Jin K Wang

Summary

Affiliation: Southeast University
Country: China

Publications

  1. pmc Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins
    Jinke Wang
    Chien Shiung WU Laboratory, Southeast University Nanjing 210096, China
    Nucleic Acids Res 33:e23. 2005
  2. pmc An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-κB as an example
    Guangming Gu
    The State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China
    PLoS ONE 8:e76109. 2013
  3. doi request reprint Near-infrared fluorescence labeling of iron nanoparticles and applications for cell labeling and in vivo imaging
    Jinke Wang
    Experimental Center of Biotechnology and Biomaterials, BME, Southeast University, Nanjing, China
    Methods Mol Biol 906:221-37. 2012
  4. ncbi request reprint Assay of DNA-binding proteins with a dsDNA-coupled plate
    Jin K Wang
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    Clin Biochem 39:167-75. 2006
  5. pmc Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging
    Yong Hou
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    J Nanobiotechnology 8:25. 2010
  6. ncbi request reprint A method for fabricating uni-dsDNA microarray chip for analyzing DNA-binding proteins
    Jin K Wang
    Chien Shiung WU Laboratory, Southeast University, Nanjing 210096, PR China
    J Biochem Biophys Methods 63:100-10. 2005
  7. doi request reprint In vitro DNA-binding profile of transcription factors: methods and new insights
    Jinke Wang
    The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People s Republic of China
    J Endocrinol 210:15-27. 2011
  8. ncbi request reprint Exonuclease-mediated ELISA-like assay for detecting DNA-binding activity of transcription factors: measurement of activated NF-kappaB
    Jinke Wang
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China
    Biotechniques 41:79-88, 90. 2006
  9. ncbi request reprint Evaluating the binding affinities of NF-kappaB p50 homodimer to the wild-type and single-nucleotide mutant Ig-kappaB sites by the unimolecular dsDNA microarray
    Jin K Wang
    Department of Biomedical Engineering, Chien Shiung WU Laboratory, Southeast University, Nanjing 210096, China
    Anal Biochem 316:192-201. 2003
  10. ncbi request reprint In vitro cellular uptake and effects of Fe3O4 magnetic nanoparticles on HeLa cells
    Shanshan Shen
    Department of Clinical Medical College, Southeast University, Nanjing 210096, China
    J Nanosci Nanotechnol 9:2866-71. 2009

Collaborators

Detail Information

Publications16

  1. pmc Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins
    Jinke Wang
    Chien Shiung WU Laboratory, Southeast University Nanjing 210096, China
    Nucleic Acids Res 33:e23. 2005
    ..This assay can be extensively used in biomedical research targeted at DNA-binding proteins...
  2. pmc An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-κB as an example
    Guangming Gu
    The State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China
    PLoS ONE 8:e76109. 2013
    ..Therefore, this study provided a useful SELEX-Seq strategy for characterizing DNA-binding specificities of transcription factors. ..
  3. doi request reprint Near-infrared fluorescence labeling of iron nanoparticles and applications for cell labeling and in vivo imaging
    Jinke Wang
    Experimental Center of Biotechnology and Biomaterials, BME, Southeast University, Nanjing, China
    Methods Mol Biol 906:221-37. 2012
    ....
  4. ncbi request reprint Assay of DNA-binding proteins with a dsDNA-coupled plate
    Jin K Wang
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    Clin Biochem 39:167-75. 2006
    ..This paper fabricated a cost-effective dsDNA-coupled plate (dcPlate) and applied it to measure the abundance and DNA-binding activity of a DNA-binding protein (DBP)...
  5. pmc Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging
    Yong Hou
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    J Nanobiotechnology 8:25. 2010
    ..abstract:..
  6. ncbi request reprint A method for fabricating uni-dsDNA microarray chip for analyzing DNA-binding proteins
    Jin K Wang
    Chien Shiung WU Laboratory, Southeast University, Nanjing 210096, PR China
    J Biochem Biophys Methods 63:100-10. 2005
    ..The uni-dsDNA microarray was verified applicable for detecting the presence and monitoring the DNA-binding activity of the sequence-specific DNA-binding proteins...
  7. doi request reprint In vitro DNA-binding profile of transcription factors: methods and new insights
    Jinke Wang
    The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People s Republic of China
    J Endocrinol 210:15-27. 2011
    ..This study reviewed these techniques, especially two master techniques: double-stranded DNA microarray and systematic evolution of ligands by exponential enrichment in combination with parallel DNA sequencing techniques (SELEX-seq)...
  8. ncbi request reprint Exonuclease-mediated ELISA-like assay for detecting DNA-binding activity of transcription factors: measurement of activated NF-kappaB
    Jinke Wang
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China
    Biotechniques 41:79-88, 90. 2006
    ..EMEA may provide a general approach for assays of DNA sequence-specific transcription factors for which specific antibodies are unavailable, expensive, or of insufficient quality...
  9. ncbi request reprint Evaluating the binding affinities of NF-kappaB p50 homodimer to the wild-type and single-nucleotide mutant Ig-kappaB sites by the unimolecular dsDNA microarray
    Jin K Wang
    Department of Biomedical Engineering, Chien Shiung WU Laboratory, Southeast University, Nanjing 210096, China
    Anal Biochem 316:192-201. 2003
    ..The unimolecular dsDNA microarray provides a reliable method for exploring the binding affinities of DNA-binding proteins with a larger number of DNA targets...
  10. ncbi request reprint In vitro cellular uptake and effects of Fe3O4 magnetic nanoparticles on HeLa cells
    Shanshan Shen
    Department of Clinical Medical College, Southeast University, Nanjing 210096, China
    J Nanosci Nanotechnol 9:2866-71. 2009
    ..Therefore, it is concluded that the Fe3O4 MNPs have a good biocompatibility to HeLa cells cultured in vitro and do not affect NF-kappaB signaling pathway under the dose and acting time used in this study...
  11. ncbi request reprint Evaluating the binding affinities of NF-kappaB protein to the single-nucleotide mismatch DNA binding sites by using double-stranded DNA microarray
    Yunfei Bai
    State Key Laboratory for Bioelectronics, Southeast University, Nanjing, China
    J Nanosci Nanotechnol 6:1014-8. 2006
    ....
  12. ncbi request reprint Optimization of on-chip elongation for fabricating double-stranded DNA microarrays
    Yunfei Bai
    Chien Shiung WU Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
    Colloids Surf B Biointerfaces 40:153-8. 2005
    ..5 mM are the optimized conditions in elongation with Taq DNA polymerase. A dsDNA microarray has been successfully constructed with the above method to detect NF-kB protein...
  13. ncbi request reprint Investigation of DNA-protein sequence-specific interactions with a ds-DNA array
    Yunfei Bai
    Chien Shiung WU Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China
    Molecules 10:417-26. 2005
    ..Our results indicated that the ds-DNA arrays can be further biochemically modified and made accessible for interactions between DNAs and proteins in complex multi-step gene-regulation processes...
  14. ncbi request reprint A free-labeled method for DNA-binding protein detection using a double-stranded DNA microarray
    Yunfei Bai
    Department of Biological Science and Medical Engineering, Key Lab of Molecular and Biomolecular Electronics, Ministry of Education, Southeast University, Nanjing 210096, P R China
    J Nanosci Nanotechnol 5:1216-9. 2005
    ..The detection results suggested that this free-labeled detection system could have the ability to be used in research and medical diagnosis and for high-throughput screening of drugs targeted to DNA-binding proteins...
  15. ncbi request reprint Accurate identification of closely related Dendrobium species with multiple species-specific gDNA probes
    Tongxiang Li
    Chien Shiung WU Laboratory, Southeast University, Nanjing 210096, P R China
    J Biochem Biophys Methods 62:111-23. 2005
    ....
  16. ncbi request reprint DNA microarrays with unimolecular hairpin double-stranded DNA probes: fabrication and exploration of sequence-specific DNA/protein interactions
    Jinke Wang
    National Laboratory for Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096 Jiangsu Province, China
    J Biochem Biophys Methods 55:215-32. 2003
    ..The dsDNA microarrays have great potential to provide a high-throughput platform for investigation of sequence-specific DNA/protein interactions involved in gene expression regulation, restriction and so on...