Michael S Y Huen

Summary

Affiliation: The University of Hong Kong
Country: China

Publications

  1. pmc Regulation of chromatin architecture by the PWWP domain-containing DNA damage-responsive factor EXPAND1/MUM1
    Michael S Y Huen
    Department of Anatomy, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong SAR
    Mol Cell 37:854-64. 2010
  2. pmc SON is a spliceosome-associated factor required for mitotic progression
    Michael S Y Huen
    Genome Stability Research Laboratory, The University of Hong Kong, Hong Kong, SAR
    Cell Cycle 9:2679-85. 2010
  3. pmc Differential regulation of RNF8-mediated Lys48- and Lys63-based poly-ubiquitylation
    Gabriel Tsz Mei Lok
    Genome Stability Research Laboratory, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong SAR
    Nucleic Acids Res 40:196-205. 2012
  4. pmc The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks
    Shirley M H Sy
    Genome Stability Research Laboratory, The University of Hong Kong, Hong Kong, People s Republic of China, Department of Anatomy, The University of Hong Kong, Hong Kong, People s Republic of China, Center for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, People s Republic of China, Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, GuangDong 510642, China and State Key Laboratory of Brain and Cognitive Science, The University of Hong Kong, Hong Kong, People s Republic of China
    Nucleic Acids Res 41:8572-80. 2013
  5. doi request reprint Loss of ΔNp63α promotes mitotic exit in epithelial cells
    Pok Man Hau
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region
    FEBS Lett 585:2720-6. 2011
  6. pmc Critical roles of ring finger protein RNF8 in replication stress responses
    Shirley M H Sy
    Genome Stability Research Laboratory, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong, China
    J Biol Chem 286:22355-61. 2011
  7. pmc BRCA1 and its toolbox for the maintenance of genome integrity
    Michael S Y Huen
    Department of Anatomy, The University of Hong Kong, Laboratory Block, 21 Sassoon Road, Hong Kong
    Nat Rev Mol Cell Biol 11:138-48. 2010
  8. doi request reprint Epstein-Barr virus BZLF1 protein impairs accumulation of host DNA damage proteins at damage sites in response to DNA damage
    Jie Yang
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Lab Invest 95:937-50. 2015
  9. ncbi request reprint Highly efficient deletion method for the engineering of plasmid DNA with single-stranded oligonucleotides
    Lin Yu Lu
    University of Hong Kong, Pokfulam, Hong Kong, SAR, China
    Biotechniques 44:217-20, 222, 224. 2008
  10. doi request reprint Mequindox induced cellular DNA damage via generation of reactive oxygen species
    Jing Liu
    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    Mutat Res 741:70-5. 2012

Collaborators

Detail Information

Publications17

  1. pmc Regulation of chromatin architecture by the PWWP domain-containing DNA damage-responsive factor EXPAND1/MUM1
    Michael S Y Huen
    Department of Anatomy, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong SAR
    Mol Cell 37:854-64. 2010
    ..Collectively, our study uncovers a chromatin-bound factor that serves an accessory role in coupling damage signaling with chromatin changes in response to DNA damage...
  2. pmc SON is a spliceosome-associated factor required for mitotic progression
    Michael S Y Huen
    Genome Stability Research Laboratory, The University of Hong Kong, Hong Kong, SAR
    Cell Cycle 9:2679-85. 2010
    ..In summary, our study uncovers a specific link between SON and mitosis, and highlights the potential of RNA processing as additional regulatory mechanisms that govern cell proliferation and division...
  3. pmc Differential regulation of RNF8-mediated Lys48- and Lys63-based poly-ubiquitylation
    Gabriel Tsz Mei Lok
    Genome Stability Research Laboratory, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong SAR
    Nucleic Acids Res 40:196-205. 2012
    ..Together, our data suggest that RNF8 regulates K48- and K63-linked poly-ubiquitylation via differential RING-dependent interactions with its E2s UBCH8 and UBC13, respectively...
  4. pmc The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks
    Shirley M H Sy
    Genome Stability Research Laboratory, The University of Hong Kong, Hong Kong, People s Republic of China, Department of Anatomy, The University of Hong Kong, Hong Kong, People s Republic of China, Center for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, People s Republic of China, Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, GuangDong 510642, China and State Key Laboratory of Brain and Cognitive Science, The University of Hong Kong, Hong Kong, People s Republic of China
    Nucleic Acids Res 41:8572-80. 2013
    ..We propose that USP34 promotes a feed-forward loop to enforce ubiquitin signaling at DSBs and highlight critical roles of ubiquitin dynamics in genome stability maintenance. ..
  5. doi request reprint Loss of ΔNp63α promotes mitotic exit in epithelial cells
    Pok Man Hau
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region
    FEBS Lett 585:2720-6. 2011
    ..Overexpression of APC/C coactivator Cdh1 destabilized ΔNp63α. Our results suggest that ΔNp63α level is cell cycle-regulated and may play a role in the regulation of mitotic exit...
  6. pmc Critical roles of ring finger protein RNF8 in replication stress responses
    Shirley M H Sy
    Genome Stability Research Laboratory, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong, China
    J Biol Chem 286:22355-61. 2011
    ..In total, our study highlights the differential requirement for the ubiquitin ligase RNF8 in facilitating repair of replication stress-associated DNA damage...
  7. pmc BRCA1 and its toolbox for the maintenance of genome integrity
    Michael S Y Huen
    Department of Anatomy, The University of Hong Kong, Laboratory Block, 21 Sassoon Road, Hong Kong
    Nat Rev Mol Cell Biol 11:138-48. 2010
    ..Thus, BRCA1 is emerging as the master regulator of the genome through its ability to execute and coordinate various aspects of the DNA damage response...
  8. doi request reprint Epstein-Barr virus BZLF1 protein impairs accumulation of host DNA damage proteins at damage sites in response to DNA damage
    Jie Yang
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    Lab Invest 95:937-50. 2015
    ....
  9. ncbi request reprint Highly efficient deletion method for the engineering of plasmid DNA with single-stranded oligonucleotides
    Lin Yu Lu
    University of Hong Kong, Pokfulam, Hong Kong, SAR, China
    Biotechniques 44:217-20, 222, 224. 2008
    ..Finally, we showed that by optimizing its design, SSO-mediated deletion was efficient enough to make it possible to manipulate plasmids without selectable markers...
  10. doi request reprint Mequindox induced cellular DNA damage via generation of reactive oxygen species
    Jing Liu
    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    Mutat Res 741:70-5. 2012
    ..Taken together, our results suggest that mequindox cytotoxicity is attributable, in part, to its role as a potent inducer of DNA damage via ROS...
  11. pmc Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage
    Jie Chen
    Genome Stability Research Laboratory, The University of Hong Kong, Hong Kong, China
    J Biol Chem 287:27715-22. 2012
    ..Our study unveils RNF169 as a component in DNA damage signal transduction and adds to the complexity of regulatory ubiquitylation in genome stability maintenance...
  12. pmc F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6)
    Jia Liu
    From the Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China and
    J Biol Chem 289:21508-18. 2014
    ..Taken together, our findings uncover a new mechanism of deactivation of MKK6-p38 and substantiate a novel regulatory role of FBXO31 in stress response...
  13. pmc Assembly of checkpoint and repair machineries at DNA damage sites
    Michael S Y Huen
    Department of Anatomy, Centre for Cancer Research, University of Hong Kong, L1 59, Laboratory Block, 21 Sassoon Road, Hong Kong SAR
    Trends Biochem Sci 35:101-8. 2010
    ....
  14. doi request reprint Cell death caused by single-stranded oligodeoxynucleotide-mediated targeted genomic sequence modification
    Chenli Liu
    Department of Biochemistry, The University of Hong Kong, Hong Kong SAR, People s Republic of China
    Oligonucleotides 19:281-6. 2009
    ....
  15. ncbi request reprint Active transcription promotes single-stranded oligonucleotide mediated gene repair
    Michael S Y Huen
    Department of Biochemistry, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
    Biochem Biophys Res Commun 353:33-9. 2007
    ..We found that transcription activation can promote recombinant formation to more than 10-folds whilst it has negligible effect on strand bias. Our findings provide evidence for a role of transcription in SSO-mediated gene repair process...
  16. pmc Overexpression of eIF-5A2 in mice causes accelerated organismal aging by increasing chromosome instability
    Muhan Chen
    Department of Clinical Oncology, Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Hong Kong, China
    BMC Cancer 11:199. 2011
    ..In the present study, we aim to investigate the physiological and pathological effect of eIF-5A2 in an eIF-5A2 transgenic mouse model...
  17. pmc Id1 interacts and stabilizes the Epstein-Barr virus latent membrane protein 1 (LMP1) in nasopharyngeal epithelial cells
    Pok Man Hau
    Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam Hong Kong Special Administrative Region
    PLoS ONE 6:e21176. 2011
    ..N-terminal region of Id1 is required for the interaction with LMP1. Furthermore, binding of Id1 to LMP1 suppressed polyubiquitination of LMP1 and may be involved in stabilization of LMP1 in EBV-infected nasopharyngeal epithelial cells...