mary Hospital, The University of Hong Kong Hong Kong Special Administrative Region, China
    BMC Infect Dis 4:57. 2004
    ....
  15. pmc Differential selection and mutation between dsDNA and ssDNA phages shape the evolution of their genomic AT percentage
    Xuhua Xia
    Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada
    BMC Genet 6:20. 2005
    ....
  16. pmc Coronavirus genomics and bioinformatics analysis
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong China E Mail
    Viruses 2:1804-20. 2010
    ....
  17. pmc Draft genome sequence of Penicillium marneffei strain PM1
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Eukaryot Cell 10:1740-1. 2011
    ..We report the 28,887,485-bp draft genome sequence of P. marneffei, which contains its complete mitochondrial genome, sexual cycle genes, a high diversity of Mp1p homologues, and polyketide synthase genes...
  18. pmc Complete genome sequence of a novel paramyxovirus, Tailam virus, discovered in Sikkim rats
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong
    J Virol 85:13473-4. 2011
    ..1 to 94.4% and 23.8 to 80.1% amino acid identities to those of Beilong virus and J virus, respectively...
  19. ncbi request reprint The SARS attack on Hong Kong
    K Y Yuen
    Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong
    Hong Kong Med J 9:302-3. 2003
  20. ncbi request reprint Exploring the Penicillium marneffei genome
    Kwok Yung Yuen
    HKU Pasteur Research Centre, 8 Sassoon Road, Hong Kong
    Arch Microbiol 179:339-53. 2003
    ..Furthermore, the presence of genes encoding pheromone homologues and ankyrin repeat-containing proteins of other fungi and algae strongly suggests the presence of a sexual stage that presumably exists in the environment...
  21. pmc Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings
    C M Chu
    Department of Microbiology and Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong
    Thorax 59:252-6. 2004
    ....
  22. pmc Complete genome sequence of a coxsackievirus A22 strain in Hong Kong reveals a natural intratypic recombination event
    Cyril C Y Yip
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 85:12098-9. 2011
    ..We report the first complete genome sequence of CVA22 with natural intratypic recombination between CVA22 prototype strain Chulman and CVA22 strain ban99-10427, identified in the stool of a patient in Hong Kong...
  23. ncbi request reprint SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital, Hong Kong
    Vaccine 23:4959-68. 2005
    ..The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems...
  24. ncbi request reprint Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia
    Patrick C Y Woo
    Division of Infectious Disease, Department of Microbiology, The University of Hong Kong, Hong Kong, China
    J Infect Dis 192:1898-907. 2005
    ..Recently, we described the discovery of a novel group 2 coronavirus, coronavirus HKU1 (CoV-HKU1), from a patient with pneumonia. However, the clinical and molecular epidemiological features of CoV-HKU1-associated pneumonia are unknown...
  25. pmc Coronavirus HKU1 and other coronavirus infections in Hong Kong
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 44:2063-71. 2006
    ..Similar studies in other countries are required to better determine the epidemiology and genetic diversity of CoV-HKU1...
  26. ncbi request reprint Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
    Patrick C Y Woo
    Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    Lancet 363:841-5. 2004
    ....
  27. ncbi request reprint Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China
    J Infect Dis 196:986-93. 2007
    ..We conducted the first systematic prospective clinical and molecular study using nasopharyngeal aspirates (NPAs) and fecal samples...
  28. pmc Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia
    Patrick C Y Woo
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 42:2306-9. 2004
    ..The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV...
  29. ncbi request reprint Characterization of a novel cryptic plasmid, pHLHK26, in Laribacter hongkongensis
    Patrick C Y Woo
    Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong
    New Microbiol 30:139-47. 2007
    ....
  30. ncbi request reprint A novel approach for screening immunogenic proteins in Penicillium marneffei using the DeltaAFMP1DeltaAFMP2 deletion mutant of Aspergillus fumigatus
    Patrick C Y Woo
    Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong
    FEMS Microbiol Lett 262:138-47. 2006
    ..marneffei infection. A similar approach could be applied to immunogenic protein screening in other microorganisms for serological diagnosis, epidemiological studies and the study of vaccines...
  31. pmc Molecular epidemiology of human coronavirus OC43 reveals evolution of different genotypes over time and recent emergence of a novel genotype due to natural recombination
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 85:11325-37. 2011
    ....
  32. pmc Initial viral load and the outcomes of SARS
    Chung Ming Chu
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, China
    CMAJ 171:1349-52. 2004
    ..Preliminary data suggest that a high viral load of the SARS coronavirus is associated with adverse outcomes in the intensive care unit, but the relation of viral load to survival is unclear...
  33. ncbi request reprint Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish: a multicentre case-control study
    Patrick C Y Woo
    Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam, Hong Kong, People s Republic of China
    Lancet 363:1941-7. 2004
    ..We undertook a multicentre case-control study to investigate the association of L hongkongensis with gastroenteritis...
  34. pmc Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Antimicrob Agents Chemother 49:1957-64. 2005
    ..The beta-lactamase characterized from strain HLHK5 was named LHK-5 (gene, bla(LHK-5)) and represents the first example of AmpC beta-lactamase in the beta subdivision of proteobacteria...
  35. ncbi request reprint Eggerthella hongkongensis sp. nov. and eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    Diagn Microbiol Infect Dis 49:255-63. 2004
    ..The present study suggests that Eggerthella bacteremia is much more common than expected and is associated with significant morbidity and mortality. Moreover, the 2 novel species account for half of the cases of Eggerthella bacteremia...
  36. ncbi request reprint Streptococcus sinensis may react with Lancefield group F antiserum
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Med Microbiol 53:1083-8. 2004
    ..3 % S. anginosus, respectively. The present data revealed that a proportion of Lancefield group F streptococci could be S. sinensis. Lancefield group F streptococci should not be automatically reported as 'S. milleri'...
  37. pmc Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam, Hong Kong
    J Clin Microbiol 43:3054-8. 2005
    ..2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia...
  38. ncbi request reprint Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov
    Patrick C Y Woo
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China
    Diagn Microbiol Infect Dis 50:81-7. 2004
    ..9% differences between the 16S rRNA gene sequence of the bacterium and those of S. hydroxybenzoicus and S. saalensis respectively. A new species, Sedimentibacter hongkongenesis sp. nov., is proposed, for which HKU2(T) is the type strain...
  39. ncbi request reprint Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Hong Kong
    Microbiol Immunol 49:31-9. 2005
    ..hippei and P. vibrioides. The G+C content of the bacterium (mean +/- SD) was 46.8 +/- 3.2%. For these reasons, a new genus and species, Anaerospora hongkongensis gen. nov. sp. nov., is proposed, for which HKU15T is the type strain...
  40. pmc Detection of antibodies specific to an antigenic cell wall galactomannoprotein for serodiagnosis of Aspergillus fumigatus aspergillosis
    Che Man Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 40:2041-5. 2002
    ..Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis...
  41. pmc Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Clin Diagn Lab Immunol 11:665-8. 2004
    ..Further studies should be performed to see whether SARS-CoV nucleocapsid protein antibody positivity has any prognostic significance...
  42. pmc Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, Hong Kong
    J Clin Microbiol 41:4839-41. 2003
    ..In addition, with CMA, Arcobacter butzleri was unexpectedly isolated from the stools of six patients...
  43. pmc Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-
    Lanying Du
    Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    J Immunol 180:948-56. 2008
    ..n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile...
  44. doi request reprint Identification and complete genome analysis of three novel paramyxoviruses, Tuhoko virus 1, 2 and 3, in fruit bats from China
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    Virology 404:106-16. 2010
    ..Analysis of phosphoprotein gene mRNA products confirmed mRNA editing. Antibodies to the viruses were detected in 48-60% of Leschenault's rousettes...
  45. pmc Genetic characterization of Betacoronavirus lineage C viruses in bats reveals marked sequence divergence in the spike protein of pipistrellus bat coronavirus HKU5 in Japanese pipistrelle: implications for the origin of the novel Middle East respiratory sy
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China
    J Virol 87:8638-50. 2013
    ..Intensive surveillance for lineage C betaCoVs in Pipistrellus and related bats with diverse habitats and other animals in the Middle East may fill the evolutionary gap. ..
  46. ncbi request reprint Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Diagn Microbiol Infect Dis 54:177-81. 2006
    ..iniae infection. The Asian population and handlers of fresh fish should be informed of the risk of acquiring S. iniae infection...
  47. ncbi request reprint Recombinant adeno-associated virus expressing the receptor-binding domain of severe acute respiratory syndrome coronavirus S protein elicits neutralizing antibodies: Implication for developing SARS vaccines
    Lanying Du
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Virology 353:6-16. 2006
    ..5 months. These results suggested that RBD-rAAV is a promising SARS candidate vaccine...
  48. ncbi request reprint Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Syst Appl Microbiol 26:518-22. 2003
    ..nov., is proposed, for which HKU8(T) is the type strain. Further studies should be performed to ascertain the potential of this bacterium to become an important cause of actinomycosis...
  49. pmc Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavi
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong, and Guangzhou Center for Disease Control and Prevention, Guangzhou, China
    J Virol 86:3995-4008. 2012
    ....
  50. pmc Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 81:1574-85. 2007
    ..Further molecular epidemiological studies on coronaviruses in the bats of other countries, as well as in other animals, and complete genome sequencing will shed more light on coronavirus diversity and their evolutionary histories...
  51. doi request reprint Delayed induction of proinflammatory cytokines and suppression of innate antiviral response by the novel Middle East respiratory syndrome coronavirus: implications for pathogenesis and treatment
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, P R China
    J Gen Virol 94:2679-90. 2013
    ..Our results provide insights into the pathogenesis and treatment of MERS-CoV infections. ..
  52. doi request reprint Identification and characterization of a novel paramyxovirus, porcine parainfluenza virus 1, from deceased pigs
    Susanna K P Lau
    Department of Microbiology, University of Hong Kong, Hong Kong, PR China
    J Gen Virol 94:2184-90. 2013
    ..The presence of PPIV-1 in mainly respiratory samples suggests a possible association with respiratory disease, similar to HPIV-1 and SeV. ..
  53. pmc Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Research Centre of Infection and Immunology, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong, China
    J Virol 84:11385-94. 2010
    ..Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault's rousette...
  54. pmc Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 41:3973-7. 2003
    ..Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy...
  55. pmc Detection of cell wall galactomannoprotein Afmp1p in culture supernatants of Aspergillus fumigatus and in sera of aspergillosis patients
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 40:4382-7. 2002
    ..In conclusion, the combined Afmp1p antibody and antigen tests are highly sensitive and specific for A. fumigatus invasive aspergillosis...
  56. pmc Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 80:7136-45. 2006
    ..g., pol or helicase) and another from HE to N (e.g., spike or N). Further studies will delineate whether the ATR is useful for the molecular typing of CoV HKU1...
  57. pmc Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 79:884-95. 2005
    ..13 x 10(6) copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans...
  58. ncbi request reprint Granulicatella adiacens and Abiotrophia defectiva bacteraemia characterized by 16S rRNA gene sequencing
    Patrick Chiu Yat Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Med Microbiol 52:137-40. 2003
    ..defectiva. 16S rRNA gene sequencing is the technique of choice for identifying G. adiacens and A. defectiva, and early surgical intervention should be considered when G. adiacens endocarditis is diagnosed...
  59. pmc Usefulness of the MicroSeq 500 16S ribosomal DNA-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 41:1996-2001. 2003
    ....
  60. ncbi request reprint Polymorphisms of CCR5 gene in a southern Chinese population and their effects on disease progression in HIV infections
    Bo Jian Zheng
    HIV Research Laboratories, Department of Microbiology, The University of Hong Kong, Hong Kong, China
    AIDS 16:2480-2. 2002
  61. doi request reprint Phaeoacremonium parasiticum invasive infections and airway colonization characterized by agar block smear and ITS and ╬▓-tubulin gene sequencing
    Kelvin K W To
    Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China
    Diagn Microbiol Infect Dis 74:190-7. 2012
    ..parasiticum. Cases of P. parasiticum infections should be confirmed by a polyphasic approach using morphologic characterization and ITS and β-tubulin gene sequencing...
  62. pmc Recent transmission of a novel alphacoronavirus, bat coronavirus HKU10, from Leschenault's rousettes to pomona leaf-nosed bats: first evidence of interspecies transmission of coronavirus between bats of different suborders
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong
    J Virol 86:11906-18. 2012
    ..This study provided the first evidence for interspecies transmission of coronavirus between bats of different suborders...
  63. pmc Ecoepidemiology and complete genome comparison of different strains of severe acute respiratory syndrome-related Rhinolophus bat coronavirus in China reveal bats as a reservoir for acute, self-limiting infection that allows recombination events
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Research Centre of Infection and Immunology, Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong
    J Virol 84:2808-19. 2010
    ..Such frequent recombination, coupled with rapid evolution especially in ORF7b/ORF8 region, in these animals may have accounted for the cross-species transmission and emergence of SARS...
  64. doi request reprint Identification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirus
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    J Gen Virol 93:1573-82. 2012
    ..Although the pathogenicity of FBoV and CBoV remains to be determined, their presence in different host tissues suggested wide tissue tropism...
  65. doi request reprint Detection of human rhinovirus C in fecal samples of children with gastroenteritis
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    J Clin Virol 53:290-6. 2012
    ..Despite recent discovery of the novel human rhinovirus species, HRV-C, little is known about the association of HRV-C in diseases other than respiratory tract infections...
  66. pmc Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats
    Susanna K P Lau
    Department of Microbiology, Research Centre of Infection and Immunology, State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
    Proc Natl Acad Sci U S A 102:14040-5. 2005
    ..Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals...
  67. ncbi request reprint Characterization of a novel insertion sequence, IS Bp1, in Burkholderia pseudomallei
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    Arch Microbiol 177:267-73. 2002
    ..Using several insertion sequences and a combination of insertion-sequence-based and non-insertion-sequence-based methods such as ribotyping will probably increase the discriminatory power of molecular typing in B. pseudomallei...
  68. ncbi request reprint Diagnostic application of genotypic identification of mycobacteria
    Wing Cheong Yam
    Centre of Infection and Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, China
    J Med Microbiol 55:529-36. 2006
    ....
  69. ncbi request reprint Seasonal and tissue distribution of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, in retail freshwater fish in Hong Kong
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Int J Food Microbiol 113:62-6. 2007
    ..Proper handling of freshwater fish for cooking, especially the gills and gut, is recommended to prevent acquisition of L. hongkongensis, and other freshwater fish related infections...
  70. ncbi request reprint Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Med Microbiol 53:1247-53. 2004
    ..Further studies to look for specific virulence factors are warranted...
  71. pmc Human parainfluenza virus 4 outbreak and the role of diagnostic tests
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital
    J Clin Microbiol 43:4515-21. 2005
    ..To better define the epidemiology and clinical spectrum of disease of HPIV-4 infections, HPIV-4 should be included in the routine panels of respiratory virus detection on respiratory specimens...
  72. pmc False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypep
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 42:5885-8. 2004
    ..None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay...
  73. ncbi request reprint "Streptococcus milleri" endocarditis caused by Streptococcus anginosus
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Diagn Microbiol Infect Dis 48:81-8. 2004
    ..Infective endocarditis should be actively looked for in cases of monomicrobial S. anginosus bacteremia, especially if the organism is recovered in multiple blood cultures...
  74. ncbi request reprint Laribacter hongkongensis: a potential cause of infectious diarrhea
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, China
    Diagn Microbiol Infect Dis 47:551-6. 2003
    ..More extensive epidemiologic studies should be carried out to ascertain the causative association between L. hongkongensis and diarrhea and to define the reservoir and modes of transmission of L. hongkongensis...
  75. ncbi request reprint Thermo-tolerant Campylobacter fetus bacteraemia identified by 16S ribosomal RNA gene sequencing: an emerging pathogen in immunocompromised patients
    Patrick C Y Woo
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital and HKU Pasteur Research Center, Hong Kong
    J Med Microbiol 51:740-6. 2002
    ..jejuni in the past 5 years in Hong Kong, thermo-tolerant C fetus may be an emerging pathogen in immunocompromised patients in the years to come...
  76. ncbi request reprint Identification of novel small-molecule inhibitors of severe acute respiratory syndrome-associated coronavirus by chemical genetics
    Richard Y Kao
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Chem Biol 11:1293-9. 2004
    ....
  77. pmc Isolation and characterization of a novel Betacoronavirus subgroup A coronavirus, rabbit coronavirus HKU14, from domestic rabbits
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong
    J Virol 86:5481-96. 2012
    ..Antibody against RbCoV was detected in 20 (67%) of 30 rabbit sera tested by an N-protein-based Western blot assay, whereas neutralizing antibody was detected in 1 of these 20 rabbits...
  78. pmc Natural occurrence and characterization of two internal ribosome entry site elements in a novel virus, canine picodicistrovirus, in the picornavirus-like superfamily
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong
    J Virol 86:2797-808. 2012
    ..Based on our results, we have proposed a novel species, canine picodicistrovirus (CPDV), to describe this novel member of the picornavirus-like superfamily, which could represent a novel family of viruses...
  79. pmc Discovery and genomic characterization of a novel ovine partetravirus and a new genotype of bovine partetravirus
    Herman Tse
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    PLoS ONE 6:e25619. 2011
    ..Results from the present study provide further support to the classification of partetraviruses as a separate genus in Parvovirinae...
  80. pmc Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
    Jade L L Teng
    Department of Microbiology, University of Hong Kong, Hong Kong
    J Clin Microbiol 43:919-22. 2005
    ..Comparing the pulsed-field gel electrophoresis patterns of fish and patient isolates revealed that most patient isolates were clustered together, suggesting that some clones could be more virulent...
  81. pmc Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E
    Bone S F Tang
    Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Virol 79:6180-93. 2005
    ..These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes...
  82. doi request reprint A regulatory polymorphism in interferon-gamma receptor 1 promoter is associated with the susceptibility to chronic hepatitis B virus infection
    Jie Zhou
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, Hong Kong SAR, China
    Immunogenetics 61:423-30. 2009
    ..We conclude that a functional -56C/T SNP in IFNGR1 promoter is associated with the clinical outcome of HBV infection in this Chinese population...
  83. doi request reprint High diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong, China
    FEBS J 277:3750-8. 2010
    ..marneffei and 6 min for the alb1 knockdown mutant of P. marneffei, implying that the melanin-biosynthesis gene cluster contributed to virulence through decreased susceptibility to killing by hydrogen peroxide...
  84. pmc Clinical and molecular epidemiology of human parainfluenza virus 4 infections in hong kong: subtype 4B as common as subtype 4A
    Susanna K P Lau
    Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong
    J Clin Microbiol 47:1549-52. 2009
    ..Patients with HPIV4 infection were mainly young children and immunocompromised adults. In contrast to the reported predominance of HPIV4A infection, molecular subtyping revealed that 15 (44%) cases were caused by HPIV4B...
  85. doi request reprint Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection
    Che Man Chan
    Department of Microbiology, The University of Hong Kong, Hong Kong Special Administration Region, Hong Kong
    Exp Biol Med (Maywood) 233:1527-36. 2008
    ..The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle...
  86. pmc Analysis of a viridans group strain reveals a case of bacteremia due to lancefield group G alpha-hemolytic Streptococcus dysgalactiae subsp equisimilis in a patient with pyomyositis and reactive arthritis
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong
    J Clin Microbiol 41:613-8. 2003
    ..dysgalactiae subsp. equisimilis or a novel type of S. dysgalactiae. The present case showed that group G alpha-hemolytic S. dysgalactiae subsp. equisimilis can be associated with serious invasive infection and poststreptococcal sequelae...
  87. pmc Life-threatening invasive Helcococcus kunzii infections in intravenous-drug users and ermA-mediated erythromycin resistance
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 43:6205-8. 2005
    ..Gram smears of both H. kunzii isolates showed a mixture of gram-positive and gram-negative cocci. The isolate from the first patient, resistant to erythromycin and clindamycin, possessed an ermA gene...
  88. ncbi request reprint Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong
    FEBS Lett 580:3409-16. 2006
    ..We speculate that P. marneffei can potentially be a heterothallic fungus that does not switch mating type...
  89. pmc AFMP2 encodes a novel immunogenic protein of the antigenic mannoprotein superfamily in Aspergillus fumigatus
    Ken T K Chong
    Department of Microbiology, University of Hong Kong, Hong Kong
    J Clin Microbiol 42:2287-91. 2004
    ..fumigatus infections suggest that Afmp2p is a good candidate for complementing Afmp1p in serodiagnosis of A. fumigatus infections...
  90. pmc Internal transcribed spacer region sequence heterogeneity in Rhizopus microsporus: implications for molecular diagnosis in clinical microbiology laboratories
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong
    J Clin Microbiol 48:208-14. 2010
    ..When thick bands and double peaks are observed during PCR sequencing of a gene target, such a strain should be sent to reference laboratories proficient in molecular technologies for further identification and/or genotyping...
  91. doi request reprint Leptin mediates the pathogenesis of severe 2009 pandemic influenza A(H1N1) infection associated with cytokine dysregulation in mice with diet-induced obesity
    Anna J X Zhang
    Carol Yu Centre for Infection and Division of Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam Rd, Pokfulam, Hong Kong SAR, China
    J Infect Dis 207:1270-80. 2013
    ..The mechanism for severe lung injury in obese patients and the specific treatment strategy remain elusive...
  92. pmc Clinical spectrum of exophiala infections and a novel Exophiala species, Exophiala hongkongensis
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    J Clin Microbiol 51:260-7. 2013
    ..We propose the new species Exophiala hongkongensis to describe this novel fungus...
  93. pmc Identification of a novel feline picornavirus from the domestic cat
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, Hong Kong
    J Virol 86:395-405. 2012
    ..Further studies are important to understand the pathogenicity, epidemiology, and genetic evolution of FePV in these common pet animals...
  94. ncbi request reprint Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
    Patrick C Y Woo
    Department of Microbiology, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital Compound, Pokfulam Road, Hong Kong
    FEMS Microbiol Lett 252:57-65. 2005
    ..This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis...
  95. pmc Development of a multi-locus sequence typing scheme for Laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveler's diarrhea
    Patrick C Y Woo
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China
    BMC Microbiol 9:21. 2009
    ..hongkongensis. In this study, a multilocus sequence typing (MLST) system was developed for L. hongkongensis. The system was used to characterize 146 L. hongkongensis isolates, including 39 from humans and 107 from fish...
  96. pmc Complete genome analysis of three novel picornaviruses from diverse bat species
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 85:8819-28. 2011
    ..This report describes the discovery and complete genome analysis of three picornaviruses in bats, and their presence in diverse bat genera/species suggests the ability to cross the species barrier...
  97. pmc Automated pangenomic analysis in target selection for PCR detection and identification of bacteria by use of ssGeneFinder Webserver and its application to Salmonella enterica serovar Typhi
    Chi Chun Ho
    Department of Microbiology, The University of Hong Kong, Hong Kong, China
    J Clin Microbiol 50:1905-11. 2012
    ..Typhi. These and other validated targets should be applicable to the identification and direct PCR detection of bacterial pathogens from uncultured, mixed, and environmental samples...
  98. ncbi request reprint Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Hong Kong
    Virology 367:428-39. 2007
    ..These suggest a common evolutionary origin in the spike protein of bat-CoV HKU2, bat-SARS-CoV, and SARS-CoV...
  99. doi request reprint D225G mutation in hemagglutinin of pandemic influenza H1N1 (2009) virus enhances virulence in mice
    Bojian Zheng
    Department of Microbiology, The University of Hong Kong, Pokfulam, China
    Exp Biol Med (Maywood) 235:981-8. 2010
    ..The only consistent mutation in the genomes of viral clones obtained from dying mice was D225G substitution...
  100. pmc Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay
    Susanna K P Lau
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Clin Microbiol 42:2884-9. 2004
    ..Further studies on serial clinical specimens should reveal the duration of nucleocapsid protein shedding and may reveal a higher detection rate in SARS patients...
  101. doi request reprint Is the discovery of the novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) the beginning of another SARS-like pandemic?
    Jasper F W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong
    J Infect 65:477-89. 2012
    ..Despite the apparently low transmissibility of the virus at this stage, research preparedness against another SARS-like pandemic is an important precautionary strategy...