Affiliation: Clear Water Bay
Qin C, Zhong X, Bu X, Ng N, Guo Z. Dissociation of antibacterial and hemolytic activities of an amphipathic peptide antibiotic. J Med Chem. 2003;46:4830-3 pubmed
..Successful dissociation of the two intimately associated properties should enable discovery of novel analogues with both high bacterial selectivity and antibacterial potency to counter microbial resistance. ..
Qin M, Song H, Dai X, Chen Y, Guo Z. Two active site arginines are critical determinants of substrate binding and catalysis in MenD, a thiamine-dependent enzyme in menaquinone biosynthesis. Biochem J. 2018;: pubmed publisher
..More importantly, the nonnative binding site of the inactive MenD intermediate uncovered here provides a new target for development of antibiotics. ..
Sun Y, Yin S, Feng Y, Li J, Zhou J, Liu C, et al
. Molecular basis of the general base catalysis of an ?/?-hydrolase catalytic triad. J Biol Chem. 2014;289:15867-79 pubmed publisher
Chen Y, Sun Y, Song H, Guo Z. Structural Basis for the ATP-dependent Configuration of Adenylation Active Site in Bacillus subtilis o-Succinylbenzoyl-CoA Synthetase. J Biol Chem. 2015;290:23971-83 pubmed publisher
..These findings provide new insights into the adenylation half-reaction in the domain alteration catalytic mechanism of the adenylate-forming enzymes. ..
Chen Y, Li T, Lin X, Li X, Li X, Guo Z. Crystal structure of the thioesterification conformation of Bacillus subtilis o-succinylbenzoyl-CoA synthetase reveals a distinct substrate-binding mode. J Biol Chem. 2017;292:12296-12310 pubmed publisher
..These findings provide new insights into the domain-alternation catalysis of a bacterial enzyme essential for vitamin K biosynthesis and of its adenylating homologues in the ANL enzyme family. ..