Jian He Xu

Summary

Affiliation: East China University of Science and Technology
Country: China

Publications

  1. Ni Y, Yu H, Lin G, Xu J. An ene reductase from Clavispora lusitaniae for asymmetric reduction of activated alkenes. Enzyme Microb Technol. 2014;56:40-5 pubmed publisher
    ..All these positive features demonstrate a high synthetic potential of ClER in the asymmetric reduction of activated alkenes. ..
  2. Chen Q, Luan Z, Yu H, Cheng X, Xu J. Rational design of a carboxylic esterase RhEst1 based on computational analysis of substrate binding. J Mol Graph Model. 2015;62:319-24 pubmed publisher
    ..This work provides molecular-level insights into the substrate binding mechanism of carboxylic esterase RhEst1, facilitating future experimental efforts toward developing more efficient RhEst1 variants for industrial applications. ..
  3. Luo X, Zhao J, Li C, Bai Y, Reetz M, Yu H, et al. Combinatorial evolution of phosphotriesterase toward a robust malathion degrader by hierarchical iteration mutagenesis. Biotechnol Bioeng. 2016;113:2350-7 pubmed publisher
    ..6°C. Thus, PoOPHM9 appears to be an efficient and robust candidate for malathion detoxification. Biotechnol. Bioeng. 2016;113: 2350-2357. © 2016 Wiley Periodicals, Inc. ..
  4. Liu X, Chen F, Li C, Luo X, Chen Q, Bai Y, et al. Improved efficiency of a novel methyl parathion hydrolase using consensus approach. Enzyme Microb Technol. 2016;93-94:11-17 pubmed publisher
    ..This study generated an efficient MP-degrading enzyme, and provides useful information for enhancing the catalytic efficiency of MPHs via conservative residue substitution based on the consensus approach. ..
  5. Jiao X, Pan J, Kong X, Xu J. Protein engineering of aldolase LbDERA for enhanced activity toward real substrates with a high-throughput screening method coupled with an aldehyde dehydrogenase. Biochem Biophys Res Commun. 2017;482:159-163 pubmed publisher
    ..The crystal structure of LbDERAT29L/163Y obtained by X-ray diffraction with 1.77 Å resolution revealed the structural changes responsible for the significant activity improvement. ..
  6. Chen K, Zheng M, Pan J, Li C, Xu J. Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester. Appl Biochem Biotechnol. 2017;183:543-554 pubmed publisher
    ..The significantly improved volumeric production and specific activity of S. marcescens lipase make it very attractive as a new-generation biocatalyst for more efficient and economical manufacturing of (-)-MPGM. ..
  7. Yu H, Li T, Chen F, Luo X, Li A, Yang C, et al. Bioamination of Alkane with Ammonium by an Artificially Designed Multienzyme Cascade. Metab Eng. 2018;: pubmed publisher
    ..This artificially designed bioamination paves an attractive approach for enzymatic synthesis of amines from accessible and cheap alkanes. ..

Detail Information

Publications7

  1. Ni Y, Yu H, Lin G, Xu J. An ene reductase from Clavispora lusitaniae for asymmetric reduction of activated alkenes. Enzyme Microb Technol. 2014;56:40-5 pubmed publisher
    ..All these positive features demonstrate a high synthetic potential of ClER in the asymmetric reduction of activated alkenes. ..
  2. Chen Q, Luan Z, Yu H, Cheng X, Xu J. Rational design of a carboxylic esterase RhEst1 based on computational analysis of substrate binding. J Mol Graph Model. 2015;62:319-24 pubmed publisher
    ..This work provides molecular-level insights into the substrate binding mechanism of carboxylic esterase RhEst1, facilitating future experimental efforts toward developing more efficient RhEst1 variants for industrial applications. ..
  3. Luo X, Zhao J, Li C, Bai Y, Reetz M, Yu H, et al. Combinatorial evolution of phosphotriesterase toward a robust malathion degrader by hierarchical iteration mutagenesis. Biotechnol Bioeng. 2016;113:2350-7 pubmed publisher
    ..6°C. Thus, PoOPHM9 appears to be an efficient and robust candidate for malathion detoxification. Biotechnol. Bioeng. 2016;113: 2350-2357. © 2016 Wiley Periodicals, Inc. ..
  4. Liu X, Chen F, Li C, Luo X, Chen Q, Bai Y, et al. Improved efficiency of a novel methyl parathion hydrolase using consensus approach. Enzyme Microb Technol. 2016;93-94:11-17 pubmed publisher
    ..This study generated an efficient MP-degrading enzyme, and provides useful information for enhancing the catalytic efficiency of MPHs via conservative residue substitution based on the consensus approach. ..
  5. Jiao X, Pan J, Kong X, Xu J. Protein engineering of aldolase LbDERA for enhanced activity toward real substrates with a high-throughput screening method coupled with an aldehyde dehydrogenase. Biochem Biophys Res Commun. 2017;482:159-163 pubmed publisher
    ..The crystal structure of LbDERAT29L/163Y obtained by X-ray diffraction with 1.77 Å resolution revealed the structural changes responsible for the significant activity improvement. ..
  6. Chen K, Zheng M, Pan J, Li C, Xu J. Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester. Appl Biochem Biotechnol. 2017;183:543-554 pubmed publisher
    ..The significantly improved volumeric production and specific activity of S. marcescens lipase make it very attractive as a new-generation biocatalyst for more efficient and economical manufacturing of (-)-MPGM. ..
  7. Yu H, Li T, Chen F, Luo X, Li A, Yang C, et al. Bioamination of Alkane with Ammonium by an Artificially Designed Multienzyme Cascade. Metab Eng. 2018;: pubmed publisher
    ..This artificially designed bioamination paves an attractive approach for enzymatic synthesis of amines from accessible and cheap alkanes. ..