Research Topics
| W-P FongSummaryAffiliation: The Chinese University of Hong Kong Country: China Publications
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Detail Information
Publications
Photodynamic therapy inhibits P-glycoprotein mediated multidrug resistance via JNK activation in human hepatocellular carcinoma using the photosensitizer pheophorbide aPatrick Ming Kuen Tang
School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, PR China
Mol Cancer 8:56. 2009..The therapeutic potential of PDT was evaluated in a multidrug resistance (MDR) human hepatoma cell line R-HepG2 with photosensitizer pheophorbide a (Pa)...
Antiquitin, a relatively unexplored member in the superfamily of aldehyde dehydrogenases with diversified physiological functionsW P Fong
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Cell Mol Life Sci 63:2881-5. 2006..The physiological functions of animal antiquitins remain largely elusive, their involvement in a number of human diseases has been implicated...
Intrinsic ribonuclease activities in ribonuclease and ribosome-inactivating proteins from the seeds of bitter gourdW P Fong
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, People s Republic of China
Int J Biochem Cell Biol 32:571-7. 2000..RNase-MC, alpha-momorcharin and beta-momorcharin were separable on Mono-S, indicating that the ribonuclease activities present in the three proteins were distinct entities...
Purification and characterization of two distinct aldehyde-oxidizing enzymes from the liver of black seabreamWing Ping Fong
Department of Biochemistry, The Chinese University of Hong Kong, N T, Hong Kong, Shatin, People s Republic of China
Chem Biol Interact 143:159-67. 2003....
Purification and characterization of grass carp mitochondrial aldehyde dehydrogenaseW Fong
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong
Chem Biol Interact 130:161-71. 2001..5. The K(m) values for acetaldehyde were 0.36 and 0.31 microM at pH 7.5 and 9.5, respectively. Grass carp ALDH also possessed esterase activity which could be activated in the presence of NAD(+)...
Purification, N-terminal sequence determination and enzymatic characterization of antiquitin from the liver of grass carpWing-Man Chan
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
Comp Biochem Physiol B Biochem Mol Biol 136:443-50. 2003..The optimal pH for the oxidation of acetaldehyde was 9.5. At this pH, the Vmax and the Km values for acetaldehyde were 1.95 U/mg and 2.00 mM, respectively...
Glycosylated zinc(II) phthalocyanines as efficient photosensitisers for photodynamic therapy. Synthesis, photophysical properties and in vitro photodynamic activityChi Fung Choi
Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Org Biomol Chem 6:2173-81. 2008..For the mono-glycosylated phthalocyanines, the higher uptake can be attributed to their hydrophilic saccharide units, which increase the amphiphilicity of the macrocycles...
First purification of the antiquitin protein and demonstration of its enzymatic activityWai-Kwan Tang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
FEBS Lett 516:183-6. 2002..The enzyme is highly specific towards NAD+ as the coenzyme and is totally inactive towards NADP+. Maximal enzymatic activity was found at about pH 9-10...
Synthesis and in vitro photodynamic activity of mono-substituted amphiphilic zinc(II) phthalocyaninesPui Chi Lo
Department of Chemistry and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Bioorg Med Chem Lett 17:1073-7. 2007..08microM. The dicationic derivatives have a higher affinity to the cell membrane compared with the non-ionic counterparts...
BAM-SiPc, a novel agent for photodynamic therapy, induces apoptosis in human hepatocarcinoma HepG2 cells by a direct mitochondrial actionJanice C Lai
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
Cancer Biol Ther 5:413-8. 2006....
Preparation and in vitro photodynamic activity of novel silicon(IV) phthalocyanines conjugated to serum albuminsJian-Dong Huang
Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
J Inorg Biochem 100:946-51. 2006..As shown by absorption spectroscopy, the tetracationic phthalocyanine 3 was significantly aggregated in the protein cavity and its photocytotoxicity remained the lowest among the four photosensitizers...
Seabream antiquitin: molecular cloning, tissue distribution, subcellular localization and functional expressionWai-Kwan Tang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
FEBS Lett 579:3759-64. 2005..Bacterial expression of the enzyme was also performed. The purified recombinant protein was demonstrated to exhibit similar kinetic properties as the native enzyme...
Highly photocytotoxic glucosylated silicon(IV) phthalocyanines. Effects of peripheral chloro substitution on the photophysical and photodynamic propertiesPui Chi Lo
Department of Chemistry, Department of Biochemistry, and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
J Med Chem 50:2100-7. 2007..Fluorescence microscopic studies have also revealed that compound 3 has a high and selective affinity to the lysosomes, but not the mitochondria, of HT29 cells...
Effects of peripheral chloro substitution on the photophysical properties and in vitro photodynamic activities of galactose-conjugated silicon(IV) phthalocyaninesPui Chi Lo
Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
ChemMedChem 3:1110-7. 2008..The nonchlorinated analogue exhibits the highest potency and can target the lysosomes of HT29 cells, whereas the monochlorinated counterpart is not localised in the lysosomes...
Synthesis, photophysical properties and in vitro photodynamic activity of axially substituted subphthalocyaninesHu Xu
Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Org Biomol Chem 5:3987-92. 2007..The phenoxy analogues 4 and 5 show a relatively high photostability and are particularly potent towards these cell lines, with IC(50) values down to 0.02 microM...
Photodynamic activity of BAM-SiPc, an unsymmetrical bisamino silicon(IV) phthalocyanine, in tumour-bearing nude miceS C H Leung
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Br J Pharmacol 154:4-12. 2008..The unsymmetrical bisamino silicon(IV) phthalocyanine BAM-SiPc is one of the most potent compounds. In in vitro cell culture, it exhibits high phototoxicity against a number of cancer cell lines...
Pheophorbide a, an active compound isolated from Scutellaria barbata, possesses photodynamic activities by inducing apoptosis in human hepatocellular carcinomaPatrick Ming-Kuen Tang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Cancer Biol Ther 5:1111-6. 2006..Furthermore, in nude mice model, Pa-PDT treatment could reduce the tumor size by 57% after 14 days treatment...
The crystal structure of seabream antiquitin reveals the structural basis of its substrate specificityWai Kwan Tang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
FEBS Lett 582:3090-6. 2008..On the other hand, Arg300 does not have any specific interaction with the alpha-carboxylate group of the substrate, but is important in maintaining the active site conformation...
First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensisAlex Y K Tsoi
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
Biol Chem 385:185-9. 2004..Comparison of this sequence and the molecular mass with those of other protease inhibitors suggests that MCoCl belongs to the potato I inhibitor family...
Pheophorbide a, an active component in Scutellaria barbata, reverses P-glycoprotein-mediated multidrug resistance on a human hepatoma cell line R-HepG2Patrick Ming Kuen Tang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Cancer Biol Ther 6:504-9. 2007..In addition, mechanistic study elucidated that Pa induced cell cycle arrest at G2/M phase and inhibited the expressions of G2/M phase cell cycle regulatory proteins, cyclin-A1 and cdc2 in a dose-dependent manner...
Cloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter regionChi Bun Chan
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Mol Cell Biochem 297:151-60. 2007..Instead, it contains potential binding sites for cell cycle related cis-elements such as CCAAT displacement protein and cell cycle-dependent element/cell cycle genes homology region...
J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha/beta-hydrolase fold superfamilyMing-Hon Yau
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
FEBS Lett 580:1465-71. 2006..Mutations to alanine at these positions were found to completely inactivate the enzyme. These results suggest that J1 acylase is a member of the alpha/beta-hydrolase fold superfamily with a serine-histidine-aspartate catalytic triad...
Immunomodulatory activity of a chymotrypsin inhibitor from Momordica cochinchinensis seedsAlex Yuen-Kam Tsoi
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
J Pept Sci 12:605-11. 2006..Moreover, MCoCI could also suppress the formation of hydrogen peroxide in neutrophils and macrophages. These immunomodulatory effects may explain some of the therapeutic actions of Mubiezhi...
