Chun Yang Zhang

Summary

Affiliation: Shandong Normal University
Country: China

Publications

  1. Cui L, Wang M, Sun B, Ai S, Wang S, Zhang C. Substrate-free and label-free electrocatalysis-assisted biosensor for sensitive detection of microRNA in lung cancer cells. Chem Commun (Camb). 2019;55:1172-1175 pubmed publisher
    ..53 × 10-16 M and a large dynamic range of 6 orders of magnitude. It can be further applied for accurate quantification of microRNA in lung cancer cells. ..
  2. Ma F, Liu H, Li C, Zhang C. A simple and isothermal ligase-based amplification approach based on a ligation-activated cleavage reaction. Chem Commun (Camb). 2018;54:12638-12641 pubmed publisher
    ..As a proof of concept, we demonstrate the application of this method for the sensitive detection of polynucleotide kinase. Moreover, this method can be extended to the accurate quantification of DNA, proteins and ATP. ..
  3. Wang L, Han X, Li C, Zhang C. Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase. Chem Sci. 2018;9:6053-6061 pubmed publisher
    ..coli cells, and the screening of Dam MTase inhibitors, providing a new paradigm for biomedical research and clinical diagnosis. ..
  4. Wang Z, Wang M, Zhang Y, Zhang C. Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification. Chem Commun (Camb). 2018;54:8602-8605 pubmed publisher
    ..This method exhibits high sensitivity with a detection limit of as low as 34.8 fM. It can discriminate as low as the 0.01% 5hmC level from the mixture, and exhibits good performance in serum sample analysis as well. ..
  5. Wang Z, Wang L, Zhang Q, Tang B, Zhang C. Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites. Chem Sci. 2018;9:1330-1338 pubmed publisher
    ..Moreover, this nanosensor can distinguish as low as a 0.01% methylation level, and it can detect DNA methylation in human lung cancer cells as well, holding great potential for accurate epigenetic evaluation and early cancer diagnosis. ..
  6. Hu J, Liu M, Li Y, Tang B, Zhang C. Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level. Chem Sci. 2018;9:712-720 pubmed publisher
    ..Moreover, this method can be used for the screening of enzyme inhibitors and the simultaneous detection of hOGG1 and hAAG from lung cancer cells, having great potential for further application in early clinical diagnosis. ..
  7. Ma F, Liu M, Wang Z, Zhang C. Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection. Chem Commun (Camb). 2016;52:1218-21 pubmed publisher
  8. Ma F, Liu W, Liang L, Tang B, Zhang C. Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification. Chem Commun (Camb). 2018;54:2413-2416 pubmed publisher
    ..Importantly, this method can be further applied for kinetic analysis and ALP inhibition assay. ..
  9. Wang L, Wang Z, Zhang Q, Tang B, Zhang C. Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase. Chem Commun (Camb). 2017;53:3878-3881 pubmed publisher
    ..This method exhibits excellent sensitivity with a detection limit of 5.6 × 10-7 U μL-1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells. ..

More Information

Publications37

  1. Wang L, Ma F, Tang B, Zhang C. Sensing telomerase: From in vitro detection to in vivo imaging. Chem Sci. 2017;8:2495-2502 pubmed publisher
    ..In this Minireview, we summarize the emerging strategies for telomerase assays in the last five years, including both in vitro assays and in vivo imaging methods, and discuss their future directions as well. ..
  2. Wang L, Wang Z, Zhang C. Primer dephosphorylation-initiated circular exponential amplification for ultrasensitive detection of alkaline phosphatase. Analyst. 2018;143:4606-4613 pubmed publisher
    ..Importantly, this method can be applied for the measurement of kinetic parameters and the screening of potential inhibitors, providing a powerful tool for ALP-related biomedical research and clinical diagnosis. ..
  3. Wang L, Ren M, Liang L, Zhang C. Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic viruses. Chem Sci. 2018;9:4942-4949 pubmed publisher
  4. Hu J, Liu M, Zhang C. Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs. Chem Sci. 2018;9:4258-4267 pubmed publisher
    ..2 × 10-16 M for miR-21 and 1.6 × 10-17 M for miR-221, and it can be used for simultaneous detection of multiple miRNAs in human cancer cells, holding great potential in biomedical research and clinical diagnosis. ..
  5. Dong J, Xu Q, Li C, Zhang C. Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification. Chem Commun (Camb). 2019;55:2457-2460 pubmed publisher
    ..2 °C) without the involvement of either multicolor labels or parallelized multiplexing, and it can sensitively detect LAMP amplicons with the initial DNA concentrations ranging from 10 to 108 copies. ..
  6. Ma F, Wang T, Jiang L, Zhang C. Ultrasensitive detection of telomerase activity in lung cancer cells with quencher-free molecular beacon-assisted quadratic signal amplification. Anal Chim Acta. 2019;1053:122-130 pubmed publisher
    ..Moreover, this method can be further applied for telomerase inhibition assay and the discrimination of cancer cells from normal cells, holding great potential in telomerase-related clinical diagnosis and drug discovery. ..
  7. Zhang D, Ma F, Leng J, Zhang C. A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity. Chem Commun (Camb). 2018;54:13678-13681 pubmed publisher
    ..This biosensor can achieve ultrahigh sensitivity with a detection limit of 0.35 pM, and it can be further used for the screening of Ago2 inhibitors and the accurate measurement of endogenous Ago2 from HeLa cell extracts. ..
  8. Liu W, Xu Q, Ma F, Li C, Zhang C. Exonuclease III-assisted multiple cycle amplification for the sensitive detection of DNA with zero background signal. Analyst. 2018;143:5461-5466 pubmed publisher
  9. Xu Q, Zhang Y, Xiang D, Li C, Zhang C. A universal DNAzyme-based bioluminescent sensor for label-free detection of biomolecules. Anal Chim Acta. 2018;1043:81-88 pubmed publisher
  10. Cui L, Hu J, Li C, Wang C, Zhang C. An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework. Biosens Bioelectron. 2018;122:168-174 pubmed publisher
    ..Moreover, this biosensor can be used to screen the PNK inhibitors, holding great potential in disease diagnosis and drug discovery. ..
  11. Li C, Hu J, Lu M, Zhang C. Quantum dot-based electrochemical biosensor for stripping voltammetric detection of telomerase at the single-cell level. Biosens Bioelectron. 2018;122:51-57 pubmed publisher
  12. Liu M, Ma F, Zhang Q, Zhang C. Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level. Chem Commun (Camb). 2018;54:1583-1586 pubmed publisher
    ..Importantly, this method enables sensitive detection of endogenous PNK activity at the single-cell level, holding great potential in clinical diagnosis and biomedical research. ..
  13. Li C, Zhang Y, Liu W, Zhang C. A triple-amplification strategy for sensitive detection of telomerase at the single-cell level. Chem Commun (Camb). 2018;54:9317-9320 pubmed publisher
    ..This method can detect telomerase at the single-cell level, and it can be further applied for the screening of telomerase inhibitors for anticancer drug discovery. ..
  14. Cui L, Li C, Tang B, Zhang C. Advances in the integration of quantum dots with various nanomaterials for biomedical and environmental applications. Analyst. 2018;143:2469-2478 pubmed publisher
    ..In addition, we discuss the sensing and imaging mechanisms of different strategies and give new insight into the challenges and future direction as well. ..
  15. Li C, Zhang Y, Tang B, Zhang C. Integration of single-molecule detection with magnetic separation for multiplexed detection of DNA glycosylases. Chem Commun (Camb). 2018;54:5839-5842 pubmed publisher
    ..This method can sensitively detect multiple DNA glycosylases, and it can be further applied for the simultaneous measurement of enzyme kinetic parameters and screening of both hOGG1 and UDG inhibitors. ..
  16. Xu A, Yang Y, Zhang C. Transpeptidation-directed intramolecular bipartite tetracysteine display for sortase activity assay. Chem Commun (Camb). 2018;54:8116-8119 pubmed publisher
    ..This assay is homogenous with a detection limit of 10.7 ng mL-1, and it can be applied to characterize Sortase A (SrtA)-targeted inhibitors, providing a new approach for anti-virulence drug discovery. ..
  17. Zhang D, Hu J, Yang X, Wu Y, Su W, Zhang C. Target-initiated synthesis of fluorescent copper nanoparticles for the sensitive and label-free detection of bleomycin. Nanoscale. 2018;10:11134-11142 pubmed publisher
    ..1 × 10-16 M, and it exhibits good performance in serum samples. Moreover, this method has distinct advantages of simplicity and low cost, holding great potential in clinical diagnosis and biomedical research. ..
  18. Zhang Y, Li Q, Li C, Zhang C. Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification. Chem Commun (Camb). 2018;54:6991-6994 pubmed publisher
    ..This method exhibits high sensitivity with a detection limit as low as 0.00031 U mL-1, and it can be further applied for the measurement of enzyme kinetic parameters and the screening of UDG inhibitors as well as cancer cell analysis. ..
  19. Hu J, Wang Z, Li C, Zhang C. Advances in single quantum dot-based nanosensors. Chem Commun (Camb). 2017;53:13284-13295 pubmed publisher
    ..We highlight the challenges and future direction of single QD-based nanosensors as well. ..
  20. Zhang Y, Ma F, Tang B, Zhang C. Recent advances in transcription factor assays in vitro. Chem Commun (Camb). 2016;52:4739-48 pubmed publisher
    ..In this paper, we review the recent advances in TF assays and highlight the emerging trends as well. ..
  21. Ma F, Wei S, Leng J, Tang B, Zhang C. A simple "mix-and-detection" method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions. Chem Commun (Camb). 2018;54:2483-2486 pubmed publisher
    ..This method enables one-step and one-tube detection of telomerase with a detection limit of 3 cells, and it can be further applied for the screening of telomerase inhibitors and the discrimination of cancer cells from normal cells. ..
  22. Cui L, Lu M, Li Y, Tang B, Zhang C. A reusable ratiometric electrochemical biosensor on the basis of the binding of methylene blue to DNA with alternating AT base sequence for sensitive detection of adenosine. Biosens Bioelectron. 2018;102:87-93 pubmed publisher
  23. Zhang D, Ma F, Zhang Q, Zhang C. Highly sensitive detection of epidermal growth factor receptor in lung cancer cells by aptamer-based target-/probe-mediated cyclic signal amplification. Chem Commun (Camb). 2017;53:11496-11499 pubmed publisher
    ..The method is highly sensitive with a detection limit of 0.16 fM, and it can be applied to detect EGFR in lung cancer cells, holding great potential in clinical diagnosis. ..
  24. Cui L, Li Y, Lu M, Tang B, Zhang C. An ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification. Biosens Bioelectron. 2018;99:1-7 pubmed publisher
    ..Moreover, this method can be used to screen the PNK inhibitors, and it shows excellent performance in real sample analysis, thus holding great potential for further applications in biological researches and clinic diagnosis. ..
  25. Zhang Y, Li Y, Tang B, Zhang C. The strategies for identification and quantification of SUMOylation. Chem Commun (Camb). 2017;53:6989-6998 pubmed publisher
    ..We highlight the emerging trends in this field as well. ..
  26. Wang L, Luo M, Zhang Q, Tang B, Zhang C. Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase. Chem Commun (Camb). 2017;53:11016-11019 pubmed publisher
    ..This method is very sensitive with a detection limit as low as 1 × 10-6 U μL-1, and it can be used for the screening of TDT inhibitors and accurate quantification of TdT activity even in 5 cancer cells. ..
  27. Ma F, Liu W, Zhang Q, Zhang C. Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching. Chem Commun (Camb). 2017;53:10596-10599 pubmed publisher
    ..This method is extremely sensitive with a detection limit of 0.58 fM, and it can be applied to absolutely detect endogenous microRNAs in cancer cells, holding great potential for clinical diagnosis and biomedical research studies. ..
  28. Ma F, Liu W, Tang B, Zhang C. A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase. Chem Commun (Camb). 2017;53:6868-6871 pubmed publisher
    ..002 U mL-1, and it can be further applied for inhibitor screening and accurate detection of DNA MTase in complex biological samples. ..