Luo Z, Wang S, Jiao B, Yuan D, Dai D, Wang L, et al. Gene cloning and seamless site-directed mutagenesis using single-strand annealing (SSA). Appl Microbiol Biotechnol. 2018;: pubmed publisher
..In summary, our study provided an alternative method to overcome the difficulties during overlap PCR in some particular cases for gene cloning. ..
Shao S, Ren C, Liu Z, Bai Y, Chen Z, Wei Z, et al. Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017;92:43-52 pubmed publisher
..2-fold increase in HDR frequency. In conclusion, our data suggests that Rad52-Cas9 fusion is a good option for enhancing CRISPR/Cas9-mediated HDR, which may be of use in future studies involving precise genome editing. ..