R W Chiu

Summary

Affiliation: The Chinese University of Hong Kong
Country: China

Publications

  1. pmc Detection of the placental epigenetic signature of the maspin gene in maternal plasma
    Stephen S C Chim
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Administrative Region, China
    Proc Natl Acad Sci U S A 102:14753-8. 2005
  2. doi request reprint Non-invasive prenatal diagnosis by fetal nucleic acid analysis in maternal plasma: the coming of age
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
    Semin Fetal Neonatal Med 16:88-93. 2011
  3. pmc Noninvasive prenatal molecular karyotyping from maternal plasma
    Stephanie C Y Yu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    PLoS ONE 8:e60968. 2013
  4. pmc Maternal plasma fetal DNA fractions in pregnancies with low and high risks for fetal chromosomal aneuploidies
    Irena Hudecova
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    PLoS ONE 9:e88484. 2014
  5. doi request reprint Clinical applications of maternal plasma fetal DNA analysis: translating the fruits of 15 years of research
    Rossa Wai Kwun Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, P R China
    Clin Chem Lab Med 51:197-204. 2013
  6. doi request reprint Noninvasive prenatal diagnosis empowered by high-throughput sequencing
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Prenat Diagn 32:401-6. 2012
  7. pmc Absence of association between angiotensin converting enzyme polymorphism and development of adult respiratory distress syndrome in patients with severe acute respiratory syndrome: a case control study
    K C Allen Chan
    The Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:26. 2005
  8. pmc A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study
    Grace T Y Chung
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:87. 2005
  9. ncbi request reprint Circulating nucleic acid analysis: diagnostic applications for acute pathologies
    R W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong, China
    Acta Neurochir Suppl 95:471-4. 2005
  10. pmc Automated extraction protocol for quantification of SARS-coronavirus RNA in serum: an evaluation study
    Rossa W K Chiu
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 6:20. 2006

Collaborators

Detail Information

Publications80

  1. pmc Detection of the placental epigenetic signature of the maspin gene in maternal plasma
    Stephen S C Chim
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Administrative Region, China
    Proc Natl Acad Sci U S A 102:14753-8. 2005
    ..Differential DNA methylation between the placenta and maternal blood cells may be exploited to develop further markers for noninvasive prenatal assessment...
  2. doi request reprint Non-invasive prenatal diagnosis by fetal nucleic acid analysis in maternal plasma: the coming of age
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
    Semin Fetal Neonatal Med 16:88-93. 2011
    ..Accurate detection of fetal trisomy 21 by next-generation sequencing has been achieved. Many of the non-invasive prenatal tests could be introduced to the clinics as soon as cost-effective and high throughput protocols are developed...
  3. pmc Noninvasive prenatal molecular karyotyping from maternal plasma
    Stephanie C Y Yu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    PLoS ONE 8:e60968. 2013
    ..In conclusion, noninvasive prenatal molecular karyotyping from maternal plasma by massively parallel sequencing is feasible and would enhance the diagnostic spectrum of noninvasive prenatal testing...
  4. pmc Maternal plasma fetal DNA fractions in pregnancies with low and high risks for fetal chromosomal aneuploidies
    Irena Hudecova
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    PLoS ONE 9:e88484. 2014
    ..This study thus shows that the aneuploidy screening by NIPT is likely to offer similar analytical reliability without respect to the a priori fetal aneuploidy risk. ..
  5. doi request reprint Clinical applications of maternal plasma fetal DNA analysis: translating the fruits of 15 years of research
    Rossa Wai Kwun Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, P R China
    Clin Chem Lab Med 51:197-204. 2013
    ..A paradigm shift in the practice of prenatal diagnosis has begun...
  6. doi request reprint Noninvasive prenatal diagnosis empowered by high-throughput sequencing
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences and Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Prenat Diagn 32:401-6. 2012
    ..The presence of cell-free fetal DNA in maternal plasma was discovered in 1997 and offered a new noninvasive source of fetal genetic material...
  7. pmc Absence of association between angiotensin converting enzyme polymorphism and development of adult respiratory distress syndrome in patients with severe acute respiratory syndrome: a case control study
    K C Allen Chan
    The Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:26. 2005
    ..Thus, we have investigated the association between ACE insertion/deletion (I/D) polymorphism and the progression to ARDS or requirement of intensive care in SARS patients...
  8. pmc A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study
    Grace T Y Chung
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 5:87. 2005
    ..We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination...
  9. ncbi request reprint Circulating nucleic acid analysis: diagnostic applications for acute pathologies
    R W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong, China
    Acta Neurochir Suppl 95:471-4. 2005
    ..It is expected that new diagnostic markers based on plasma RNA detection could be developed for the evaluation of acute pathologies...
  10. pmc Automated extraction protocol for quantification of SARS-coronavirus RNA in serum: an evaluation study
    Rossa W K Chiu
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
    BMC Infect Dis 6:20. 2006
    ..In this study, we evaluated the feasibility of automating the serum RNA extraction procedure in order to increase the throughput of the assay...
  11. ncbi request reprint Noninvasive prenatal detection of fetal trisomy 18 by epigenetic allelic ratio analysis in maternal plasma: Theoretical and empirical considerations
    Yu K Tong
    Department of Chemical Pathology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 52:2194-202. 2006
    ..e., by analyzing the allelic ratio of a single-base variation present within DNA molecules exhibiting a placental-specific epigenetic signature in maternal plasma...
  12. ncbi request reprint Non-invasive prenatal diagnosis: on the horizon?
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1 F Clinical Sciences Building, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong, SAR
    Pharmacogenomics 4:191-200. 2003
    ..This paper reviews the technological developments in non-invasive prenatal diagnosis...
  13. ncbi request reprint Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis
    K C Allen Chan
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR
    Clin Chem 52:2211-8. 2006
    ..This methylation pattern allows the use of methylation-sensitive restriction enzyme digestion for detecting the placental-derived hypermethylated RASSF1A sequences in maternal plasma...
  14. ncbi request reprint Detection of restriction enzyme-digested target DNA by PCR amplification using a stem-loop primer: application to the detection of hypomethylated fetal DNA in maternal plasma
    Yu K Tong
    Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 53:1906-14. 2007
    ..The positive detection of the cleaved hypomethylated molecules would be useful for certain targets but has not been reported...
  15. doi request reprint Systematic search for placental DNA-methylation markers on chromosome 21: toward a maternal plasma-based epigenetic test for fetal trisomy 21
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 54:500-11. 2008
    ....
  16. ncbi request reprint Application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis
    Rossa W K Chiu
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, I F Clinical Sciences Bulding, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAE, China
    Expert Rev Mol Diagn 2:32-40. 2002
    ..More recently, there have been significant new developments with expanding number of potential applications...
  17. ncbi request reprint Recent developments in fetal DNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Ann N Y Acad Sci 1022:100-4. 2004
    ..However, it has been demonstrated that the development of fetal epigenetic markers has great potential to expand the field...
  18. doi request reprint Maternal plasma DNA sequencing reveals the genome-wide genetic and mutational profile of the fetus
    Y M Dennis Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Sci Transl Med 2:61ra91. 2010
    ..Our study suggests the feasibility of using genome-wide scanning to diagnose fetal genetic disorders prenatally in a noninvasive way...
  19. doi request reprint Noninvasive approaches to prenatal diagnosis of hemoglobinopathies using fetal DNA in maternal plasma
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Hematol Oncol Clin North Am 24:1179-86. 2010
    ..These developments have been demonstrated as feasible for noninvasive prenatal diagnosis of several hemoglobinopathies, including β-thalassemia and hemoglobin E disease...
  20. doi request reprint Synergy of total PLAC4 RNA concentration and measurement of the RNA single-nucleotide polymorphism allelic ratio for the noninvasive prenatal detection of trisomy 21
    Nancy B Y Tsui
    Departments of Chemical Pathology, The Chinese University of Hong Kong, Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, New Territories, Hong Kong SAR, China
    Clin Chem 56:73-81. 2010
    ..We evaluated the diagnostic sensitivities and specificities of 2 trisomy 21-screening approaches that use maternal plasma PLAC4 mRNA...
  21. pmc Placenta-derived fetal specific mRNA is more readily detectable in maternal plasma than in whole blood
    Macy M S Heung
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, China
    PLoS ONE 4:e5858. 2009
    ..We investigated fetal mRNA detection in maternal whole blood and determined if it offered advantages over maternal plasma analysis...
  22. ncbi request reprint Plasma placental RNA allelic ratio permits noninvasive prenatal chromosomal aneuploidy detection
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Nat Med 13:218-23. 2007
    ..The allelic ratios in maternal plasma correlated with those in the placenta. Fetal trisomy 21 was detected noninvasively in 90% of cases and excluded in 96.5% of controls...
  23. doi request reprint Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies by maternal plasma nucleic acid analysis
    Y M Dennis Lo
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 54:461-6. 2008
    ..The main challenge of work in this area is the fact that cell-free fetal nucleic acids represent only a minor fraction of the total nucleic acids in maternal plasma. Methods and..
  24. pmc Digital PCR for the molecular detection of fetal chromosomal aneuploidy
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, School of Public Health, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong Special Administrative Region, People s Republic of China
    Proc Natl Acad Sci U S A 104:13116-21. 2007
    ..We applied the sequential probability ratio test to interpret the digital PCR data. Computer simulation and empirical validation confirmed the high accuracy of the disease classification algorithm...
  25. ncbi request reprint Noninvasive prenatal diagnosis by analysis of fetal DNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR
    Methods Mol Biol 336:101-9. 2006
    ..The protocol that has been applied regularly in the laboratory of the authors for the reliable detection and quantification of circulating fetal DNA is presented in this chapter...
  26. ncbi request reprint Genomic sequencing of the severe acute respiratory syndrome-coronavirus
    Stephen S C Chim
    Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR
    Methods Mol Biol 336:177-94. 2006
    ..PCR amplification of the viral sequence and genomic sequencing of these critical sequence variations of re-emerging SARS-CoV strains would give us quick insights into the virus...
  27. ncbi request reprint Prenatal diagnosis: progress through plasma nucleic acids
    Y M Dennis Lo
    Li Ka Shing Institute of Health Sciences and the Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3032 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Nat Rev Genet 8:71-7. 2007
    ..These developments have been translated into many novel genetic, epigenetic and gene-expression markers, and are expected to have a fundamental impact on the future practice of prenatal diagnosis...
  28. ncbi request reprint The biology and diagnostic applications of fetal DNA and RNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital Shatin, Hong Kong SAR, China
    Curr Top Dev Biol 61:81-111. 2004
  29. ncbi request reprint Serial analysis of plasma proteomic signatures in pediatric patients with severe acute respiratory syndrome and correlation with viral load
    Terence C W Poon
    Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 50:1452-5. 2004
  30. pmc mRNA of placental origin is readily detectable in maternal plasma
    Enders K O Ng
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
    Proc Natl Acad Sci U S A 100:4748-53. 2003
    ..The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities...
  31. ncbi request reprint Noninvasive prenatal exclusion of congenital adrenal hyperplasia by maternal plasma analysis: a feasibility study
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 48:778-80. 2002
  32. ncbi request reprint The biology and diagnostic applications of plasma RNA
    Y M Dennis Lo
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Ann N Y Acad Sci 1022:135-9. 2004
    ..This discovery has created new prenatal diagnostic and monitoring possibilities. It is expected that circulating RNA will find application in many other clinical scenarios, including emergency medicine...
  33. ncbi request reprint Serial analysis of the plasma concentration of SARS coronavirus RNA in pediatric patients with severe acute respiratory syndrome
    Enders K O Ng
    Department of Chemical Pathology, Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
    Clin Chem 49:2085-8. 2003
  34. doi request reprint Noninvasive prenatal detection of trisomy 21 by an epigenetic-genetic chromosome-dosage approach
    Yu K Tong
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, New Territories, Hong Kong SAR, China
    Clin Chem 56:90-8. 2010
    ..The use of fetal DNA in maternal plasma for noninvasive prenatal diagnosis of trisomy 21 (T21) is an actively researched area. We propose a novel method of T21 detection that combines fetal-specific epigenetic and genetic markers...
  35. doi request reprint Non-invasive prenatal detection of fetal trisomy 18 by RNA-SNP allelic ratio analysis using maternal plasma SERPINB2 mRNA: a feasibility study
    Nancy B Y Tsui
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Prenat Diagn 29:1031-7. 2009
    ..We investigated the feasibility of applying this approach for the non-invasive prenatal detection of fetal trisomy 18...
  36. doi request reprint Detection and characterization of placental microRNAs in maternal plasma
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
    Clin Chem 54:482-90. 2008
    ..Because nucleic acids of placental origin are released into maternal plasma, we hypothesized that miRNAs produced by the placenta would also be released into maternal plasma...
  37. ncbi request reprint Evaluation of human chorionic gonadotropin beta-subunit mRNA concentrations in maternal serum in aneuploid pregnancies: a feasibility study
    Enders K O Ng
    Departments of Chemical Pathology and Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
    Clin Chem 50:1055-7. 2004
  38. doi request reprint Microfluidics digital PCR reveals a higher than expected fraction of fetal DNA in maternal plasma
    Fiona M F Lun
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
    Clin Chem 54:1664-72. 2008
    ..We tested the hypothesis that microfluidics digital PCR, in which individual fetal-DNA molecules are counted, could enhance the precision of measuring circulating fetal DNA...
  39. ncbi request reprint Quantitative analysis and prognostic implication of SARS coronavirus RNA in the plasma and serum of patients with severe acute respiratory syndrome
    Enders K O Ng
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Administrative Region, China
    Clin Chem 49:1976-80. 2003
    ..We investigated whether the SARS coronavirus (SARS-CoV) can be detected in serum and plasma samples during the early stages of SARS and studied the potential prognostic implications of such an approach...
  40. pmc Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
    Proc Natl Acad Sci U S A 105:20458-63. 2008
    ..Massively parallel plasma DNA sequencing represents a new approach that is potentially applicable to all pregnancies for the noninvasive prenatal diagnosis of fetal chromosomal aneuploidies...
  41. pmc Noninvasive prenatal diagnosis of monogenic diseases by digital size selection and relative mutation dosage on DNA in maternal plasma
    Fiona M F Lun
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology and Obstetrics and Gynaecology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Proc Natl Acad Sci U S A 105:19920-5. 2008
    ..We show that digital NASS can work in concert with digital RMD to increase the proportion of cases with classifiable fetal genotypes and to bring noninvasive prenatal diagnosis of monogenic diseases closer to reality...
  42. ncbi request reprint Circulating placental RNA in maternal plasma is associated with a preponderance of 5' mRNA fragments: implications for noninvasive prenatal diagnosis and monitoring
    Blenda C K Wong
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 51:1786-95. 2005
    ..We investigated the integrity of circulating placental RNA in maternal plasma and tested the relevance of plasma RNA integrity for noninvasive prenatal diagnosis...
  43. ncbi request reprint Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome
    Ronald T K Pang
    Centre for Emerging Infectious Diseases, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong SAR
    Clin Chem 52:421-9. 2006
    ..Specific proteomic patterns might be present in serum in response to the infection and could be useful for early detection of the disease...
  44. ncbi request reprint Effects of early corticosteroid treatment on plasma SARS-associated Coronavirus RNA concentrations in adult patients
    Nelson Lee
    Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, China
    J Clin Virol 31:304-9. 2004
    ..The effect of corticosteroid treatment on the viral load of Severe Acute Respiratory Syndrome (SARS) patients is unknown...
  45. ncbi request reprint Clinical, virologic and immunologic profiles of a young infant with severe acute respiratory syndrome
    Frankie W T Cheng
    Department of Pediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, N T
    Pediatr Infect Dis J 24:567-8. 2005
    ..Ribavirin failed to inhibit SARS coronavirus (SARS-CoV) replication. One-step real time reverse transcription-polymerase chain reaction for plasma SARS-CoV RNA quantification was useful for early diagnosis and monitoring viremia...
  46. ncbi request reprint Quantitative aberrations of hypermethylated RASSF1A gene sequences in maternal plasma in pre-eclampsia
    Dana W Y Tsui
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR
    Prenat Diagn 27:1212-8. 2007
    ..To study if quantitative aberrations in circulating placental-derived hypermethylated RASSF1A DNA in maternal plasma are associated with pre-eclamptic pregnancies...
  47. ncbi request reprint Lack of dramatic enrichment of fetal DNA in maternal plasma by formaldehyde treatment
    Grace T Y Chung
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China
    Clin Chem 51:655-8. 2005
  48. pmc Hypermethylation of RASSF1A in human and rhesus placentas
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, China
    Am J Pathol 170:941-50. 2007
    ..These observations extend the analogy between the primate placenta and malignant tumors to the epigenetic level...
  49. doi request reprint Maternal plasma DNA analysis with massively parallel sequencing by ligation for noninvasive prenatal diagnosis of trisomy 21
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Hong Kong SAR, China
    Clin Chem 56:459-63. 2010
    ..The quantification of several other human chromosomes, including chromosomes 18 and 13, has been shown to be less precise, however, with quantitative biases related to the chromosomal GC content...
  50. doi request reprint A strategy for identifying circulating placental RNA markers for fetal growth assessment
    Winnie W I Pang
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, China
    Prenat Diagn 29:495-504. 2009
    ..To evaluate whether circulating placental mRNAs in maternal plasma could serve as markers for the assessment of fetal growth or intrauterine growth restriction (IUGR)...
  51. ncbi request reprint Detection of trisomy 21 by quantitative mass spectrometric analysis of single-nucleotide polymorphisms
    Nancy B Y Tsui
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Special Administrative Region, China
    Clin Chem 51:2358-62. 2005
  52. doi request reprint Non-invasive prenatal diagnosis by single molecule counting technologies
    Rossa W K Chiu
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, China
    Trends Genet 25:324-31. 2009
    ..The analytical power of these methods, namely sensitivity, specificity, accuracy and precision, should catalyse the eventual clinical use of non-invasive prenatal diagnosis...
  53. ncbi request reprint Mass spectrometry-based detection of hemoglobin E mutation by allele-specific base extension reaction
    Jason C H Tsang
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
    Clin Chem 53:2205-9. 2007
    ..Systematic optimization resulted in an alternative protocol, the allele-specific base extension reaction (ASBER)...
  54. ncbi request reprint Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation
    Yanni Y N Lui
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 48:421-7. 2002
    ..We used a sex-mismatched bone marrow transplantation model to study the relative contribution of hematopoietic and nonhematopoietic cells to circulating DNA...
  55. ncbi request reprint Prenatal exclusion of beta thalassaemia major by examination of maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1 F Clinical Sciences Building, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR, Hong Kong
    Lancet 360:998-1000. 2002
    ..The fetal genotype was completely concordant with conventional analysis and beta thalassaemia major was excluded in two of the pregnancies non-invasively...
  56. doi request reprint Aberrant concentrations of liver-derived plasma albumin mRNA in liver pathologies
    Rebecca W Y Chan
    Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, New Territories, Hong Kong SAR, China
    Clin Chem 56:82-9. 2010
    ..We hypothesized that liver-derived mRNA, such as ALB (albumin) mRNA, would be released into human plasma with liver cell death...
  57. ncbi request reprint Cell-free nucleic acids in plasma, serum and urine: a new tool in molecular diagnosis
    Allen K C Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30 32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR
    Ann Clin Biochem 40:122-30. 2003
    ..Some of the findings are on the verge of being translated into clinical use. DNA is also now being sought in other body fluids such as urine...
  58. ncbi request reprint Time profile of appearance and disappearance of circulating placenta-derived mRNA in maternal plasma
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
    Clin Chem 52:313-6. 2006
    ..Fetal RNA of placental origin has been detected in the plasma of pregnant women, but the timing of the first appearance and the detailed kinetics of postdelivery clearance of such circulating RNA have not been studied...
  59. ncbi request reprint Plasma mitochondrial DNA concentrations after trauma
    Nicole Y L Lam
    Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 50:213-6. 2004
  60. doi request reprint Presence of donor-derived DNA and cells in the urine of sex-mismatched hematopoietic stem cell transplant recipients: implication for the transrenal hypothesis
    Emily C W Hung
    Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Clin Chem 55:715-22. 2009
    ..Although DNA in the urine has the potential to provide a completely noninvasive source of nucleic acids for molecular diagnosis, its existence remains controversial...
  61. ncbi request reprint Effects of filtration on glyceraldehyde-3-phosphate dehydrogenase mRNA in the plasma of trauma patients and healthy individuals
    Timothy H Rainer
    Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 50:206-8. 2004
  62. ncbi request reprint Genomic sequencing of a SARS coronavirus isolate that predated the Metropole Hotel case cluster in Hong Kong
    Stephen S C Chim
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1 F Clinical Sciences Bldg, 30 32 Ngan Shing St, Shatin, New Territories, Hong Kong SAR
    Clin Chem 50:231-3. 2004
  63. ncbi request reprint The concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsia
    Enders K O Ng
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 49:727-31. 2003
    ..Increased fetal DNA in maternal plasma/serum has been reported in pregnancies complicated by preeclampsia. We hypothesize that fetal RNA may also be increased in maternal plasma in preeclampsia...
  64. ncbi request reprint Quantitative analysis of circulating mitochondrial DNA in plasma
    Rossa W K Chiu
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
    Clin Chem 49:719-26. 2003
    ..The aim of this study was to develop an assay to quantify mitochondrial DNA in the plasma of healthy individuals...
  65. pmc MS analysis of single-nucleotide differences in circulating nucleic acids: Application to noninvasive prenatal diagnosis
    Chunming Ding
    Bioinformatics Program and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 101:10762-7. 2004
    ..This analytical approach also will have implications for many other applications of circulating nucleic acids in areas such as oncology and transplantation...
  66. pmc Tracing SARS-coronavirus variant with large genomic deletion
    Rossa W K Chiu
    Emerg Infect Dis 11:168-70. 2005
  67. ncbi request reprint Detection of SARS coronavirus RNA in the cerebrospinal fluid of a patient with severe acute respiratory syndrome
    Emily C W Hung
    Clin Chem 49:2108-9. 2003
  68. ncbi request reprint ACE2 gene polymorphisms do not affect outcome of severe acute respiratory syndrome
    Rossa W K Chiu
    The Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin
    Clin Chem 50:1683-6. 2004
  69. ncbi request reprint Molecular epidemiology of SARS--from Amoy Gardens to Taiwan
    Rossa W K Chiu
    N Engl J Med 349:1875-6. 2003
  70. ncbi request reprint Free fetal DNA in maternal circulation
    Y M Dennis Lo
    JAMA 292:2835; author reply 2835-6. 2004
  71. ncbi request reprint Serum amyloid A is not useful in the diagnosis of severe acute respiratory syndrome
    Ronald T K Pang
    Clin Chem 52:1202-4. 2006
  72. ncbi request reprint EDTA is a better anticoagulant than heparin or citrate for delayed blood processing for plasma DNA analysis
    Nicole Y L Lam
    Clin Chem 50:256-7. 2004
  73. ncbi request reprint Mass spectrometric detection of an SNP panel as an internal positive control for fetal DNA analysis in maternal plasma
    Katherine C K Chow
    Clin Chem 53:141-2. 2007
  74. ncbi request reprint Non-invasive prenatal diagnosis of Down's syndrome
    Y M Dennis Lo
    Lancet 369:1997; author reply 1998-9. 2007
  75. ncbi request reprint Epigenetic analysis of RASSF1A gene in cell-free DNA in amniotic fluid
    Fiona M F Lun
    Clin Chem 53:796-8. 2007
  76. ncbi request reprint Presence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individuals
    Enders K O Ng
    Institute of Molecular Oncology and Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region
    Clin Chem 48:1212-7. 2002
    ..As RNA is labile, we investigated whether circulating RNA in human plasma may be present in a particle-associated form...
  77. ncbi request reprint Comparison of protocols for extracting circulating DNA and RNA from maternal plasma
    Rossa W K Chiu
    Clin Chem 51:2209-10. 2005
  78. ncbi request reprint Fetal rhesus D mRNA is not detectable in maternal plasma
    Rossa W K Chiu
    Clin Chem 51:2210-1. 2005
  79. ncbi request reprint Detrimental effect of formaldehyde on plasma RNA detection
    Grace T Y Chung
    Clin Chem 51:1074-6. 2005
  80. ncbi request reprint Plasma beta-globin DNA as a prognostic marker in chest pain patients
    Timothy H Rainer
    Accident and Emergency Medicine Academic Unit, Hong Kong SAR
    Clin Chim Acta 368:110-3. 2006
    ..Acute coronary syndrome may involve cell death and the release of nucleic acids into the circulation. We thus investigated whether plasma DNA concentrations are increased and determined its prognostic significance in patients with ACS...