Ju Chu

Summary

Affiliation: East China University of Science and Technology
Country: China

Publications

  1. doi request reprint Progress in the research of S-adenosyl-L-methionine production
    Ju Chu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P O Box 329, 200237, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 97:41-9. 2013
  2. doi request reprint A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production
    Yiming Yang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
    J Biotechnol 161:250-6. 2012
  3. doi request reprint Regulation of branched-chain amino acid catabolism: glucose limitation enhances the component of isovalerylspiramycin for the bitespiramycin production
    Yong Hong Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioprocess Biosyst Eng 33:257-65. 2010
  4. doi request reprint DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris
    Hui Hu
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai, PR China
    J Biotechnol 141:97-103. 2009
  5. ncbi request reprint Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in HEK-293 cells using polyethyleneimine-DNA complexes in combination with hypothermic treatment
    Lei Feng
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Biotechnol Appl Biochem 50:121-32. 2008
  6. doi request reprint Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale
    Xiang Zou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Bioresour Technol 100:1406-12. 2009
  7. doi request reprint Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding
    Kun Tai Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China College of Biological Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
    Bioresour Technol 99:8516-20. 2008
  8. doi request reprint Influence of high solid concentration on enzymatic hydrolysis and fermentation of steam-exploded corn stover biomass
    Yifeng Lu
    East China University of Science and Technology, Shanghai, China
    Appl Biochem Biotechnol 160:360-9. 2010
  9. doi request reprint Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea
    Xiang Zou
    East China University of Science and Technology, Shanghai, People s Republic of China
    J Ind Microbiol Biotechnol 35:1637-42. 2008
  10. doi request reprint An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B(12) by Pseudomonas denitrificans
    Kun Tai Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 31:605-10. 2008

Collaborators

Detail Information

Publications69

  1. doi request reprint Progress in the research of S-adenosyl-L-methionine production
    Ju Chu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P O Box 329, 200237, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 97:41-9. 2013
    ..Precise control of gene expression and quantitative assessment of physiological parameters in engineered P. pastoris were highlighted. Finally, a discussion of the prospect of SAM production was presented...
  2. doi request reprint A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production
    Yiming Yang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
    J Biotechnol 161:250-6. 2012
    ..Power consumption was dramatically decreased by 25% by using novel impeller configuration...
  3. doi request reprint Regulation of branched-chain amino acid catabolism: glucose limitation enhances the component of isovalerylspiramycin for the bitespiramycin production
    Yong Hong Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioprocess Biosyst Eng 33:257-65. 2010
    ..These suggested that glucose concentration could be used as a key parameter to regulate BCKDH activity and isovaleryispiramycin biosynthesis in the bitespiramycin production...
  4. doi request reprint DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris
    Hui Hu
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai, PR China
    J Biotechnol 141:97-103. 2009
    ..Finally, a 6.14gl(-1) of SAM production was reached in a 500l bioreactor with the best recombinant strain GS115/DS56, which showed a favorable foreground in industrial scale production of SAM...
  5. ncbi request reprint Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in HEK-293 cells using polyethyleneimine-DNA complexes in combination with hypothermic treatment
    Lei Feng
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Biotechnol Appl Biochem 50:121-32. 2008
    ..48+/-0.59)x10(11) physical particles or 1.86+/-0.96x10(10) infectious particles were achieved in 250 ml shake flasks with 60 ml working volume, indicating a promising application for scale-up...
  6. doi request reprint Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale
    Xiang Zou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Bioresour Technol 100:1406-12. 2009
    ..Erythromycin production on industrial scale was similar to that at laboratory scale...
  7. doi request reprint Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding
    Kun Tai Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China College of Biological Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
    Bioresour Technol 99:8516-20. 2008
    ....
  8. doi request reprint Influence of high solid concentration on enzymatic hydrolysis and fermentation of steam-exploded corn stover biomass
    Yifeng Lu
    East China University of Science and Technology, Shanghai, China
    Appl Biochem Biotechnol 160:360-9. 2010
    ..9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production...
  9. doi request reprint Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea
    Xiang Zou
    East China University of Science and Technology, Shanghai, People s Republic of China
    J Ind Microbiol Biotechnol 35:1637-42. 2008
    ..7% higher than the control in the same cultivation conditions. It was the first report to integrate physiological parameter OUR and statistical methods to optimize erythromycin production...
  10. doi request reprint An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B(12) by Pseudomonas denitrificans
    Kun Tai Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 31:605-10. 2008
    ..When the new pH control strategy was applied, pH was stably kept in the range of 7.15-7.30 during fermentation. Thus, 214.3 microg/mL of vitamin B(12) was achieved...
  11. pmc Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus
    Lan Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China
    BMC Biotechnol 12:52. 2012
    ..To our knowledge, none of the ABC transporters in S. rimosus have yet been characterized. In this study, we aimed to characterize the multidrug exporter function of OtrC and evaluate its relevancy to OTC production...
  12. doi request reprint Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase
    Xiang Zou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Bioresour Technol 100:3358-65. 2009
    ..The maximal Er-A production reached 7938 U/ml at 203 h, which was enhanced by 22.1% compared with the control (6501 U/ml at 192 h). The ratio of Er-A to Er-C was 24.05:1, which was higher than that of the control (4.77:1)...
  13. doi request reprint Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes
    Lan Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
    Enzyme Microb Technol 50:318-24. 2012
    ..2% and 32.9% in M4018 and SR16 mutants respectively). These data indicate that the minimal PKS plays an important role in carbon flux redirection from cell growth pathways to OTC biosynthesis pathways...
  14. doi request reprint Improved vitamin B(12) production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process
    Ze Jian Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Bioresour Technol 101:2845-52. 2010
    ..4% lower than that of the control. An efficient and economical fermentation process based on OUR criterion was established for industrial vitamin B(12) fermentation by P. denitrificans...
  15. doi request reprint Effects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by P(GAP)-driven Pichia pastoris overexpressing methionine adenosyltransferase
    Xiao qing Hu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P R China
    J Biotechnol 137:44-9. 2008
    ..Strategy E also led to the highest values of ATP and biomass besides MAT. Consequently, the highest SAM yield and volumetric level were obtained at 0.058gg(-1) and 9.26gl(-1), respectively...
  16. doi request reprint Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-alpha mutant by Pichia pastoris
    Dan Wu
    State Key Laboratory of Bioreactor Engineering, National Engineering Center of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Microbiol Biotechnol 85:1759-67. 2010
    ..These results indicate that the incomplete formation of disulfide bond in cIFN secreted to fermentation broth triggers severe degradation and aggregation of cIFN, which result in sharp decrease of bioactivity of cIFN in bioreactor...
  17. doi request reprint Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil
    Jianhua Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 33:1119-29. 2010
    ..Therefore, the improvement of CPC production yield through optimization of the NADPH, ATP generation, and consumption was put forward...
  18. doi request reprint Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol
    Zhi Qiang Xiong
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, PR China
    Bioresour Technol 100:1011-4. 2009
    ..Hence, ethanol extraction which does not disrupt yeast cells could be an inexpensive, simple and efficient alternative to conventional extraction techniques in the GSH industry...
  19. doi request reprint High-throughput system for screening of Cephalosporin C high-yield strain by 48-deep-well microtiter plates
    Jun Tan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People s Republic of China
    Appl Biochem Biotechnol 169:1683-95. 2013
    ..The CPC production of strain W-6 was further validated in 50 l bioreactor, and the CPC production reached 32.0 g/l, twofold higher than that of the wild strain...
  20. pmc Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris
    Jin Zhou
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai 200237, P R China
    Biotechnol Lett 30:651-6. 2008
    ..Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo...
  21. doi request reprint Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway
    Zhenyu Tang
    State Key Laboratory of Bioreactor Engineering, P O Box 329, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China
    Enzyme Microb Technol 49:17-24. 2011
    ....
  22. doi request reprint Isolation of soluble proteins from an industrial strain Streptomyces avermitilis in complex culture medium for two-dimensional gel electrophoresis
    Peng Yin
    State Key Laboratory of Bioreactor Engineering, National Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai 200237, PR China
    J Microbiol Methods 73:105-10. 2008
    ..With the optimized protein extraction protocol, the protein expression patterns of S. avermitilis during the onset of avermectin production in complex medium were analyzed...
  23. doi request reprint Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14
    Jin Zhou
    State Key Laboratory of Bioreactor Engineering, National Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai 200237, PR China
    Bioresour Technol 99:6826-33. 2008
    ..It deserved noting that the beta-glucosidase from the T 100-14 displayed an extremely high activity on p-nitrophenyl-beta-D-glycopyranoside (pNPG), which suggested it was a good candidate for the conversion of cellobiose to glucose...
  24. doi request reprint Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli
    Jie Zhao
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, People s Republic of China
    J Ind Microbiol Biotechnol 35:257-62. 2008
    ..Under these proposed optimized medium, the model predicted a PyOD activity of 610 U/L and via experimental rechecking the model, an activity of 670 U/L was attained...
  25. ncbi request reprint [Quantitative studies of the production phase in rHSA fermentation]
    Ming Zhi Huang
    State Key Laboratory of Bioreactor Engineering, National Center of Biotechnology, Bioengineering College, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 19:81-6. 2003
    ..The model could preferably described the relations between different macroscopic reaction rates of the process and keys for the high-efficiency expression of HAS were deduced...
  26. doi request reprint Inhibition of degradation and aggregation of recombinant human consensus interferon-alpha mutant expressed in Pichia pastoris with complex medium in bioreactor
    Dan Wu
    State Key Laboratory of Bioreactor Engineering, National Engineering Center of Biotechnology, East China University of Science and Technology, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 80:1063-71. 2008
    ..35 x 10(8) +/- 0.56 x 10(8) IU mg(-1) was obtained. These results should be applicable to other low-stability recombinant proteins expressed in P. pastoris...
  27. doi request reprint An assessment of seed quality on erythromycin production by recombinant Saccharopolyspora erythraea strain
    Xiang Zou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Bioresour Technol 102:3360-5. 2011
    ..63% with respect to the control. It was the first report to integrate cell growth characteristics and physiological response method to assess the seed quality for erythromycin production...
  28. doi request reprint Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes
    Taicheng Zhu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P O Box 329, 130 Meilong Rd, Shanghai 200237, People s Republic of China
    Appl Microbiol Biotechnol 89:1127-35. 2011
    ..These data suggested that high copy P. pastoris strain might be suffering from protein folding-related oxidative stress and insufficient supply of carbon and energy sources...
  29. doi request reprint Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium
    Zhi Qiang Xiong
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, P R China
    J Biosci Bioeng 105:409-13. 2008
    ..Therefore, it is concluded that a capacitance probe is a practical tool for real-time viable biomass monitoring in high-cell-density fed-batch cultivation in a complex medium...
  30. pmc Genetic modulation of the overexpression of tailoring genes eryK and eryG leading to the improvement of erythromycin A purity and production in Saccharopolyspora erythraea fermentation
    Yun Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Appl Environ Microbiol 74:1820-8. 2008
    ....
  31. ncbi request reprint Intracellular expression of Vitreoscilla hemoglobin improves S-adenosylmethionine production in a recombinant Pichia pastoris
    Huaxin Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 74:1205-12. 2007
    ..We suggested that under our experimental scheme, the presence of VHb might improve ATP synthesis rate and thus improve cell growth and SAM production in the recombinant P. pastoris...
  32. pmc GAP promoter library for fine-tuning of gene expression in Pichia pastoris
    Xiulin Qin
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Appl Environ Microbiol 77:3600-8. 2011
    ..Thus, it is a useful toolbox for both basic and applied research in P. pastoris...
  33. doi request reprint Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments
    Jin Zhou
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology Shanghai, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China
    Bioresour Technol 100:819-25. 2009
    ..The enhanced cellulolytic activity displayed by the constructed cellulase mixture could be used as an effective tool for producing bioethanol efficiently from cellulose...
  34. doi request reprint Optimization of L: -methionine feeding strategy for improving S-adenosyl-L: -methionine production by methionine adenosyltransferase overexpressed Pichia pastoris
    Hui Hu
    National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai, People s Republic of China
    Appl Microbiol Biotechnol 83:1105-14. 2009
    ..The MAT activity also decreased as the L: -Met feeding rate rose. The reduced ATP synthesis and MAT activity were probably the reason for the low SAM accumulation when the L: -Met feeding rate reached 0.5 g l(-1) h(-1)...
  35. doi request reprint Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens
    Ben Rao
    State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People s Republic of China
    Appl Microbiol Biotechnol 93:2147-59. 2012
    ..The obtained results and further investigations should lead to a more suitable fermentation strategy and strain improvement which would be applicable to the industrial production of 2,3-butanediol...
  36. ncbi request reprint The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation
    Yuyou Hao
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P O Box 329, Shanghai People s Republic of China
    Appl Microbiol Biotechnol 74:578-84. 2007
    ..The maximum specific activity with Tween-20 at 20 degrees C was 19.9-fold higher than that without Tween-20 at 30 degrees C...
  37. ncbi request reprint [Relationship between key enzyme activities of inosine-producing pathway and inosine accumulation]
    Yongbo Song
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Wei Sheng Wu Xue Bao 43:361-5. 2003
    ..Enzymology study is believed to be an effective way to make screening of high-yield strains more efficient...
  38. ncbi request reprint [Carbon and energetic metabolism of Synechococcus sp. PCC7942 under photoautotrophic conditions]
    Riming Yan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 25:1352-9. 2009
    ..80 g/mol and 2.97 g/mol respectively under the appointed incident intensity. But the cell yield on absorbed light energy their corresponding energy conversion efficiency were descended with the increasing of incident intensity...
  39. ncbi request reprint Improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase
    Yong Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Appl Microbiol Biotechnol 75:837-42. 2007
    ..The sporulation process was inhibited, while the SAM-s gene was expressed. The addition of the exogenous SAM also inhibited sporulation and promoted an increase in erythromycin titers...
  40. doi request reprint A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains
    Taicheng Zhu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd, P O Box 329, Shanghai, 200237, People s Republic of China
    Biotechnol Lett 31:679-84. 2009
    ..In 5-l fermentor culture, the ACNs of PIP gene in A2, A3, C3 were also decreased to 10, 15, 21 copies, respectively, after 72 h of methanol induction...
  41. ncbi request reprint [The influence of controlling redox potential on ethanol production by Saccharomyces cerevisiae]
    Yang Yu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 23:878-84. 2007
    ..So it give us a apocalypse that we can use redox electrode to control the ethanol fermentation exactly on bioreactor scale...
  42. ncbi request reprint Fermentation process optimization of recombinant Saccharomyces cerevisiae for the production of human interferon-alpha2a
    Ju Chu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
    Appl Biochem Biotechnol 111:129-38. 2003
    ..3 x 10(7) IU/mL of biologic activity was achieved. In the expression phase, pH had an important impact on expression level, which should be controlled at 5.5...
  43. doi request reprint Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m3 fermenter
    Xiang Zou
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 35:789-800. 2012
    ..These results were helpful for understanding the relationship between hydrodynamic environment and physiological response of cells in bioreactor during the scale-up of fermentation process...
  44. doi request reprint Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5
    Xu Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China
    Bioresour Technol 102:1142-8. 2011
    ..082×10(5) to 0.944×10(5) Pa, which indicated that reduction of H2 partial pressure by lowering the operating pressure substantially improved H2 production in an anaerobic, photo-fermentation process...
  45. ncbi request reprint [Regulatory effects of ammonium ions on the biosynthesis of meilingmycin]
    Ping Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Wei Sheng Wu Xue Bao 45:405-9. 2005
    ....
  46. ncbi request reprint [Quantitation & optimization of guanosine fermentation process: prevention of NH4+ accumulation increases guanosine production by 70%]
    Ming Zhi Huang
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology, and Bioengineering College, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 19:200-5. 2003
    ..Accordingly, measures were taken to prevent the accumulation of NH4+. The interference indeed stopped the metabolism shift and boosted the guanosine production at 30 g/L, 70% higher than the level reported in literature...
  47. doi request reprint Industrial bioprocess control and optimization in the context of systems biotechnology
    Yong Wang
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China
    Biotechnol Adv 27:989-95. 2009
    ..The holistic viewpoints and ideas applied in industrial bioprocesses and their future prospects are discussed by illustrating some successful cases...
  48. ncbi request reprint Optimization of transfection mediated by calcium phosphate for plasmid rAAV-LacZ (recombinant adeno-associated virus-beta-galactosidase reporter gene) production in suspension-cultured HEK-293 (human embryonic kidney 293) cells
    Lei Feng
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Biotechnol Appl Biochem 46:127-35. 2007
    ..g. (vector genomes)/60 ml of medium respectively, is promising for the technique's application in the large-scale production of rAAV...
  49. doi request reprint Leucine improves the component of isovalerylspiramycins for the production of bitespiramycin
    Zhen lin Li
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 32:641-7. 2009
    ....
  50. doi request reprint Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis
    Yong Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P O Box 329, 200237, Shanghai, People s Republic of China
    Bioprocess Biosyst Eng 36:1445-53. 2013
    ....
  51. ncbi request reprint Expression and aggregation of recombinant human consensus interferon-alpha mutant by Pichia pastoris
    Yuyou Hao
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Biotechnol Lett 28:905-9. 2006
    ..When the fermentation supernatant was disaggregated with 6 M guanidine hydrochloride, the antiviral activity of cIFN achieved 2.2 x 10(8) IU ml(-1)...
  52. ncbi request reprint Ethanol evolution rate: a new parameter to determine the feeding rate for the production of avermectins by Streptomyces avermitilis
    Jie Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Biotechnol Lett 26:109-13. 2004
    ..Together with other criteria, the EER helped to control utilization of substrate, so it has been successfully used to control glucose feeding in an industrial process...
  53. doi request reprint Production and distribution of beta-glucosidase in a mutant strain Trichoderma viride T 100-14
    Yong Hong Wang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    N Biotechnol 26:150-6. 2009
    ..Additionally, the secretion ratio (maximal secreted beta-glucosidase activity/maximal total activity) with pH control or 2-deoxy-d-glucose addition was elevated profoundly near to a level as the cellulase in fungi...
  54. ncbi request reprint Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains
    Jiangchao Qian
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Biotechnol Lett 28:937-41. 2006
    ..These mutations may explain the purine accumulation in purine-producing strains and be helpful to the rational design of high-yield recombinant strains...
  55. ncbi request reprint [Over-expression in Escherichia coli and characterization of apolipoprotein AI]
    Man Sheng Ding
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 21:198-203. 2005
    ....
  56. pmc Time-resolved transcriptome analysis of Bacillus subtilis responding to valine, glutamate, and glutamine
    Bang Ce Ye
    Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
    PLoS ONE 4:e7073. 2009
    ..The results revealed the common and distinct modes of action of these three amino acids, and should help to elucidate the specific signaling mechanism of each amino acid as an effector...
  57. doi request reprint Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30
    Liaoyuan Zhang
    State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China
    Bioresour Technol 101:1961-7. 2010
    ..Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC+BD) productivity of 3.49 g/lh and the yield of 94.67% was obtained...
  58. ncbi request reprint [Carbon metabolism and energetic utilization of Synechococcus sp. PCC7942 under mixotrophic condition]
    Riming Yan
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 26:1239-48. 2010
    ..The energy conversion efficiencies based on ATP synthesis in photoautotrophic culture, mixotrophic cultures with glucose and with acetate were evaluated to be 6.81%, 7.43% and 8.77%, respectively...
  59. ncbi request reprint [Influence of Mn2+ on the biotechmycin fermentation]
    Yuan Kang
    State Key Laboratory of Bioreactor Engineering, National Engineering Centre for Biotechnology Shanghai, East China University of Science and Technology, Shanghai 200237, China
    Wei Sheng Wu Xue Bao 45:81-5. 2005
    ..Therefore, it can be concluded that Mn2+ has profound influence on the biosynthesis of biotechmycin: it enriches the biotechmycin precursor pool such as propionic acid and thus improves the yield of biotechmycin...
  60. doi request reprint Cloning and characterization of Kluyveromyces marxianus Hog1 gene
    Jiangchao Qian
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People s Republic of China
    Biotechnol Lett 33:571-5. 2011
    ..However, neither strain grew at 47°C. Kmhog1 may thus be required for adaptation to acetic acid, osmotic, and oxidative stress but is not involved in thermotolerance...
  61. ncbi request reprint [Analysis on metabolic flux shift during guanosine fermentation]
    Xian Peng Cai
    State Key Laboratory of Bioreactor Engineering, ECUST, National Engineering Research Center for Biotechnology, Shanghai 200237, China
    Sheng Wu Gong Cheng Xue Bao 18:622-5. 2002
    ..The subsequent optimization based on controlling flux distribution between EMP and HMP did improve the yield by 35% when the metabolic flux shift was prevented...
  62. doi request reprint Bioreactors and bioseparation
    Siliang Zhang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Adv Biochem Eng Biotechnol 122:105-50. 2010
    ..The preparation of bioseparation materials, as well as part of bioseparation process research development in the past 5 years, are introduced here...
  63. ncbi request reprint A multi-scale study of industrial fermentation processes and their optimization
    Siliang Zhang
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China
    Adv Biochem Eng Biotechnol 87:97-150. 2004
    ..Substantial improvement of industrial fermentation productivity was achieved...
  64. ncbi request reprint [Phase transfer catalyzed bioconversion of penicillin G to 6-APA by immobilized penicillin acylase in recyclable aqueous two-phase systems with light/pH sensitive copolymers]
    Ke ming Jin
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Nan Fang Yi Ke Da Xue Xue Bao 28:360-2. 2008
    ..1). The recovery of the two copolymers was between 95% and 99%...
  65. doi request reprint Improvement of innate immune responses and defense activity in mitten crab (Eriocheir sinensis) by oral administration of beta-glucan
    Jin Zhou
    State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology Shanghai, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Biotechnol Lett 30:1721-5. 2008
    ..The results showed an immunomodulatory effect and protection against V. mimicus by dietary supplementation of BG. The recommended concentration is 5 g BG/kg diet...
  66. pmc Bioprocess development for the production of mouse-human chimeric anti-epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells
    Suwen Hu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P O Box 329, 130 Meilong Rd, 200237, Shanghai, People s Republic of China
    Cytotechnology 63:247-58. 2011
    ..Therefore, perfusion culture appears to be the optimal process for small-scale production of C12 antibody by rCHO-C12 cells...
  67. ncbi request reprint Enhancement of inosine production by Bacillus subtilis through suppression of carbon overflow by sodium citrate
    Shuangxi Chen
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P O Box 329, 200237, Shanghai, P R China
    Biotechnol Lett 27:689-92. 2005
    ..The phosphoenolpyruvate (PEP) pool was increased by 2.3-fold, while the pyruvate and acetate pools were decreased by 78% and 57%, respectively. Pyruvate kinase might thus be a regulatory site for inosine synthesis...
  68. ncbi request reprint Multi-scale methodology: a key to deciphering systems biology
    Xinhao Ye
    State Key Laboratory of Bioreactor Engineering, National Engineer Research Center for Biotechnology, Department of Biochemical Engineering, East China University of Science and Technology, Shanghai 200237, China PR
    Front Biosci 10:961-5. 2005
    ....
  69. ncbi request reprint [Expression of recombinant human serum albumin in genetically engineered Pichia pastoris in high-density fermentation]
    Meijin Guo
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Wei Sheng Wu Xue Bao 42:62-8. 2002
    ..7841e0.2319t (r = 0.9936); The cell dry weight of broth reached 115-160 g/L and the maximum rHSA concentration was 3.6 g/L at the 120th at the fed-batch fermentation phase...