Leo Lm Poon

Summary

Affiliation: University of Hong Kong
Country: China

Publications

  1. ncbi request reprint The aetiology, origins, and diagnosis of severe acute respiratory syndrome
    L L M Poon
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet Infect Dis 4:663-71. 2004
  2. pmc Prevalence and genetic diversity of coronaviruses in bats from China
    X C Tang
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 80:7481-90. 2006
  3. pmc Rapid genotyping of swine influenza viruses
    Polly W Y Mak
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 17:691-4. 2011
  4. pmc Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 4:e5658. 2009
  5. pmc DNA intercalator stimulates influenza transcription and virus replication
    Olive T W Li
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong
    Virol J 8:120. 2011
  6. pmc Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans
    Stella S F Ng
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    Virol J 5:2. 2008
  7. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
  8. pmc Codon usage bias and the evolution of influenza A viruses. Codon Usage Biases of Influenza Virus
    Emily H M Wong
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, China
    BMC Evol Biol 10:253. 2010
  9. pmc Rapid detection of reassortment of pandemic H1N1/2009 influenza virus
    Leo L M Poon
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong SAR, China
    Clin Chem 56:1340-4. 2010
  10. ncbi request reprint Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays
    Leo L M Poon
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    Clin Chem 50:67-72. 2004

Collaborators

Detail Information

Publications64

  1. ncbi request reprint The aetiology, origins, and diagnosis of severe acute respiratory syndrome
    L L M Poon
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet Infect Dis 4:663-71. 2004
    ..However, the low viral load in the respiratory tract makes early diagnosis of SARS a diagnostic challenge, although improvements in the sensitivity of molecular diagnostic methods continue to be made...
  2. pmc Prevalence and genetic diversity of coronaviruses in bats from China
    X C Tang
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 80:7481-90. 2006
    ..Thus, the findings of this study suggest that bats may play an integral role in the ecology and evolution of coronaviruses...
  3. pmc Rapid genotyping of swine influenza viruses
    Polly W Y Mak
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 17:691-4. 2011
    ....
  4. pmc Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 4:e5658. 2009
    ..These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern...
  5. pmc DNA intercalator stimulates influenza transcription and virus replication
    Olive T W Li
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong
    Virol J 8:120. 2011
    ..This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII(a) in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII(a)) to hyperphosphorylated RNAPII (RNAPII(o))...
  6. pmc Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans
    Stella S F Ng
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    Virol J 5:2. 2008
    ..The observations suggested that, with the cooperation between the homologous 5' and 3'segment-specific sequences, the introduced mutations could specifically enhance the replication of NA and NS vRNA...
  7. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
    ..Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells...
  8. pmc Codon usage bias and the evolution of influenza A viruses. Codon Usage Biases of Influenza Virus
    Emily H M Wong
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, China
    BMC Evol Biol 10:253. 2010
    ..A better understanding of viral evolution and host adaptive responses might help control this disease...
  9. pmc Rapid detection of reassortment of pandemic H1N1/2009 influenza virus
    Leo L M Poon
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong SAR, China
    Clin Chem 56:1340-4. 2010
    ..These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans...
  10. ncbi request reprint Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays
    Leo L M Poon
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    Clin Chem 50:67-72. 2004
    ..We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens...
  11. ncbi request reprint Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR
    Clin Chem 50:1050-2. 2004
  12. pmc Detection of human influenza A viruses by loop-mediated isothermal amplification
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    J Clin Microbiol 43:427-30. 2005
    ..Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test...
  13. ncbi request reprint Early diagnosis of SARS coronavirus infection by real time RT-PCR
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Pokfulam, Hong Kong
    J Clin Virol 28:233-8. 2003
    ..Molecular assays currently available for detection of SARS-coronavirus (SARS-CoV) have low sensitivity during the early stage of the illness...
  14. pmc Identification of a novel coronavirus in bats
    L L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, Special Administrative Region, People s Republic of China
    J Virol 79:2001-9. 2005
    ..In particular, 63% (12 of 19) of fecal samples from Miniopterus pusillus were positive for the virus. These findings suggest that this virus might be commonly circulating in M. pusillus in Hong Kong...
  15. pmc Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    J Clin Microbiol 43:3457-9. 2005
    ..The simpler operation of LAMP might be a possible solution for on-site diagnosis...
  16. ncbi request reprint Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification
    Leo L M Poon
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    Clin Chem 52:303-6. 2006
    ..The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum...
  17. ncbi request reprint Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS)
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    Clin Chem 49:953-5. 2003
  18. pmc Viral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
    Leo L M Poon
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Clin Virol 52:146-50. 2011
    ..There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families...
  19. ncbi request reprint A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China
    J Clin Virol 30:214-7. 2004
    ....
  20. ncbi request reprint The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)
    F Y Zeng
    Department of Zoology, University of Hong Kong, Hong Kong, SAR China
    Exp Biol Med (Maywood) 228:866-73. 2003
    ..Together, these results confirm that HK-39 is a novel and distinct member of the Coronaviridae family, with unknown origin. The completion of the genomic sequence of the virus will assist in tracing its origin...
  21. doi request reprint Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats
    D K W Chu
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    J Gen Virol 89:1282-7. 2008
    ..These findings highlight that co-infections of some coronaviruses might be common events in nature. The biological basis for the host restriction of bat coronaviruses, however, is yet to be determined...
  22. pmc H5N1 influenza: a protean pandemic threat
    Y Guan
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, People s Republic of China
    Proc Natl Acad Sci U S A 101:8156-61. 2004
    ..The acquisition by the viruses of characteristics that enhance virulence in humans and waterfowl and their potential for wider distribution by infected migrating birds are causes for renewed pandemic concern...
  23. ncbi request reprint Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome
    Y Guan
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet 363:99-104. 2004
    ..We have used molecular epidemiology to define the patterns of spread of the virus in Hong Kong and beyond...
  24. pmc Detection of a novel and highly divergent coronavirus from asian leopard cats and Chinese ferret badgers in Southern China
    B Q Dong
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 81:6920-6. 2007
    ..This study demonstrates the importance of systematic virological surveillance in market animals for understanding the evolution and emergence of viruses with infectious potential...
  25. ncbi request reprint Coronavirus as a possible cause of severe acute respiratory syndrome
    J S M Peiris
    Department of Microbiology and Pathology, Queen Mary Hospital, University of Hong Kong, Special Administrative Region, Hong Kong, China
    Lancet 361:1319-25. 2003
    ..An outbreak of severe acute respiratory syndrome (SARS) has been reported in Hong Kong. We investigated the viral cause and clinical presentation among 50 patients...
  26. ncbi request reprint Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People s Republic of China
    Science 302:276-8. 2003
    ..The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known...
  27. pmc Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings
    C M Chu
    Department of Microbiology and Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong
    Thorax 59:252-6. 2004
    ....
  28. ncbi request reprint Coronaviruses in bent-winged bats (Miniopterus spp.)
    D K W Chu
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    J Gen Virol 87:2461-6. 2006
    ....
  29. ncbi request reprint Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study
    J S M Peiris
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong, Special Administrative Region, China
    Lancet 361:1767-72. 2003
    ..We investigated the temporal progression of the clinical, radiological, and virological changes in a community outbreak of severe acute respiratory syndrome (SARS)...
  30. pmc Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63
    K H Chan
    Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, University Pathology Building, Pokfulam Rd, Hong Kong, SAR, People s Republic of China
    Clin Diagn Lab Immunol 12:1317-21. 2005
    ..There is a need for awareness of cross-reactive antibody responses between coronaviruses when interpreting IF serology...
  31. pmc Establishment of influenza A virus (H6N1) in minor poultry species in southern China
    C L Cheung
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Li Ka Shing Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong, SAR, China
    J Virol 81:10402-12. 2007
    ..These results provide further evidence of the pivotal role of the live poultry market system of southern China in generating increased genetic diversity in influenza viruses in this region...
  32. ncbi request reprint Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods
    W C Yam
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong SAR, PR China
    J Clin Virol 33:19-24. 2005
    ..However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease...
  33. pmc Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus
    W C Yam
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
    J Clin Microbiol 41:4521-4. 2003
    ..Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV...
  34. ncbi request reprint Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong SAR, China
    Avian Dis 47:911-3. 2003
    ..The internal gene constellation is also different from H5N1/97, but these variants have the potential for further reassortment events that may allow the interspecies transmission of the virus...
  35. pmc Novel astroviruses in insectivorous bats
    D K W Chu
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    J Virol 82:9107-14. 2008
    ..These findings are likely to provide new insights into the ecology and evolution of astroviruses and reinforce the role of bats as a reservoir of viruses with potential to pose a zoonotic threat to human health...
  36. doi request reprint One step closer to universal influenza epitopes
    Olive Tw Li
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    Expert Rev Anti Infect Ther 7:687-90. 2009
    ..Here, we review some of the recent developments in this area...
  37. pmc Reassortment of pandemic H1N1/2009 influenza A virus in swine
    D Vijaykrishna
    State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong Special Administrative Region, China
    Science 328:1529. 2010
    ..Continued reassortment of H1N1/2009 with swine influenza viruses could produce variants with transmissibility and altered virulence for humans. Global systematic surveillance of influenza viruses in swine is warranted...
  38. ncbi request reprint Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease?
    C Y Cheung
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, SAR, Hong Kong, China
    Lancet 360:1831-7. 2002
    ..The biological basis for the severity of human H5N1 disease has remained unclear. We tested the hypothesis that virus-induced cytokine dysregulation has a role...
  39. ncbi request reprint Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron
    T K W Cheung
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Pokfulam, Hong Kong SAR
    J Gen Virol 86:1447-54. 2005
    ..These results also indicated that the M2 ion-channel protein is critical, but not essential, for virus replication in cell culture. This approach may provide a new way of producing attenuated influenza A virus...
  40. ncbi request reprint Evolution and adaptation of H5N1 influenza virus in avian and human hosts in Indonesia and Vietnam
    G J D Smith
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    Virology 350:258-68. 2006
    ..Our study provides insight into the ongoing evolution of H5N1 influenza viruses that are transmitting in diverse avian species and at the interface between avian and human hosts...
  41. doi request reprint Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)
    K H Chan
    Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Clin Virol 45:205-7. 2009
    ..A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic...
  42. ncbi request reprint Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract
    J M Nicholls
    Department of Pathology, University of Hong Kong, Pok Fu Lam Road, Hong Kong SAR, China
    Nat Med 13:147-9. 2007
    ..We now demonstrate that ex vivo cultures of human nasopharyngeal, adenoid and tonsillar tissues can be infected with H5N1 viruses in spite of an apparent lack of these receptors...
  43. ncbi request reprint Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage
    Eric J Snijder
    Department of Medical Microbiology, Leiden University Medical Center, Room L4 34, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands
    J Mol Biol 331:991-1004. 2003
    ..Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV...
  44. ncbi request reprint Inhibition of SARS-associated coronavirus infection and replication by RNA interference
    Ming Liang He
    JAMA 290:2665-6. 2003
  45. ncbi request reprint Serial analysis of the plasma concentration of SARS coronavirus RNA in pediatric patients with severe acute respiratory syndrome
    Enders K O Ng
    Department of Chemical Pathology, Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
    Clin Chem 49:2085-8. 2003
  46. pmc Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples
    Kwok Hung Chan
    Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People s Republic of China
    J Clin Microbiol 46:2195-9. 2008
    ..The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination...
  47. ncbi request reprint Potent inhibition of SARS-associated coronavirus (SCOV) infection and replication by type I interferons (IFN-alpha/beta) but not by type II interferon (IFN-gamma)
    Bojian Zheng
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong
    J Interferon Cytokine Res 24:388-90. 2004
    ..The type I IFN receptor signaling pathway in host cells is mainly involved in the inhibition of SCoV infection and replication. Type I IFNs could be used as potential agents for anti-SARS treatment...
  48. ncbi request reprint Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
    Yuanxiang Zhou
    Department of Botany, University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, PR China
    FEBS Lett 577:345-50. 2004
    ..These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases...
  49. pmc Initial viral load and the outcomes of SARS
    Chung Ming Chu
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, China
    CMAJ 171:1349-52. 2004
    ..Preliminary data suggest that a high viral load of the SARS coronavirus is associated with adverse outcomes in the intensive care unit, but the relation of viral load to survival is unclear...
  50. ncbi request reprint Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong
    J Med Virol 76:435-40. 2005
    ..A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus...
  51. pmc Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
    PLoS Med 3:e27. 2006
    ..To our knowledge, there have been no comprehensive studies investigating viral infection at the cellular level in humans...
  52. pmc Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis
    Chung Y Cheung
    Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong, Special Administrative Region, People s Republic of China
    J Virol 79:7819-26. 2005
    ..The poor induction of IFN-beta, a key component of innate immunity, and the ability of the virus to induce chemokines could explain aspects of the pathogenesis of SARS...
  53. pmc Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants
    Jan Ter Meulen
    Crucell Holland B V, Leiden, Netherlands
    PLoS Med 3:e237. 2006
    ..Combinations of virus-neutralizing, noncompeting mAbs may have these properties...
  54. ncbi request reprint Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
    J Virol Methods 142:218-22. 2007
    ..The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes...
  55. pmc Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 79:884-95. 2005
    ..13 x 10(6) copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans...
  56. ncbi request reprint Biology of influenza a virus
    Timothy K W Cheung
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China
    Ann N Y Acad Sci 1102:1-25. 2007
    ..The viral genome contains eight RNA viral segments and the functions of viral proteins encoded by this genome are described. In addition, the RNA transcription and replication of this virus are reviewed...
  57. ncbi request reprint SARS and other coronaviruses in humans and animals
    Leo L M Poon
    The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Adv Exp Med Biol 581:457-62. 2006
  58. ncbi request reprint Lung pathology of fatal severe acute respiratory syndrome
    John M Nicholls
    Department of Pathology, University of Hong Kong, Hong Kong Special Administrative Region, China
    Lancet 361:1773-8. 2003
    ..Severe acute respiratory syndrome (SARS) is a novel infectious disease with global impact. A virus from the family Coronaviridae has been identified as the cause, but the pathogenesis is still unclear...
  59. ncbi request reprint The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases
    Julian A Tanner
    Department of Biochemistry, Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    J Biol Chem 278:39578-82. 2003
    ..We found a large variation in the apparent strength of binding of different homopolynucleotides, with dT24 binding over 10 times more strongly than dA24 as observed by the apparent Km...
  60. ncbi request reprint Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection
    Vera S F Chan
    Department of Surgery, Hong Kong Jockey Club Clinical Research Center, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, SAR, China
    Nat Genet 38:38-46. 2006
    ..Thus, homozygosity for L-SIGN plays a protective role during SARS infection...
  61. ncbi request reprint Differential DNA methylation between fetus and mother as a strategy for detecting fetal DNA in maternal plasma
    Leo L M Poon
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 48:35-41. 2002
    ..Fetal DNA has been detected in maternal plasma by the use of genetic differences between mother and fetus. We explore the possibility of using epigenetic markers for the specific detection of fetal DNA in maternal plasma...
  62. ncbi request reprint The nsp9 replicase protein of SARS-coronavirus, structure and functional insights
    Geoff Sutton
    Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK
    Structure 12:341-53. 2004
    ..This is confirmed by analytical ultracentrifugation and dynamic light scattering. We show that nsp9 binds RNA and interacts with nsp8, activities that may be essential for its function(s)...
  63. ncbi request reprint Development of a safe neutralization assay for SARS-CoV and characterization of S-glycoprotein
    Dong P Han
    Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Virology 326:140-9. 2004
    ..The high-throughput capacity of this procedure and the safety of using SARS pseudoviruses should make possible large-scale analyses of neutralizing antibody responses against SARS-CoV...
  64. pmc Loop-mediated isothermal amplification for influenza A (H5N1) virus
    Shanthi Jayawardena
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 13:899-901. 2007
    ..The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes...