Linfang Wang

Summary

Affiliation: State Key Laboratory of Medical Molecular Biology
Country: China

Publications

  1. pmc A novel testis-specific GTPase serves as a link to proteasome biogenesis: functional characterization of RhoS/RSA-14-44 in spermatogenesis
    Ning Zhang
    Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    Mol Biol Cell 21:4312-24. 2010
  2. pmc GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules
    Yong Wang
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China
    BMC Cell Biol 10:25. 2009
  3. ncbi request reprint A sperm component, HSD-3.8 (SPAG1), interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK)
    Ning Liu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing, 100005, People Republic of China
    Front Biosci 11:1679-89. 2006
  4. ncbi request reprint YWK-II protein as a novel G(o)-coupled receptor for Müllerian inhibiting substance in cell survival
    Xueqian Yin
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China
    J Cell Sci 120:1521-8. 2007
  5. ncbi request reprint Crystallization and preliminary crystallographic analysis of extracellular fragment X3 of YWK-II/APPH: a human sperm membrane protein related to the Alzheimer betaA4-amyloid precursor protein
    Maojun Yang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 59:506-8. 2003
  6. ncbi request reprint Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis
    Hongyu Wu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    FEBS Lett 584:2807-15. 2010
  7. doi request reprint Regulation of apoptosis by Bat3-enhanced YWK-II/APLP2 protein stability
    Wei Wu
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, China
    J Cell Sci 125:4219-29. 2012
  8. ncbi request reprint Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-organizing centre
    Xiaoling Tang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, 100005 Beijing, China
    J Mol Med (Berl) 82:383-8. 2004
  9. doi request reprint Anti-ACTL7a antibodies: a cause of infertility
    Jun Fu
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, People s Republic of China
    Fertil Steril 97:1226-33.e1-8. 2012
  10. ncbi request reprint Delineation of the functional domains of the extracellular region of YWK-II Protein/APLP2 of sperm membrane
    Dazhong Zhuang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San, Tiao, Beijing, 100005, China
    Front Biosci 11:2371-80. 2006

Collaborators

Detail Information

Publications32

  1. pmc A novel testis-specific GTPase serves as a link to proteasome biogenesis: functional characterization of RhoS/RSA-14-44 in spermatogenesis
    Ning Zhang
    Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    Mol Biol Cell 21:4312-24. 2010
    ..Therefore, our finding uncovers a direct and functional connection between the Rho GTPase family and the pathway of proteasome biogenesis and provide new clues for deciphering the secrets of spermatogenesis...
  2. pmc GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules
    Yong Wang
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China
    BMC Cell Biol 10:25. 2009
    ..We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules...
  3. ncbi request reprint A sperm component, HSD-3.8 (SPAG1), interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK)
    Ning Liu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing, 100005, People Republic of China
    Front Biosci 11:1679-89. 2006
    ..These results provide evidence that HSD-3.8 present in spermatocytes and sperm may participate in spermatogenesis and fertilization process by activating the PKC-dependent ERK1/2 signal transduction pathway...
  4. ncbi request reprint YWK-II protein as a novel G(o)-coupled receptor for Müllerian inhibiting substance in cell survival
    Xueqian Yin
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China
    J Cell Sci 120:1521-8. 2007
    ..Taken together, the present study has demonstrated a new G(o)-coupled receptor for MIS in mediating ERK1/2 activation leading to anti-apoptotic activity or cell survival...
  5. ncbi request reprint Crystallization and preliminary crystallographic analysis of extracellular fragment X3 of YWK-II/APPH: a human sperm membrane protein related to the Alzheimer betaA4-amyloid precursor protein
    Maojun Yang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 59:506-8. 2003
    ..2, b = 44.0, c = 88.3 A, alpha = gamma = 90.0, beta = 103.6 degrees. The presence of one molecule per asymmetric unit gives a crystal Volume per protein mass (V(M)) of 2.8 A(3) Da(-1) and a solvent content of 56.4% by Volume...
  6. ncbi request reprint Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis
    Hongyu Wu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    FEBS Lett 584:2807-15. 2010
    ..These results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis...
  7. doi request reprint Regulation of apoptosis by Bat3-enhanced YWK-II/APLP2 protein stability
    Wei Wu
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, China
    J Cell Sci 125:4219-29. 2012
    ....
  8. ncbi request reprint Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-organizing centre
    Xiaoling Tang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, 100005 Beijing, China
    J Mol Med (Berl) 82:383-8. 2004
    ..These findings suggest that hSMP-1 interacts with tubulins and thereby may modulate microtubule assembly and/or activity...
  9. doi request reprint Anti-ACTL7a antibodies: a cause of infertility
    Jun Fu
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, People s Republic of China
    Fertil Steril 97:1226-33.e1-8. 2012
    ..To show that antibodies against ACTL7a, a spermatozoon-specific protein, may be a cause of immunologic infertility...
  10. ncbi request reprint Delineation of the functional domains of the extracellular region of YWK-II Protein/APLP2 of sperm membrane
    Dazhong Zhuang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San, Tiao, Beijing, 100005, China
    Front Biosci 11:2371-80. 2006
    ..The present results suggest that segment 22-308 of YWK-II-763 participates in the binding and fusion of sperm and egg plasma membranes thereby promoting fertilization...
  11. doi request reprint A novel testis protein, RSB-66, interacting with INCA1 (inhibitor of Cdk interacting with cyclin A1)
    Xu Chen
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    Biochem Cell Biol 86:345-51. 2008
    ..The alpha helix in the RSB-66 C terminal and two amino acid residues (tyr117 and his119) appear to be crucial for its function...
  12. ncbi request reprint YWK-II protein/APLP2 in mouse gametes: potential role in fertilization
    Dazhong Zhuang
    National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
    Mol Reprod Dev 73:61-7. 2006
    ..The occurrence of the YWK-II protein in the plasma membranes of mouse gametes suggests its involvement in sperm-egg interaction...
  13. doi request reprint Experimental immunological infertility effect of anti-GAPDH-2 antibodies on the fertility of female mice
    Yong Wang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, People s Republic of China
    Fertil Steril 92:2020-7. 2009
    ..To examine the relationship between an antibody against GAPDH-2, a sperm-specific protein, and infertility of female mice...
  14. doi request reprint Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa
    Jun Fu
    State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Tsinghua University, Beijing, People s Republic of China
    Fertil Steril 99:882-8. 2013
    ..To demonstrate that capacitation in mouse spermatozoa involves alterations in the expression and localization of ACTL7a...
  15. ncbi request reprint A novel gene, RSD-3/HSD-3.1, encodes a meiotic-related protein expressed in rat and human testis
    Xiaodong Zhang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, 100005 Beijing, China
    J Mol Med (Berl) 81:380-7. 2003
    ..1 gene were determined by aligning the cDNA sequence with the corresponding genome sequence. The cDNA consisted of 12 exons that span approximately 52.8 kb of the genome sequence and was mapped to chromosome 14q31.3...
  16. pmc hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37
    Xiao Bo Qiu
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, 5 Dongdan Santiao, Beijing, China
    EMBO J 25:5742-53. 2006
    ..Thus in human 26S proteasomes, hRpn13 appears to be important for the binding of UCH37 to the 19S complex and for efficient proteolysis...
  17. doi request reprint Rhomboid domain containing 1 inhibits cell apoptosis by upregulating AP-1 activity and its downstream target Bcl-3
    Xiaoxia Ren
    State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China
    FEBS Lett 587:1793-8. 2013
    ..Furthermore, knockdown of RHBDD1 led to increased cell apoptosis and reduced expression of Bcl-3. Our findings indicate that RHBDD1 could inhibit cell apoptosis by activating and upregulating c-Jun and its downstream target, Bcl-3...
  18. doi request reprint Structure of the nucleotide-binding domain of a dipeptide ABC transporter reveals a novel iron-sulfur cluster-binding domain
    Xiaolu Li
    State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing 100005, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 69:256-65. 2013
    ..The crystal structure provides a basis for understanding the properties of ABC transporters and will be helpful in investigating the functions of NBDs in the regulation of ABC transporter activity...
  19. pmc Exosome-related multi-pass transmembrane protein TSAP6 is a target of rhomboid protease RHBDD1-induced proteolysis
    Chunhua Wan
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, China
    PLoS ONE 7:e37452. 2012
    ..Taken together, our findings suggest that RHBDD1 is involved in the regulation of a nonclassical exosomal secretion pathway through the restriction of TSAP6...
  20. doi request reprint Cyclin T2: a novel miR-15a target gene involved in early spermatogenesis
    Yu Teng
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    FEBS Lett 585:2493-500. 2011
    ..Subsequently, miR-15a and Ccnt2 were profiled in developing mice testes to observe their inverse correlations in the postnatal 3-week period to understand their roles in spermatogenesis...
  21. doi request reprint Fank1 interacts with Jab1 and regulates cell apoptosis via the AP-1 pathway
    Hailong Wang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, 5 Dong Dan San Tiao, Beijing, China
    Cell Mol Life Sci 68:2129-39. 2011
    ..This process was shown to be Jab1 dependent. Taken together, our results indicated that by interacting with Jab1, Fank1 could suppress cell apoptosis by activating the AP-1-induced anti-apoptotic pathway...
  22. ncbi request reprint Crystal structure of the ectodomain of human FcalphaRI
    Yi Ding
    Ministry of Education Protein Science Laboratory and Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China
    J Biol Chem 278:27966-70. 2003
    ..Our data show that FcalphaRI, KIRs, and LIRs share a common hydrophobic core in their interdomain interface, and FcalphaRI is evolutionally closer to LIR than KIR...
  23. ncbi request reprint Identification and characterization of rDJL, a novel member of the DnaJ protein family, in rat testis
    Chumbo Yang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing
    FEBS Lett 579:5734-40. 2005
    ..The present findings suggest that rDJL functions as co-chaperone to Hsc70, participates in vesicular trafficking and may play an important role in acrosomogenesis...
  24. doi request reprint Nemo-like kinase promotes etoposide-induced apoptosis of male germ cell-derived GC-1 cells in vitro
    Xiaowen Cheng
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    FEBS Lett 586:1497-503. 2012
    ..Our findings suggest that NLK plays an important role in etoposide-induced germ cell apoptosis and may be associated with spermatogenesis...
  25. pmc Lysine-specific demethylase 1 (LSD1/KDM1A) contributes to colorectal tumorigenesis via activation of the Wnt/β-catenin pathway by down-regulating Dickkopf-1 (DKK1) [corrected]
    Zebin Huang
    National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China
    PLoS ONE 8:e70077. 2013
    ..Our studies show that LSD1 activates the Wnt/β-catenin signaling pathway by down-regulating the pathway antagonist DKK1, which may be one of the mechanisms leading to colorectal tumorigenesis. ..
  26. doi request reprint Characterisation of human RING finger protein TRIM69, a novel testis E3 ubiquitin ligase and its subcellular localisation
    Yongqing Han
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing 100005, China
    Biochem Biophys Res Commun 429:6-11. 2012
    ..Collectively, hTRIM69 is a novel E3 ubiquitin ligase identified from human testis and may function to ubiquitinate its particular substrates during spermatogenesis...
  27. pmc Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19)
    Shan Lu
    State Key Laboratory of Medical Molecular Biology, Dept of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
    PLoS ONE 9:e92712. 2014
    ..Taken together, these findings suggest that SKAP promotes UV-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19. ..
  28. doi request reprint Purification and crystallization of the ABC-type transport substrate-binding protein OppA from Thermoanaerobacter tengcongensis
    Jinlan Gao
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing 100005, People s Republic of China
    Biochem Biophys Res Commun 423:45-9. 2012
    ..Crystal diffraction extended to 2.25 Å. The crystal belonged to space group C222(1), with unit-cell parameters of a=69.395, b=199.572, c=131.673 Å, and α=β=γ=90°...
  29. ncbi request reprint Characterization and potential function of a novel testis-specific nucleoporin BS-63
    Ying Cai
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Dong Dan San Tiao, Beijing, China
    Mol Reprod Dev 61:126-34. 2002
    ..The complex performs the task of a carrier system in transporting aF10 into the nucleus of germ cells during spermiogenesis...
  30. pmc The protective effect of Bcl-xl overexpression against oxidative stress-induced vascular endothelial cell injury and the role of the Akt/eNOS pathway
    Leng Ni
    Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, 1 Shuaifuyuan, Dongcheng District, Beijing 100730, China
    Int J Mol Sci 14:22149-62. 2013
    ..These findings suggest that Bcl-xl overexpression exerts an anti-apoptotic and protective effect on EC function. The Akt/eNOS signaling pathway is probably involved in these processes. ..
  31. ncbi request reprint Crystallization and preliminary crystallographic analysis of a partial extracellular fragment of a sperm membrane protein YWK-II/APPH related to the Alzheimer betaA4-amyloid precursor protein
    Maojun Yang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 59:146-9. 2003
    ..51 A(3) Da(-1) and a solvent content of 64.6% by Volume. A full set of X-ray diffraction data were collected to 2.8 A resolution from the native crystal...
  32. pmc Frequent epigenetic silencing of the folate-metabolising gene cystathionine-beta-synthase in gastrointestinal cancer
    Hong Zhao
    Department of Abdominal Surgical Oncology, Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
    PLoS ONE 7:e49683. 2012
    ..Cystathionine-beta-synthase (CBS) functions in the folate metabolism pathway, which is intricately linked to methylation of genomic DNA. Dysregulation of DNA methylation contributes substantially to cancer development...