Ym Dennis Lo

Summary

Affiliation: The Chinese University of Hong Kong
Country: China

Publications

  1. ncbi request reprint Noninvasive fetal trisomy 21 detection using chromosome-selective sequencing: a variation of the molecular counting theme
    Yuk Ming Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Expert Rev Mol Diagn 12:329-31. 2012
  2. doi request reprint Genomic analysis of fetal nucleic acids in maternal blood
    Yuk Ming Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Annu Rev Genomics Hum Genet 13:285-306. 2012
  3. pmc Placenta-derived fetal specific mRNA is more readily detectable in maternal plasma than in whole blood
    Macy M S Heung
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China
    PLoS ONE 4:e5858. 2009
  4. pmc Circulating tumour-derived nucleic acids in cancer patients: potential applications as tumour markers
    K C A Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Br J Cancer 96:681-5. 2007
  5. pmc Noninvasive prenatal diagnosis of fetal trisomy 21 by allelic ratio analysis using targeted massively parallel sequencing of maternal plasma DNA
    Gary J W Liao
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    PLoS ONE 7:e38154. 2012
  6. pmc Tracing SARS-coronavirus variant with large genomic deletion
    Rossa W K Chiu
    Emerg Infect Dis 11:168-70. 2005
  7. pmc Noninvasive prenatal diagnosis of fetal trisomy 18 and trisomy 13 by maternal plasma DNA sequencing
    Eric Z Chen
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 6:e21791. 2011
  8. pmc Epigenetic-genetic chromosome dosage approach for fetal trisomy 21 detection using an autosomal genetic reference marker
    Yu K Tong
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China
    PLoS ONE 5:e15244. 2010
  9. pmc Non-invasive prenatal assessment of trisomy 21 by multiplexed maternal plasma DNA sequencing: large scale validity study
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China
    BMJ 342:c7401. 2011
  10. ncbi request reprint Non-invasive prenatal testing using massively parallel sequencing of maternal plasma DNA: from molecular karyotyping to fetal whole-genome sequencing
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China Electronic address
    Reprod Biomed Online 27:593-8. 2013

Collaborators

Detail Information

Publications114 found, 100 shown here

  1. ncbi request reprint Noninvasive fetal trisomy 21 detection using chromosome-selective sequencing: a variation of the molecular counting theme
    Yuk Ming Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Expert Rev Mol Diagn 12:329-31. 2012
    ..This article provides a critical analysis of this approach and puts this method in the perspective of other recent works in the field...
  2. doi request reprint Genomic analysis of fetal nucleic acids in maternal blood
    Yuk Ming Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Annu Rev Genomics Hum Genet 13:285-306. 2012
    ..Understanding the ethical, legal, and social aspects in light of such rapid developments is thus a priority for future research...
  3. pmc Placenta-derived fetal specific mRNA is more readily detectable in maternal plasma than in whole blood
    Macy M S Heung
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China
    PLoS ONE 4:e5858. 2009
    ..We investigated fetal mRNA detection in maternal whole blood and determined if it offered advantages over maternal plasma analysis...
  4. pmc Circulating tumour-derived nucleic acids in cancer patients: potential applications as tumour markers
    K C A Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Br J Cancer 96:681-5. 2007
    ..In this review, different approaches for detecting tumour-associated nucleic acids in the circulation and their potential applications as tumour markers are discussed...
  5. pmc Noninvasive prenatal diagnosis of fetal trisomy 21 by allelic ratio analysis using targeted massively parallel sequencing of maternal plasma DNA
    Gary J W Liao
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    PLoS ONE 7:e38154. 2012
    ..In this study, we investigated the feasibility of analyzing single nucleotide polymorphisms using targeted massively parallel sequencing to detect such perturbations in mothers carrying trisomy 21 fetuses...
  6. pmc Tracing SARS-coronavirus variant with large genomic deletion
    Rossa W K Chiu
    Emerg Infect Dis 11:168-70. 2005
  7. pmc Noninvasive prenatal diagnosis of fetal trisomy 18 and trisomy 13 by maternal plasma DNA sequencing
    Eric Z Chen
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 6:e21791. 2011
    ..0% specificity (247 out of 252 non-trisomy 18 cases). These data indicate that with appropriate bioinformatics analysis, noninvasive prenatal diagnosis of trisomy 13 and trisomy 18 by maternal plasma DNA sequencing is achievable...
  8. pmc Epigenetic-genetic chromosome dosage approach for fetal trisomy 21 detection using an autosomal genetic reference marker
    Yu K Tong
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China
    PLoS ONE 5:e15244. 2010
    ..We explore if this method can be applied on both male and female fetuses with the use of a paternally-inherited fetal single nucleotide polymorphism (SNP) allele on a reference chromosome for chromosome dosage normalization...
  9. pmc Non-invasive prenatal assessment of trisomy 21 by multiplexed maternal plasma DNA sequencing: large scale validity study
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China
    BMJ 342:c7401. 2011
    ..To validate the clinical efficacy and practical feasibility of massively parallel maternal plasma DNA sequencing to screen for fetal trisomy 21 among high risk pregnancies clinically indicated for amniocentesis or chorionic villus sampling...
  10. ncbi request reprint Non-invasive prenatal testing using massively parallel sequencing of maternal plasma DNA: from molecular karyotyping to fetal whole-genome sequencing
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China Electronic address
    Reprod Biomed Online 27:593-8. 2013
    ..It is thus timely and important that the ethical, social and legal issues of non-invasive prenatal testing be discussed actively by all parties involved in prenatal care...
  11. pmc Non-invasive prenatal diagnosis by massively parallel sequencing of maternal plasma DNA
    Yuk Ming Dennis Lo
    Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People s Republic of China
    Open Biol 2:120086. 2012
    ..It is thus a priority that the ethical, social and legal issues regarding this technology be systematically studied...
  12. doi request reprint Fetal nucleic acids in maternal blood: the promises
    Yuk Ming Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, PR China
    Clin Chem Lab Med 50:995-8. 2012
    ..With large scale clinical validation and further reduction in costs, it is expected that the analysis of circulating fetal DNA will play an increasingly important role in the future practice of prenatal diagnosis...
  13. pmc Automated extraction protocol for quantification of SARS-coronavirus RNA in serum: an evaluation study
    Rossa W K Chiu
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 6:20. 2006
    ..In this study, we evaluated the feasibility of automating the serum RNA extraction procedure in order to increase the throughput of the assay...
  14. pmc Absence of association between angiotensin converting enzyme polymorphism and development of adult respiratory distress syndrome in patients with severe acute respiratory syndrome: a case control study
    K C Allen Chan
    The Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:26. 2005
    ..Thus, we have investigated the association between ACE insertion/deletion (I/D) polymorphism and the progression to ARDS or requirement of intensive care in SARS patients...
  15. pmc A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study
    Grace T Y Chung
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:87. 2005
    ..We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination...
  16. ncbi request reprint Recent developments in fetal nucleic acids in maternal plasma: implications to noninvasive prenatal fetal blood group genotyping
    Y M D Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
    Transfus Clin Biol 13:50-2. 2006
    ..These developments hold promise to allow the eventual widespread utilization of maternal plasma DNA analysis for the noninvasive prenatal diagnosis of blood group mismatches between the mother and fetus...
  17. doi request reprint Maternal plasma DNA sequencing reveals the genome-wide genetic and mutational profile of the fetus
    Y M Dennis Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Sci Transl Med 2:61ra91. 2010
    ..Our study suggests the feasibility of using genome-wide scanning to diagnose fetal genetic disorders prenatally in a noninvasive way...
  18. ncbi request reprint Prognostic implication of pretreatment plasma/serum concentration of Epstein-Barr virus DNA in nasopharyngeal carcinoma
    Y M Lo
    Department of Chemical Pathology and Institute of Molecular Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People s Republic of China
    Biomed Pharmacother 55:362-5. 2001
    ..In addition to NPC, it is envisaged that circulating DNA technology will find wide applications in the detection and monitoring of many other types of malignancies...
  19. ncbi request reprint Fetal DNA in maternal plasma: progress through epigenetics
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Ann N Y Acad Sci 1075:74-80. 2006
    ..These markers may catalyze the eventual clinical use of circulating fetal DNA for noninvasive prenatal diagnosis, such as for the detection of fetal chromosomal aneuploidies...
  20. ncbi request reprint Prenatal diagnosis: progress through plasma nucleic acids
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences and the Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3032 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Nat Rev Genet 8:71-7. 2007
    ..These developments have been translated into many novel genetic, epigenetic and gene-expression markers, and are expected to have a fundamental impact on the future practice of prenatal diagnosis...
  21. ncbi request reprint Plasma placental RNA allelic ratio permits noninvasive prenatal chromosomal aneuploidy detection
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Nat Med 13:218-23. 2007
    ..The allelic ratios in maternal plasma correlated with those in the placenta. Fetal trisomy 21 was detected noninvasively in 90% of cases and excluded in 96.5% of controls...
  22. pmc Digital PCR for the molecular detection of fetal chromosomal aneuploidy
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, School of Public Health, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong Special Administrative Region, People s Republic of China
    Proc Natl Acad Sci U S A 104:13116-21. 2007
    ..We applied the sequential probability ratio test to interpret the digital PCR data. Computer simulation and empirical validation confirmed the high accuracy of the disease classification algorithm...
  23. ncbi request reprint Plasma nucleic acid analysis by massively parallel sequencing: pathological insights and diagnostic implications
    Y M Lo
    Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong SAR, China
    J Pathol 225:318-23. 2011
    ..Such developments would provide new modalities for molecular diagnostics and would improve our understanding of the biology of circulating nucleic acids...
  24. ncbi request reprint Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies by maternal plasma nucleic acid analysis
    Y M Dennis Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 54:461-6. 2008
    ..The main challenge of work in this area is the fact that cell-free fetal nucleic acids represent only a minor fraction of the total nucleic acids in maternal plasma...
  25. doi request reprint Fetal nucleic acids in maternal plasma
    Y M Dennis Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Ann N Y Acad Sci 1137:140-3. 2008
    ..It is likely that these and other methods yet to be developed would allow noninvasive prenatal diagnosis of chromosomal aneuploidies by maternal plasma nucleic acids to be realized in the near future...
  26. doi request reprint Noninvasive prenatal detection of fetal chromosomal aneuploidies by maternal plasma nucleic acid analysis: a review of the current state of the art
    Y M D Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
    BJOG 116:152-7. 2009
    ..It is hoped that plasma-based molecular prenatal diagnosis might ultimately make prenatal testing safer for pregnant women and their fetuses...
  27. ncbi request reprint Noninvasive approaches to prenatal diagnosis of hemoglobinopathies using fetal DNA in maternal plasma
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Hematol Oncol Clin North Am 24:1179-86. 2010
    ..These developments have been demonstrated as feasible for noninvasive prenatal diagnosis of several hemoglobinopathies, including β-thalassemia and hemoglobin E disease...
  28. ncbi request reprint The biology and diagnostic applications of plasma RNA
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Ann N Y Acad Sci 1022:135-9. 2004
    ..This discovery has created new prenatal diagnostic and monitoring possibilities. It is expected that circulating RNA will find application in many other clinical scenarios, including emergency medicine...
  29. ncbi request reprint Fetal DNA in maternal plasma
    Y M Lo
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories
    Ann N Y Acad Sci 906:141-7. 2000
    ..It is hoped that the eagerly awaited answers to these and other questions may ultimately enhance our understanding of the fetomaternal relationship...
  30. ncbi request reprint Quantitative analysis of Epstein-Barr virus DNA in plasma and serum: applications to tumor detection and monitoring
    Y M Lo
    Department of Chemical Pathology and Institute of Molecular Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region
    Ann N Y Acad Sci 945:68-72. 2001
    ..It is expected that plasma/serum EBV DNA analysis will soon be incorporated into the routine investigative protocol of EBV-associated malignancies...
  31. ncbi request reprint The ins and outs of fetal DNA in maternal plasma
    Y M Dennis Lo
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
    Lancet 361:193-4. 2003
  32. ncbi request reprint Fetal DNA in maternal plasma: application to non-invasive blood group genotyping of the fetus
    Y M Lo
    Department of Chemical Pathology The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, SAR
    Transfus Clin Biol 8:306-10. 2001
    ..However, there is optimism that the accurate and safe prenatal determination of fetal blood group types may be achieved in routine clinical practice in the near future...
  33. ncbi request reprint Molecular prognostication of nasopharyngeal carcinoma by quantitative analysis of circulating Epstein-Barr virus DNA
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories
    Cancer Res 60:6878-81. 2000
    ..6 (95% confidence interval, 1.1-2.1 for each 10-fold increase in serum EBV DNA concentration; P = 0.007)]. The quantitation of circulating EBV DNA may thus allow improved prognostication of NPC...
  34. ncbi request reprint Circulating Epstein-Barr virus DNA in the serum of patients with gastric carcinoma
    Y M Lo
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People s Republic of China
    Clin Cancer Res 7:1856-9. 2001
    ..We investigated the detectability of EBV DNA in the serum of gastric carcinoma patients in Hong Kong. Previous data have shown that approximately 10% of gastric carcinomas in Hong Kong are associated with EBV...
  35. ncbi request reprint Quantitative analysis of the bidirectional fetomaternal transfer of nucleated cells and plasma DNA
    Y M Lo
    Departments of Chemical Pathology and Obstetrics and Gynecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 46:1301-9. 2000
    ..However, there has been no systematic quantitative comparison of these two directions and two modalities of trafficking within the same study population...
  36. ncbi request reprint Fetal DNA in maternal plasma/serum: the first 5 years
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region
    Pediatr Res 53:16-7. 2003
  37. ncbi request reprint Circulating nucleic acids in plasma and serum: an overview
    Y M Lo
    Department of Chemical Pathology and Institute of Molecular Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region
    Ann N Y Acad Sci 945:1-7. 2001
    ..Future areas of study include circulating RNA in plasma and the elucidation of the biology of release, clearance, and possible functionality of plasma nucleic acids...
  38. ncbi request reprint Fetal DNA in maternal plasma: biology and diagnostic applications
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region
    Clin Chem 46:1903-6. 2000
    ..Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis...
  39. ncbi request reprint Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, New Territories, Hong Kong Special Administrative Region
    Cancer Res 59:1188-91. 1999
    ..These results suggest that quantitative analysis of plasma EBV DNA may be a useful clinical and research tool in the screening and monitoring of NPC patients...
  40. ncbi request reprint Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories
    Cancer Res 59:3899-903. 1999
    ..Real-time quantitative MSP may have applications in elucidating diverse biological processes involving DNA methylation and may become a valuable diagnostic tool for detecting tumor-associated epigenetic changes in cancer patients...
  41. ncbi request reprint Quantitative abnormalities of fetal DNA in maternal serum in preeclampsia
    Y M Lo
    Departments of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 45:184-8. 1999
    ..To determine if quantitative abnormalities of circulating non-host DNA may be associated with certain pathologic processes, we used circulating fetal DNA in preeclampsia as a model system...
  42. ncbi request reprint Increased fetal DNA concentrations in the plasma of pregnant women carrying fetuses with trisomy 21
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 45:1747-51. 1999
    ..It is of research and potential diagnostic interest to determine whether fetal trisomy 21 may be associated with quantitative abnormalities of circulating fetal DNA in maternal plasma...
  43. ncbi request reprint Quantitative and temporal correlation between circulating cell-free Epstein-Barr virus DNA and tumor recurrence in nasopharyngeal carcinoma
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region
    Cancer Res 59:5452-5. 1999
    ..These results suggest that plasma/serum cell-free EBV DNA may be a valuable tool for the monitoring of NPC patients for the early detection of tumor recurrence...
  44. ncbi request reprint Fetal RhD genotyping from maternal plasma
    Y M Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Kong Hong Special Administration Region
    Ann Med 31:308-12. 1999
    ....
  45. ncbi request reprint Plasma DNA as a prognostic marker in trauma patients
    Y M Lo
    Department of Chemical Pathology and Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 46:319-23. 2000
    ..Recently, much interest has developed in the potential use of plasma DNA as a diagnostic and monitoring tool. We hypothesized that plasma DNA is increased in patients with trauma and may be prognostic in such patients...
  46. pmc Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis
    Y M Lo
    Department of Chemical Pathology, The University of Hong Kong, Prince Wales Hospital, Shatin, New Territories, Hong Kong
    Am J Hum Genet 62:768-75. 1998
    ..These data suggest that fetal DNA can be readily detected in maternal plasma and serum and may be a valuable source of material for noninvasive prenatal diagnosis...
  47. ncbi request reprint ACE2 gene polymorphisms do not affect outcome of severe acute respiratory syndrome
    Rossa W K Chiu
    The Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin
    Clin Chem 50:1683-6. 2004
  48. ncbi request reprint Nasopharyngeal carcinoma in situ (NPCIS)--pathologic and clinical perspectives
    Martin Wai Pak
    Division of Otorhinolaryngology, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong, SAR, China
    Head Neck 24:989-95. 2002
    ..Dysplasia or carcinoma in situ lesions (NPCIS) of the nasopharynx have rarely been reported. The prevalence, biologic behavior, and the transformation period of the pure preinvasive lesions have not been fully explained...
  49. doi request reprint Detection of circulating fetal nucleic acids: a review of methods and applications
    E C W Hung
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, and Department of Chemical Pathology, The Chinese University of Hong Kong, China
    J Clin Pathol 62:308-13. 2009
    ....
  50. ncbi request reprint Circulating nucleic acids as a tumor marker
    K C A Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region
    Histol Histopathol 17:937-43. 2002
    ..In this review, different approaches of detecting tumor-related nucleic acids in the circulation and their potential as tumor markers in the screening, monitoring and prognostication of malignant diseases are discussed...
  51. ncbi request reprint Detection of restriction enzyme-digested target DNA by PCR amplification using a stem-loop primer: application to the detection of hypomethylated fetal DNA in maternal plasma
    Yu K Tong
    Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 53:1906-14. 2007
    ..The positive detection of the cleaved hypomethylated molecules would be useful for certain targets but has not been reported...
  52. ncbi request reprint Noninvasive prenatal detection of fetal trisomy 18 by epigenetic allelic ratio analysis in maternal plasma: Theoretical and empirical considerations
    Yu K Tong
    Department of Chemical Pathology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 52:2194-202. 2006
    ..e., by analyzing the allelic ratio of a single-base variation present within DNA molecules exhibiting a placental-specific epigenetic signature in maternal plasma...
  53. ncbi request reprint Clinical applications of plasma Epstein-Barr virus DNA analysis and protocols for the quantitative analysis of the size of circulating Epstein-Barr virus DNA
    K C Allen Chan
    Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR
    Methods Mol Biol 336:111-21. 2006
    ..In this chapter, the clinical applications of plasma EBV DNA analysis and the protocols for the quantitative analysis of the size of circulating EBV DNA will be discussed...
  54. ncbi request reprint Relationship between pretreatment level of plasma Epstein-Barr virus DNA, tumor burden, and metabolic activity in advanced nasopharyngeal carcinoma
    Brigette B Y Ma
    Department of Clinical Oncology at the Sir Y K Pao Centre for Cancer, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong, China
    Int J Radiat Oncol Biol Phys 66:714-20. 2006
    ..This study tested the hypotheses that pEBV DNA reflects tumor burden and metabolic activity by evaluating its relationship with tumor volume and 18F-fluorodeoxyglucose (18F-FDG) uptake in NPC...
  55. ncbi request reprint Quantitative analysis of the transrenal excretion of circulating EBV DNA in nasopharyngeal carcinoma patients
    K C Allen Chan
    State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Clin Cancer Res 14:4809-13. 2008
    ..In this study, we used NPC as a model to investigate if circulating EBV DNA can be excreted into urine and to quantify the contribution of renal excretion to the clearance of plasma EBV DNA...
  56. ncbi request reprint Plasma Epstein-Barr virus DNA and residual disease after radiotherapy for undifferentiated nasopharyngeal carcinoma
    Anthony T C Chan
    Department of Clinical Oncology, Sir Y K Pao Centre for Cancer, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region
    J Natl Cancer Inst 94:1614-9. 2002
    ..We designed a prospective study in Hong Kong, China, to investigate the value of plasma EBV DNA as a prognostic factor for NPC...
  57. ncbi request reprint Rapid clearance of plasma Epstein-Barr virus DNA after surgical treatment of nasopharyngeal carcinoma
    Edward W H To
    Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Clin Cancer Res 9:3254-9. 2003
    ..However, this postulation would hold true only if EBV DNA is rapidly eliminated from the circulation. In this study, we determined the in vivo elimination rate of plasma EBV DNA in NPC patients...
  58. ncbi request reprint Non-invasive prenatal diagnosis: on the horizon?
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1 F Clinical Sciences Building, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong, SAR
    Pharmacogenomics 4:191-200. 2003
    ..This paper reviews the technological developments in non-invasive prenatal diagnosis...
  59. pmc Detection of the placental epigenetic signature of the maspin gene in maternal plasma
    Stephen S C Chim
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Administrative Region, China
    Proc Natl Acad Sci U S A 102:14753-8. 2005
    ..Differential DNA methylation between the placenta and maternal blood cells may be exploited to develop further markers for noninvasive prenatal assessment...
  60. ncbi request reprint Molecular diagnosis of severe acute respiratory syndrome
    Enders K O Ng
    Department of Health, Centre for Health Protection, Government of Hong Kong Special Administrative Region, Hong Kong, SAR
    Methods Mol Biol 336:163-75. 2006
    ..These quantitative systems are potentially useful for the early diagnosis of SARS and can also provide viral load information that might assist clinicians in making a prognostic evaluation of an infected individual...
  61. ncbi request reprint Recent developments in fetal DNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Ann N Y Acad Sci 1022:100-4. 2004
    ..However, it has been demonstrated that the development of fetal epigenetic markers has great potential to expand the field...
  62. ncbi request reprint Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis
    K C Allen Chan
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR
    Clin Chem 52:2211-8. 2006
    ..This methylation pattern allows the use of methylation-sensitive restriction enzyme digestion for detecting the placental-derived hypermethylated RASSF1A sequences in maternal plasma...
  63. ncbi request reprint Diagnostic and prognostic implications of circulating cell-free Epstein-Barr virus DNA in natural killer/T-cell lymphoma
    Kenny I K Lei
    Department of Clinical Oncology and The Sir Y K Pao Cancer Centre, The Chinese University of Hong Kong, Prince of Wales Hospital, The New Territories, Hong Kong Special Administrative Region, China
    Clin Cancer Res 8:29-34. 2002
    ..We investigated the potential of circulating EBV DNA as a tumor marker for this malignancy...
  64. ncbi request reprint Circulating nucleic acid analysis: diagnostic applications for acute pathologies
    R W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong, China
    Acta Neurochir Suppl 95:471-4. 2005
    ..It is expected that new diagnostic markers based on plasma RNA detection could be developed for the evaluation of acute pathologies...
  65. ncbi request reprint Mass spectrometry-based detection of hemoglobin E mutation by allele-specific base extension reaction
    Jason C H Tsang
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 53:2205-9. 2007
    ..Systematic optimization resulted in an alternative protocol, the allele-specific base extension reaction (ASBER)...
  66. ncbi request reprint Systematic search for placental DNA-methylation markers on chromosome 21: toward a maternal plasma-based epigenetic test for fetal trisomy 21
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 54:500-11. 2008
    ....
  67. ncbi request reprint Quantitative subtyping of hepatitis B virus reveals complex dynamics of YMDD motif mutants development during long-term lamivudine therapy
    Chunming Ding
    Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong
    Antivir Ther 11:1041-9. 2006
    ..Treatment of chronic hepatitis B (CHB) with lamivudine (3TC) is limited by development of drug-resistant mutants at the YMDD motif. We aimed to validate the use of mass spectrometry to detect YMDD mutants and quantify viral subpopulations...
  68. ncbi request reprint Circulating nucleic acids in plasma/serum
    Jason C H Tsang
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, SAR
    Pathology 39:197-207. 2007
    ..In conclusion, studies of circulating nucleic acids have promised exciting developments in molecular diagnostics in the years to come...
  69. doi request reprint Synergy of total PLAC4 RNA concentration and measurement of the RNA single-nucleotide polymorphism allelic ratio for the noninvasive prenatal detection of trisomy 21
    Nancy B Y Tsui
    Departments of Chemical Pathology, The Chinese University of Hong Kong, Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, New Territories, Hong Kong SAR, China
    Clin Chem 56:73-81. 2010
    ..We evaluated the diagnostic sensitivities and specificities of 2 trisomy 21-screening approaches that use maternal plasma PLAC4 mRNA...
  70. ncbi request reprint A phase II study of patients with metastatic or locoregionally recurrent nasopharyngeal carcinoma and evaluation of plasma Epstein-Barr virus DNA as a biomarker of efficacy
    Brigette Ma
    Department of Clinical Oncology and Sir Y K Pao Cancer Center, Prince of Wales Hospital, Shatin, Hong Kong
    Cancer Chemother Pharmacol 62:59-64. 2008
    ..The epidermal growth factor receptor (EGFR) is commonly overexpressed in nasopharyngeal carcinoma (NPC) and gefitinib inhibits NPC growth in vitro...
  71. ncbi request reprint The prognostic significance of tumor vascular invasion and its association with plasma Epstein-Barr virus DNA, tumor volume and metabolic activity in locoregionally advanced nasopharyngeal carcinoma
    Brigette B Y Ma
    Department of Clinical Oncology, Sir Y K Pao Centre for Cancer, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China
    Oral Oncol 44:1067-72. 2008
    ..Parapharyngeal and cavernous sinus invasion were not significant predictors of distant recurrence following chemoradiotherapy in our cohort with locoregionally advanced NPC...
  72. ncbi request reprint Application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis
    Rossa W K Chiu
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, I F Clinical Sciences Bulding, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAE, China
    Expert Rev Mol Diagn 2:32-40. 2002
    ..More recently, there have been significant new developments with expanding number of potential applications...
  73. ncbi request reprint Noninvasive prenatal exclusion of congenital adrenal hyperplasia by maternal plasma analysis: a feasibility study
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 48:778-80. 2002
  74. ncbi request reprint Noninvasive prenatal diagnosis by analysis of fetal DNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR
    Methods Mol Biol 336:101-9. 2006
    ..The protocol that has been applied regularly in the laboratory of the authors for the reliable detection and quantification of circulating fetal DNA is presented in this chapter...
  75. ncbi request reprint The biology and diagnostic applications of fetal DNA and RNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital Shatin, Hong Kong SAR, China
    Curr Top Dev Biol 61:81-111. 2004
  76. ncbi request reprint Distribution of cell-free and cell-associated Epstein-Barr virus (EBV) DNA in the blood of patients with nasopharyngeal carcinoma and EBV-associated lymphoma
    Lydia C W Lit
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 50:1842-5. 2004
  77. ncbi request reprint Phase II study of neoadjuvant carboplatin and paclitaxel followed by radiotherapy and concurrent cisplatin in patients with locoregionally advanced nasopharyngeal carcinoma: therapeutic monitoring with plasma Epstein-Barr virus DNA
    Anthony T C Chan
    Department of Clinical Oncology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong Special Administrative Region, China
    J Clin Oncol 22:3053-60. 2004
    ....
  78. ncbi request reprint Serial analysis of plasma proteomic signatures in pediatric patients with severe acute respiratory syndrome and correlation with viral load
    Terence C W Poon
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 50:1452-5. 2004
  79. ncbi request reprint The 3a protein of severe acute respiratory syndrome-associated coronavirus induces apoptosis in Vero E6 cells
    Patrick T W Law
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China
    J Gen Virol 86:1921-30. 2005
    ..In vitro experiments of chromatin condensation and DNA fragmentation suggested that the 3a protein may trigger apoptosis. These data showed that overexpression of a single SARS-CoV protein can induce apoptosis in vitro...
  80. ncbi request reprint Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases
    K F To
    Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China
    J Pathol 202:157-63. 2004
    ..Whereas the lung pathology was dominated by diffuse alveolar damage, the gut was relatively intact. These findings indicated that tissue responses to SARS-CoV infection are distinct in different organs...
  81. ncbi request reprint Improved accuracy of detection of nasopharyngeal carcinoma by combined application of circulating Epstein-Barr virus DNA and anti-Epstein-Barr viral capsid antigen IgA antibody
    Sing Fai Leung
    Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong SAR, Peoples Republic of China
    Clin Chem 50:339-45. 2004
    ....
  82. ncbi request reprint Serial analysis of the plasma concentration of SARS coronavirus RNA in pediatric patients with severe acute respiratory syndrome
    Enders K O Ng
    Department of Chemical Pathology, Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
    Clin Chem 49:2085-8. 2003
  83. ncbi request reprint Plasma nucleic acids in the diagnosis and management of malignant disease
    Philip J Johnson
    Department of Clinical Oncology, Sir YK Pao Centre for Cancer, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region
    Clin Chem 48:1186-93. 2002
    ..Recently, there has been much interest in the potential use of nucleic acid markers in plasma and serum for this purpose...
  84. pmc mRNA of placental origin is readily detectable in maternal plasma
    Enders K O Ng
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
    Proc Natl Acad Sci U S A 100:4748-53. 2003
    ..The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities...
  85. ncbi request reprint Molecular characterization of circulating EBV DNA in the plasma of nasopharyngeal carcinoma and lymphoma patients
    K C Allen Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Cancer Res 63:2028-32. 2003
    ..These results provide fundamental information that may improve our understanding of the release of tumor-derived nucleic acids into the blood of cancer patients...
  86. ncbi request reprint Diagnostic developments involving cell-free (circulating) nucleic acids
    Yu Kwan Tong
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, PR China
    Clin Chim Acta 363:187-96. 2006
    ..Regardless of the sources of cell-free DNA for clinical use, the amount is frequently scarce...
  87. ncbi request reprint Genomic sequencing of the severe acute respiratory syndrome-coronavirus
    Stephen S C Chim
    Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR
    Methods Mol Biol 336:177-94. 2006
    ..PCR amplification of the viral sequence and genomic sequencing of these critical sequence variations of re-emerging SARS-CoV strains would give us quick insights into the virus...
  88. ncbi request reprint Disparity of sensitivities in detection of radiation-naïve and postirradiation recurrent nasopharyngeal carcinoma of the undifferentiated type by quantitative analysis of circulating Epstein-Barr virus DNA1,2
    Sing Fai Leung
    Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong SAR, China
    Clin Cancer Res 9:3431-4. 2003
    ..The purpose of this research was to compare the sensitivities of plasma EBV DNA in detection of postirradiation locally recurrent nasopharyngeal carcinoma (NPC), postirradiation distant metastatic NPC, and radiation-naïve NPC...
  89. ncbi request reprint Pretherapy quantitative measurement of circulating Epstein-Barr virus DNA is predictive of posttherapy distant failure in patients with early-stage nasopharyngeal carcinoma of undifferentiated type
    Sing Fai Leung
    Department of Clinical Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
    Cancer 98:288-91. 2003
    ..This issue has management implications because different therapeutic strategies currently are employed for patients with good-risk and those with poor-risk NPC...
  90. ncbi request reprint Cell-free DNA and RNA in plasma as new tools for molecular diagnostics
    Blenda C K Wong
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories
    Expert Rev Mol Diagn 3:785-97. 2003
    ..Biologically, future work will focus on the elucidation of the origin and clearance of circulating DNA, as well as the unexpected stability of circulating RNA...
  91. doi request reprint Non-invasive prenatal diagnosis by fetal nucleic acid analysis in maternal plasma: the coming of age
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
    Semin Fetal Neonatal Med 16:88-93. 2011
    ..Accurate detection of fetal trisomy 21 by next-generation sequencing has been achieved. Many of the non-invasive prenatal tests could be introduced to the clinics as soon as cost-effective and high throughput protocols are developed...
  92. ncbi request reprint Placental RNA in maternal plasma: toward noninvasive fetal gene expression profiling
    Nancy B Y Tsui
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1 F Clinical Sciences Building, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Ann N Y Acad Sci 1075:96-102. 2006
    ..Thus, fetal mRNA measurement in maternal plasma may be a useful tool for noninvasive prenatal placental gene expressing profiling...
  93. ncbi request reprint Quantitative analysis and prognostic implication of SARS coronavirus RNA in the plasma and serum of patients with severe acute respiratory syndrome
    Enders K O Ng
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Administrative Region, China
    Clin Chem 49:1976-80. 2003
    ..We investigated whether the SARS coronavirus (SARS-CoV) can be detected in serum and plasma samples during the early stages of SARS and studied the potential prognostic implications of such an approach...
  94. ncbi request reprint Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome
    Ronald T K Pang
    Centre for Emerging Infectious Diseases, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong SAR
    Clin Chem 52:421-9. 2006
    ..Specific proteomic patterns might be present in serum in response to the infection and could be useful for early detection of the disease...
  95. ncbi request reprint Effects of early corticosteroid treatment on plasma SARS-associated Coronavirus RNA concentrations in adult patients
    Nelson Lee
    Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, China
    J Clin Virol 31:304-9. 2004
    ..The effect of corticosteroid treatment on the viral load of Severe Acute Respiratory Syndrome (SARS) patients is unknown...
  96. pmc Noninvasive prenatal diagnosis of monogenic diseases by digital size selection and relative mutation dosage on DNA in maternal plasma
    Fiona M F Lun
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology and Obstetrics and Gynaecology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Proc Natl Acad Sci U S A 105:19920-5. 2008
    ..We show that digital NASS can work in concert with digital RMD to increase the proportion of cases with classifiable fetal genotypes and to bring noninvasive prenatal diagnosis of monogenic diseases closer to reality...
  97. pmc Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Proc Natl Acad Sci U S A 105:20458-63. 2008
    ..Massively parallel plasma DNA sequencing represents a new approach that is potentially applicable to all pregnancies for the noninvasive prenatal diagnosis of fetal chromosomal aneuploidies...
  98. ncbi request reprint Recent advances in fetal nucleic acids in maternal plasma
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    J Histochem Cytochem 53:293-6. 2005
    ..It is hoped that further developments in this field will make the routine and widespread practice of noninvasive nucleic acid-based prenatal diagnosis for common pregnancy-associated disorders feasible in the near future...
  99. ncbi request reprint Detection and characterization of placental microRNAs in maternal plasma
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
    Clin Chem 54:482-90. 2008
    ..Because nucleic acids of placental origin are released into maternal plasma, we hypothesized that miRNAs produced by the placenta would also be released into maternal plasma...
  100. ncbi request reprint Circulating placental RNA in maternal plasma is associated with a preponderance of 5' mRNA fragments: implications for noninvasive prenatal diagnosis and monitoring
    Blenda C K Wong
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 51:1786-95. 2005
    ..We investigated the integrity of circulating placental RNA in maternal plasma and tested the relevance of plasma RNA integrity for noninvasive prenatal diagnosis...
  101. ncbi request reprint Clinical, virologic and immunologic profiles of a young infant with severe acute respiratory syndrome
    Frankie W T Cheng
    Department of Pediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, N T
    Pediatr Infect Dis J 24:567-8. 2005
    ..Ribavirin failed to inhibit SARS coronavirus (SARS-CoV) replication. One-step real time reverse transcription-polymerase chain reaction for plasma SARS-CoV RNA quantification was useful for early diagnosis and monitoring viremia...