W-P Fong

Summary

Affiliation: The Chinese University of Hong Kong
Country: China

Publications

  1. pmc Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures
    Yu Ting Saw
    Department of Obstetrics Gynecology and Reproductive Biology, Brigham and Women s Hospital, Boston, MA 02115, USA
    BMC Cancer 12:329. 2012
  2. pmc Photodynamic therapy inhibits P-glycoprotein mediated multidrug resistance via JNK activation in human hepatocellular carcinoma using the photosensitizer pheophorbide a
    Patrick Ming Kuen Tang
    School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, PR China
    Mol Cancer 8:56. 2009
  3. ncbi request reprint Antiquitin, a relatively unexplored member in the superfamily of aldehyde dehydrogenases with diversified physiological functions
    W P Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Cell Mol Life Sci 63:2881-5. 2006
  4. ncbi request reprint Intrinsic ribonuclease activities in ribonuclease and ribosome-inactivating proteins from the seeds of bitter gourd
    W P Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, People s Republic of China
    Int J Biochem Cell Biol 32:571-7. 2000
  5. ncbi request reprint Purification and characterization of grass carp mitochondrial aldehyde dehydrogenase
    W Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong
    Chem Biol Interact 130:161-71. 2001
  6. ncbi request reprint Purification and characterization of two distinct aldehyde-oxidizing enzymes from the liver of black seabream
    Wing Ping Fong
    Department of Biochemistry, The Chinese University of Hong Kong, N T, Hong Kong, Shatin, People s Republic of China
    Chem Biol Interact 143:159-67. 2003
  7. ncbi request reprint Purification, N-terminal sequence determination and enzymatic characterization of antiquitin from the liver of grass carp
    Wing Man Chan
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
    Comp Biochem Physiol B Biochem Mol Biol 136:443-50. 2003
  8. doi request reprint Glycosylated zinc(II) phthalocyanines as efficient photosensitisers for photodynamic therapy. Synthesis, photophysical properties and in vitro photodynamic activity
    Chi Fung Choi
    Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Org Biomol Chem 6:2173-81. 2008
  9. ncbi request reprint First purification of the antiquitin protein and demonstration of its enzymatic activity
    Wai Kwan Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
    FEBS Lett 516:183-6. 2002
  10. ncbi request reprint BAM-SiPc, a novel agent for photodynamic therapy, induces apoptosis in human hepatocarcinoma HepG2 cells by a direct mitochondrial action
    Janice C Lai
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Cancer Biol Ther 5:413-8. 2006

Collaborators

  • Dennis K P Ng
  • Kam Bo Wong
  • W K Liu
  • T B Ng
  • Jun Wang
  • Patrick Ming Kuen Tang
  • Pui Chi Lo
  • Wai Kwan Tang
  • Christopher H K Cheng
  • Jian Dong Huang
  • Chi Bun Chan
  • Janice C Lai
  • Yu Ting Saw
  • Chi Fung Choi
  • S C H Leung
  • Hu Xu
  • Wing Hung Ko
  • Alex Yuen Kam Tsoi
  • Ming Hon Yau
  • Alex Y K Tsoi
  • Wing Man Chan
  • Shu wing Ng
  • Vasilis Vasiliou
  • Hiroshi Tsuda
  • Ross S Berkowitz
  • Junzheng Yang
  • Margit Singh
  • William R Welch
  • David Thompson
  • Shubai Liu
  • Shu Kay Ng
  • Surendra Singh
  • K P Fung
  • P C Lo
  • Sun Shin Cha
  • Yuk Man Lam
  • Elaine Y M Chan
  • Baozhong Zhao
  • Xiong Jie Jiang
  • Crystal M H Chan
  • Jian Yong Liu
  • Wubiao Duan
  • Kwok Pui Fung
  • Paul W K Tsang
  • Stanley C H Leung
  • Yan Mei Chen
  • Ricardo C H Wong

Detail Information

Publications24

  1. pmc Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures
    Yu Ting Saw
    Department of Obstetrics Gynecology and Reproductive Biology, Brigham and Women s Hospital, Boston, MA 02115, USA
    BMC Cancer 12:329. 2012
    ..The purpose of the present study was to determine if the expression and activities of other major ALDH isozymes are associated with human ovarian cancer and ovarian cancer sphere cultures...
  2. pmc Photodynamic therapy inhibits P-glycoprotein mediated multidrug resistance via JNK activation in human hepatocellular carcinoma using the photosensitizer pheophorbide a
    Patrick Ming Kuen Tang
    School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, PR China
    Mol Cancer 8:56. 2009
    ..The therapeutic potential of PDT was evaluated in a multidrug resistance (MDR) human hepatoma cell line R-HepG2 with photosensitizer pheophorbide a (Pa)...
  3. ncbi request reprint Antiquitin, a relatively unexplored member in the superfamily of aldehyde dehydrogenases with diversified physiological functions
    W P Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Cell Mol Life Sci 63:2881-5. 2006
    ..The physiological functions of animal antiquitins remain largely elusive, their involvement in a number of human diseases has been implicated...
  4. ncbi request reprint Intrinsic ribonuclease activities in ribonuclease and ribosome-inactivating proteins from the seeds of bitter gourd
    W P Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, People s Republic of China
    Int J Biochem Cell Biol 32:571-7. 2000
    ..RNase-MC, alpha-momorcharin and beta-momorcharin were separable on Mono-S, indicating that the ribonuclease activities present in the three proteins were distinct entities...
  5. ncbi request reprint Purification and characterization of grass carp mitochondrial aldehyde dehydrogenase
    W Fong
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong
    Chem Biol Interact 130:161-71. 2001
    ..5. The K(m) values for acetaldehyde were 0.36 and 0.31 microM at pH 7.5 and 9.5, respectively. Grass carp ALDH also possessed esterase activity which could be activated in the presence of NAD(+)...
  6. ncbi request reprint Purification and characterization of two distinct aldehyde-oxidizing enzymes from the liver of black seabream
    Wing Ping Fong
    Department of Biochemistry, The Chinese University of Hong Kong, N T, Hong Kong, Shatin, People s Republic of China
    Chem Biol Interact 143:159-67. 2003
    ....
  7. ncbi request reprint Purification, N-terminal sequence determination and enzymatic characterization of antiquitin from the liver of grass carp
    Wing Man Chan
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
    Comp Biochem Physiol B Biochem Mol Biol 136:443-50. 2003
    ..The optimal pH for the oxidation of acetaldehyde was 9.5. At this pH, the Vmax and the Km values for acetaldehyde were 1.95 U/mg and 2.00 mM, respectively...
  8. doi request reprint Glycosylated zinc(II) phthalocyanines as efficient photosensitisers for photodynamic therapy. Synthesis, photophysical properties and in vitro photodynamic activity
    Chi Fung Choi
    Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Org Biomol Chem 6:2173-81. 2008
    ..For the mono-glycosylated phthalocyanines, the higher uptake can be attributed to their hydrophilic saccharide units, which increase the amphiphilicity of the macrocycles...
  9. ncbi request reprint First purification of the antiquitin protein and demonstration of its enzymatic activity
    Wai Kwan Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China
    FEBS Lett 516:183-6. 2002
    ..The enzyme is highly specific towards NAD+ as the coenzyme and is totally inactive towards NADP+. Maximal enzymatic activity was found at about pH 9-10...
  10. ncbi request reprint BAM-SiPc, a novel agent for photodynamic therapy, induces apoptosis in human hepatocarcinoma HepG2 cells by a direct mitochondrial action
    Janice C Lai
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Cancer Biol Ther 5:413-8. 2006
    ....
  11. ncbi request reprint Preparation and in vitro photodynamic activity of novel silicon(IV) phthalocyanines conjugated to serum albumins
    Jian Dong Huang
    Department of Chemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    J Inorg Biochem 100:946-51. 2006
    ..As shown by absorption spectroscopy, the tetracationic phthalocyanine 3 was significantly aggregated in the protein cavity and its photocytotoxicity remained the lowest among the four photosensitizers...
  12. ncbi request reprint Synthesis and in vitro photodynamic activity of mono-substituted amphiphilic zinc(II) phthalocyanines
    Pui Chi Lo
    Department of Chemistry and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    Bioorg Med Chem Lett 17:1073-7. 2007
    ..08microM. The dicationic derivatives have a higher affinity to the cell membrane compared with the non-ionic counterparts...
  13. ncbi request reprint Highly photocytotoxic glucosylated silicon(IV) phthalocyanines. Effects of peripheral chloro substitution on the photophysical and photodynamic properties
    Pui Chi Lo
    Department of Chemistry, Department of Biochemistry, and Center of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    J Med Chem 50:2100-7. 2007
    ..Fluorescence microscopic studies have also revealed that compound 3 has a high and selective affinity to the lysosomes, but not the mitochondria, of HT29 cells...
  14. ncbi request reprint Seabream antiquitin: molecular cloning, tissue distribution, subcellular localization and functional expression
    Wai Kwan Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    FEBS Lett 579:3759-64. 2005
    ..Bacterial expression of the enzyme was also performed. The purified recombinant protein was demonstrated to exhibit similar kinetic properties as the native enzyme...
  15. ncbi request reprint Synthesis, photophysical properties and in vitro photodynamic activity of axially substituted subphthalocyanines
    Hu Xu
    Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Org Biomol Chem 5:3987-92. 2007
    ..The phenoxy analogues 4 and 5 show a relatively high photostability and are particularly potent towards these cell lines, with IC(50) values down to 0.02 microM...
  16. doi request reprint Effects of peripheral chloro substitution on the photophysical properties and in vitro photodynamic activities of galactose-conjugated silicon(IV) phthalocyanines
    Pui Chi Lo
    Department of Chemistry and Centre of Novel Functional Molecules, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    ChemMedChem 3:1110-7. 2008
    ..The nonchlorinated analogue exhibits the highest potency and can target the lysosomes of HT29 cells, whereas the monochlorinated counterpart is not localised in the lysosomes...
  17. pmc Photodynamic activity of BAM-SiPc, an unsymmetrical bisamino silicon(IV) phthalocyanine, in tumour-bearing nude mice
    S C H Leung
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    Br J Pharmacol 154:4-12. 2008
    ..The unsymmetrical bisamino silicon(IV) phthalocyanine BAM-SiPc is one of the most potent compounds. In in vitro cell culture, it exhibits high phototoxicity against a number of cancer cell lines...
  18. doi request reprint The crystal structure of seabream antiquitin reveals the structural basis of its substrate specificity
    Wai Kwan Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    FEBS Lett 582:3090-6. 2008
    ..On the other hand, Arg300 does not have any specific interaction with the alpha-carboxylate group of the substrate, but is important in maintaining the active site conformation...
  19. ncbi request reprint Pheophorbide a, an active compound isolated from Scutellaria barbata, possesses photodynamic activities by inducing apoptosis in human hepatocellular carcinoma
    Patrick Ming Kuen Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Cancer Biol Ther 5:1111-6. 2006
    ..Furthermore, in nude mice model, Pa-PDT treatment could reduce the tumor size by 57% after 14 days treatment...
  20. ncbi request reprint First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis
    Alex Y K Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Biol Chem 385:185-9. 2004
    ..Comparison of this sequence and the molecular mass with those of other protease inhibitors suggests that MCoCl belongs to the potato I inhibitor family...
  21. ncbi request reprint Pheophorbide a, an active component in Scutellaria barbata, reverses P-glycoprotein-mediated multidrug resistance on a human hepatoma cell line R-HepG2
    Patrick Ming Kuen Tang
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Cancer Biol Ther 6:504-9. 2007
    ..In addition, mechanistic study elucidated that Pa induced cell cycle arrest at G2/M phase and inhibited the expressions of G2/M phase cell cycle regulatory proteins, cyclin-A1 and cdc2 in a dose-dependent manner...
  22. ncbi request reprint J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha/beta-hydrolase fold superfamily
    Ming Hon Yau
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    FEBS Lett 580:1465-71. 2006
    ..Mutations to alanine at these positions were found to completely inactivate the enzyme. These results suggest that J1 acylase is a member of the alpha/beta-hydrolase fold superfamily with a serine-histidine-aspartate catalytic triad...
  23. ncbi request reprint Cloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region
    Chi Bun Chan
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Mol Cell Biochem 297:151-60. 2007
    ..Instead, it contains potential binding sites for cell cycle related cis-elements such as CCAAT displacement protein and cell cycle-dependent element/cell cycle genes homology region...
  24. ncbi request reprint Immunomodulatory activity of a chymotrypsin inhibitor from Momordica cochinchinensis seeds
    Alex Yuen Kam Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    J Pept Sci 12:605-11. 2006
    ..Moreover, MCoCI could also suppress the formation of hydrogen peroxide in neutrophils and macrophages. These immunomodulatory effects may explain some of the therapeutic actions of Mubiezhi...