Huawei Li

Summary

Affiliation: Fudan University
Country: China

Publications

  1. pmc Identification and functional study of a new missense mutation in the motor head domain of myosin VIIA in a family with autosomal dominant hearing impairment (DFNA11)
    Qing Sang
    State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, People s Republic of China
    PLoS ONE 8:e55178. 2013
  2. pmc Differentiation of neurons from neural precursors generated in floating spheres from embryonic stem cells
    Huawei Li
    Department of Otolarygology, EENT Hospital of Shanghai Medical College of Fudan University, Institute of Biomedical Sciences, Fudan University Shanghai, 200031, PR China
    BMC Neurosci 10:122. 2009
  3. pmc BMP4 signaling is involved in the generation of inner ear sensory epithelia
    Huawei Li
    Department of Otolaryngology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
    BMC Dev Biol 5:16. 2005
  4. ncbi request reprint Characterization of proliferating cells from newborn mouse cochleae
    Zhengmin Wang
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, PR China
    Neuroreport 17:767-71. 2006
  5. ncbi request reprint Conditioned medium from neonatal rat olfactory ensheathing cells promotes the survival and proliferation of spiral ganglion cells
    Hongmeng Yu
    Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai
    Acta Otolaryngol 130:351-7. 2010
  6. ncbi request reprint Islet-1 expression in the developing chicken inner ear
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School, and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    J Comp Neurol 477:1-10. 2004
  7. pmc Engraftment and differentiation of embryonic stem cell-derived neural progenitor cells in the cochlear nerve trunk: growth of processes into the organ of Corti
    C Eduardo Corrales
    Department of Otology and Laryngology, Harvard Medical School, Boston, MA 02115, USA
    J Neurobiol 66:1489-500. 2006
  8. pmc Cotransfection of Pax2 and Math1 promote in situ cochlear hair cell regeneration after neomycin insult
    Yan Chen
    1 Otology Skull base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University Shanghai, 200031, P R China 2 Central laboratory, Eye and ENT Hospital of Shanghai Medical School, Fudan University Shanghai, 200031, P R China 3
    Sci Rep 3:2996. 2013
  9. doi request reprint A novel mutation in the MITF may be digenic with GJB2 mutations in a large Chinese family of Waardenburg syndrome type II
    Xukun Yan
    Department of Otolaryngology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China
    J Genet Genomics 38:585-91. 2011
  10. ncbi request reprint Peripherin as a marker for degeneration of spiral ganglion neurons after aminoglycoside ototoxicity
    Yucheng Wang
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai
    Acta Otolaryngol 126:1128-33. 2006

Collaborators

Detail Information

Publications34

  1. pmc Identification and functional study of a new missense mutation in the motor head domain of myosin VIIA in a family with autosomal dominant hearing impairment (DFNA11)
    Qing Sang
    State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, People s Republic of China
    PLoS ONE 8:e55178. 2013
    ..In the actin-activated ATPase activity assay, the rate of NADH oxidation was higher in the wild-type myosin VIIA, indicating that the ATPase activity in the p.R668H mutant myosin VIIA was significantly destroyed...
  2. pmc Differentiation of neurons from neural precursors generated in floating spheres from embryonic stem cells
    Huawei Li
    Department of Otolarygology, EENT Hospital of Shanghai Medical College of Fudan University, Institute of Biomedical Sciences, Fudan University Shanghai, 200031, PR China
    BMC Neurosci 10:122. 2009
    ..Obtaining reproducible percentages of neural cells is difficult and the methods are time consuming...
  3. pmc BMP4 signaling is involved in the generation of inner ear sensory epithelia
    Huawei Li
    Department of Otolaryngology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
    BMC Dev Biol 5:16. 2005
    ..Homozygous BMP4-/- animals die before the inner ear's sensory organs develop, which precludes determining the role of BMP4 in these organs with simple gene knockout experiments...
  4. ncbi request reprint Characterization of proliferating cells from newborn mouse cochleae
    Zhengmin Wang
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, PR China
    Neuroreport 17:767-71. 2006
    ..The sphere-forming cells being capable of differentiating into hair cell-like cells implies the possibility of using these sphere-forming cells for reconstructing the damaged cochlear hair cells...
  5. ncbi request reprint Conditioned medium from neonatal rat olfactory ensheathing cells promotes the survival and proliferation of spiral ganglion cells
    Hongmeng Yu
    Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai
    Acta Otolaryngol 130:351-7. 2010
    ..Neonatal olfactory ensheathing cells conditioned medium (OEC-CM) can promote growth and proliferation of neonatal spiral ganglion cells (SGCs), as well as protecting them from apoptosis...
  6. ncbi request reprint Islet-1 expression in the developing chicken inner ear
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School, and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    J Comp Neurol 477:1-10. 2004
    ..Our data is consistent with a role for islet-1 in differentiating inner ear neurons and sensory epithelia cells, perhaps in the specification of cellular subtypes in conjunction with other LIM/homeodomain proteins...
  7. pmc Engraftment and differentiation of embryonic stem cell-derived neural progenitor cells in the cochlear nerve trunk: growth of processes into the organ of Corti
    C Eduardo Corrales
    Department of Otology and Laryngology, Harvard Medical School, Boston, MA 02115, USA
    J Neurobiol 66:1489-500. 2006
    ..The regeneration of these neurons shows that neurons differentiated from stem cells have the capacity to grow to a specific target in an animal model of neuronal degeneration...
  8. pmc Cotransfection of Pax2 and Math1 promote in situ cochlear hair cell regeneration after neomycin insult
    Yan Chen
    1 Otology Skull base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University Shanghai, 200031, P R China 2 Central laboratory, Eye and ENT Hospital of Shanghai Medical School, Fudan University Shanghai, 200031, P R China 3
    Sci Rep 3:2996. 2013
    ..The results also suggest that inducing proliferating cells rather than quiescent cells to differentiate into hair cells by forced expression of Math1 is feasible for mammalian hair cell regeneration...
  9. doi request reprint A novel mutation in the MITF may be digenic with GJB2 mutations in a large Chinese family of Waardenburg syndrome type II
    Xukun Yan
    Department of Otolaryngology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China
    J Genet Genomics 38:585-91. 2011
    ..Thus, a novel compound heterozygous mutation, c.[742_743delAAinsT;746_747delCA] in MITF exon 8 was the key genetic reason for WS2 in this family, and a digenic effect of MITF and GJB2 genes may contribute to deafness of the proband...
  10. ncbi request reprint Peripherin as a marker for degeneration of spiral ganglion neurons after aminoglycoside ototoxicity
    Yucheng Wang
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai
    Acta Otolaryngol 126:1128-33. 2006
    ..Our data show that temporary appearance of atypical type 1 neurons, like type 3 neurons, might be another degenerating form of spiral ganglion neurons (SGNs); peripherin might be a marker of degenerating neurons...
  11. doi request reprint GJB2 allele variants and the associated audiologic features identified in Chinese patients with less severe idiopathic hearing loss
    Jianning Zhang
    Department of Otolaryngology, Eye Ear Nose and Throat Hospital of Fudan University, 83 Fen Yang Road, Shanghai, China
    Genet Test Mol Biomarkers 15:313-8. 2011
    ....
  12. doi request reprint Early postnatal virus inoculation into the scala media achieved extensive expression of exogenous green fluorescent protein in the inner ear and preserved auditory brainstem response thresholds
    Yunfeng Wang
    Department of Otolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, PR China
    J Gene Med 15:123-33. 2013
    ..The present study aimed to define a suitable strategy for preserving hearing after viral inoculation directly into the scala media performed at various postnatal developmental stages...
  13. pmc Generation of hair cells by stepwise differentiation of embryonic stem cells
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 100:13495-500. 2003
    ....
  14. ncbi request reprint Correlation of Pax-2 expression with cell proliferation in the developing chicken inner ear
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, 02114, USA
    J Neurobiol 60:61-70. 2004
    ..Furthermore, the likelihood that Pax-2-expressing cells in the otocyst die by apoptosis is much lower when compared with cells residing in Pax-2-negative regions...
  15. pmc Diverse expression patterns of LIM-homeodomain transcription factors (LIM-HDs) in mammalian inner ear development
    Mingqian Huang
    Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary and Department of Otology and Laryngology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Dev Dyn 237:3305-12. 2008
    ..With the exception of Lmx1a and Lhx3, all LIM-HDs are expressed in ganglion neurons. Expression of multiple LIM-HDs within a cell type suggests their redundant function...
  16. pmc A six-generation Chinese family in haplogroup B4C1C exhibits high penetrance of 1555A > G-induced hearing Loss
    Yan Bai
    Department of Otolaryngology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China
    BMC Med Genet 11:129. 2010
    ..Environmental factors, nuclear genes, mitochondrial haplotypes/variants and a possible threshold effect have been reported to may be involved in its manifestation...
  17. doi request reprint Mouse auditory organ development required bone morphogenetic protein signaling
    Shaofeng Liu
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, PR China
    Neuroreport 22:396-401. 2011
    ..Cell proliferation and cell death were not changed significantly by BMP signaling. Collectively, our results indicated that BMP signaling is essential to cochlear sensory formation and hair cell differentiation...
  18. ncbi request reprint Generation of inner ear cell types from embryonic stem cells
    Marcelo N Rivolta
    Department of Otolaryngology and Program in Neuroscience, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, USA
    Methods Mol Biol 330:71-92. 2006
    ..Inner ear progenitor cells, generated from ES cells, may be used for the development of cell replacement therapy for the diseased inner ear, for high-throughput drug screening, and for the study of inner ear development...
  19. doi request reprint PORP vs. TORP: a meta-analysis
    Huiqian Yu
    Department of Otorhinolaryngology, Shanghai Eye and ENT Hospital, Shanghai Medical College, Fudan University, 200031, Shanghai, China
    Eur Arch Otorhinolaryngol 270:3005-17. 2013
    ..In addition, the stability of PORP was significantly superior to TORP in long-term follow-ups, but no significant effect was detected in the general study...
  20. ncbi request reprint Survival-enhancing of spiral ganglion cells under influence of olfactory ensheathing cells by direct cellular contact
    Quan Liu
    Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Shanghai Medical College of Fudan University, 83 Fen Yang Road, Shanghai 200031, PR China
    Neurosci Lett 478:37-41. 2010
    ..In conclusion, our results suggest that enhancement in co-cultures is in part due to direct cellular contact. Transplantation of OECs may be a cell-based therapy for the application of neurotrophic factors to the inner ear...
  21. ncbi request reprint Surgery for endolymphatic sac tumor: whether and when to keep hearing?
    Yusu Ni
    Otology Skull Base Surgery Department, Central Laboratory, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China
    Acta Otolaryngol 128:976-83. 2008
    ..Endolymphatic sac tumors (ELSTs) are locally invasive, osteolytic, and hypervascular tumors. If the labyrinth has not been invaded, the hearing should be preserved in operation...
  22. pmc Disrupting the interaction between retinoblastoma protein and Raf-1 leads to defects in progenitor cell proliferation and survival during early inner ear development
    Wenyan Li
    Department of Otorhinolaryngology, Affiliated Eye and ENT Hospital of Fudan University, Shanghai, China Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States of America
    PLoS ONE 8:e83726. 2013
    ..Our study provides novel routes to modulate pRb function for hair cell regeneration. ..
  23. ncbi request reprint Stem cells as therapy for hearing loss
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
    Trends Mol Med 10:309-15. 2004
    ....
  24. ncbi request reprint Correlation of expression of the actin filament-bundling protein espin with stereociliary bundle formation in the developing inner ear
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School, and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    J Comp Neurol 468:125-34. 2004
    ....
  25. pmc A low-cost exon capture method suitable for large-scale screening of genetic deafness by the massively-parallel sequencing approach
    Wenxue Tang
    Department of Otolaryngology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Genet Test Mol Biomarkers 16:536-42. 2012
    ..The high coverage depth and cost benefits of the cDNA-probe-based exon capture approach may also facilitate widespread applications in clinical practices beyond screening mutations in deafness genes...
  26. ncbi request reprint Role of histone deacetylase activity in the developing lateral line neuromast of zebrafish larvae
    Yingzi He
    1 Department of Otology Skull Base Surgery, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China 2 Institutes of Biomedical Sciences, Fudan University, Shanghai, China
    Exp Mol Med 46:e94. 2014
    ....
  27. ncbi request reprint Pluripotent stem cells from the adult mouse inner ear
    Huawei Li
    Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    Nat Med 9:1293-9. 2003
    ..Our observation that these stem cells are capable of differentiating into hair cell-like cells implies a possible use of such cells for the replacement of lost inner-ear sensory cells...
  28. ncbi request reprint [Characteristic of the vertical and pyramidal segment of facial nerve canal]
    Yusu Ni
    Surgery of Ear Skull base, Affiliated Eye Ear Nose Throat Hospital, Fudan University, Shanghai, 200031, China
    Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24:1009-11, 1015. 2010
    ..To study the vertical and pyramidal segment of facial nerve canal based on three-dimensional reconstruction of temporal bone CT, and provide a quantitative data for the middle ear and mastoid surgery...
  29. doi request reprint Disrupting Rb-Raf-1 interaction inhibits hair cell regeneration in zebrafish lateral line neuromasts
    Qin Lin
    Department of Otolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, PR China
    Neuroreport 24:190-5. 2013
    ..Our results indicate that Rb-Raf-1 interaction plays an important role in spontaneous HC regeneration in zebrafish...
  30. ncbi request reprint [Clinical experience in facial nerve tumors: a review of 27 cases]
    Fan Zhang
    Department of Otolaryngology, Eye Ear Nose Throat Hospital of Fudan University, Shanghai, 200031, China
    Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24:4-7. 2010
    ..To analyze the clinical manifestations and the diagnosis of the facial nerve tumor according to the clinical information, and evaluate the different surgical approaches depending on tumor location...
  31. doi request reprint Quantitative morphology of facial nerve based on three-dimensional reconstruction of temporal bone
    Yusu Ni
    Otology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China
    Otolaryngol Head Neck Surg 138:23-9. 2008
    ..To investigate an effective method of quantitative analysis of the facial nerve and its adjacent structures on the basis of three-dimensional (3D) CT image reconstruction of the temporal bone...
  32. pmc Sonic hedgehog initiates cochlear hair cell regeneration through downregulation of retinoblastoma protein
    Na Lu
    Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031, China
    Biochem Biophys Res Commun 430:700-5. 2013
    ..This study provides an additional route to modulate pRb function with important implications in mammalian hair cell regeneration...
  33. pmc Targeted connexin26 ablation arrests postnatal development of the organ of Corti
    Yunfeng Wang
    Department of Otolaryngology, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322 3030, USA
    Biochem Biophys Res Commun 385:33-7. 2009
    ..These results demonstrated that Cx26 plays essential roles in postnatal maturation and homoeostasis of the organ of Corti before the onset of hearing...
  34. ncbi request reprint Endoscopic surgery with powered instrumentation for isolated sphenoid sinus disease
    Hongmeng Yu
    Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, China
    ORL J Otorhinolaryngol Relat Spec 68:129-34. 2006
    ..In this study, we investigate the diagnosis and pathology of ISSD and compare endoscopic treatment with powered instrumentation with conventional surgical instruments for ISSD...