Sheng Chen

Summary

Affiliation: The Hong Kong Polytechnic University
Country: China

Publications

  1. doi request reprint Solubility of the catalytic domains of Botulinum neurotoxin serotype E subtypes
    Sheng Chen
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region Electronic address
    Protein Expr Purif 118:18-24. 2016
  2. doi request reprint Ebselen as a potent covalent inhibitor of New Delhi metallo-β-lactamase (NDM-1)
    Jiachi Chiou
    Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong Polytechnic University, Shenzhen Research Institute, Shenzhen, P R China
    Chem Commun (Camb) 51:9543-6. 2015
  3. pmc Characterization of a novel zinc transporter ZnuA acquired by Vibrio parahaemolyticus through horizontal gene transfer
    Ming Liu
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Hong Kong, China Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute Shenzhen, China
    Front Cell Infect Microbiol 3:61. 2013
  4. pmc Clinical uses of botulinum neurotoxins: current indications, limitations and future developments
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Toxins (Basel) 4:913-39. 2012
  5. doi request reprint Engineering Clostridia Neurotoxins with elevated catalytic activity
    Jiubiao Guo
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region
    Toxicon 74:158-66. 2013
  6. pmc Unique substrate recognition mechanism of the botulinum neurotoxin D light chain
    Jiubiao Guo
    From the Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
    J Biol Chem 288:27881-7. 2013
  7. pmc Insights into the different catalytic activities of Clostridium neurotoxins
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR
    Biochemistry 51:3941-7. 2012
  8. doi request reprint IncI1 Plasmids Carrying Various blaCTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China
    Marcus Ho Yin Wong
    State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China
    Antimicrob Agents Chemother 60:982-9. 2015
  9. doi request reprint Residues Distal to the Active Site Contribute to Enhanced Catalytic Activity of Variant and Hybrid β-Lactamases Derived from CTX-M-14 and CTX-M-15
    Dandan He
    College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues SCAU, South China Agricultural University, Guangzhou, People s Republic of China Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University, Shen Zhen Research Institute, Shenzhen, People s Republic of China State Key Laboratory of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR
    Antimicrob Agents Chemother 59:5976-83. 2015
  10. doi request reprint Structure-based design, synthesis, and biological evaluation of isatin derivatives as potential glycosyltransferase inhibitors
    Yong Wang
    Department of Applied Biology and Chemical Technology and State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
    Chem Biol Drug Des 84:685-96. 2014

Collaborators

  • Xuechen Li
  • Yanxiang Zhao
  • Jiubiao Guo
  • Marcus Ho Yin Wong
  • Edward Wai Chi Chan
  • Jiachi Chiou
  • Ming Liu
  • Ruichao Li
  • Dachuan Lin
  • Meiying Yan
  • Dandan He
  • Kathy Hiu Laam Po
  • Edward Waichi Chan
  • Jinglin Wang
  • Kaichao Chen
  • Pui Kin So
  • Kin Fai Chan
  • Kwok Yin Wong
  • Lizhang Liu
  • Yong Wang
  • Yuanjie Zhou
  • Shan Gao
  • Tak Hang Chan
  • Jian Hua Liu
  • Lanping Liu
  • Li Zeng
  • Jiang Tao
  • Bin Ji
  • Zhenling Zeng
  • Xiaojie Chen
  • Biao Kan
  • Shengbiao Wan
  • Edward Wai-Chi Chan
  • Siu Cheong Yan
  • Ning Sun
  • Hok Kiu Lui
  • Kan Biao
  • Ci Xu
  • Yun Chung Leung
  • Song Lin
  • Thomas Yun Chung Leung
  • Fung Yi Chan
  • Ruben Abagyan
  • Xuehua Pan

Detail Information

Publications30

  1. doi request reprint Solubility of the catalytic domains of Botulinum neurotoxin serotype E subtypes
    Sheng Chen
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region Electronic address
    Protein Expr Purif 118:18-24. 2016
    ..In conclusion, this study generated a soluble and stable recombinant LC/E and provided insight into the structural components that govern the solubility and stability of the LCs of other BoNT serotypes and Tetanus toxin. ..
  2. doi request reprint Ebselen as a potent covalent inhibitor of New Delhi metallo-β-lactamase (NDM-1)
    Jiachi Chiou
    Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong Polytechnic University, Shenzhen Research Institute, Shenzhen, P R China
    Chem Commun (Camb) 51:9543-6. 2015
    ....
  3. pmc Characterization of a novel zinc transporter ZnuA acquired by Vibrio parahaemolyticus through horizontal gene transfer
    Ming Liu
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Hong Kong, China Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute Shenzhen, China
    Front Cell Infect Microbiol 3:61. 2013
    ..These results suggest that the horizontally acquired znuA subfamily gene, vpa1307, contributes to the fitness and virulence of Vibrio species. ..
  4. pmc Clinical uses of botulinum neurotoxins: current indications, limitations and future developments
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Toxins (Basel) 4:913-39. 2012
    ..Much research still needs to be done to improve and extend the medical uses of BoNTs...
  5. doi request reprint Engineering Clostridia Neurotoxins with elevated catalytic activity
    Jiubiao Guo
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region
    Toxicon 74:158-66. 2013
    ..The LC/B derivative with elevated activity has the potential to be developed into novel therapy to minimize the impact of immunoresistance during BoNT/B therapy. ..
  6. pmc Unique substrate recognition mechanism of the botulinum neurotoxin D light chain
    Jiubiao Guo
    From the Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
    J Biol Chem 288:27881-7. 2013
    ..In conclusion, in this study, we identified a unique multistep substrate recognition mechanism by LC/D and provide insights for LC/D engineering and antitoxin development. ..
  7. pmc Insights into the different catalytic activities of Clostridium neurotoxins
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR
    Biochemistry 51:3941-7. 2012
    ..This analysis explains the molecular basis underlying the VAMP2 recognition and cleavage by LC/B and LC/T and provides insight that may extend the pharmacologic utility of these neurological reagents...
  8. doi request reprint IncI1 Plasmids Carrying Various blaCTX-M Genes Contribute to Ceftriaxone Resistance in Salmonella enterica Serovar Enteritidis in China
    Marcus Ho Yin Wong
    State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China
    Antimicrob Agents Chemother 60:982-9. 2015
    ....
  9. doi request reprint Residues Distal to the Active Site Contribute to Enhanced Catalytic Activity of Variant and Hybrid β-Lactamases Derived from CTX-M-14 and CTX-M-15
    Dandan He
    College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues SCAU, South China Agricultural University, Guangzhou, People s Republic of China Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University, Shen Zhen Research Institute, Shenzhen, People s Republic of China State Key Laboratory of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR
    Antimicrob Agents Chemother 59:5976-83. 2015
    ..These data indicate that residues distal to the active site of CTX-Ms contributed to their enhanced catalytic activities to ESCs. ..
  10. doi request reprint Structure-based design, synthesis, and biological evaluation of isatin derivatives as potential glycosyltransferase inhibitors
    Yong Wang
    Department of Applied Biology and Chemical Technology and State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
    Chem Biol Drug Des 84:685-96. 2014
    ..Molecular docking study suggests a potential binding pocket of 4l in the GT domain of S. aureus PBP2. Taken together, compound 4l would provide a new scaffold for further development of potent GT inhibitors...
  11. doi request reprint Dissemination of IncI2 Plasmids That Harbor the blaCTX-M Element among Clinical Salmonella Isolates
    Marcus Ho Yin Wong
    Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Antimicrob Agents Chemother 59:5026-8. 2015
    ..coli strains recovered from animals. This finding indicates that IncI2 plasmids harboring the blaCTX-M genes may undergo cross-species migration among potential bacterial pathogens, with E. coli as the major source of such elements. ..
  12. doi request reprint Increasing prevalence of ciprofloxacin-resistant food-borne Salmonella strains harboring multiple PMQR elements but not target gene mutations
    Dachuan Lin
    Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, P R China
    Sci Rep 5:14754. 2015
    ..To summarize, findings in this work suggested that PMQRs greatly facilitate development of fluoroquinolone resistance in Salmonella by abolishing the requirement of target gene mutations. ..
  13. doi request reprint First detection of AmpC β-lactamase bla(CMY-2) on a conjugative IncA/C plasmid in a Vibrio parahaemolyticus isolate of food origin
    Ruichao Li
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Antimicrob Agents Chemother 59:4106-11. 2015
    ..parahaemolyticus, thereby posing a potential threat to public health...
  14. doi request reprint CARB-17 family of β-lactamases mediates intrinsic resistance to penicillins in Vibrio parahaemolyticus
    Jiachi Chiou
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People s Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Antimicrob Agents Chemother 59:3593-5. 2015
    ..Importantly, bla(CARB-17)-like genes were present in all 293 V. parahaemolyticus genome sequences available in GenBank and detectable in all 91 V. parahaemolyticus food isolates, further confirming the intrinsic nature of this gene...
  15. doi request reprint Complete nucleotide sequence of a conjugative plasmid carrying bla(PER-1)
    Ruichao Li
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People s Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Antimicrob Agents Chemother 59:3582-4. 2015
    ..A complex class 1 integron was identified together with ISCR1 and bla(PER-1) (ISCR1-bla(PER-1)-gst-abct-qacEΔ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination of bla(PER-1)...
  16. doi request reprint Substrate-based inhibitors exhibiting excellent protective and therapeutic effects against Botulinum Neurotoxin A intoxication
    Jiubiao Guo
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P R China
    Sci Rep 5:16981. 2015
    ....
  17. doi request reprint Comparative characterization of botulinum neurotoxin subtypes F1 and F7 featuring differential substrate recognition and cleavage mechanisms
    Jiubiao Guo
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Toxicon 111:77-85. 2016
    ..Our findings provide insights into the distinct substrate recognition features of BoNT subtypes and useful information for therapy development for BoNT/F. ..
  18. pmc Emergence of clinical Salmonella enterica serovar Typhimurium isolates with concurrent resistance to ciprofloxacin, ceftriaxone, and azithromycin
    Marcus Ho Yin Wong
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China
    Antimicrob Agents Chemother 58:3752-6. 2014
    ..The dissemination of resistance genes, such as those encoding ESBLs and the OqxAB pump, among Salmonella organisms will speed up the selection of XDR Salmonella, posing a huge threat to public health and Salmonella infection control...
  19. doi request reprint Mechanism of substrate recognition by the novel Botulinum Neurotoxin subtype F5
    Jiubiao Guo
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P R China
    Sci Rep 6:19875. 2016
    ..The study provides direct evidence of the evolutionary adaption of BoNT to recognize its substrate which is useful for effective antitoxin and inhibitor development. ..
  20. doi request reprint Evolution and dissemination of OqxAB-like efflux pumps, an emerging quinolone resistance determinant among members of Enterobacteriaceae
    Marcus Ho Yin Wong
    Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Antimicrob Agents Chemother 59:3290-7. 2015
    ....
  21. doi request reprint Mutational Analysis of Quinolone Resistance Protein QnrVC7 Provides Novel Insights into the Structure-Activity Relationship of Qnr Proteins
    Kathy Hiu Laam Po
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, People s Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People s Republic of China
    Antimicrob Agents Chemother 60:1939-42. 2016
    ..Some substitutions reduced the protective activity of QnrVC7, while some of them increased it. These findings advanced our understanding on the detailed structural organization and functional requirements of Qnr proteins. ..
  22. doi request reprint Exploration of endogenous substrate cleavage by various forms of botulinum neurotoxins
    Jiubiao Guo
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PR China
    Toxicon 100:42-5. 2015
    ....
  23. doi request reprint A novel adhesive factor contributing to the virulence of Vibrio parahaemolyticus
    Ming Liu
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China
    Sci Rep 5:14449. 2015
    ..Our finding suggested that VpadF is a major virulence factor of V. parahaemolyticus and a potential good candidate for V. parahaemolyticus infection control for both vaccine development and drug target. ..
  24. pmc Molecular mechanisms of substrate recognition and specificity of New Delhi metallo-β-lactamase
    Jiachi Chiou
    State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, People s Republic of China
    Antimicrob Agents Chemother 58:5372-8. 2014
    ..This study provides insights into the development of efficient inhibitors for NDM-1 and offers an efficient tactic with which to study the substrate specificities of other β-lactamases...
  25. pmc Association of botulinum neurotoxin serotype A light chain with plasma membrane-bound SNAP-25
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom Kowloon, Hong Kong
    J Biol Chem 286:15067-72. 2011
    ..These findings explain how LC/A associates with SNAP-25 on the plasma membrane and provide a basis for LC/A cleavage of SNAP-25 within the SNARE complex...
  26. doi request reprint Expression and biochemical characterization of light chains of Botulinum neurotoxin subtypes F5 and F7
    Jiubiao Guo
    Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Protein Expr Purif 111:87-90. 2015
    ....
  27. pmc A simple, rapid and sensitive FRET assay for botulinum neurotoxin serotype B detection
    Jiubiao Guo
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR
    PLoS ONE 9:e114124. 2014
    ..Importantly, FVP-B showed the potential to be scaled up and used in high throughput screening of LC/B inhibitor. The currently developed FRET assay is one of the most economic and rapid FRET assays for LC/B detection. ..
  28. doi request reprint Molecular characterisation of a multidrug resistance conjugative plasmid from Vibrio parahaemolyticus
    Ming Liu
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China
    Int J Antimicrob Agents 42:575-9. 2013
    ..Transmission of this multidrug resistance conjugative plasmid among Vibrio spp. would compromise the effectiveness of Vibrio infection control and pose a huge threat to public health...
  29. doi request reprint Increasing prevalence of hydrogen sulfide negative Salmonella in retail meats
    Dachuan Lin
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, PR China
    Food Microbiol 43:1-4. 2014
    ..Such finding warrants the development of an improved method for effective coverage of H2S negative Salmonella. ..