Chengyu Hu


Country: China


  1. Weng P, Wu L, Jiang Z, Ran X, Xu K, Xie X, et al. Grass carp (Ctenopharyngodon idella) RSK2 protects cells anti-apoptosis by up-regulating BCL-2. Dev Comp Immunol. 2019;97:45-56 pubmed publisher
    ..Then cell apoptosis was inhibited to a certain extent. Overall, our results suggested that CiRSK2 plays important roles in fish innate immunity and is able to inhibit cell apoptosis by up-regulating CiBCL-2. ..
  2. Xu X, Li M, Li D, Jiang Z, Liu C, Shi X, et al. Identification of the SAMHD1 gene in grass carp and its roles in inducing apoptosis and inhibiting GCRV proliferation. Fish Shellfish Immunol. 2019;: pubmed publisher
    ..Crystal violet staining, detection of two GCRV genes (vp3 and vp5) and viral titration showed that CiSAMHD1 can suppress the proliferation of grass carp reovirus (GCRV) in CIK cells. ..
  3. Wu C, Xu X, Zhi X, Jiang Z, Li Y, Xie X, et al. Identification and functional characterization of IRAK-4 in grass carp (Ctenopharyngodon idellus). Fish Shellfish Immunol. 2019;87:438-448 pubmed publisher
    ..In addition, IRAK4 promoted IRF5 nuclear translocation, which has nothing to do with the interaction between IRAK4 and IRF5. It suggested that fish IRAK4 kinase regulated IRF5 activity through indirect ways. ..
  4. Chen H, Sun C, Liu W, Gu M, Lin G, Liu Y, et al. Promoter analysis and transcriptional regulation of a Gig2 gene in grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2015;42:249-55 pubmed publisher
    ..ISRE2 is more important than ISRE1 and ISRE3 to the transcription of CiGig2 induced by CiIRF1. ISRE1 and ISRE3 are important to the transcription of CiGig2 induced by CiIRF2 and CiIRF3. ..
  5. Ran X, Liu C, Weng P, Xu X, Lin G, Qi G, et al. Activated grass carp STAT6 up-regulates the transcriptional level and expression of CCL20 and Bcl-xl. Fish Shellfish Immunol. 2018;80:214-222 pubmed publisher
    ..In addition, we also found that CiSTAT6 can increase cell viability and inhibit cell apoptosis. Taken together, grass carp STAT6 plays an important part in innate immunity and anti-apoptosis. ..
  6. Gu M, Lin G, Lai Q, Zhong B, Liu Y, Mi Y, et al. Ctenopharyngodon idella IRF2 plays an antagonistic role to IRF1 in transcriptional regulation of IFN and ISG genes. Dev Comp Immunol. 2015;49:103-12 pubmed publisher
    ..1-IRF2 and pcDNA3.1-IRF1) were transiently co-transfected with pGL3-IFN or pGL3-CiPKZ into CIK cells, respectively. The results revealed that CiIRF2 plays an antagonistic role to CiIRF1 in transcriptional regulation of IFN and ISG genes. ..
  7. Zhong B, Mao H, Fan Q, Liu Y, Hu Y, Mi Y, et al. SiRNA-mediated knockdown of CiGRP78 gene expression leads cell susceptibility to heavy metal cytotoxicity. Gene. 2014;552:219-24 pubmed publisher
    ..The viability rate of CIK cells transfected with or without siRNA incubated with HgCl2 for 12h showed a significant decrease from 50% to 21%. Our results showed that CiGRP78 protects cells against heavy metal stimuli to some extent. ..
  8. Yu N, Xu X, Qi G, Liu D, Chen X, Ran X, et al. Ctenopharyngodon idella TBK1 activates innate immune response via IRF7. Fish Shellfish Immunol. 2018;80:521-527 pubmed publisher
    ..Furthermore, grass carp TBK1 can promote the nuclear translocation of IRF7. The results indicated that grass carp TBK1 can bind directly to and activate IRF7. ..
  9. Li M, Xu X, Jiang Z, Liu C, Shi X, Qi G, et al. Fish SAMHD1 performs as an activator for IFN expression. Dev Comp Immunol. 2018;86:138-146 pubmed publisher
    ..Taken together, all these results suggest that grass carp SAMHD1 performs as an activator for innate immune response through STING-mediated signaling pathway. ..


More Information


  1. Hu Y, Fan L, Wu C, Wang B, Sun Z, Hu C. Identification and function analysis of the three dsRBMs in the N terminal dsRBD of grass carp (Ctenopharyngodon idella) PKR. Fish Shellfish Immunol. 2016;50:91-100 pubmed publisher
    ..In conclusion, our observations suggested that two dsRBMs ranking in tandem at N terminal were essential for the function of CiPKR, and the presence of the extra dsRBM1 enhanced its function. ..
  2. Liu X, Huang K, Hou Q, Sun Z, Wang B, Lin G, et al. Identification and characterization of a constitutively expressed Ctenopharyngodon idella ADAR1 splicing isoform (CiADAR1a). Dev Comp Immunol. 2016;63:10-7 pubmed publisher
    ..Together, according to the molecular and expression characteristics, subcellular localization and transcriptional regulatory mechanism, we deduced that CiADAR1a shared a high degree of homology with mammalian ADAR1-p110. ..
  3. Xu X, Lai Q, Gu M, Liu D, Hou Q, Liu X, et al. Fish IRF3 up-regulates the transcriptional level of IRF1, IRF2, IRF3 and IRF7 in CIK cells. Fish Shellfish Immunol. 2015;47:978-85 pubmed publisher
    ..Furthermore, overexpression of CiIRF3 in CIK cells also up-regulated the expressions of CiIRF1, CiIRF2, CiIRF3 and CiIRF7. So, CiIRF3 can improve the transcriptional level of CiIRF1, CiIRF2, CiIRF3 and CiIRF7. ..
  4. Wu C, Hu Y, Fan L, Wang H, Sun Z, Deng S, et al. Ctenopharyngodon idella PKZ facilitates cell apoptosis through phosphorylating eIF2α. Mol Immunol. 2016;69:13-23 pubmed publisher
    ..Overall, these results suggested that PKZ could facilitate apoptosis via eIF2α phosphorylation. ..
  5. Liu D, Mao H, Gu M, Xu X, Sun Z, Lin G, et al. The transcription regulation analysis of Ctenopharyngodon idellus PKR and PKZ genes. Gene. 2016;576:512-9 pubmed publisher
    ..The results also revealed that the consensus sequence of ISRE (interferon stimulated response element) is an important regulatory element for the transcriptional initiation of CiPKR and CiPKZ. ..
  6. Wang B, Liu Y, Sun Z, Liu X, Mi Y, Liu D, et al. A splicing isoform of Ctenopharyngodon idella ADAR1 (CiADAR1-like): Genome organization, tissue specific expression and transcriptional regulation. Fish Shellfish Immunol. 2015;47:535-44 pubmed publisher
    ..1-IRF1 (or pcDNA3.1-IRF3) with pGL3-CiADAR1-like promoter into CIK cells showed that both IRF1 and IRF3 significantly increased the luciferase activity, suggesting that they play a positive role in CiADAR1-like transcription. ..
  7. Wang X, Mi Y, Zhong B, Mao H, Wan Y, Zhang T, et al. Identification of grass carp (Ctenopharyngodon idella) XBP1S as a primary member in ER stress. Fish Shellfish Immunol. 2017;64:84-92 pubmed publisher
    ..1-CiXBP1S (pcDN3.1-CiXBP1S-nBRLZ, respectively) into EPC cells. The result showed that CiXBP1S can activate CiGRP78 and CiGRP94 promoters. ..
  8. Huang K, Qi G, Sun Z, Liu X, Xu X, Wang H, et al. Ctenopharyngodon idella IRF2 and ATF4 down-regulate the transcriptional level of PRKRA. Fish Shellfish Immunol. 2017;64:155-164 pubmed publisher
    ..1-CiIRF2 and pcDNA3.1-CiATF4, respectively. The results showed that both CiIRF2 and CiATF4 significantly decreased the luciferase activity of pGL3-PRKRAPro, suggesting that they play a negative role in CiPRKRA transcription. ..
  9. Wu Z, Wang L, Xu X, Lin G, Mao H, Ran X, et al. Interaction of IRF9 and STAT2 synergistically up-regulates IFN and PKR transcription in Ctenopharyngodon idella. Mol Immunol. 2017;85:273-282 pubmed publisher
    ..coli. A GST pull-down assay suggested that GST-tagged ST2-936 efficiently bound to FLAG-tagged IRF9. The data indicated that interaction of IRF9 and STAT2 synergistically up-regulated the transcriptional level of IFN and ISG genes. ..
  10. Zhong B, Wang X, Mao H, Wan Y, Liu Y, Zhang T, et al. A mechanism underlies fish GRP78 protection against Pb2+ toxicity. Fish Shellfish Immunol. 2017;66:185-188 pubmed publisher
    ..Our results suggested that CiGRP78 might increase cellular tolerance to Pb2+ via the direct interaction with it. ..
  11. Wang L, Wu Z, Huang Q, Huang K, Qi G, Wu C, et al. Grass carp (Ctenopharyngodon idella) STAT3 regulates the eIF2? phosphorylation through interaction with PKR. Dev Comp Immunol. 2018;78:26-34 pubmed publisher
    ..These results above unraveled the molecular mechanism of fish cytoplasmic STAT3 regulating the eIF2? phosphorylation and cell viability. Therefore, the function of fish cytoplasmic STAT3 is similar to those of mammals. ..
  12. Wang X, Zhang T, Mao H, Mi Y, Zhong B, Wei L, et al. Grass carp (Ctenopharyngodon idella) ATF6 (activating transcription factor 6) modulates the transcriptional level of GRP78 and GRP94 in CIK cells. Fish Shellfish Immunol. 2016;52:65-73 pubmed publisher
    ..1-CiATF6 (pcDN3.1-CiATF6-nBRLZ, respectively) into C. idella kidney (CIK) cells. The result has shown that CiATF6 could activate CiGRP78 and CiGRP94 promoters. ..
  13. Sun Z, Wang B, Liu Y, Liu X, Mi Y, Gu M, et al. Genome organization and transcriptional regulation of Adenosine Deaminase Acting on RNA gene 1 (ADAR1) in grass carp (Ctenopharyngodon idella). Dev Comp Immunol. 2015;50:98-105 pubmed publisher
    ..In addition, the mutant assay revealed that the proximal region of CiADAR1 promoter is the main regulatory region in CiADAR1 transcription. ..
  14. Wang H, Zhu Y, Xu X, Wang X, Hou Q, Xu Q, et al. Ctenopharyngodon idella NF-?B subunit p65 modulates the transcription of I?B? in CIK cells. Fish Shellfish Immunol. 2016;54:564-72 pubmed publisher
    ..1-Cip65-?C and pcDNA3.1-Cip65-?N, can increase the luciferase activity. Taken together, these results suggested that Cip65 can regulate the expression of CiI?B? and works as a negative feedback loop in NF-?B pathway. ..
  15. Hou Q, Gong R, Liu X, Mao H, Xu X, Liu D, et al. Poly I:C facilitates the phosphorylation of Ctenopharyngodon idellus type I IFN receptor subunits and JAK kinase. Fish Shellfish Immunol. 2017;60:13-20 pubmed publisher
    ..As a basic mechnism of feedback regulation of JAK-STAT signaling pathway, overexpression of CiCRFB1 and CiCRFB5 in CIK cells facilitated the phosphorylation of CiJAK1 and CiTYK2. ..
  16. Huang Q, Xie D, Mao H, Wang H, Wu Z, Huang K, et al. Ctenopharyngodon idella p53 mediates between NF-?B and PKR at the transcriptional level. Fish Shellfish Immunol. 2017;69:258-264 pubmed publisher
    ..When Cip53 was knockdown in CIK cells, the mRNA levels of Cip65 and CiPKR were decreased. Taken together, p53 may play pivotal roles in transcription pathway of NF-?B and PKR in fish. ..
  17. Zhang T, Li D, Wan L, Chen X, Wang X, Zhong B, et al. Ctenopharyngodon idella PERK (EIF2AK3) decreases cell viability by phosphorylating eIF2? under ER stress. Fish Shellfish Immunol. 2017;70:568-574 pubmed publisher
    ..However, under ER stress (TM stimulation) CiPERK can improve the eIF2? phosphorylation level. In addition, CCK assay showed the overexpression of CiPERK in CIK cells decreases the cell viability. ..
  18. Xu X, Li M, Wu Z, Wang H, Wang L, Huang K, et al. Endoplasmic Reticulum Transmembrane Proteins ZDHHC1 and STING Both Act as Direct Adaptors for IRF3 Activation in Teleost. J Immunol. 2017;199:3623-3633 pubmed publisher
    ..In conclusion, STING and ZDHHC1 in fish can respond to viral DNA or RNA molecules in cytoplasm, as well as activate IRF3 and, eventually, trigger IFN expression. ..
  19. Chen H, Liu W, Wang B, Mao H, Sun Z, Hou Q, et al. Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2015;45:211-20 pubmed publisher
    ..This indicated that the homodimer and the heterodimer were the potential receptor for CiIFN. ..