Yue Zhong Li

Summary

Affiliation: Shandong University
Country: China

Publications

  1. Wang Y, Li Y. Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics. BMC Biotechnol. 2014;14:53 pubmed publisher
    ..The present study suggested a new strategy to increase the solubilization of overexpressed recombinant proteins in E. coli cells. ..
  2. Peng R, Wang Y, Feng W, Yue X, Chen J, Hu X, et al. CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus. Microb Cell Fact. 2018;17:15 pubmed publisher
    ..This is the first time to construct the CRISPR/dCas9 activation system in myxobacteria and the first time to assay the CRISPR/dCas9 activations for the biosynthesis of microbial secondary metabolites. ..
  3. Zhao J, Zhong L, Shen M, Xia Z, Cheng Q, Sun X, et al. Discovery of the autonomously replicating plasmid pMF1 from Myxococcus fulvus and development of a gene cloning system in Myxococcus xanthus. Appl Environ Microbiol. 2008;74:1980-7 pubmed publisher
    ..The transformants subsequently exhibited the ability to form pili and social motility. Autonomously replicating plasmid pMF1 provides a new tool for genetic manipulation in Myxococcus...
  4. Yue X, Cui X, Zhang Z, Peng R, Zhang P, Li Z, et al. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis. Microb Cell Fact. 2017;16:92 pubmed publisher
    ..Esi binds to the epothilone promoter and down-regulates the transcriptional level of the whole gene cluster to affect the biosynthesis of epothilone. This is the first transcription regulator identified for epothilone biosynthesis. ..
  5. Yang Y, Wang Y, Li Z, Gong Y, Zhang P, Hu W, et al. Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus. Microb Cell Fact. 2017;16:142 pubmed publisher
    ..xanthus DK1622 and its epothilone-producing mutant. The findings and the proposals described in this paper were suggested to be workable in other organisms, for example, other Gram negative bacteria with high GC content. ..

Detail Information

Publications5

  1. Wang Y, Li Y. Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics. BMC Biotechnol. 2014;14:53 pubmed publisher
    ..The present study suggested a new strategy to increase the solubilization of overexpressed recombinant proteins in E. coli cells. ..
  2. Peng R, Wang Y, Feng W, Yue X, Chen J, Hu X, et al. CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus. Microb Cell Fact. 2018;17:15 pubmed publisher
    ..This is the first time to construct the CRISPR/dCas9 activation system in myxobacteria and the first time to assay the CRISPR/dCas9 activations for the biosynthesis of microbial secondary metabolites. ..
  3. Zhao J, Zhong L, Shen M, Xia Z, Cheng Q, Sun X, et al. Discovery of the autonomously replicating plasmid pMF1 from Myxococcus fulvus and development of a gene cloning system in Myxococcus xanthus. Appl Environ Microbiol. 2008;74:1980-7 pubmed publisher
    ..The transformants subsequently exhibited the ability to form pili and social motility. Autonomously replicating plasmid pMF1 provides a new tool for genetic manipulation in Myxococcus...
  4. Yue X, Cui X, Zhang Z, Peng R, Zhang P, Li Z, et al. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis. Microb Cell Fact. 2017;16:92 pubmed publisher
    ..Esi binds to the epothilone promoter and down-regulates the transcriptional level of the whole gene cluster to affect the biosynthesis of epothilone. This is the first transcription regulator identified for epothilone biosynthesis. ..
  5. Yang Y, Wang Y, Li Z, Gong Y, Zhang P, Hu W, et al. Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus. Microb Cell Fact. 2017;16:142 pubmed publisher
    ..xanthus DK1622 and its epothilone-producing mutant. The findings and the proposals described in this paper were suggested to be workable in other organisms, for example, other Gram negative bacteria with high GC content. ..