Yuan Sun

Summary

Country: China

Publications

  1. pmc Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus
    Yuan Sun
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Virol J 8:452. 2011
  2. doi request reprint Prime-boost immunization using alphavirus replicon and adenovirus vectored vaccines induces enhanced immune responses against classical swine fever virus in mice
    He ping Zhao
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    Vet Immunol Immunopathol 131:158-66. 2009
  3. doi request reprint Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Res Vet Sci 88:77-82. 2010
  4. doi request reprint Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Vet Immunol Immunopathol 137:20-7. 2010
  5. ncbi request reprint [Generation and immunogenicity of a recombinant adenovirus expressing the E2 protein of classical swine fever virus in rabbits]
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, China
    Sheng Wu Gong Cheng Xue Bao 24:1734-9. 2008
  6. doi request reprint Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice
    Miao Li
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001 Harbin, Heilongjiang, China
    Immunol Lett 125:145-50. 2009
  7. doi request reprint Assessment of the cell-mediated immunity induced by alphavirus replicon-vectored DNA vaccines against classical swine fever in a mouse model
    He ping Zhao
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    Vet Immunol Immunopathol 129:57-65. 2009
  8. doi request reprint Development of a loop-mediated isothermal amplification for visual detection of the HCLV vaccine against classical swine fever in China
    Xing Juan Zhang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol Methods 171:200-5. 2011
  9. doi request reprint A novel alphavirus replicon-vectored vaccine delivered by adenovirus induces sterile immunity against classical swine fever
    Yuan Sun
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    Vaccine 29:8364-72. 2011
  10. pmc Hemoglobin subunit beta interacts with the capsid protein and antagonizes the growth of classical swine fever virus
    Dan Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol 87:5707-17. 2013

Collaborators

Detail Information

Publications37

  1. pmc Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus
    Yuan Sun
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Virol J 8:452. 2011
    ..Currently, larger domestic pigs are only animals widely used in vaccine evaluation and pathogenicity study of classical swine fever virus (CSFV). This study was aimed to create an alternative animal experimental infection model of CSFV...
  2. doi request reprint Prime-boost immunization using alphavirus replicon and adenovirus vectored vaccines induces enhanced immune responses against classical swine fever virus in mice
    He ping Zhao
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    Vet Immunol Immunopathol 131:158-66. 2009
    ..These results support the notion that a regimen of DNA prime-recombinant adenovirus boost enhances humoral and cell-mediated immune responses, and the DNA prime-protein boost regimen enhances humoral immune responses...
  3. doi request reprint Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Res Vet Sci 88:77-82. 2010
    ..This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF...
  4. doi request reprint Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Vet Immunol Immunopathol 137:20-7. 2010
    ..The results demonstrate that the heterologous DNA prime and recombinant adenovirus boost strategy can induce solid protective immunity...
  5. ncbi request reprint [Generation and immunogenicity of a recombinant adenovirus expressing the E2 protein of classical swine fever virus in rabbits]
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, China
    Sheng Wu Gong Cheng Xue Bao 24:1734-9. 2008
    ..This indicates that the recombinant adenovirus can be an attractive candidate vaccine against CSF...
  6. doi request reprint Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice
    Miao Li
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001 Harbin, Heilongjiang, China
    Immunol Lett 125:145-50. 2009
    ..This is a first step in the demonstration that the pseudotyped baculovirus-delivered CSFV E2 gene can be a potential non-replicating vaccine against CSFV infections...
  7. doi request reprint Assessment of the cell-mediated immunity induced by alphavirus replicon-vectored DNA vaccines against classical swine fever in a mouse model
    He ping Zhao
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    Vet Immunol Immunopathol 129:57-65. 2009
    ..These findings suggest that the alphavirus replicon-delivered DNA vaccines are capable of inducing CMI, and PRV VP22 is able to enhance the immunogenicity of the co-delivered antigen...
  8. doi request reprint Development of a loop-mediated isothermal amplification for visual detection of the HCLV vaccine against classical swine fever in China
    Xing Juan Zhang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol Methods 171:200-5. 2011
    ..4% for the detection of 72 batches of HCLV vaccine. The assay provides a rapid tool for the control of vaccine quality and can be an accompanying assay of the LAMP for wild-type CSFV described previously for differential diagnosis...
  9. doi request reprint A novel alphavirus replicon-vectored vaccine delivered by adenovirus induces sterile immunity against classical swine fever
    Yuan Sun
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    Vaccine 29:8364-72. 2011
    ..The new-concept vaccination strategy may also be valuable in vaccine development against other pathogens...
  10. pmc Hemoglobin subunit beta interacts with the capsid protein and antagonizes the growth of classical swine fever virus
    Dan Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol 87:5707-17. 2013
    ..This study reports for the first time the novel role of HB in innate immunity...
  11. doi request reprint Comprehensive evaluation of the adenovirus/alphavirus-replicon chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever
    Yuan Sun
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Vaccine 31:538-44. 2013
    ..Taken together, the chimeric vaccine represents a promising marker vaccine candidate for control and eradication of CSF...
  12. doi request reprint Comparison of the protective efficacy of recombinant adenoviruses against classical swine fever
    Yuan Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Immunol Lett 135:43-9. 2011
    ..Data from this study could assist in making decisions for further development of recombinant adenoviruses as vaccine candidates against CSF...
  13. ncbi request reprint [Immune responses induced by the suicidal DNA vaccines co-expressing the GP5 protein of PRRSV and the E2 protein of CSFV in mice]
    Jianfu Sun
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy ofAgricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 24:1714-22. 2008
    ..Taken together, the suicidal DNA vaccines co-expressing GP5 and E2 could induce PRRSV and CSFV specific humoral and cell-mediated immune responses...
  14. ncbi request reprint [A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever]
    Yuan Sun
    Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 25:679-85. 2009
    ..This demonstrates that the heterologous prime-boost vaccination regime has the potential to prevent against virulent challenge...
  15. doi request reprint Simultaneous detection of Classical swine fever virus and North American genotype Porcine reproductive and respiratory syndrome virus using a duplex real-time RT-PCR
    Dan Cheng
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    J Virol Methods 151:194-9. 2008
    ..These results were 99.4% in agreement with those using conventional RT-PCRs. Therefore, the assay provides sensitive and simultaneous detection and differentiation of CSFV and PRRSV...
  16. pmc Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus
    Yongfeng Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Clin Microbiol 51:2710-2. 2013
    ..The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation. ..
  17. doi request reprint Enhancement of the immunogenicity of an alphavirus replicon-based DNA vaccine against classical swine fever by electroporation and coinjection with a plasmid expressing porcine interleukin 2
    Da Yong Tian
    Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Vaccine 30:3587-94. 2012
    ..We conclude that EP in combination with IL-2 can significantly improve the immunogenicity of the plasmid DNA vaccine...
  18. ncbi request reprint Evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type viruses and C-strain vaccine of Classical swine fever virus
    Jian Jun Zhao
    Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Vet Microbiol 126:1-10. 2008
    ..The novel assay described here is rapid and sensitive, and is useful for differentiating field strains and C-strain of CSFV in China...
  19. doi request reprint The role of noncoding regions of classical swine fever virus C-strain in its adaptation to the rabbit
    Chao Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Virus Res 183:117-22. 2014
    ..The results demonstrated that the NCRs of the C-strain were essential for its fever induction in rabbits and the coding region but not NCRs was necessary for its replication in the spleen of rabbits. ..
  20. ncbi request reprint [Metagenomics-based detection of swine viruses]
    Wen Han
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Wei Sheng Wu Xue Bao 53:197-203. 2013
    ..Extreme varieties of viruses exist in the environment and animals, some of which are unknown. However, many unknown viruses are barely detected by means of conventional virus isolation and PCR assay...
  21. ncbi request reprint [Expression of porcine interferon-gamma and its safe antiviral assay]
    Fan He
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 26:439-47. 2010
    ....
  22. doi request reprint Identification of a linear epitope on the capsid protein of classical swine fever virus
    Xin Zhang
    Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, 150001 Harbin, Heilongjiang, China
    Virus Res 156:134-40. 2011
    ..Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication...
  23. ncbi request reprint Identification of a conserved linear B-cell epitope at the N-terminus of the E2 glycoprotein of Classical swine fever virus by phage-displayed random peptide library
    Wu Ping Peng
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Virus Res 135:267-72. 2008
    ..The identified epitope will be useful for development of diagnostic assays and epitope-based marker vaccines against CSFV...
  24. doi request reprint Efficient and stable rescue of classical swine fever virus from cloned cDNA using an RNA polymerase II system
    Chao Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, Heilongjiang, China
    Arch Virol 158:901-7. 2013
    ..The improved reverse genetics method developed in the present study is efficient, stable, convenient and cost-effective and will be valuable for the rapid recovery of CSFV mutants...
  25. pmc Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth
    Dan Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
    J Virol 87:2072-80. 2013
    ..Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N(pro). Our results suggest that cellular PCBP1 positively modulates CSFV growth...
  26. doi request reprint Development of a triplex TaqMan real-time RT-PCR assay for differential detection of wild-type and HCLV vaccine strains of classical swine fever virus and bovine viral diarrhea virus 1
    Xing Juan Zhang
    Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Res Vet Sci 92:512-8. 2012
    ..The triplex real-time RT-PCR had at least 98% (248 samples) agreement with other RT-PCR methods. The assay provides a sensitive tool for simultaneous detection and differentiation of wild-type CSFV and HCLV from BVDV-1...
  27. ncbi request reprint [Differences in glycosylation of the E2 protein between virulent Shimen strain and a virulent C-strain of classical swine fever virus]
    Wu Ping Peng
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Bing Du Xue Bao 23:389-93. 2007
    ..We suggest that the different MW between Shimen-E2 and HCLV-E2 is resulted from the different glycosylation on 986 N glycosylation site...
  28. ncbi request reprint A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China
    Chun Hua Wang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Vaccine 32:3379-85. 2014
    ..These indicate that rPRVTJ-delgE is a promising candidate vaccine for updating Bartha-K61 for the control of the currently epidemic PR in China. ..
  29. ncbi request reprint [Progress in DNA vaccines against classical swine fever: a review]
    Na Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 26:281-9. 2010
    ..This review covered the following aspects in the development and application of CSF DNA vaccines: construction and evaluation, application of adjuvants, combination with other vaccines and the existing problems and solutions...
  30. ncbi request reprint Validation of a loop-mediated isothermal amplification assay for visualised detection of wild-type classical swine fever virus
    Xing Juan Zhang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, China
    J Virol Methods 167:74-8. 2010
    ..In summary, the LAMP assay provides a simple, rapid, and sensitive tool for the detection of wild-type CSFV under field conditions...
  31. doi request reprint In vitro inhibition of the replication of classical swine fever virus by capsid-targeted virus inactivation
    Yu Fei Wang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China
    Antiviral Res 85:422-4. 2010
    ..Thus, the CTVI approach might be applicable to CSFV inhibition as a novel antiviral strategy...
  32. ncbi request reprint [Promoter activity of different promoters in recombinant baculovirus-infected Sf9 cells]
    Yu Wang
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 24:598-603. 2008
    ..The ie1 promoter suggested the strongest promoter activity. The homologous region 1 (hr1) was also found to enhance the ETL promoter activity...
  33. doi request reprint Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus
    Fan He
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Virus Res 179:161-8. 2014
    ..Collectively, the results indicated that β-actin is involved in the early replication of CSFV. ..
  34. ncbi request reprint [Applications of reverse genetics in studying classical swine fever virus]
    Dafei Liu
    Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 25:1441-8. 2009
    ..This review focuses on the progress of applications of reverse genetics in the functional analysis and marker vaccine development of CSFV, and also discusses the problems confronted now and prospective aspects in the study of CSFV...
  35. ncbi request reprint Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells
    Hui Gao
    Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China
    J Biotechnol 131:138-43. 2007
    ..This novel shuttle promoter will facilitate the application of baculovirus-based vectors in gene expression, gene therapy, and non-replicative vector vaccines...
  36. ncbi request reprint Protection of pigs from lethal challenge by a DNA vaccine based on an alphavirus replicon expressing the E2 glycoprotein of classical swine fever virus
    Na Li
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    J Virol Methods 144:73-8. 2007
    ..Severe pathologic lesions typical of CSFV infection were observed at necropsy. It is concluded that the alphavirus replicon-vectored DNA-based vaccine can be a potential marker vaccine against CSFV...
  37. ncbi request reprint [Progress in new-type vaccines against classical swine fever]
    Chunhua Wang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, China
    Sheng Wu Gong Cheng Xue Bao 29:880-90. 2013
    ....