Xiaofeng Ren

Summary

Country: China

Publications

  1. ncbi Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium
    Bin Zeng
    Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
    J Biomed Sci 17:80. 2010
  2. ncbi Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: a way for producing functional protein in prokaryotic microorganism
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China
    J Biotechnol 147:130-5. 2010
  3. ncbi Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Biotechnol Lett 33:215-20. 2011
  4. ncbi Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Virus Genes 42:229-35. 2011
  5. ncbi Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China
    J Biotechnol 150:202-6. 2010
  6. ncbi Cholesterol dependence of pseudorabies herpesvirus entry
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China
    Curr Microbiol 62:261-6. 2011
  7. ncbi Antiviral activities of phosphonoformate sodium to pseudorabies herpesvirus infection in vitro
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China or
    Pharm Biol 49:608-13. 2011
  8. ncbi Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 6:e18669. 2011
  9. ncbi Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030, Harbin, People s Republic of China
    Virus Genes 38:39-45. 2009
  10. ncbi A descriptive analysis of medical education in China
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, PR China
    Med Teach 30:667-72. 2008

Collaborators

Detail Information

Publications44

  1. ncbi Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium
    Bin Zeng
    Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
    J Biomed Sci 17:80. 2010
    ..Our current findings support the premise that HO-1 transduced by MSCs can induce angiogenic effects and improve heart function after acute myocardial infarction...
  2. ncbi Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: a way for producing functional protein in prokaryotic microorganism
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China
    J Biotechnol 147:130-5. 2010
    ..Biologically active GP5 and anti-GP5 antibody inhibited cell infection by PRRSV. Moreover, the antibody produced in this study was capable of detecting the cell infection by PRRSV and distinguishing this virus from other viruses...
  3. ncbi Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Biotechnol Lett 33:215-20. 2011
    ..Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive...
  4. ncbi Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Virus Genes 42:229-35. 2011
    ....
  5. ncbi Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China
    J Biotechnol 150:202-6. 2010
    ..The results suggest that the major antibody-binding domains of pAPN may associate with the receptor-binding determinants. The role of APN is discussed in the context of virus receptor usage...
  6. ncbi Cholesterol dependence of pseudorabies herpesvirus entry
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China
    Curr Microbiol 62:261-6. 2011
    ..Exogenous cholesterol added to either MβCD-treated cells or virions partially restored the virus infectivity. Optimal PrV infection requires cholesterol in viral and plasma membranes...
  7. ncbi Antiviral activities of phosphonoformate sodium to pseudorabies herpesvirus infection in vitro
    Xiaofeng Ren
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China or
    Pharm Biol 49:608-13. 2011
    ..Phosphonoformate sodium (PFS) has been used as an anti-herpesvirus drug; nevertheless, studies of the use of PFS for treatment of pseudorabies herpesvirus (PrV) infection in the veterinary setting have not been widely reported...
  8. ncbi Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 6:e18669. 2011
    ..Together with other reports concerning the inhibitory effect of lithium salts on IBV in cell culture, our results indicate that LiCl may be a potent agent against porcine and avian coronaviruses...
  9. ncbi Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030, Harbin, People s Republic of China
    Virus Genes 38:39-45. 2009
    ....
  10. ncbi A descriptive analysis of medical education in China
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, PR China
    Med Teach 30:667-72. 2008
    ....
  11. ncbi Production and characterization of a monoclonal antibody against spike protein of transmissible gastroenteritis virus
    Fandan Meng
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Hybridoma (Larchmt) 29:345-50. 2010
    ..Moreover, immunofluorescence assays showed that the MAb is able to detect cell infection by TGEV. The MAb achieved in this study can be used as a specific diagnostic reagent for detecting TGEV S protein...
  12. ncbi Development and evaluation of a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of type II porcine reproductive and respiratory syndrome virus
    Ming Gao
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    J Virol Methods 185:18-23. 2012
    ..In addition, the RT-LAMP assay was capable of detecting type II PRRSV in field samples and differentiating type II PRRSV from seven other porcine viruses which are all associated frequently with similar clinical symptoms...
  13. ncbi In vitro and in vivo effects of Houttuynia cordata on infectious bronchitis virus
    Jiechao Yin
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030, Harbin, China
    Avian Pathol 40:491-8. 2011
    ..HC fully protected the SPF embryos, and had more than 50% protection rate in SPF chickens, against IBV challenge...
  14. ncbi Vaccination of mice with ORF5 plasmid DNA of PRRSV; enhanced effects by co-immunizing with porcine IL-15
    Guangxing Li
    College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
    Immunol Invest 41:231-48. 2012
    ..The pVAX-IL15 gene construct served as a molecular adjuvant in conjunction with the pVAX-GP5 to enhance the immune responses where intermediate doses of pVAX-IL15 were most effective...
  15. ncbi Cloning, prokaryotic expression, and biological analysis of recombinant chicken IFN-gamma
    Guangxing Li
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Hybridoma (Larchmt) 29:1-6. 2010
    ..Immunoreactivities of the CHIFN-gamma and its antiserum were investigated using immunoblotting and ELISA. Moreover, the antiviral effect of the CHIFN-gamma was analyzed. Our data indicate that the CHIFN-gamma is biologically active...
  16. ncbi Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18
    Siqingaowa Suo
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Virus Res 167:259-66. 2012
    ..In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-(IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1)...
  17. ncbi Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge
    Dexing Ma
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China
    Exp Parasitol 127:208-14. 2011
    ....
  18. ncbi Bacterial expression of antigenic sites A and D in the spike protein of transmissible gastroenteritis virus and evaluation of their inhibitory effects on viral infection
    Fandan Meng
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Virus Genes 43:335-41. 2011
    ..Furthermore, the truncated rS-AD peptide was able to bind to the surface of cells from swine testes in a competitive manner and completely inhibit viral infection...
  19. ncbi Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
    Virology 410:299-306. 2011
    ..Moreover, the excellent immune responses in mice induced by the identified phages provided the possibility to develop novel phage-based vaccines...
  20. ncbi Differentiation of porcine reproductive and respiratory syndrome virus N protein using a virus-based ELISA
    Guangxing Li
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Hybridoma (Larchmt) 30:195-8. 2011
    ..The polyclonal antibody against PRRSV N protein used as a diagnostic agent was capable of differentiating PRRSV from the other viruses...
  21. ncbi Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus
    Xiuwen Sui
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China
    Antiviral Res 85:346-53. 2010
    ..Virus plaque-reduction assays, PCR and RT-PCR analysis indicated that both drugs inhibited cell infection by PrV. Moreover, addition of the drugs resulted in fewer apoptotic cells during PrV infection...
  22. ncbi Comparative analysis of the effect of glycyrrhizin diammonium and lithium chloride on infectious bronchitis virus infection in vitro
    Jing Li
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Avian Pathol 38:215-21. 2009
    ..Our data indicate that GD and LiCl have potential to prevent IBV infection in vitro through different antiviral mechanisms. The data are helpful for using antivirals efficiently...
  23. ncbi Phages harboring specific peptides that recognize the N protein of the porcine reproductive and respiratory syndrome virus distinguish the virus from other viruses
    Xiaofeng Ren
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai St, Xiangfang District, 150030 Harbin, China
    J Clin Microbiol 48:1875-81. 2010
    ..More importantly, the selected phages harboring specific peptides that recognize the N protein of PRRSV were able to efficiently distinguish PRRSV from other viruses in enzyme-linked immunosorbent assays...
  24. ncbi Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08
    Mingcui Wang
    College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China
    J Vet Sci 10:309-15. 2009
    ....
  25. ncbi Generation and characterization of antibody against porcine interleukin-18
    Siqingaowa Suo
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    Hybridoma (Larchmt) 30:169-73. 2011
    ..Moreover, the pIL-18 was able to stimulate the proliferation of pig peripheral blood mononuclear cells in vitro, indicating it may have certain biological activity...
  26. ncbi Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system
    Boqi Liu
    College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    J Biotechnol 141:91-6. 2009
    ..The pAPN and its specific antibody blocked transmissible gastroenteritis coronavirus infection in vitro. Furthermore, the localization of pAPN on the small intestine of swine was analyzed by immunohistochemistry...
  27. ncbi The effect of Houttuynia cordata injection on pseudorabies herpesvirus (PrV) infection in vitro
    Xiaofeng Ren
    Department of Preventive Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Pharm Biol 49:161-6. 2011
    ..Houttuynia cordata Thunb. (Saururaceae), H. cordata, a traditional Chinese medicine, is often used to relieve lung abnormal symptoms, infectious disease, refractory hemoptysis and malignant pleural effusion in China...
  28. ncbi Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing spike genes from porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus
    Fandan Meng
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 8:e57468. 2013
    ..At 35 days post-immunization, the recombinant DNA plasmids bearing full-length S genes of TGEV and PEDV stimulated higher levels of specific antibodies and neutralizing antibodies in immunized mice...
  29. ncbi Expression, antibody generation, and biological analysis of chicken interleukin-18
    Heng Zhang
    College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    Hybridoma (Larchmt) 29:525-9. 2010
    ..coli or ChIL-18-transfected eukaryotic cells. Moreover, the renatured ChIL-18 stimulated the production of nitric oxide (NO) from macrophages via eliciting the secreting of IFN-γ from lymphocytes...
  30. ncbi Antibody against biologically active p40 subunit of porcine interleukin-12 expressed in Escherichia coli
    Xunliang Li
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    Hybridoma (Larchmt) 29:489-94. 2010
    ..Expression of p40 subunit on the surface of the transfected cells was identified using the anti-p40 antibody. The p40 protein and the specific antibody are biologically active and can be used as detecting reagents...
  31. ncbi Phages bearing affinity peptides to bovine rotavirus differentiate the virus from other viruses
    Xin Wang
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    PLoS ONE 6:e28667. 2011
    ..1 µg/ml of samples. The high sensitivity, specificity and low cross-reactivity for the phage-based ELISA were confirmed in receiver operating characteristics (ROC) analysis...
  32. ncbi Cholesterol is important for a post-adsorption step in the entry process of transmissible gastroenteritis virus
    Jiechao Yin
    Northeast Agricultural University, Harbin, China
    Antiviral Res 88:311-6. 2010
    ..Cholesterol is important for a post-adsorption step, allowing membrane rearrangements that facilitate virus entry...
  33. ncbi Incidence rates and deaths of tuberculosis in HIV-negative patients in the United States and Germany as analyzed by new predictive model for infection
    Yudong Ren
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 7:e42055. 2012
    ....
  34. ncbi Genome sequence of Chinese porcine parvovirus strain PPV2010
    Jin Cui
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    J Virol 86:2379. 2012
    ..Our results indicate that the genome of PPV2010 bears mixed characteristics of virulent PPV and vaccine strains. Importantly, PPV2010 has the potential to be a naturally attenuated candidate vaccine strain...
  35. ncbi Prokaryotic expression of Stx1B subunit of Escherichia coli O157:H7 used to generate monoclonal antibody
    Guangxing Li
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Hybridoma (Larchmt) 29:283-9. 2010
    ..Therefore, the MAb generated in this study can be used as a specific reagent to investigate the pathogenesis mechanism and to develop a diagnostic method of EHEC infection in both humans and animals...
  36. ncbi Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes
    Jiechao Yin
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China
    J Biotechnol 127:335-47. 2007
    ..Here, we concisely review the most frequently used prokaryotic, yeast, insect and mammalian expression systems, as well as expression in eukaryote individuals. The merits and demerits of these systems are discussed...
  37. ncbi Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus
    Pengchong Li
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang, 150030, Harbin, China
    Curr Microbiol 62:1074-80. 2011
    ..The approach is suitable for detecting TGEV for field diagnostics or in less-equipped laboratories due to its convenience and simplicity...
  38. ncbi Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein
    Chao Wang
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Hybridoma (Larchmt) 29:511-6. 2010
    ..The SARS-CoV S1 protein and its antibody are valuable reagents for related studies...
  39. ncbi Phage displayed peptides to avian H5N1 virus distinguished the virus from other viruses
    Dan Wu
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 6:e23058. 2011
    ..More importantly, the selected phages bearing the specific peptides to H5N1 viruses were capable of differentiating this virus from other avian viruses in enzyme-linked immunosorbent assays...
  40. ncbi A Monoclonal Antibody Against Transmissible Gastroenteritis Virus Generated via Immunization of a DNA Plasmid Bearing TGEV S1 Gene
    Qiong Zhao
    1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    Monoclon Antib Immunodiagn Immunother 32:50-4. 2013
    ..The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner...
  41. ncbi Monoclonal antibody against membrane protein of transmissible gastroenteritis virus
    Xuejiao Sun
    1 College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China
    Monoclon Antib Immunodiagn Immunother 32:36-40. 2013
    ..Immunofluorescence assays indicated that MAb 7G7 was capable of detecting cell infection by TGEV. Virus-based ELISA demonstrated that MAb 7G7 can be used as a highly specific diagnostic reagent for TGEV...
  42. ncbi Assembly of pseudorabies virus genome-based transfer vehicle carrying major antigen sites of S gene of transmissible gastroenteritis virus: potential perspective for developing live vector vaccines
    Jiechao Yin
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, PR China
    Biologicals 35:55-61. 2007
    ..Subsequently, a PRV genome-based virus live vector was generated via homologous recombination. The functionally effective vector was purified and partially characterized. Moreover, the potential advantages of this system are discussed...
  43. ncbi Use of the D-R model to define trends in the emergence of Ceftazidime-resistant Escherichia coli in China
    Fan Ding
    College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
    PLoS ONE 6:e27295. 2011
    ..To assess the efficacy of the D-R model for defining trends in the appearance of Ceftazidime-resistant Escherichia coli...
  44. ncbi Phylogeny and homologous recombination in Chikungunya viruses
    Jin Cui
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China
    Infect Genet Evol 11:1957-63. 2011
    ..These data refine the phylogeny of the currently available CHIKVs and homologous recombination did not contribute to the genetic diversity of these viruses...