Shaoqun Zeng

Summary

Affiliation: Huazhong University of Science and Technology
Country: China

Publications

  1. doi request reprint Localization-based super-resolution microscopy with an sCMOS camera
    Zhen Li Huang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Express 19:19156-68. 2011
  2. ncbi request reprint Analysis of the dispersion compensation of acousto-optic deflectors used for multiphoton imaging
    Shaoqun Zeng
    Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics, Ministry of Education, Wuhan National Laboratory for Optoelectronics, Wuhan 430074 China
    J Biomed Opt 12:024015. 2007
  3. ncbi request reprint Acousto-optic modulator system for femtosecond laser pulses
    Shaoqun Zeng
    Wuhan National Laboratory for Optoelectronics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, People s Republic of China
    Rev Sci Instrum 78:015103. 2007
  4. ncbi request reprint Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism
    Shaoqun Zeng
    National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 31:1091-3. 2006
  5. doi request reprint Activity-dependent neuronal control of gap-junctional communication in fibroblasts
    Yan Zeng
    Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, HUST, Wuhan, China
    Brain Res 1280:13-22. 2009
  6. ncbi request reprint Position of the prism in a dispersion-compensated acousto-optic deflector for multiphoton imaging
    Kun Bi
    Wuhan National Laboratory for Opto electronics, Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China
    Appl Opt 45:8560-5. 2006
  7. doi request reprint Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device
    Dongli Xu
    Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    J Biomed Opt 18:060503. 2013
  8. doi request reprint Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography
    Ting Zheng
    Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    Opt Express 21:9839-50. 2013
  9. doi request reprint A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions
    Jun Chu
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, PR China
    Biosens Bioelectron 25:234-9. 2009
  10. ncbi request reprint A generalized analysis of femtosecond laser pulse broadening after angular dispersion
    Derong Li
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P R China
    Opt Express 16:237-47. 2008

Collaborators

Detail Information

Publications75

  1. doi request reprint Localization-based super-resolution microscopy with an sCMOS camera
    Zhen Li Huang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Express 19:19156-68. 2011
    ..We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region...
  2. ncbi request reprint Analysis of the dispersion compensation of acousto-optic deflectors used for multiphoton imaging
    Shaoqun Zeng
    Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics, Ministry of Education, Wuhan National Laboratory for Optoelectronics, Wuhan 430074 China
    J Biomed Opt 12:024015. 2007
    ..Fluorescence images of beads and cells are shown to demonstrate the performance of two-photon microscopy when applying this compensated 2-D AOD as scanner...
  3. ncbi request reprint Acousto-optic modulator system for femtosecond laser pulses
    Shaoqun Zeng
    Wuhan National Laboratory for Optoelectronics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, People s Republic of China
    Rev Sci Instrum 78:015103. 2007
    ..The fluorescence intensity in a two-photon microscopy experiment performed using this system increased 5.5-fold over that of the uncompensated AOM...
  4. ncbi request reprint Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism
    Shaoqun Zeng
    National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 31:1091-3. 2006
    ..14 with compensation. The insertion loss was 37%. Two-photon fluorescence microscopy used the compensated 2D AOD scanner to image a fluorescent microsphere, which improves signal intensity -15-fold compared with the uncompensated scanner...
  5. doi request reprint Activity-dependent neuronal control of gap-junctional communication in fibroblasts
    Yan Zeng
    Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, HUST, Wuhan, China
    Brain Res 1280:13-22. 2009
    ..Since fibroblasts have been shown to facilitate DRG neurite growth and survival, our findings provide a glimpse at the impact that neuronal activation has on fibroblast networks...
  6. ncbi request reprint Position of the prism in a dispersion-compensated acousto-optic deflector for multiphoton imaging
    Kun Bi
    Wuhan National Laboratory for Opto electronics, Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China
    Appl Opt 45:8560-5. 2006
    ..On the basis of these experiments, the prism-AOD configuration is concluded to be more advantageous for the purpose of simultaneous compensation for the spatial and temporal dispersion...
  7. doi request reprint Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device
    Dongli Xu
    Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    J Biomed Opt 18:060503. 2013
    ..9×10(7)  pixel/s of our system, an order of magnitude faster than previously reported...
  8. doi request reprint Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography
    Ting Zheng
    Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    Opt Express 21:9839-50. 2013
    ..Besides brain imaging, 2p-fMOST could be used in many studies that requires sub-micro resolution or micro resolution imaging of a large sample...
  9. doi request reprint A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions
    Jun Chu
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, PR China
    Biosens Bioelectron 25:234-9. 2009
    ..The mLumin-based BiFC system will facilitate simultaneous visualization of multiple protein-protein interactions in living cells and offer the potential to visualize protein-protein interactions in living animals...
  10. ncbi request reprint A generalized analysis of femtosecond laser pulse broadening after angular dispersion
    Derong Li
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P R China
    Opt Express 16:237-47. 2008
    ..The width of a plane wave pulse always increases in proportion to the propagation distance. The width of a spherical wave or Gaussian beam pulse widens rapidly at first, but soon levels off to nearly constant value...
  11. doi request reprint Characterizing the complexity of spontaneous electrical signals in cultured neuronal networks using approximate entropy
    Lin Chen
    BrittonChanceCenter forBiomedical Photonics, HuazhongUniversity of Science and Technology, Wuhan 430074, China
    IEEE Trans Inf Technol Biomed 13:405-10. 2009
    ..This suggests that the proposed method is a valid procedure for tracking the dynamic variation in neuronal signals and can distinguish the different firing patterns of neuronal networks in terms of their complexity...
  12. pmc Modeling the contributions of Ca2+ flows to spontaneous Ca2+ oscillations and cortical spreading depression-triggered Ca2+ waves in astrocyte networks
    Bing Li
    Britton Chance Center of Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, People s Republic of China
    PLoS ONE 7:e48534. 2012
    ..Research on the different types of Ca(2+) signals might help to understand the ways by which astrocytes participate in information processing in brain functions...
  13. doi request reprint Ultra-fast, high-precision image analysis for localization-based super resolution microscopy
    Tingwei Quan
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, PR China
    Opt Express 18:11867-76. 2010
    ..This newly developed method is also capable of revealing movements from the images immediately after data acquisition, which is of great benefit to live cell imaging...
  14. ncbi request reprint Visualization of beta-secretase cleavage in living cells using a genetically encoded surface-displayed [corrected] FRET probe
    Jinling Lu
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Biochem Biophys Res Commun 362:25-30. 2007
    ..The results show that the FRET probe can be cleaved by BACE effectively in vivo, suggesting that the probe can be used for real-time monitoring of BACE activity. This assay provides a novel platform for BACE inhibitor screening in vivo...
  15. ncbi request reprint Hyperosmotic chemical agent's effect on in vivo cerebral blood flow revealed by laser speckle
    Haiying Cheng
    The Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
    Appl Opt 43:5772-7. 2004
    ..The challenges for the design of an optical clearing technique were not only the clearing effects and the duration of the action of the chemical agents but also the influence of the glycerol on the tissue's normal physiological function...
  16. doi request reprint Micro-optical sectioning tomography to obtain a high-resolution atlas of the mouse brain
    Anan Li
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, P R China
    Science 330:1404-8. 2010
    ..The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other...
  17. pmc Method to reconstruct neuronal action potential train from two-photon calcium imaging
    Tingwei Quan
    Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    J Biomed Opt 15:066002. 2010
    ..Meanwhile, the APs firing at a high frequency up to 40 Hz can also be successfully identified. From the results, it can be concluded that the method has strong power to reconstruct a neuronal AP train from a burst firing...
  18. ncbi request reprint Numerical study of the effects of scatterer sizes and distributions on multiple backscattered intensity patterns of polarized light
    Yong Deng
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 33:77-9. 2008
    ..The results show that the polarization patterns of dysplastic and normal cells have distinct differences, which might be used for identification of the morphological structure changes of cancer, dysplasia, and regeneration cells...
  19. doi request reprint Cortical spreading depression in rats
    Shangbin Chen
    Huazhong University of Science and Technology, Wuhan 430074, China
    IEEE Eng Med Biol Mag 27:29-35. 2008
  20. doi request reprint Characteristics of calcium signaling in astrocytes induced by photostimulation with femtosecond laser
    Yuan Zhao
    Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, China
    J Biomed Opt 15:035001. 2010
    ..Therefore, distinct Ca(2+) signaling is feasibly available for specific studies on astrocytes by employing precisely controlled photostimulation...
  21. ncbi request reprint Origin sites of spontaneous cortical spreading depression migrated during focal cerebral ischemia in rats
    Shangbin Chen
    The Key Laboratory of Biomedical Photonics of Ministry of Education Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Neurosci Lett 403:266-70. 2006
    ..Hence, the determination of origins of spontaneous CSD using OISI would contribute to the continued study of stroke...
  22. doi request reprint Localization-based super-resolution microscopy with an sCMOS camera part II: experimental methodology for comparing sCMOS with EMCCD cameras
    Fan Long
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Express 20:17741-59. 2012
    ....
  23. ncbi request reprint Time-varying spreading depression waves in rat cortex revealed by optical intrinsic signal imaging
    Shangbin Chen
    The Key Laboratory of Biomedical Photonics of Ministry of Education Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Neurosci Lett 396:132-6. 2006
    ..2+/-29.0 s). The results suggest that the propagation patterns of a series of SD waves are time-varying in different regions of rat cortex, and the variation is related to the interval between SD waves...
  24. ncbi request reprint Imaging cerebral blood flow through the intact rat skull with temporal laser speckle imaging
    Pengcheng Li
    Key Laboratory of Biomedical Photonics of Ministry of Education Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 31:1824-6. 2006
    ....
  25. ncbi request reprint TRAIL-induced apoptosis proceeding from caspase-3-dependent and -independent pathways in distinct HeLa cells
    Juqiang Lin
    The Key Laboratory of Biomedical Photonics of Ministry of Education, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Biochem Biophys Res Commun 346:1136-41. 2006
    ..The observation of the coexistence of caspase-3-dependent and caspase-3-independent apoptotic pathways in the TRAIL-treated cells was unusual in comparison with the previous reports...
  26. ncbi request reprint Characterization of synchronized bursts in cultured hippocampal neuronal networks with learning training on microelectrode arrays
    Yanling Li
    The Key Laboratory of Biomedical Photonics of Ministry of Education Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Biosens Bioelectron 22:2976-82. 2007
    ..Furthermore, from these observations, we are encouraged to think of a more engineered system based on in vitro hippocampal neurons, as a novel sensitive system for electrophysiological evaluations...
  27. ncbi request reprint Differentiating hemodynamic responses in rat primary somatosensory cortex during non-noxious and noxious electrical stimulation by optical imaging
    Weihua Luo
    Key Laboratory of Biomedical Photonics of Ministry of Education Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Brain Res 1133:67-77. 2007
    ..The magnitude-dependent inverted optical signal might contribute to the differentiation of nociceptive input in the S1 cortex...
  28. pmc Dynamics of learning in cultured neuronal networks with antagonists of glutamate receptors
    Yanling Li
    The Key Laboratory of Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, People s Republic of China
    Biophys J 93:4151-8. 2007
    ....
  29. pmc NeuroGPS: automated localization of neurons for brain circuits using L1 minimization model
    Tingwei Quan
    Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    Sci Rep 3:1414. 2013
    ..This method allows locating the neurons across different brain areas without human intervention. We believe this method would facilitate the analysis of the neuronal circuits for brain function and disease studies...
  30. doi request reprint Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection
    Xiaoli Qi
    Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan 430074, China
    J Biomed Opt 18:50506. 2013
    ....
  31. doi request reprint Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors
    Yafeng Liu
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    Rev Sci Instrum 83:025116. 2012
    ..The compact and modular design of this photostimulator makes it easily integrated with different commercial microscopes, and thus widely popularized in many laboratories...
  32. doi request reprint Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging
    Tingwei Quan
    Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    J Biomed Opt 15:066005. 2010
    ....
  33. ncbi request reprint A modified mini-stroke model with region-directed reperfusion in rat cortex
    Weihua Luo
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, PR China
    J Cereb Blood Flow Metab 28:973-83. 2008
    ..Our data suggest that this model provides opportunities for investigating the neurovascular dynamics in acute stroke and rehabilitation, especially with emerging optical imaging techniques...
  34. doi request reprint Activation of caspase-3 noninvolved in the bystander effect of the herpes simplex virus thymidine kinase gene/ganciclovir (HSV-tk/GCV) system
    Zhihong Zhang
    Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, China
    J Biomed Opt 13:031209. 2008
    ....
  35. doi request reprint Fluorescence imaging to assess the matrix metalloproteinase activity and its inhibitor in vivo
    Zhihong Zhang
    Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    J Biomed Opt 13:011006. 2008
    ..This proves a novel method to efficiently screen and assess the anticancer drug MMP inhibitor in living cells and in vivo tumor models...
  36. ncbi request reprint In vivo optical reflectance imaging of spreading depression waves in rat brain with and without focal cerebral ischemia
    Shangbin Chen
    Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Wuhan, China
    J Biomed Opt 11:34002. 2006
    ..The penumbra appear bright due to a rapid drop in perfusion, while the normal brain and infarct area appear dark...
  37. ncbi request reprint Propagation dependence of chirp in Gaussian pulses and beams due to angular dispersion
    Derong Li
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
    Opt Lett 34:962-4. 2009
    ..This work verifies both an equation and a measurement technique that will be useful for predicting or determining the pulse's chirp in ultrafast optics experiments that contain angular dispersion...
  38. doi request reprint Tetrameric far-red fluorescent protein as a scaffold to assemble an octavalent peptide nanoprobe for enhanced tumor targeting and intracellular uptake in vivo
    Haiming Luo
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
    FASEB J 25:1865-73. 2011
    ..Owing to easy preparation, precise structural and functional control, and multivalent effect, Octa-FNP provides a powerful tool for tumor optical molecular imaging and evaluating the targeting ability of numerous peptides in vivo...
  39. doi request reprint Localization-based super-resolution microscopy with an sCMOS camera part III: camera embedded data processing significantly reduces the challenges of massive data handling
    Hongqiang Ma
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 38:1769-71. 2013
    ..2%) at molecular densities <0.2 molecules/μm(2), thus significantly reducing the challenges of data transfer, storage, and analysis in LM...
  40. pmc Rapid Reconstruction of 3D Neuronal Morphology from Light Microscopy Images with Augmented Rayburst Sampling
    Xing Ming
    Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, Wuhan, China MoE Key Laboratory of Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, China
    PLoS ONE 8:e84557. 2013
    ..The results showed that the system achieves a reasonable balance between fast speed and acceptable accuracy, which is promising for interactive applications in neuronal image analysis. ..
  41. ncbi request reprint Detection of MMP activity in living cells by a genetically encoded surface-displayed FRET sensor
    Jie Yang
    The Key Laboratory of Biomedical Photonics of Ministry of Education, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Biochim Biophys Acta 1773:400-7. 2007
    ..The FRET sensor YFP-MSS-CFPdisplay can sensitively and reliably monitor MMP activation in living cells and can be used for high-throughput screening of MMP inhibitors for anti-cancer treatments...
  42. doi request reprint Continuously tracing brain-wide long-distance axonal projections in mice at a one-micron voxel resolution
    Hui Gong
    Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China
    Neuroimage 74:87-98. 2013
    ..With advancing labeling techniques, our method is believed to open an avenue to exploring both local and long-distance neural circuits that are related to brain functions and brain diseases down to the neurite level...
  43. doi request reprint Super-resolution differential interference contrast microscopy by structured illumination
    Jianling Chen
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Express 21:112-21. 2013
    ..The SI-DIC microscopy can provide sub-diffraction resolution and high contrast images with marker-free specimens, and has the potential for achieving sub-diffraction resolution quantitative phase imaging...
  44. ncbi request reprint Spatiotemporal characteristics of cerebral blood volume changes in rat somatosensory cortex evoked by sciatic nerve stimulation and obtained by optical imaging
    Pengcheng Li
    Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of the Ministry of Education, Wuhan, 430074, Hubei, People s Republic of China
    J Biomed Opt 8:629-35. 2003
    ....
  45. ncbi request reprint Laser speckle imaging of blood flow in microcirculation
    Haiying Cheng
    The Key Laboratory of Biomedical Photonics of Ministry of Education of China, Huazhong University of Science and Technology, Wuhan 430074, People s Republic of China
    Phys Med Biol 49:1347-57. 2004
    ..At the same time, different responses between veinules and arterioles were manifested. These suggested that LSI is promising to be a useful contribution to drug development and testing...
  46. ncbi request reprint Photostimulation of astrocytes with femtosecond laser pulses
    Yuan Zhao
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, China
    Opt Express 17:1291-8. 2009
    ..Photostimulation of astrocytes would facilitate investigations on information processing in neuronal circuits by providing effective way to excite astrocytes...
  47. doi request reprint Organ dose calculations by Monte Carlo modeling of the updated VCH adult male phantom against idealized external proton exposure
    Guozhi Zhang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, People s Republic of China
    Phys Med Biol 53:3697-722. 2008
    ..This work pertains to VCH-based systematic multi-particle dose investigations and will contribute to comparative dosimetry studies of ICRP standardized voxel phantoms in the near future...
  48. doi request reprint High-density localization of active molecules using Structured Sparse Model and Bayesian Information Criterion
    Tingwei Quan
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
    Opt Express 19:16963-74. 2011
    ..We show that the SSM_BIC is superior in processing single molecule images with weak signal embedded in strong background...
  49. pmc mBeRFP, an improved large stokes shift red fluorescent protein
    Jie Yang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, China
    PLoS ONE 8:e64849. 2013
    ..Thus, mBeRFP appears to be particularly useful for cellular imaging applications. ..
  50. doi request reprint PALMER: a method capable of parallel localization of multiple emitters for high-density localization microscopy
    Yina Wang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Express 20:16039-49. 2012
    ....
  51. doi request reprint Wide-band acousto-optic deflectors for large field of view two-photon microscope
    Runhua Jiang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China
    Rev Sci Instrum 83:043709. 2012
    ..58-2.12 μm and 2.17-3.07 μm, respectively. Pollen grain images acquired by this system were presented to demonstrate the imaging capability at different positions across the entire FOV...
  52. doi request reprint Spatial and temporal thermal analysis of acousto-optic deflectors using finite element analysis model
    Runhua Jiang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    Ultrasonics 52:643-9. 2012
    ..The effect of heat source and heat convection on temperature distribution is discussed. This numerical model and analytical method of thermal analysis would be helpful in the thermal design and practical applications of AODs...
  53. doi request reprint The development and application of the visible Chinese human model for Monte Carlo dose calculations
    Guozhi Zhang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, PR China
    Health Phys 94:118-25. 2008
    ..Detailed information from the VCH model is able to improve the radiological datasets, particular for the Chinese population, and provide insights into the research of various computational phantoms...
  54. doi request reprint Adaptive region of interest method for analytical micro-CT reconstruction
    Wanneng Yang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan, China
    J Xray Sci Technol 19:23-33. 2011
    ..2 times with AROI and the imaging quality is not degraded. Therefore, the AROI method improves the speed of analytical micro-CT reconstruction significantly...
  55. pmc Simulation of spontaneous Ca2+ oscillations in astrocytes mediated by voltage-gated calcium channels
    Shuai Zeng
    Britton Chance Center of Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan, China
    Biophys J 97:2429-37. 2009
    ..Therefore, calcium channels dynamics should be used in studying Ca(2+) oscillations. This work provides a platform to explore the still unclear mechanism of spontaneous Ca(2+) oscillations in astrocytes...
  56. doi request reprint Violation of the Lagrange invariant in an optical imaging system
    Xiaomin Lai
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China
    Opt Lett 38:1896-8. 2013
    ..With a proof-of-principle system, we proved this violation both theoretically and experimentally. This finding enables future exciting possibilities in optical imaging...
  57. doi request reprint Fast and precise algorithm based on maximum radial symmetry for single molecule localization
    Hongqiang Ma
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    Opt Lett 37:2481-3. 2012
    ....
  58. doi request reprint Confocal imaging reveals three-dimensional fine structure difference between ventral and dorsal nerve roots
    Yuxiang Wu
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
    J Biomed Opt 16:050502. 2011
    ..These structural differences could help surgeons to differentiate ventral and dorsal nerves in peripheral nerve injury repair, and also facilitate scientists to get a deeper understanding about nerve fiber organization...
  59. ncbi request reprint Identification and two-photon imaging of oligodendrocyte in CA1 region of hippocampal slices
    Wei Zhou
    The Key Laboratory of Biomedical Photonics of Ministry of Education Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
    Biochem Biophys Res Commun 352:598-602. 2007
    ..When stimulating Schaffer fiber, OL displays a long duration depolarization mediated by inward rectifier potassium channel. This suggested that the OL in CA1 region could sense the neuronal activity and contribute to potassium clearance...
  60. ncbi request reprint Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis
    Juqiang Lin
    Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of Ministry of Education Hubei, Bioinformatics and Molecular Imaging Key Laboratory, Wuhan 430074 China
    J Biomed Opt 11:024011. 2006
    ..Using these probes, we found that during cisplatin-induced apoptosis, caspase-2 activation occurred more slowly than did activation of caspase-3; additionally, caspase-2 activation was initiated much earlier than that of caspase-3...
  61. ncbi request reprint Effect of light losses of sample between two integrating spheres on optical properties estimation
    Dan Zhu
    Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China
    J Biomed Opt 12:064004. 2007
    ..This study can not only evaluate the error of optical properties estimation, but also provide optimal ways for the design of DIS and a scheme for acquiring accurate optical properties of tissue...
  62. ncbi request reprint Using running subtraction to detect the wavefront of cortical spreading depression
    Shangbin Chen
    Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, P R China E mail
    Conf Proc IEEE Eng Med Biol Soc 2:1446-8. 2005
    ..e. wavefront), and the extracted spatial pattern of CSD showed less vascular artifacts. For these advantages, running subtraction proved to be an appropriate approach to detect the wavefront of CSD...
  63. ncbi request reprint Long-term recording on multi-electrode array reveals degraded inhibitory connection in neuronal network development
    Xiangning Li
    The Key Laboratory of Biomedical Photonics of Ministry of Education Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China
    Biosens Bioelectron 22:1538-43. 2007
    ..Long-term cultures on MEA provided a useful tool to measure the relationship between spontaneous developmental change and pharmacological influence in vitro...
  64. doi request reprint High-throughput measurement of rice tillers using a conveyor equipped with x-ray computed tomography
    Wanneng Yang
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
    Rev Sci Instrum 82:025102. 2011
    ..This facility expands the application of agricultural photonics in plant phenomics...
  65. doi request reprint Fluorescence holography with improved signal-to-noise ratio by near image plane recording
    Xiaomin Lai
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China
    Opt Lett 37:2445-7. 2012
    ..With experiments, we demonstrate an example of an increase of about 21 times in SNR by changing f(2) from infinity to 226 mm, which is close to f(1) (323 mm)...
  66. ncbi request reprint Whole-body Fluorescent Optical Imaging Based on Power Light Emitting Diode
    Yanping Chen
    Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, P R China E mail
    Conf Proc IEEE Eng Med Biol Soc 2:1442-5. 2005
    ..This study puts forward a platform for monitoring tumor growth. The experiment reveals that it is doable to substitute power LED for mercury lamp for whole-body fluorescence optical imaging...
  67. ncbi request reprint The origin of spontaneous synchronized burst in cultured neuronal networks based on multi-electrode arrays
    Chuanping Chen
    Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China
    Biosystems 85:137-43. 2006
    ..In a word, the generation of the burst requires that network should have a sufficient level of excitation as well as a balance of synaptic inhibition...
  68. ncbi request reprint Identification of the direction of the neural network activation with a cellular resolution by fast two-photon imaging
    Xiuli Liu
    Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinaHuazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of Ministry of Education, Wuhan 430074, ChinaHubei University of Education, College of Mathematics and Economics, Wuhan 430205, China
    J Biomed Opt 16:080506. 2011
    ..As an example, we characterized a directional neuronal network in an epilepsy brain slice to provide different initiation delay among multiple neurons defined at a millisecond scale...
  69. ncbi request reprint L-type calcium channels mediate synchronized spontaneous Ca<sup>2+</sup>spikes in cultured cortical networks
    Jun He
    Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, P R China E mail
    Conf Proc IEEE Eng Med Biol Soc 2:1783-5. 2005
    ..Ca<sup>2+</sup>entry occurred primarily through L-type voltage-gated calcium channels (VGCCs), since nifedipine completely blocked these Ca<sup>2+</sup>spikes...
  70. ncbi request reprint In vivo optical imaging of human adenoid cystic carcinoma cell metastasis
    Tao Xiong
    The Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China
    Oral Oncol 41:709-15. 2005
    ..The construction of highly fluorescent and stable GFP transfectants of ACC-M has revealed the multi-organ metastatic capability of ACC-M cells through this optical imaging...
  71. ncbi request reprint Efficient characterization of regional mesenteric blood flow by use of laser speckle imaging
    Haiying Cheng
    The Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
    Appl Opt 42:5759-64. 2003
    ..Spatial and temporal characteristics of the mesenteric blood-flow response were achieved with high resolution. These suggested that LSI might provide a new approach to microcirculation studies...
  72. pmc Dynamic analysis of optimality in myocardial energy metabolism under normal and ischemic conditions
    Ruo Yu Luo
    Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    Mol Syst Biol 2:2006.0031. 2006
    ..This finding supports the relevance of our hypothesis and could contribute to the further exploration of the underlying mechanism of dynamic regulation in metabolic networks...
  73. ncbi request reprint Modified laser speckle imaging method with improved spatial resolution
    Haiying Cheng
    Huazhong University of Science and Technology, The Key Laboratory of Biomedical Photonics of the Ministry of Education, Department of Biomedical Engineering, Wuhan, Hubei, China 430074
    J Biomed Opt 8:559-64. 2003
    ..These results suggest that this is a suitable method for imaging the full field of blood flow without scanning and provides much higher spatial resolution than that of current LASCA and other laser Doppler perfusion imaging methods...
  74. pmc In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits
    Shin Nagayama
    Department of Neurobiology, Yale University, New Haven, CT 06520 8001, USA
    Neuron 53:789-803. 2007
    ..This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits...
  75. ncbi request reprint Dendritic excitability and calcium signalling in the mitral cell distal glomerular tuft
    Zhishang Zhou
    Yale University Department of Neurobiology, 333 Cedar Street, SHM C303, New Haven, CT 06510, USA
    Eur J Neurosci 24:1623-32. 2006
    ..In summary, the mitral cell distal dendritic tuft possesses both Na+ and Ca2+ voltage-dependent conductances which can mediate glomerular Ca2+ responsiveness critical for dendrodendritic output and synaptic plasticity...