Affiliation: Optical-Electrical Research Center of University of Shanghai for Science and Technology
Location: Shanghai, China
Li Z, Dou X, Ni Y, Yamaguchi Y. Separation of long DNA fragments by inversion field capillary electrophoresis. Anal Bioanal Chem. 2011;401:1661-7 pubmed publisher
..The inversion field capillary electrophoresis reported here may help enable future DNA analysis studies to be performed quickly and effectively. ..
Li Z, Dou X, Ni Y, Sumitomo K, Yamaguchi Y. The influence of polymer concentration, applied voltage, modulation depth and pulse frequency on DNA separation by pulsed field CE. J Sep Sci. 2010;33:2811-7 pubmed publisher
..Thus, the intermediate modulation depth (100%) and pulse frequency (about 31.3 Hz) are prerequisite for high-resolution DNA separation. ..
Li Z, Dou X, Ni Y, Sumitomo K, Yamaguchi Y. Acetic acid denaturing pulsed field capillary electrophoresis for RNA separation. Electrophoresis. 2010;31:3531-6 pubmed publisher
..5?Hz is the resonance frequency for RNA reorientation time and applied frequency. ..
Li Z, Dou X, Ni Y, Chen Q, Cheng S, Yamaguchi Y. Is pulsed electric field still effective for RNA separation in capillary electrophoresis?. J Chromatogr A. 2012;1229:274-9 pubmed publisher
..While these initial experiments show no distinct advantages of using PFCE for RNA separation, they do take further step toward characterizing the migration behavior of RNA under pulsed field conditions. ..
Li Z, Liu C, Zhang D, Luo S, Yamaguchi Y. Capillary electrophoresis of RNA in hydroxyethylcellulose polymer with various molecular weights. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1011:114-20 pubmed publisher
..Such a systematic investigation of long RNAs may be useful for research on lncRNAs in the length range of 100-10,000 nt. ..
Zhu Y, Li Z, Wang P, Shen L, Zhang D, Yamaguchi Y. Factors affecting the separation performance of proteins in capillary electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1083:63-67 pubmed publisher
..Finally, we employed the optimal electrophoretic conditions to resolve Lysozyme, Ovalbumin, BSA and their mixtures, and found that they were baseline resolved within 15â€¯min. ..
Tao C, Yang B, Li Z, Zhang D, Yamaguchi Y. Real-time Tracking of DNA Fragment Separation by Smartphone. J Vis Exp. 2017;: pubmed publisher
..The results demonstrate that a DNA ladder smaller than 5,000 bp can be resolved within 12 min and with high resolution when using this method, indicating that it is an ideal substitute for the traditional SGE method. ..