Yung H Wong

Summary

Affiliation: Clear Water Bay
Country: China

Publications

  1. pmc A molecular and chemical perspective in defining melatonin receptor subtype selectivity
    King Hang Chan
    Biotechnology Research Institute, State Key Laboratory of Molecular Neuroscience, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong
    Int J Mol Sci 14:18385-406. 2013
  2. pmc Gβγ-mediated activation of protein kinase D exhibits subunit specificity and requires Gβγ-responsive phospholipase Cβ isoforms
    Winnie Wi Lau
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Commun Signal 11:22. 2013
  3. pmc Epidermal growth factor differentially augments G(i)-mediated stimulation of c-Jun N-terminal kinase activity
    Anthony S L Chan
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Br J Pharmacol 142:635-46. 2004
  4. pmc Mutations on the Switch III region and the alpha3 helix of Galpha16 differentially affect receptor coupling and regulation of downstream effectors
    May Ym Yu
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China
    J Mol Signal 3:17. 2008
  5. ncbi request reprint Protocatechuic acid induces cell death in HepG2 hepatocellular carcinoma cells through a c-Jun N-terminal kinase-dependent mechanism
    E C H Yip
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Biol Toxicol 22:293-302. 2006
  6. ncbi request reprint Constitutively active Galpha16 stimulates STAT3 via a c-Src/JAK- and ERK-dependent mechanism
    Rico K H Lo
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 278:52154-65. 2003
  7. ncbi request reprint Activation of nuclear factor {kappa}B by somatostatin type 2 receptor in pancreatic acinar AR42J cells involves G{alpha}14 and multiple signaling components: a mechanism requiring protein kinase C, calmodulin-dependent kinase II, ERK, and c-Src
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 280:34617-25. 2005
  8. doi request reprint Prostacyclin receptor-induced STAT3 phosphorylation in human erythroleukemia cells is mediated via Galpha(s) and Galpha(16) hybrid signaling
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 20:2095-106. 2008
  9. ncbi request reprint G16-mediated activation of nuclear factor kappaB by the adenosine A1 receptor involves c-Src, protein kinase C, and ERK signaling
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 279:53196-204. 2004
  10. ncbi request reprint Integration of G protein signals by extracellular signal-regulated protein kinases in SK-N-MC neuroepithelioma cells
    Anthony S L Chan
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Neurochem 94:1457-70. 2005

Collaborators

  • Angel Y F Kam
  • Jing Zhu
  • Yao Tong
  • Calvin B Harley
  • H Y A Lau
  • Y K Tam
  • Stephanie Lee
  • Anthony S L Chan
  • Rico K H Lo
  • Prudence H Tso
  • Lisa Y Yung
  • Eddy H T Wu
  • Maurice K C Ho
  • Andrew M F Liu
  • Maggie M K Lee
  • Yingchun Wang
  • David C New
  • Yueqing Hu
  • Nancy Y Ip
  • Wendy W S Yeung
  • Cecilia S S Wong
  • Dawna H T Kwan
  • Lydia S W Poon
  • Haihong Pang
  • Helen Wise
  • Jessie W F Yuen
  • King H Chan
  • Richard D Ye
  • Grace P W Chan
  • Andrew Mf Liu
  • Yan Su
  • Winnie Wi Lau
  • King Hang Chan
  • Hao Zuo
  • Sejal M Mody
  • Winnie W I Lau
  • Hua Mei
  • Jasmine H P Chan
  • Zhenguo Wu
  • Maurice Kc Ho
  • Yu Pong Ng
  • Andy M F Liu
  • May Ym Yu
  • Guangmiao Fu
  • Frank P L Lai
  • Eric C H Yip
  • E C H Yip
  • Anjali Hazari
  • Shiu F Pang
  • Yaji Tian
  • Kenneth W L Kam
  • Lydia Sw Poon
  • Hermann Ammer
  • Anthony Sl Chan
  • Ricky K S Chui
  • Fanny C F Ip
  • Johnson K Y Ng
  • Allison C Chin
  • Alaster H Y Lau
  • Issan Y S Tam
  • Wing See Lam
  • Emily X Guo
  • Rico Kh Lo
  • Sivia Y S Wong
  • Frances W Y Yu
  • Karl W K Tsim
  • Christina J Morris
  • Jowie C H Yu
  • Becky H L Tam
  • A S L Chan
  • Christina Morris
  • H Pang
  • Sharon C W Luk
  • Patrick M Slocombe
  • Rodger A Allen
  • Breda M Twomey
  • Vicki Lowes
  • Ivy Y W So
  • Ping Y Law
  • Wai Man Wu
  • Joy S C Chan
  • Siu T Tsim
  • Anson H M Pau
  • Helen Cheung
  • Peter N N Ho
  • Horace H Loh
  • Eric J Stanbridge
  • Jason Y M Kam
  • Celia S Pang
  • Tatyana A Voyno-Yasenetskaya

Detail Information

Publications72

  1. pmc A molecular and chemical perspective in defining melatonin receptor subtype selectivity
    King Hang Chan
    Biotechnology Research Institute, State Key Laboratory of Molecular Neuroscience, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong
    Int J Mol Sci 14:18385-406. 2013
    ..The use of homology modeling in the mapping of the ligand-binding pocket will be described. Identification of conserved and distinct residues will be tremendously useful in the design of highly selective ligands...
  2. pmc Gβγ-mediated activation of protein kinase D exhibits subunit specificity and requires Gβγ-responsive phospholipase Cβ isoforms
    Winnie Wi Lau
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Commun Signal 11:22. 2013
    ..However, the relative contribution of these two apparently independent events to Gβγ-mediated PKD activation has yet to be addressed...
  3. pmc Epidermal growth factor differentially augments G(i)-mediated stimulation of c-Jun N-terminal kinase activity
    Anthony S L Chan
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Br J Pharmacol 142:635-46. 2004
    ..6. Our results support a mechanistic model where EGF signaling may differentially regulate the JNK activities triggered by GPCRs of different coupling specificities...
  4. pmc Mutations on the Switch III region and the alpha3 helix of Galpha16 differentially affect receptor coupling and regulation of downstream effectors
    May Ym Yu
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China
    J Mol Signal 3:17. 2008
    ..Cognate residues in Galpha16 might also be essential for interacting with PLCbeta, and possibly contribute to TPR1 interaction and other signaling events...
  5. ncbi request reprint Protocatechuic acid induces cell death in HepG2 hepatocellular carcinoma cells through a c-Jun N-terminal kinase-dependent mechanism
    E C H Yip
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Biol Toxicol 22:293-302. 2006
    ..Collectively, our results suggest that PCA is a naturally occurring compound capable of inducing JNK-dependent hepatocellular carcinoma cell death...
  6. ncbi request reprint Constitutively active Galpha16 stimulates STAT3 via a c-Src/JAK- and ERK-dependent mechanism
    Rico K H Lo
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 278:52154-65. 2003
    ..Demonstration of the involvement of different kinases in Galpha16QL-induced STAT3 activation supports the involvement of multiple signaling pathways in the regulation of transcription by G proteins...
  7. ncbi request reprint Activation of nuclear factor {kappa}B by somatostatin type 2 receptor in pancreatic acinar AR42J cells involves G{alpha}14 and multiple signaling components: a mechanism requiring protein kinase C, calmodulin-dependent kinase II, ERK, and c-Src
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 280:34617-25. 2005
    ..Similar results were also obtained using AR42J cells. These data suggest that activation of the IKK/NFkappaB signaling cascade by SSTR2 requires a complicated network consisting of Galpha(14) and multiple intermediates...
  8. doi request reprint Prostacyclin receptor-induced STAT3 phosphorylation in human erythroleukemia cells is mediated via Galpha(s) and Galpha(16) hybrid signaling
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 20:2095-106. 2008
    ..Taken together, our observations illustrate that co-stimulation of G(s) and G(q) can result in the fine-tuning of STAT3 activation status, and this may provide the basis for cell type-specific responses following activation of hIP...
  9. ncbi request reprint G16-mediated activation of nuclear factor kappaB by the adenosine A1 receptor involves c-Src, protein kinase C, and ERK signaling
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 279:53196-204. 2004
    ..Similar results were obtained using Reh cells. These data suggest that the G(16)-mediated activation of IKK/NFkappaB by CHA required a complex signaling network composed of multiple intermediates...
  10. ncbi request reprint Integration of G protein signals by extracellular signal-regulated protein kinases in SK-N-MC neuroepithelioma cells
    Anthony S L Chan
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Neurochem 94:1457-70. 2005
    ..This report provides evidence that G protein signals can be integrated at the level of MAPK, resulting in differential effects on ERK, JNK and p38 MAPK in SK-N-MC cells...
  11. ncbi request reprint Phosphatidylinositol-3 kinase is distinctively required for mu-, but not kappa-opioid receptor-induced activation of c-Jun N-terminal kinase
    Angel Y F Kam
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Neurochem 89:391-402. 2004
    ..Like the delta-opioid receptor, activation of JNK by the kappa-opioid receptor occurred in a PI3K-independent manner. These studies revealed that the mu-opioid receptor utilize a distinct mechanism to regulate JNK...
  12. ncbi request reprint Transcriptional activation of c-Fos by constitutively active Galpha(16)QL through a STAT1-dependent pathway
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 18:2143-53. 2006
    ..Collectively, our results demonstrate for the first time that stimulation of Galpha(16) can lead to STAT1-dependent c-Fos transcriptional activation via PLCbeta, c-Src/JAK and ERK pathways...
  13. ncbi request reprint Prostacyclin receptor induces STAT1 and STAT3 phosphorylations in human erythroleukemia cells: a mechanism requiring PTX-insensitive G proteins, ERK and JNK
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 18:307-17. 2006
    ..Taken together, these results demonstrate for the first time that hIP activation by cicaprost can lead to STAT1 and STAT3 phosphorylations via signaling pathways involving PTX-insensitive G proteins, ERK and JNK...
  14. doi request reprint Multiple Gi proteins participate in nerve growth factor-induced activation of c-Jun N-terminal kinases in PC12 cells
    Prudence H Tso
    Department of Biochemistry, the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Neurochem Res 34:1101-12. 2009
    ..Collectively, these studies indicate that NGF-dependent JNK activity may be mediated via G(i1-3) proteins, JAK3, Src, p38 MAPK and the MEK/ERK cascade...
  15. ncbi request reprint The beta6/alpha5 regions of Galphai2 and GalphaoA increase the promiscuity of Galpha16 but are insufficient for pertussis toxin-catalyzed ADP-ribosylation
    Cecilia S S Wong
    Department of Biochemistry, Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Eur J Pharmacol 473:105-15. 2003
    ..Although incorporation of a Galphai-like beta6/alpha5 region into the C-terminus of Galpha16 increases its promiscuity, this region is not sufficient to support recognition by pertussis toxin...
  16. ncbi request reprint Gbetagamma signaling and Ca2+ mobilization co-operate synergistically in a Sos and Rac-dependent manner in the activation of JNK by Gq-coupled receptors
    Anthony S L Chan
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 16:823-36. 2004
    ..We propose that the co-operative effect between Gbetagamma-mediated signaling and the increased intracellular Ca(2+) level represents a robust mechanism for the stimulation of JNK by G(q)-coupled receptors...
  17. ncbi request reprint Pertussis toxin-sensitive Gi/o proteins are involved in nerve growth factor-induced pro-survival Akt signaling cascade in PC12 cells
    Eddy H T Wu
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 17:881-90. 2005
    ..This is the first study that demonstrates the involvement of G(i/o) proteins in NGF-induced Akt signaling...
  18. ncbi request reprint Regulation of STAT3 by mu-opioid receptors in human neuroblastoma SH-SY5Y cells
    Jessie W F Yuen
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Neuroreport 15:1431-5. 2004
    ..The present study provides a foundation to explore the importance of STAT3 signaling in the regulation of neuronal growth and differentiation by the mu-opioid receptor...
  19. ncbi request reprint Replacement of the alpha5 helix of Galpha16 with Galphas-specific sequences enhances promiscuity of Galpha16 toward Gs-coupled receptors
    Anjali Hazari
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 16:51-62. 2004
    ..Similar results were observed for agonist-induced Ca(2+) mobilizations. These results show that incorporating the alpha5 helix of Galpha(s) into Galpha(16) can increase the promiscuity of 16s25 towards G(s)-coupled receptors...
  20. ncbi request reprint Activation of STAT3 by G alpha(s) distinctively requires protein kinase A, JNK, and phosphatidylinositol 3-kinase
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 281:35812-25. 2006
    ..However, unlike the mechanisms employed by Galpha(i) and Galpha(14/16), Galpha(s) distinctively requires protein kinase A, JNK, and phosphatidylinositol 3-kinase for STAT3 activation...
  21. ncbi request reprint Activation of muscarinic M4 receptor augments NGF-induced pro-survival Akt signaling in PC12 cells
    Eddy H T Wu
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 18:285-93. 2006
    ..This is the first study that demonstrates the augmentation effect between M4 mAChR and NGF receptor, and the regulatory role of mAChR on tuberin...
  22. doi request reprint Astragaloside IV and cycloastragenol stimulate the phosphorylation of extracellular signal-regulated protein kinase in multiple cell types
    Lisa Y Yung
    Biotechnology Research Institute and Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Planta Med 78:115-21. 2012
    ..Our data indicate that AG-IV and CAG may exert their cellular effects through the activation of the Src/MEK/ERK pathway...
  23. ncbi request reprint Signal transducer and activator of transcription 3 activation by the delta-opioid receptor via Galpha14 involves multiple intermediates
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Mol Pharmacol 65:1427-39. 2004
    ..These results demonstrate for the first time that Galpha14 activation can lead to STAT3 stimulation via a complex signaling network involving multiple intermediates...
  24. ncbi request reprint Kappa-opioid receptor signals through Src and focal adhesion kinase to stimulate c-Jun N-terminal kinases in transfected COS-7 cells and human monocytic THP-1 cells
    Angel Y F Kam
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Pharmacol Exp Ther 310:301-10. 2004
    ..These studies demonstrate that the activation of JNK by kappa-opioid receptors is routed via Gbetagamma, Src, FAK, Sos, Rac, and Cdc42...
  25. doi request reprint RGS19 inhibits Ras signaling through Nm23H1/2-mediated phosphorylation of the kinase suppressor of Ras
    Prudence H Tso
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Signal 25:1064-74. 2013
    ..This study demonstrates that expression of RGS19 can suppress Ras-mediated signaling via upregulation of Nm23...
  26. ncbi request reprint Characterization of substituted phenylpropylamides as highly selective agonists at the melatonin MT2 receptor
    King H Chan
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Curr Med Chem 20:289-300. 2013
    ..These ligands represent invaluable tools for delineating the functional roles of distinct melatonin receptor subtypes and are viable candidates for drug development...
  27. doi request reprint CCR1-mediated STAT3 tyrosine phosphorylation and CXCL8 expression in THP-1 macrophage-like cells involve pertussis toxin-insensitive Gα(14/16) signaling and IL-6 release
    Maggie M K Lee
    Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Immunol 189:5266-76. 2012
    ....
  28. doi request reprint Activation of Ras-dependent signaling pathways by G(14) -coupled receptors requires the adaptor protein TPR1
    Dawna H T Kwan
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Cell Biochem 113:3486-97. 2012
    ..Our results suggest that TPR1 is required for Gα(14) to stimulate Ras-dependent signaling pathways, but not for the propagation of signals along Ras-independent pathways...
  29. ncbi request reprint Formyl peptide-receptor like-1 requires lipid raft and extracellular signal-regulated protein kinase to activate inhibitor-kappa B kinase in human U87 astrocytoma cells
    Angel Y F Kam
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China
    J Neurochem 103:1553-66. 2007
    ..Taken together, these results demonstrate that FPRL-1 is capable of activating NFkappaB signaling through IKK phosphorylation and this may serve as a useful therapeutical target for FPRL-1-related diseases...
  30. ncbi request reprint Formyl peptide receptor like 1 differentially requires mitogen-activated protein kinases for the induction of glial fibrillary acidic protein and interleukin-1alpha in human U87 astrocytoma cells
    Angel Y F Kam
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 19:2106-17. 2007
    ..We have thus identified a possible linkage among FPRL1, MAPKs, astrocytic activation and the inflammatory response...
  31. ncbi request reprint The first and third intracellular loops together with the carboxy terminal tail of the delta-opioid receptor contribute toward functional interaction with Galpha16
    Anthony S L Chan
    Department of Biochemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China
    J Neurochem 87:697-708. 2003
    ..These results indicate that efficient coupling of the delta-opioid receptor to Galpha16 requires the participation of most of the intracellular regions, including the first intracellular loop...
  32. doi request reprint Nerve growth factor-induced stimulation of p38 mitogen-activated protein kinase in PC12 cells is partially mediated via G(i/o) proteins
    Lisa Y Yung
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 20:1538-44. 2008
    ..Collectively, these studies indicate that NGF-dependent p38 MAPK activity may be mediated via G(i2) protein, Src, and the MEK/ERK cascade...
  33. doi request reprint Activation of STAT3 by specific Galpha subunits and multiple Gbetagamma dimers
    Jessie W F Yuen
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Int J Biochem Cell Biol 42:1052-9. 2010
    ..These results suggested that the activation of STAT family members by Galpha(16) or Galpha(14) was selective and that distinct combinations of Gbetagamma complexes can also regulate the STAT signaling pathway...
  34. ncbi request reprint Differential chemokine activation of CC chemokine receptor 1-regulated pathways: ligand selective activation of Galpha 14-coupled pathways
    Yaji Tian
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China
    Eur J Immunol 34:785-95. 2004
    ..CCR1 and Galpha14 or Galpha16 are co-expressed in several cell types and we hypothesize that selective activation of chemokine receptors provides a mechanism by which chemokines are able to fine-tune intracellular signaling pathways...
  35. doi request reprint Regulation of mTOR and p70 S6 kinase by the muscarinic M4 receptor in PC12 cells
    Grace P W Chan
    Department of Biochemistry, the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Biol Int 33:230-8. 2009
    ..Collectively, these findings suggest that PP2A and PTEN may be involved in fine tuning the regulation of Akt/tuberin/mTOR/p70S6K in PC12 cells by M(4) mAChR and TrkA, respectively...
  36. ncbi request reprint Regulation of STAT3 activity by G16-coupled receptors
    Eddy H T Wu
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Biochem Biophys Res Commun 303:920-5. 2003
    ..This is the first study that demonstrates the stimulatory effect of ORL(1) and fMLP receptors on STAT3 activity...
  37. ncbi request reprint Molecular determinants for the differential coupling of Galpha(16) to the melatonin MT1, MT2 and Xenopus Mel1c receptors
    Frank P L Lai
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Neurochem 80:736-45. 2002
    ..Our results suggest that Galpha(16) can discern structural differences amid the three melatonin receptors and provide evidence for functional distinction of Mel1c from MT1 and MT2 receptors...
  38. doi request reprint Galpha16 activates Ras by forming a complex with tetratricopeptide repeat 1 (TPR1) and Son of Sevenless (SOS)
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 22:1448-58. 2010
    ..Collectively, our results suggest that Galpha16 regulates multiple signaling pathways by activating Ras through its association with TPR1, but TPR1 is not required for Galpha16 to stimulate phospholipase Cbeta...
  39. pmc Identification of a stretch of six divergent amino acids on the alpha5 helix of Galpha16 as a major determinant of the promiscuity and efficiency of receptor coupling
    Maurice K C Ho
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People s Republic of China
    Biochem J 380:361-9. 2004
    ....
  40. ncbi request reprint The aqueous extract of Radix Glycyrrhizae stimulates mitogen-activated protein kinases and nuclear factor-kappaB in Jurkat T-cells and THP-1 monocytic cells
    Anthony S L Chan
    Department of Biochemistry, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China
    Am J Chin Med 34:263-78. 2006
    ....
  41. ncbi request reprint Activation of delta-, kappa-, and mu-opioid receptors induces phosphorylation of tuberin in transfected HEK 293 cells and native cells
    Eddy H T Wu
    Department of Biochemistry, the Molecular Neuroscience Center, The Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Biochem Biophys Res Commun 334:838-44. 2005
    ..This is the first study that demonstrates the regulatory role of opioid receptors on tuberin...
  42. ncbi request reprint Opioid receptor-like (ORL1) receptor utilizes both G(oA) and G(oB) for signal transduction
    Prudence H Tso
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Protein Pept Lett 13:437-41. 2006
    ..However, development of AC superactivation and loss of ERK1/2 responsiveness induced by chronic activation of the ORL1 receptors remained PTX-sensitive...
  43. ncbi request reprint Melatonin mt1 and MT2 receptors stimulate c-Jun N-terminal kinase via pertussis toxin-sensitive and -insensitive G proteins
    Anthony S L Chan
    Department of Biochemistry the Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 14:249-57. 2002
    ..This report demonstrates the stimulatory effect of melatonin receptors on JNK, and provides experimental evidence for a functional coupling between the G(i)-coupled melatonin receptor and Gs, in terms of adenylyl cyclase activation...
  44. pmc Activation state-dependent interaction between Gαq subunits and the Fhit tumor suppressor
    Hao Zuo
    Division of Life Sciences, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Commun Signal 11:59. 2013
    ..Since many G proteins can regulate cell proliferation through multiple signaling components including Src, we explored the relationship between Gα subunits and Fhit...
  45. ncbi request reprint Rac and Cdc42-dependent regulation of c-Jun N-terminal kinases by the delta-opioid receptor
    Angel Y F Kam
    Department of Biochemistry, the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Neurochem 84:503-13. 2003
    ..Collectively, these results demonstrate functional regulation of JNK by the delta-opioid receptor, and this pathway requires Gbetagamma, Src kinases and the small GTPases Rac and Cdc42...
  46. doi request reprint RGS19 stimulates cell proliferation by deregulating cell cycle control and enhancing Akt signaling
    Prudence H Tso
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cancer Lett 309:199-208. 2011
    ..Consistent with an elevated Akt activity, increased levels of phosphorylated Bad and c-Raf and a diminished expression of TSC2 were detected, thus demonstrating that RGS19 can deregulate cell proliferation via multiple pathways...
  47. doi request reprint Reporter gene assays
    Andy M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China
    Methods Mol Biol 486:109-23. 2009
    ....
  48. ncbi request reprint Gq-mediated activation of c-Jun N-terminal kinase by the gastrin-releasing peptide-preferring bombesin receptor is inhibited upon costimulation of the Gs-coupled dopamine D1 receptor in COS-7 cells
    Anthony S L Chan
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Mol Pharmacol 68:1354-64. 2005
    ....
  49. doi request reprint RGS19 enhances cell proliferation through its C-terminal PDZ motif
    Prudence H Tso
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Signal 22:1700-7. 2010
    ..This study demonstrates that RGS19, in addition to acting as a GAP, is able to stimulate cell proliferation in a GIPC-dependent manner...
  50. ncbi request reprint Constitutive activation of the opioid receptor-like (ORL1) receptor by mutation of Asn133 to tryptophan in the third transmembrane region
    Kenneth W L Kam
    Department of Biochemistry, Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clearwater Bay, Hong Kong, China
    J Neurochem 83:1461-70. 2002
    ..In conclusion, our results demonstrate that a mutation in the third transmembrane domain is able to increase the basal signalling activity of the human ORL1 receptor...
  51. ncbi request reprint Involvement of G i/o proteins in nerve growth factor-stimulated phosphorylation and degradation of tuberin in PC-12 cells and cortical neurons
    Eddy H T Wu
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Mol Pharmacol 67:1195-205. 2005
    ..Together, this study demonstrates the regulatory effect of NGF and G(i/o) signaling on tuberin...
  52. ncbi request reprint Activation of the human FPRL-1 receptor promotes Ca2+ mobilization in U87 astrocytoma cells
    Dawna H T Kwan
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Neurochem Res 33:125-33. 2008
    ..Our data demonstrated that activation of Gi-coupled FPRL-1 can lead to Ca2+ influx possibly via SOCs in U87 and FPRL-1/CHO cells...
  53. ncbi request reprint Constitutively active alpha subunits of G(q/11) and G(12/13) families inhibit activation of the pro-survival Akt signaling cascade
    Eddy H T Wu
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    FEBS J 273:2388-98. 2006
    ..Collectively, this study demonstrated the inhibitory effect of activated G alpha11, G alpha14, G alpha16, G alpha12 and G alpha13 on pro-survival Akt signaling...
  54. doi request reprint Synthesis of substituted N-[3-(3-methoxyphenyl)propyl] amides as highly potent MT(2)-selective melatonin ligands
    Yueqing Hu
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Centre, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Bioorg Med Chem Lett 20:2582-5. 2010
    ..It was discovered that a benzyloxyl substituent incorporated at C6 position of the 3-methoxyphenyl ring dramatically enhanced the MT(2) binding affinity and at the same time decreased MT(1) binding affinity...
  55. doi request reprint Interleukin-6 autocrine signaling mediates melatonin MT(1/2) receptor-induced STAT3 Tyr(705) phosphorylation
    Winnie W I Lau
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Pineal Res 52:477-89. 2012
    ..Our results demonstrate that melatonin receptor/Gα(16) coupling is capable of triggering the production of cytokines including IL-6, and this autocrine loop may account for the subsequent STAT3 phosphorylation at Tyr(705) ...
  56. pmc Gα16 interacts with tetratricopeptide repeat 1 (TPR1) through its β3 region to activate Ras independently of phospholipase Cβ signaling
    Andrew Mf Liu
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    BMC Struct Biol 11:17. 2011
    ..Using Gα16 as a model, we asked if its recently discovered adaptor protein tetratricopeptide repeat 1 (TPR1) binds to the same region as its canonical effector, phospholipase Cβ (PLCβ)...
  57. ncbi request reprint CKBM stimulates MAPKs but inhibits LPS-induced IFN-gamma in lymphocytes
    Anthony S L Chan
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Phytother Res 20:725-31. 2006
    ....
  58. ncbi request reprint Mu-opioid receptor-mediated phosphorylation of IkappaB kinase in human neuroblastoma SH-SY5Y cells
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Kowloon, SAR, China
    Neurosignals 14:136-42. 2005
    ..These data suggest that the mu-opioid receptor is capable of simulating NFkappaB signaling via the phosphorylation of IKK and p65 in human neuroblastoma SH-SY5Y cells...
  59. doi request reprint CCR1-mediated activation of Nuclear Factor-kappaB in THP-1 monocytic cells involves Pertussis Toxin-insensitive Galpha(14) and Galpha(16) signaling cascades
    Maggie M K Lee
    Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    J Leukoc Biol 86:1319-29. 2009
    ..The ability of CCR1 to signal through Galpha(14/16) thus provides a linkage for chemokines to regulate NF-kappaB-dependent responses...
  60. doi request reprint Gbeta3 forms distinct dimers with specific Ggamma subunits and preferentially activates the beta3 isoform of phospholipase C
    Lydia S W Poon
    Department of Biochemistry, Biotechnology Research Institute, and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Signal 21:737-44. 2009
    ....
  61. doi request reprint Regulator of G protein signaling 19 suppresses Ras-induced neoplastic transformation and tumorigenesis
    Yingchun Wang
    Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cancer Lett 339:33-41. 2013
    ..Collectively, these results suggest that expression of RGS19 can suppress the oncogenic actions of Ras...
  62. doi request reprint 3-Methoxylphenylpropyl amides as novel receptor subtype-selective melatoninergic ligands: characterization of physicochemical and pharmacokinetic properties
    Jing Zhu
    Section of Biochemistry and Cell Biology, Division of Life Sciences, and the Biotechnology Research Institute, Clear Water Bay, Kowloon, Hong Kong
    Xenobiotica 41:35-45. 2011
    ..Metabolite profiling unveiled that C6-ether linkage and methoxy substituents were likely the major metabolic soft spots in their structures, which provided important information for further improvement of their structural stability...
  63. doi request reprint Differential and synergistic effect of nerve growth factor and cAMP on the regulation of early response genes during neuronal differentiation
    Yu Pong Ng
    Department of Biochemistry, Biotechnology Research Institute and Molecular Neuroscience Center, Hong Kong, SAR, China
    Neurosignals 17:111-20. 2009
    ..Our observations provide novel insights on the signalling mechanisms underlying the regulation of neuronal differentiation by NGF and cAMP...
  64. ncbi request reprint Galpha(16/z) chimeras efficiently link a wide range of G protein-coupled receptors to calcium mobilization
    Andrew M F Liu
    Department of Biochemisty, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Biomol Screen 8:39-49. 2003
    ..Collectively, these studies help to validate the promiscuity of the Galpha(16/z) chimeras as well as their application in contemporary drug-screening assays that are based on ligand-induced Ca(2+) mobilization...
  65. doi request reprint A hematopoietic perspective on the promiscuity and specificity of Galpha16 signaling
    Yan Su
    Biotechnology Research Institute, Molecular Neuroscience Center, Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong, SAR, China
    Neurosignals 17:71-81. 2009
    ..This review aims to provide an updated appreciation and rational discussion of Galpha(16) signaling with regard to its promiscuity and specificity...
  66. ncbi request reprint In vitro intestinal absorption and first-pass intestinal and hepatic metabolism of cycloastragenol, a potent small molecule telomerase activator
    Jing Zhu
    Biotechnology Research Institute, Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong, China
    Drug Metab Pharmacokinet 25:477-86. 2010
    ..The present study indicates that CAG is efficiently absorbed through intestinal epithelium. However, extensive first-pass hepatic metabolism would limit the oral bioavailability of this compound...
  67. ncbi request reprint In search of novel and therapeutically significant melatoninergic ligands
    Sejal M Mody
    Department of Biochemistry and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Recent Pat CNS Drug Discov 2:241-5. 2007
    ..In this review an overview of recently developed novel melatoninergic ligands is provided including recently filed patents and compounds undergoing clinical evaluation...
  68. doi request reprint The RhoA-specific guanine nucleotide exchange factor p63RhoGEF binds to activated Galpha(16) and inhibits the canonical phospholipase Cbeta pathway
    Wendy W S Yeung
    Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Cell Signal 21:1317-25. 2009
    ..Taken together, these results suggest that p63RhoGEF binds to activated Galpha(16) and inhibits its signaling pathways...
  69. ncbi request reprint Naturally occurring phenylethanoid glycosides: potential leads for new therapeutics
    Guangmiao Fu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    Curr Med Chem 15:2592-613. 2008
    ..Particular attention will be given to the novel structures identified to date and the prominent therapeutic values associated with these molecules...
  70. ncbi request reprint The ORL1 receptor: molecular pharmacology and signalling mechanisms
    David C New
    Department of Biochemistry, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    Neurosignals 11:197-212. 2002
    ....
  71. doi request reprint Expression of Gα(z) in C2C12 cells restrains myogenic differentiation
    Hua Mei
    Section of Biochemistry and Cell Biology, Division of Life Science, Biotechnology Research Institute, Molecular Neuroscience Center, The State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 23:389-97. 2011
    ..These results support a novel role of Gα(z) in restraining myogenic differentiation through the disruption of Rho signaling...
  72. ncbi request reprint G protein-linked effector and second messenger systems involved in melatonin signal transduction
    David C New
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong, China
    Neurosignals 12:59-70. 2003
    ..This review will attempt to summarize recent research by many groups that has revealed numerous subtleties of the melatonin-coupled signaling pathways...