Yi Guan

Summary

Affiliation: University of Hong Kong
Country: China

Publications

  1. pmc Minimizing the threat of pandemic emergence from avian influenza in poultry systems
    Kim M Pepin
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, SAR, PRC Hong Kong
    BMC Infect Dis 13:592. 2013
  2. pmc Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro
    Suki M Y Lee
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China
    Respir Res 11:147. 2010
  3. pmc Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells
    Michael C W Chan
    Departments of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, PR China
    Respir Res 10:102. 2009
  4. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
  5. doi request reprint The emergence of pandemic influenza viruses
    Yi Guan
    State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China
    Protein Cell 1:9-13. 2010
  6. pmc H5N1 influenza: a protean pandemic threat
    Y Guan
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, People s Republic of China
    Proc Natl Acad Sci U S A 101:8156-61. 2004
  7. ncbi request reprint Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People s Republic of China
    Science 302:276-8. 2003
  8. ncbi request reprint Resistance to anti-influenza agents
    Yi Guan
    Joint Influenza Research Centre SUMC and HKU, Shantou University Medical College, Shantou, Guangdong 515031, China
    Lancet 366:1139-40. 2005
  9. pmc A model to control the epidemic of H5N1 influenza at the source
    Y Guan
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    BMC Infect Dis 7:132. 2007
  10. ncbi request reprint Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia
    Patrick C Y Woo
    Division of Infectious Disease, Department of Microbiology, The University of Hong Kong, Hong Kong, China
    J Infect Dis 192:1898-907. 2005

Detail Information

Publications164 found, 100 shown here

  1. pmc Minimizing the threat of pandemic emergence from avian influenza in poultry systems
    Kim M Pepin
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, SAR, PRC Hong Kong
    BMC Infect Dis 13:592. 2013
    ..The ongoing human outbreak of avian H7N9 in China highlights the need for increased surveillance and control in these live-bird markets (LBMs)...
  2. pmc Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro
    Suki M Y Lee
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China
    Respir Res 11:147. 2010
    ..However a comprehensive gene expression profile of pdmH1N1 in relevant primary human cells in vitro has not been reported. Type I alveolar epithelial cells are a key target cell in pdmH1N1 pneumonia...
  3. pmc Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells
    Michael C W Chan
    Departments of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, PR China
    Respir Res 10:102. 2009
    ..These are polarized cells and the polarity of influenza virus entry and egress as well as the secretion of cytokines and chemokines from the virus infected cells are likely to be central to the pathogenesis of human H5N1 disease...
  4. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
    ..Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells...
  5. doi request reprint The emergence of pandemic influenza viruses
    Yi Guan
    State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China
    Protein Cell 1:9-13. 2010
    ..In this review, we summarise the recent literature that describes the evolutionary pathway of the pandemic viruses. We also discuss the implications of these findings on the early detection and control of future pandemics...
  6. pmc H5N1 influenza: a protean pandemic threat
    Y Guan
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, People s Republic of China
    Proc Natl Acad Sci U S A 101:8156-61. 2004
    ..The acquisition by the viruses of characteristics that enhance virulence in humans and waterfowl and their potential for wider distribution by infected migrating birds are causes for renewed pandemic concern...
  7. ncbi request reprint Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People s Republic of China
    Science 302:276-8. 2003
    ..The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known...
  8. ncbi request reprint Resistance to anti-influenza agents
    Yi Guan
    Joint Influenza Research Centre SUMC and HKU, Shantou University Medical College, Shantou, Guangdong 515031, China
    Lancet 366:1139-40. 2005
  9. pmc A model to control the epidemic of H5N1 influenza at the source
    Y Guan
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    BMC Infect Dis 7:132. 2007
    ..Systematic surveillance in southern China showed that ducks and geese are the primary reservoirs which transmit the virus to chickens, minor poultry and even migratory birds...
  10. ncbi request reprint Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia
    Patrick C Y Woo
    Division of Infectious Disease, Department of Microbiology, The University of Hong Kong, Hong Kong, China
    J Infect Dis 192:1898-907. 2005
    ..Recently, we described the discovery of a novel group 2 coronavirus, coronavirus HKU1 (CoV-HKU1), from a patient with pneumonia. However, the clinical and molecular epidemiological features of CoV-HKU1-associated pneumonia are unknown...
  11. doi request reprint Multiannual patterns of influenza A transmission in Chinese live bird market systems
    Kim M Pepin
    International Institution of Infection and Immunity, Shantou University Medical College, Shantou, China Colorado State University, Fort Collins, CO, USA
    Influenza Other Respir Viruses 7:97-107. 2013
    ....
  12. doi request reprint Long-term evolution and transmission dynamics of swine influenza A virus
    Dhanasekaran Vijaykrishna
    State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China
    Nature 473:519-22. 2011
    ..Our findings indicate that increased antigenic drift is associated with reassortment events and offer insights into the emergence of influenza viruses with epidemic potential in swine and humans...
  13. pmc Characterization of avian influenza viruses A (H5N1) from wild birds, Hong Kong, 2004-2008
    Gavin J D Smith
    State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 15:402-7. 2009
    ..3.4 viruses are established in poultry in Asia, the emergence of clade 2.3.2 viruses in nonpasserine birds from Hong Kong, Japan, and Russia raises the possibility that this virus lineage may have become established in wild birds...
  14. pmc Rapid detection of reassortment of pandemic H1N1/2009 influenza virus
    Leo L M Poon
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong SAR, China
    Clin Chem 56:1340-4. 2010
    ..These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans...
  15. pmc Swine influenza in Sri Lanka
    Harsha K K Perera
    University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 19:481-4. 2013
    ..Genetic and epidemiologic analyses of human and swine influenza viruses indicated 2 events of A(H1N1)pdm09 virus spillover from humans to pigs...
  16. pmc Antigenic profile of avian H5N1 viruses in Asia from 2002 to 2007
    Wai Lan Wu
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, People s Republic of China
    J Virol 82:1798-807. 2008
    ..These results provide a comprehensive antigenic profile for H5N1 virus strains circulating in recent years and will facilitate the recognition of emerging antigenic variants of H5N1 virus and aid in the selection of vaccine strains...
  17. pmc Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
    Patrick C Y Woo
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    J Virol 79:884-95. 2005
    ..13 x 10(6) copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans...
  18. pmc Establishment and lineage replacement of H6 influenza viruses in domestic ducks in southern China
    Kai Huang
    International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong, China
    J Virol 86:6075-83. 2012
    ....
  19. pmc Emergence and dissemination of a swine H3N2 reassortant influenza virus with 2009 pandemic H1N1 genes in pigs in China
    Xiaohui Fan
    Department of Microbiology, Guangxi Medical University, Nanning, China
    J Virol 86:2375-8. 2012
    ..This is the first evidence that swine reassortant viruses with pdm/09-like genes may have become established in the field, altering the landscape of human and swine influenza...
  20. pmc 2009 pandemic H1N1 influenza virus replicates in human lung tissues
    Jinxia Zhang
    International Institute of Infection and Immunity, First Affiliated Hospital, Shantou University Medical College, Guangdong, China
    J Infect Dis 201:1522-6. 2010
    ..Double-immunostaining for viral antigens and cellular markers indicated that pandemic H1N1 influenza virus replicates in type 2 alveolar epithelial cells...
  21. ncbi request reprint Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong
    J Med Virol 76:435-40. 2005
    ..A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus...
  22. pmc Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 4:e5658. 2009
    ..These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern...
  23. doi request reprint Molecular detection of human H7N9 influenza A virus causing outbreaks in China
    Chloe K S Wong
    Centre of Influenza Research and School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Clin Chem 59:1062-7. 2013
    ..Molecular tests for rapid detection of this virus are urgently needed...
  24. pmc Rapid genotyping of swine influenza viruses
    Polly W Y Mak
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 17:691-4. 2011
    ....
  25. pmc Dating the emergence of pandemic influenza viruses
    Gavin J D Smith
    Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Proc Natl Acad Sci U S A 106:11709-12. 2009
    ....
  26. pmc Gene flow and competitive exclusion of avian influenza A virus in natural reservoir hosts
    Justin Bahl
    State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    Virology 390:289-97. 2009
    ..These results also suggest that viral competition for host resources may be an important mechanism in disease emergence...
  27. doi request reprint Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China
    Y H Connie Leung
    Center of Influenza Research, School of Public Health and State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region
    Vaccine 31:3536-42. 2013
    ..This highlights the need for continued surveillance and monitoring of vaccine induced immunity, with experimental vaccine challenge studies being done where indicated...
  28. pmc The genesis and source of the H7N9 influenza viruses causing human infections in China
    Tommy Tsan Yuk Lam
    Joint Influenza Research Centre SUMC HKU, Shantou University Medical College, Shantou 515041, China
    Nature 502:241-4. 2013
    ..The continuing prevalence of H7 viruses in poultry could lead to the generation of highly pathogenic variants and further sporadic human infections, with a continued risk of the virus acquiring human-to-human transmissibility. ..
  29. doi request reprint H5N1 influenza virus-induced mediators upregulate RIG-I in uninfected cells by paracrine effects contributing to amplified cytokine cascades
    Kenrie P Y Hui
    Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China
    J Infect Dis 204:1866-78. 2011
    ..Thus, sensitizing neighboring cells by upregulation of RIG-I contributes to the amplified cytokine cascades during H5N1 infection...
  30. pmc Novel reassortment of Eurasian avian-like and pandemic/2009 influenza viruses in swine: infectious potential for humans
    Huachen Zhu
    International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong, China
    J Virol 85:10432-9. 2011
    ..Immunization against the 2009 pandemic virus gave only partial protection to ferrets. The continuing prevalence of the pdm/09 virus in pigs could lead to the genesis of novel swine reassortant viruses with the potential to infect humans...
  31. pmc Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis
    Chung Y Cheung
    Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong, Special Administrative Region, People s Republic of China
    J Virol 79:7819-26. 2005
    ..The poor induction of IFN-beta, a key component of innate immunity, and the ability of the virus to induce chemokines could explain aspects of the pathogenesis of SARS...
  32. pmc Reassortment events among swine influenza A viruses in China: implications for the origin of the 2009 influenza pandemic
    Tommy Tsan Yuk Lam
    International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong, China
    J Virol 85:10279-85. 2011
    ..Despite frequent reassortment events between TR and EA lineage viruses in China, evidence for the genesis of the 2009 pandemic virus in pigs in this region is still absent...
  33. pmc Detection of novel astroviruses in urban brown rats and previously known astroviruses in humans
    Daniel K W Chu
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR
    J Gen Virol 91:2457-62. 2010
    ..These rat astroviruses were phylogenetically related to recently discovered human astroviruses MLB1 and MLB2, suggesting that the MLB viruses and these novel rat astroviruses may share a common ancestor...
  34. pmc Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds
    Peter P Cheung
    State Key Laboratory for Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, People s Republic of China
    J Clin Virol 46:90-3. 2009
    ..Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value...
  35. ncbi request reprint A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Pokfulam, Hong Kong SAR, PR China
    J Clin Virol 30:214-7. 2004
    ....
  36. doi request reprint Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice
    Huachen Zhu
    International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong 515031, PR China
    Virology 401:1-5. 2010
    ....
  37. pmc Evolutionary dynamics and emergence of panzootic H5N1 influenza viruses
    Dhanasekaran Vijaykrishna
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Special Administrative Region, China
    PLoS Pathog 4:e1000161. 2008
    ....
  38. doi request reprint Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic
    Gavin J D Smith
    State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    Nature 459:1122-5. 2009
    ..Our results highlight the need for systematic surveillance of influenza in swine, and provide evidence that the mixing of new genetic elements in swine can result in the emergence of viruses with pandemic potential in humans...
  39. doi request reprint Identification of influenza A nucleoprotein as an antiviral target
    Richard Y Kao
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Nat Biotechnol 28:600-5. 2010
    ..Our results demonstrate that viral NP is a valid target for the development of small-molecule therapies...
  40. ncbi request reprint Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays
    Leo L M Poon
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    Clin Chem 50:67-72. 2004
    ..We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens...
  41. pmc Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    J Clin Microbiol 43:3457-9. 2005
    ..The simpler operation of LAMP might be a possible solution for on-site diagnosis...
  42. pmc Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein
    Pui Wang
    Department of Microbiology, The University of Hong Kong, Pokfulam, People s Republic of China
    J Virol 83:7850-61. 2009
    ..These results suggest that NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP...
  43. doi request reprint History of Swine influenza viruses in Asia
    Huachen Zhu
    International Institute of Infection and Immunity, Shantou University Medical College, Guangdong, China
    Curr Top Microbiol Immunol 370:57-68. 2013
    ..Understanding the long-term evolution and history of SIV in Asia would provide insights into the emergence of influenza viruses with epidemic potential in swine and humans...
  44. pmc Characterization of a novel gyrovirus in human stool and chicken meat
    Daniel K W Chu
    Centre for Influenza Research and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region
    J Clin Virol 55:209-13. 2012
    ..Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens...
  45. pmc A novel group of avian astroviruses in wild aquatic birds
    Daniel K W Chu
    State Key Laboratory of Emerging Infectious Diseases, Centre for Influenza Research and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, People s Republic of China
    J Virol 86:13772-8. 2012
    ..Our results also reveal that different groups of astroviruses might have difference host ranges. This study has expanded our understanding regarding avastrovirus ecology...
  46. pmc Avian influenza and ban on overnight poultry storage in live poultry markets, Hong Kong
    Y H Connie Leung
    Li Ka Shing Faculty of Medicine School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 18:1339-41. 2012
    ..A ban on keeping live poultry overnight in these markets reduced virus isolation rates by 84% in chickens (p = 0.006) and 100% (p = 0.01) in minor poultry...
  47. ncbi request reprint Potent inhibition of SARS-associated coronavirus (SCOV) infection and replication by type I interferons (IFN-alpha/beta) but not by type II interferon (IFN-gamma)
    Bojian Zheng
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong
    J Interferon Cytokine Res 24:388-90. 2004
    ..The type I IFN receptor signaling pathway in host cells is mainly involved in the inhibition of SCoV infection and replication. Type I IFNs could be used as potential agents for anti-SARS treatment...
  48. ncbi request reprint Early diagnosis of SARS coronavirus infection by real time RT-PCR
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Pokfulam, Hong Kong
    J Clin Virol 28:233-8. 2003
    ..Molecular assays currently available for detection of SARS-coronavirus (SARS-CoV) have low sensitivity during the early stage of the illness...
  49. pmc Hemagglutinin-neuraminidase balance confers respiratory-droplet transmissibility of the pandemic H1N1 influenza virus in ferrets
    Hui Ling Yen
    Department of Microbiology, HKU Pasteur Research Centre, University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Proc Natl Acad Sci U S A 108:14264-9. 2011
    ..Our results suggest that a unique gene constellation and hemagglutinin-neuraminidase balance play a critical role in acquisition of efficient and sustained human-to-human transmissibility...
  50. pmc Tissue tropism of swine influenza viruses and reassortants in ex vivo cultures of the human respiratory tract and conjunctiva
    Renee W Y Chan
    Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
    J Virol 85:11581-7. 2011
    ..Overall, these findings suggest that ex vivo cultures of the human respiratory tract provide a useful biological model for assessing the human health risk of swine influenza viruses...
  51. ncbi request reprint The aetiology, origins, and diagnosis of severe acute respiratory syndrome
    L L M Poon
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet Infect Dis 4:663-71. 2004
    ..However, the low viral load in the respiratory tract makes early diagnosis of SARS a diagnostic challenge, although improvements in the sensitivity of molecular diagnostic methods continue to be made...
  52. doi request reprint H7N9 Incident, immune status, the elderly and a warning of an influenza pandemic
    Yi Guan
    Joint Influenza Research Centre, SUMC HKU, Shantou University Medical College, Shantou, China
    J Infect Dev Ctries 7:302-7. 2013
    ..Elderly patients greater than 60 years of age accounted for 61% of the cases, indicating that the elderly may be at high risk for severe disease...
  53. ncbi request reprint Distribution of amantadine-resistant H5N1 avian influenza variants in Asia
    Chung Lam Cheung
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong SAR, China
    J Infect Dis 193:1626-9. 2006
    ....
  54. pmc Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003
    K M Butt
    Department of Microbiology, The University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, People s Republic of China
    J Clin Microbiol 43:5760-7. 2005
    ..The continued presence of H9N2 influenza viruses in poultry markets in southern China increases the likelihood of avian-to-human interspecies transmission...
  55. pmc Avian coronavirus in wild aquatic birds
    Daniel K W Chu
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, Chin
    J Virol 85:12815-20. 2011
    ..Our analysis of these avian viral and host mitochondrial DNA sequences also suggests that some, but not all, coronaviruses may have coevolved with birds from the same order...
  56. ncbi request reprint Investigation of outbreaks of highly pathogenic H5N1 avian influenza in waterfowl and wild birds in Hong Kong in late 2002
    Trevor M Ellis
    Tai Lung Veterinary Laboratory, Agriculture Fisheries and Conservation Department, Lin Tong Mei, Sheung Shui, New Territories, Hong Kong SAR, China
    Avian Pathol 33:492-505. 2004
    ..Subsequent virus surveillance showed the outbreaks had been contained...
  57. ncbi request reprint Induction of proinflammatory cytokines in primary human macrophages by influenza A virus (H5N1) is selectively regulated by IFN regulatory factor 3 and p38 MAPK
    Kenrie P Y Hui
    Department of Microbiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong Special Administrative Region, China
    J Immunol 182:1088-98. 2009
    ....
  58. ncbi request reprint Human coronavirus NL63 infection and other coronavirus infections in children hospitalized with acute respiratory disease in Hong Kong, China
    Susan S Chiu
    Department of Paediatrics and Adolescent Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China
    Clin Infect Dis 40:1721-9. 2005
    ....
  59. pmc Loop-mediated isothermal amplification for influenza A (H5N1) virus
    Shanthi Jayawardena
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 13:899-901. 2007
    ..The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes...
  60. ncbi request reprint Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
    J Virol Methods 142:218-22. 2007
    ..The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes...
  61. doi request reprint Analysis of H5N1 avian influenza infections from wild bird surveillance in Hong Kong from January 2006 to October 2007
    Trevor M Ellis
    Tai Lung Veterinary Laboratory, Agriculture Fisheries and Conservation Department, Lin Tong Mei, Sheung Shui, New Territories, Hong Kong, SAR, China
    Avian Pathol 38:107-19. 2009
    ..The use of multiple test methods was important in maintaining the diagnostic sensitivity for detecting avian influenza viruses with high genetic variability...
  62. pmc Seroconversion to pandemic (H1N1) 2009 virus and cross-reactive immunity to other swine influenza viruses
    Ranawaka A P M Perera
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 17:1897-9. 2011
    ..Results indicated that infection with pandemic (H1N1) 2009 broadens cross-reactive immunity to other recent subtype H1 swine viruses...
  63. pmc Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses
    John M Nicholls
    Pathology Department, The University of Hong Kong, Pok Fu Lam, Hong Kong, Hong Kong SAR
    Respir Res 8:73. 2007
    ....
  64. ncbi request reprint Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
    Patrick C Y Woo
    Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong
    Lancet 363:841-5. 2004
    ....
  65. doi request reprint Systemic infection of avian influenza A virus H5N1 subtype in humans
    Zengfeng Zhang
    State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and Research Center of Infection and Immunology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Hum Pathol 40:735-9. 2009
    ..More postmortem studies are needed to elucidate the pathogenesis of this highly fatal zoonotic disease...
  66. pmc Detection of human influenza A viruses by loop-mediated isothermal amplification
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    J Clin Microbiol 43:427-30. 2005
    ..Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test...
  67. pmc Detection of SARS coronavirus in patients with suspected SARS
    Kwok H Chan
    Queen Mary Hospital, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 10:294-9. 2004
    ..Nasopharyngeal aspirates, throat swabs, or sputum samples were the most useful clinical specimens in the first 5 days of illness, but later in the illness viral RNA could be detected more readily in stool specimens...
  68. pmc Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans?
    K S Li
    Joint Influenza Research Centre SUMC and HKU, Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong 515031, P R China
    J Virol 77:6988-94. 2003
    ..Such reassortants may directly or indirectly play a role in the emergence of the next pandemic virus...
  69. ncbi request reprint Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus
    Bo Jion Zheng
    Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Antivir Ther 10:393-403. 2005
    ..Our results revealed novel regions in the spike protein that can be targeted to inhibit viral infection. The peptides identified in this study could be further developed into antiviral drugs...
  70. pmc Two-dimensional antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
    Alexander C K Lai
    Department of Microbiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong
    FEMS Immunol Med Microbiol 64:205-11. 2012
    ..However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis...
  71. pmc Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs
    Maying Tse
    State Key Laboratory of Emerging Infectious Diseases and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
    Clin Vaccine Immunol 19:334-7. 2012
    ....
  72. pmc Temporally structured metapopulation dynamics and persistence of influenza A H3N2 virus in humans
    Justin Bahl
    Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Pokfulam, Hong Kong Special Admnistrative Region, China
    Proc Natl Acad Sci U S A 108:19359-64. 2011
    ..Such complex global migration dynamics may confound control efforts and contribute to the emergence and spread of antigenic variants and drug-resistant viruses...
  73. ncbi request reprint Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission
    Trevor M Ellis
    Tai Lung Veterinary Laboratory, Agriculture Fisheries and Conservation Department, Lin Tong Mei, Sheung Shui, Hong Kong SAR, China
    Avian Pathol 33:405-12. 2004
    ..This provides evidence that H5 vaccine can interrupt virus transmission in a field setting...
  74. ncbi request reprint Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay
    Leo L M Poon
    Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR
    Clin Chem 50:1050-2. 2004
  75. ncbi request reprint Full factorial analysis of Mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 4:e5658. 2009
    ..These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern...
  76. doi request reprint Detection of diverse astroviruses from bats in China
    Hua Chen Zhu
    State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong SAR
    J Gen Virol 90:883-7. 2009
    ..Evidence for varying degrees of host restriction for astroviruses from bats has been found. Phylogenetic analyses also provided insight into the inter-species transmission of Mamastrovirus...
  77. doi request reprint Viral genetic determinants of H5N1 influenza viruses that contribute to cytokine dysregulation
    Ka Pun Mok
    Department of Microbiology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    J Infect Dis 200:1104-12. 2009
    ....
  78. ncbi request reprint Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification
    Leo L M Poon
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    Clin Chem 52:303-6. 2006
    ..The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum...
  79. pmc Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
    Michael C W Chan
    Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
    Am J Pathol 176:1828-40. 2010
    ..These findings are therefore relevant for understanding transmission and therapy...
  80. ncbi request reprint Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus
    Bo Jian Zheng
    Department of Microbiology, Institute of Molecular Biology, Department of Pathology, University of Hong Kong, Hong Kong, China
    Antivir Ther 9:365-74. 2004
    ..This in vitro study will serve as the foundation for development of novel anti-SARS therapeutics...
  81. ncbi request reprint Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong SAR, China
    Avian Dis 47:911-3. 2003
    ..The internal gene constellation is also different from H5N1/97, but these variants have the potential for further reassortment events that may allow the interspecies transmission of the virus...
  82. ncbi request reprint The next influenza pandemic: lessons from Hong Kong
    K F Shortridge
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, China
    J Appl Microbiol 94:70S-79S. 2003
    ..This will enhance public confidence in the investigative process and in steps taken for its safety and health...
  83. pmc Prevalence and genetic diversity of coronaviruses in bats from China
    X C Tang
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 80:7481-90. 2006
    ..Thus, the findings of this study suggest that bats may play an integral role in the ecology and evolution of coronaviruses...
  84. pmc Establishment of an H6N2 influenza virus lineage in domestic ducks in southern China
    K Huang
    State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pok Fu Lam, Hong Kong SAR, China
    J Virol 84:6978-86. 2010
    ..These findings suggest that domestic ducks in southern China mediate the interaction of viruses between different gene pools and facilitate the generation of novel influenza virus variants circulating in poultry...
  85. pmc Influenza-associated hospitalization in a subtropical city
    Chit Ming Wong
    University of Hong Kong, Department of Community Medicine, Hong Kong, Hong Kong Special Administrative Region, China
    PLoS Med 3:e121. 2006
    ..Uniquely, the Hong Kong Special Administrative Region has computerized hospital discharge diagnoses on 95% of total bed days, allowing disease burden for a well-defined population to be accurately assessed...
  86. pmc Avian influenza virus (H5N1): a threat to human health
    J S Malik Peiris
    Department of Microbiology, University Pathology Building, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong, SAR, People s Republic of China
    Clin Microbiol Rev 20:243-67. 2007
    ....
  87. pmc Molecular characterization of in vivo adjuvant activity in ferrets vaccinated against influenza virus
    Yuan Fang
    Division of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, 22 Xinling Road, Shantou, Guangdong 515041, People s Republic of China
    J Virol 84:8369-88. 2010
    ..Taken together, our results better characterize the specific molecular pathways leading to adjuvant activity in different adjuvant-mediated influenza virus vaccinations...
  88. doi request reprint Mutations in influenza virus replication and transcription: detection of amino acid substitutions in hemagglutinin of an avian influenza virus (H1N1)
    Ning Liu
    Z C, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China
    FASEB J 23:3377-82. 2009
    ..The results, for the first time, provided experimental evidence showing differences in AA sequence obtained from direct analysis of viral protein derived from viral genome...
  89. ncbi request reprint Effect of interventions on influenza A (H9N2) isolation in Hong Kong's live poultry markets, 1999-2005
    Eric H Y Lau
    The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    Emerg Infect Dis 13:1340-7. 2007
    ..22) in chickens. Combining 1 rest-day with the removal of quails further reduced virus isolation in chickens but not in minor poultry. However, an additional rest-day each month did not appear to affect isolation rates for either species...
  90. ncbi request reprint H5N1 influenza viruses isolated from geese in Southeastern China: evidence for genetic reassortment and interspecies transmission to ducks
    Y Guan
    Department of Microbiology, The University of Hong Kong, University Pathology Building, Hong Kong SAR, People s Republic of China
    Virology 292:16-23. 2002
    ..For pandemic preparedness, it is important to monitor whether these reassortant viruses have the capacity for interspecies transmission to terrestrial poultry or mammals...
  91. pmc Molecular evolution of H6 influenza viruses from poultry in Southeastern China: prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)-like genes in poultry
    P S Chin
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    J Virol 76:507-16. 2002
    ..the A/Hong Kong/156/97 (H5N1) virus raises the following fundamental questions relevant to influenza pandemic preparedness: could the pathogenic H5N1 virus reemerge and could the H6N1 viruses directly cross the species barrier to mammals?..
  92. doi request reprint Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats
    D K W Chu
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR
    J Gen Virol 89:1282-7. 2008
    ..These findings highlight that co-infections of some coronaviruses might be common events in nature. The biological basis for the host restriction of bat coronaviruses, however, is yet to be determined...
  93. pmc Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China
    J Wang
    International Institute of Infection and Immunity, Shantou University, Shantou, Guangdong 515031, China
    J Virol 82:3405-14. 2008
    ..Poultry trade may be responsible for virus introduction to Vietnam, while the transmission route from Hunan to Indonesia remains unclear...
  94. ncbi request reprint Studies of SARS virus vaccines
    B J Zheng
    Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong
    Hong Kong Med J 14:39-43. 2008
    ..8. Overall, our findings suggest that the inactivated vaccine, RBD-Fc and RBD-rAAV, can be further developed into effective and safe vaccines against SARS and that intranasal vaccination may be the preferred route of administration...
  95. ncbi request reprint Avian influenza in Hong Kong 1997-2002
    L D Sims
    Agriculture, Fisheries, and Conservation Department, 303 Cheung Sha Wan Road, Kowloon, Hong Kong SAR, China
    Avian Dis 47:832-8. 2003
    ..This paper provides an overview and chronology of the events in Hong Kong relating to avian influenza, covering the period from March 1997 to March 2002...
  96. ncbi request reprint Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome
    Y Guan
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet 363:99-104. 2004
    ..We have used molecular epidemiology to define the patterns of spread of the virus in Hong Kong and beyond...
  97. pmc Detection of a novel and highly divergent coronavirus from asian leopard cats and Chinese ferret badgers in Southern China
    B Q Dong
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 81:6920-6. 2007
    ..This study demonstrates the importance of systematic virological surveillance in market animals for understanding the evolution and emergence of viruses with infectious potential...
  98. ncbi request reprint Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS)
    Leo L M Poon
    Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR
    Clin Chem 49:953-5. 2003
  99. doi request reprint Emergence of a novel swine-origin influenza A virus (S-OIV) H1N1 virus in humans
    J S Malik Peiris
    State Key Laboratory for Emerging Infectious Disease and Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    J Clin Virol 45:169-73. 2009
    ..The virus appears to retain the potential to transmit back to swine and thus continued reassortment with swine viruses is a cause for concern...
  100. pmc Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus
    Wenwei Tu
    Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Room L7 58, 7 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Hong Kong
    J Virol 84:6527-35. 2010
    ....
  101. pmc The development and genetic diversity of H5N1 influenza virus in China, 1996-2006
    L Duan
    International Institute of Infection and Immunity, Shantou University Medical College, Shantou 515031, Guangdong, China
    Virology 380:243-54. 2008
    ..Two major replacements of predominant genotypes were also observed: genotype B replaced by Z in 2002 and then genotype Z replaced by the now predominant genotype V in 2005...