Youhua Huang

Summary

Affiliation: South China Sea Institute of Oceanology
Country: China

Publications

  1. pmc Transcriptome analysis of orange-spotted grouper (Epinephelus coioides) spleen in response to Singapore grouper iridovirus
    Youhua Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, PR China
    BMC Genomics 12:556. 2011
  2. doi request reprint Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides
    Zhengliang Ou-Yang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Dev Comp Immunol 38:254-61. 2012
  3. doi request reprint Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Fish Shellfish Immunol 34:1094-102. 2013
  4. doi request reprint Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides
    Shina Wei
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    Fish Shellfish Immunol 33:186-96. 2012
  5. ncbi request reprint Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination
    Zhengliang Ou-Yang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Vet Immunol Immunopathol 149:38-45. 2012
  6. doi request reprint Singapore grouper iridovirus, a large DNA virus, induces nonapoptotic cell death by a cell type dependent fashion and evokes ERK signaling
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    Apoptosis 16:831-45. 2011
  7. ncbi request reprint Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses
    Yang Yan
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    J Gen Virol 95:1144-55. 2014
  8. doi request reprint Characterization of p38 MAPKs from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
    Jia Cai
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Fish Shellfish Immunol 31:1129-36. 2011
  9. doi request reprint Roles of stress-activated protein kinases in the replication of Singapore grouper iridovirus and regulation of the inflammatory responses in grouper cells
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    J Gen Virol 92:1292-301. 2011
  10. doi request reprint Antiviral effects of β-defensin derived from orange-spotted grouper (Epinephelus coioides)
    Minglan Guo
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    Fish Shellfish Immunol 32:828-38. 2012

Collaborators

  • Sheng Zhou
  • Hong Liu
  • Shaowen Wang
  • Qiwei Qin
  • Xiaohong Huang
  • Shina Wei
  • Jia Cai
  • Yang Yan
  • Zhengliang Ouyang
  • Jingguang Wei
  • Ren Gao
  • Jing Fu
  • Zhengliang Ou-Yang
  • Yongcan Zhou
  • Pengfei Li
  • Chuanyu Guo
  • Fuzhou Ye
  • Huachun Cui
  • Minglan Guo
  • Peiran Wang
  • Lili Li
  • Guohua Jiang
  • Liya Guan
  • Xiuli Chen
  • Jie Gong
  • Qingjiao Wan
  • Huasong Ji
  • Lixiao Xu
  • Xin Han

Detail Information

Publications20

  1. pmc Transcriptome analysis of orange-spotted grouper (Epinephelus coioides) spleen in response to Singapore grouper iridovirus
    Youhua Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, PR China
    BMC Genomics 12:556. 2011
    ..In this study, we used a 454 pyrosequencing method to investigate differentially-expressed genes in the spleen of the E. coioides infected with Singapore grouper iridovirus (SGIV)...
  2. doi request reprint Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides
    Zhengliang Ou-Yang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Dev Comp Immunol 38:254-61. 2012
    ..Taken together, our results clearly demonstrated that effective vaccination in grouper induced an early, nonspecific antiviral immunity, and later, a specific immune response involving both humoral and cell-mediated immunity...
  3. doi request reprint Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Fish Shellfish Immunol 34:1094-102. 2013
    ..Furthermore, overexpression of EcISG15 in vitro inhibited the transcription of GNNV genes significantly. Taken together, the results indicated that fish ISG15 might exert important roles against RNA virus infection...
  4. doi request reprint Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides
    Shina Wei
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    Fish Shellfish Immunol 33:186-96. 2012
    ..Taken together, Ec-lysC might play an important role in grouper innate immune responses to invasion of bacterial and viral pathogens. C-type lysozyme gene from E. coioides (Ec-lysC) was identified and characterized...
  5. ncbi request reprint Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination
    Zhengliang Ou-Yang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Vet Immunol Immunopathol 149:38-45. 2012
    ..The promising results obtained in the present study have prompted further testing to improve the immunogenicity of these potential DNA vaccines...
  6. doi request reprint Singapore grouper iridovirus, a large DNA virus, induces nonapoptotic cell death by a cell type dependent fashion and evokes ERK signaling
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    Apoptosis 16:831-45. 2011
    ..This is a first demonstration of ERK-mediated nonapoptotic cell death induced by a DNA virus. These findings contribute to understanding the mechanisms of iridovirus pathogenesis...
  7. ncbi request reprint Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses
    Yang Yan
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    J Gen Virol 95:1144-55. 2014
    ..Ultimately, our study identified a novel, functional SGIV gene that could regulate cytoskeletal structure, immune responses and facilitate viral replication. ..
  8. doi request reprint Characterization of p38 MAPKs from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
    Jia Cai
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Fish Shellfish Immunol 31:1129-36. 2011
    ..Taken together, our data indicated that Ec-p38β played a crucial role in regulating apoptosis and virus replication during iridovirus infection...
  9. doi request reprint Roles of stress-activated protein kinases in the replication of Singapore grouper iridovirus and regulation of the inflammatory responses in grouper cells
    Xiaohong Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    J Gen Virol 92:1292-301. 2011
    ..It is proposed that the JNK pathway is important for SGIV replication and modulates the inflammatory responses during virus infection...
  10. doi request reprint Antiviral effects of β-defensin derived from orange-spotted grouper (Epinephelus coioides)
    Minglan Guo
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China
    Fish Shellfish Immunol 32:828-38. 2012
    ..These results suggest that EcDefensin is importantly involved in host immune responses to invasion of viral pathogens, and open the new avenues for design of antiviral agents in fisheries industry...
  11. ncbi request reprint Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides
    Jing Fu
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China University of Chinese Academy of Sciences, Beijing, PR China
    Fish Shellfish Immunol 36:19-26. 2014
    ..In addition, far western blotting assay revealed that Ec-Rab7 might interact with viral proteins, including SGIV VP69 and VP101. Taken together, our data suggested that Ec-Rab7 might be potentially involved in SGIV replication. ..
  12. doi request reprint Construction of green fluorescent protein-tagged recombinant iridovirus to assess viral replication
    Youhua Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China
    Virus Res 160:221-9. 2011
    ..Thus, the EGFP-tagged recombinant iridovirus will not only be useful for further investigations on the viral replicative dynamics, but also provide an alternative simple strategy to screen for antiviral substances...
  13. ncbi request reprint Identification and characterization of a tumor necrosis factor receptor like protein encoded by Singapore grouper iridovirus
    Xiaohong Huang
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Virus Res 178:340-8. 2013
    ..Taken together, our results suggested that SGIV might utilize virus encoded TNFR like genes to modulate the host apoptotic response for effective virus replication. ..
  14. ncbi request reprint Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities
    Pengfei Li
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China
    Virus Res 188:146-54. 2014
    ....
  15. ncbi request reprint Molecular cloning and characterization of two types of IκBα orthologues in orange-spotted grouper, Epinephelus coioides
    Ren Gao
    State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, College of Marine Science, Hainan University, Haikou 570228, China Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Fish Shellfish Immunol 38:101-10. 2014
    ..Taken together, our findings suggested that both EcIκBαA and EcIκBαB were involved in grouper innate immunity against virus. ..
  16. doi request reprint Characterization of LPS-induced TNFα factor (LITAF) from orange-spotted grouper, Epinephelus coioides
    Jia Cai
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China
    Fish Shellfish Immunol 35:1858-66. 2013
    ..Taken together, our data suggested that Ec-LITAF might play crucial roles during SGIV replication. ..
  17. ncbi request reprint Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
    Shina Wei
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Fish Shellfish Immunol 36:194-205. 2014
    ..Additionally, SGIV-induced typical apoptosis was also inhibited by CA-074Me in FHM cells. Taken together, our results demonstrated that the Ec-CB might play a functional role in SGIV infection. ..
  18. pmc Characterization of an envelope gene VP19 from Singapore grouper iridovirus
    Xiaohong Huang
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
    Virol J 10:354. 2013
    ..Although numerous iridovirus envelope proteins have been identified using bioinformatics and proteomic methods, their roles in virus infection remained largely unknown...
  19. doi request reprint Proteomic analysis of Singapore grouper iridovirus envelope proteins and characterization of a novel envelope protein VP088
    Sheng Zhou
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Guangzhou, P R China
    Proteomics 11:2236-48. 2011
    ..Neutralization assay also suggested that VP088 is involved in SGIV infection. This study will lead to a better understanding of molecular mechanisms of the iridoviral pathogenesis and virus-host interactions...
  20. doi request reprint Involvement of the mitogen-activated protein kinase pathway in soft-shelled turtle iridovirus-induced apoptosis
    Youhua Huang
    Key Laboratory of Marine Bio resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China
    Apoptosis 16:581-93. 2011
    ..Taken together, our findings shed light on the roles of the MAPK signaling pathway in iridovirus-induced apoptosis and virus replication, which provides new insights into understanding iridovirus-host interaction...