Duanqing Pei

Summary

Affiliation: Chinese Academy of Sciences
Country: China

Publications

  1. ncbi request reprint Co-recycling of MT1-MMP and MT3-MMP through the trans-Golgi network. Identification of DKV582 as a recycling signal
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:9331-6. 2004
  2. ncbi request reprint Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation
    Guangjin Pan
    Institute of Pharmacology and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    J Biol Chem 279:37013-20. 2004
  3. ncbi request reprint Matrix metalloproteinases target protease-activated receptors on the tumor cell surface
    Duanqing Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Cancer Cell 7:207-8. 2005
  4. ncbi request reprint Regulation of the pluripotency marker Rex-1 by Nanog and Sox2
    Wenjing Shi
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 281:23319-25. 2006
  5. ncbi request reprint A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal
    Guangjin Pan
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing, China
    FASEB J 20:1730-2. 2006
  6. doi request reprint Regulation of pluripotency and reprogramming by transcription factors
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 284:3365-9. 2009
  7. doi request reprint The magic continues for the iPS strategy
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China
    Cell Res 18:221-3. 2008
  8. ncbi request reprint IUPHAR 2006--15th World Congress of Pharmacology. Metalloproteinases as Therapeutical Targets for Cancer Therapy. 2-7 July 2006, Beijing, China
    Duanqing Pei
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, 100 Middle Xianlie Road C, Guangzhou 510070, China
    IDrugs 9:598-9. 2006
  9. doi request reprint Induced pluripotent stem cell technology in regenerative medicine and biology
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China
    Adv Biochem Eng Biotechnol 123:127-41. 2010
  10. ncbi request reprint The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4
    Huayu Qi
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Cell Res 17:578-80. 2007

Collaborators

Detail Information

Publications80

  1. ncbi request reprint Co-recycling of MT1-MMP and MT3-MMP through the trans-Golgi network. Identification of DKV582 as a recycling signal
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:9331-6. 2004
    ..These results suggest that MT-MMPs may coordinate their proteolytic activities through the cellular trafficking machinery...
  2. ncbi request reprint Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation
    Guangjin Pan
    Institute of Pharmacology and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    J Biol Chem 279:37013-20. 2004
    ..These data suggest that this dominant negative form of OCT4 may be a useful tool for modulating the activity of OCT4 in pluripotent cells such as embryonic stem cells to achieve the desired cell types for therapeutic applications...
  3. ncbi request reprint Matrix metalloproteinases target protease-activated receptors on the tumor cell surface
    Duanqing Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Cancer Cell 7:207-8. 2005
    ..This MMP-PAR axis may represent a novel signaling pathway communicating between tumor and stromal cells during tumor progression...
  4. ncbi request reprint Regulation of the pluripotency marker Rex-1 by Nanog and Sox2
    Wenjing Shi
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 281:23319-25. 2006
    ..Taking these findings together, we conclude that Rex-1 is a direct target of Nanog, which is augmented by Sox2 and Oct-3/4...
  5. ncbi request reprint A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal
    Guangjin Pan
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing, China
    FASEB J 20:1730-2. 2006
    ..Our results suggest the FoxD3-Nanog-Oct4 loop anchors an interdependent network of transcription factors that regulate stem cell pluripotency...
  6. doi request reprint Regulation of pluripotency and reprogramming by transcription factors
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 284:3365-9. 2009
    ..This minireview summarizes recent advances in pluripotency and reprogramming by focusing on key transcription factors and the likely mechanisms...
  7. doi request reprint The magic continues for the iPS strategy
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China
    Cell Res 18:221-3. 2008
  8. ncbi request reprint IUPHAR 2006--15th World Congress of Pharmacology. Metalloproteinases as Therapeutical Targets for Cancer Therapy. 2-7 July 2006, Beijing, China
    Duanqing Pei
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, 100 Middle Xianlie Road C, Guangzhou 510070, China
    IDrugs 9:598-9. 2006
  9. doi request reprint Induced pluripotent stem cell technology in regenerative medicine and biology
    Duanqing Pei
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China
    Adv Biochem Eng Biotechnol 123:127-41. 2010
    ..Here, we discuss some important implications of human iPSCs for biology and medicine and also point to possible future directions...
  10. ncbi request reprint The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4
    Huayu Qi
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Cell Res 17:578-80. 2007
  11. doi request reprint The mighty mice prove pluripotency for iPSCs
    Duanqing Pei
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    J Mol Cell Biol 2:171-2. 2010
    ..e. capable of giving rise to every cell in the newborn mice. This achievement validates iPS technology for regenerative medicine...
  12. ncbi request reprint The hemopexin domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) Is not required for its activation of proMMP2 on cell surface but is essential for MT1-MMP-mediated invasion in three-dimensional type I collagen
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:51148-55. 2004
    ....
  13. pmc Regulation of MT1-MMP activity by β-catenin in MDCK non-cancer and HT1080 cancer cells
    Ping Liu
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Cell Physiol 225:810-21. 2010
    ..These differences were most probably due to different subcellular locations and different involved pathways of β-catenin in these cells...
  14. doi request reprint Increased aggressiveness of human prostate PC-3 tumor cells expressing cell surface localized membrane type-1 matrix metalloproteinase (MT1-MMP)
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Androl 30:259-74. 2009
    ....
  15. doi request reprint Generation of human induced pluripotent stem cells from urine samples
    Ting Zhou
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, China
    Nat Protoc 7:2080-9. 2012
    ..Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney...
  16. doi request reprint Generation of integration-free neural progenitor cells from cells in human urine
    Lihui Wang
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Nat Methods 10:84-9. 2013
    ..Although functional in vivo analysis is still needed, we report that the cells survive and differentiate upon transplant into newborn rat brain...
  17. doi request reprint A new diaryl urea compound, D181, induces cell cycle arrest in the G1 and M phases by targeting receptor tyrosine kinases and the microtubule skeleton
    Jin Zhang
    Key Laboratory of Regenerative Biology and Institute of Chemical Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Road, Guangzhou Science Park, Guangzhou, Guangdong Province 510530, China
    Invest New Drugs 30:490-507. 2012
    ..Our studies have provided a new, promising lead compound and novel clues for multi-target anticancer drug design and development...
  18. pmc Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics
    Jiekai Chen
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine at Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Res 21:884-94. 2011
    ..This new paradigm not only provides a rationale to further improve iPSC generation but also simplifies the conceptual understanding of reprogramming by defined factors...
  19. ncbi request reprint Mint-3 regulates the retrieval of the internalized membrane-type matrix metalloproteinase, MT5-MMP, to the plasma membrane by binding to its carboxyl end motif EWV
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA
    J Biol Chem 279:20461-70. 2004
    ..Furthermore, Mint-3 significantly increased the level of MT5-MMP on the cell surface without affecting its synthesis and internalization. Therefore, Mints may be the adaptor proteins that regulate the trafficking of MT-MMPs...
  20. pmc BMPs functionally replace Klf4 and support efficient reprogramming of mouse fibroblasts by Oct4 alone
    Jiekai Chen
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine at Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Res 21:205-12. 2011
    ..BMPs also promoted single-factor Oct4-based reprogramming of MEFs and tail tibial fibroblasts. Our studies clarify the contribution of Klf4 in reprogramming and establish Oct4 as a singular setter of pluripotency in differentiated cells...
  21. ncbi request reprint Complete restoration of cell surface activity of transmembrane-truncated MT1-MMP by a glycosylphosphatidylinositol anchor. Implications for MT1-MMP-mediated prommp2 activation and collagenolysis in three-dimensions
    Jing Nie
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 282:6438-43. 2007
    ..Our results suggest that both membrane-tethering and proteolytic activity encoded by MT1-MMP are required for its ability to promote cell growth and invasion in a three-dimensional collagen matrix...
  22. doi request reprint A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts
    Ronghui Li
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Stem Cell 7:51-63. 2010
    ..Our work not only establishes MET as a key cellular mechanism toward induced pluripotency, but also demonstrates iPSC generation as a cooperative process between the defined factors and the extracellular milieu. PAPERCLIP:..
  23. doi request reprint Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual factors and a sequential EMT-MET mechanism for optimal reprogramming
    Xiaopeng Liu
    CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Nat Cell Biol 15:829-38. 2013
    ..Our studies provide a rationale for further optimizing reprogramming, and introduce the concept of a sequential EMT-MET mechanism for cell fate decision that should be investigated further in other systems, both in vitro and in vivo...
  24. doi request reprint H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs
    Jiekai Chen
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Nat Genet 45:34-42. 2013
    ..Our results not only establish pre-iPSCs as an epigenetically stable signpost along the reprogramming road map, but they also provide mechanistic insights into the epigenetic reprogramming of cell fate...
  25. doi request reprint MicroRNAs in somatic cell reprogramming
    Xichen Bao
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510530, China
    Curr Opin Cell Biol 25:208-14. 2013
    ..Moreover, variations in microRNA expression of iPS/ES cells can predict their differentiation potential and may have consequences at other levels. Altogether, these findings highlight the relevance of pursuing further these studies...
  26. doi request reprint The mesenchymal-to-epithelial transition in somatic cell reprogramming
    Miguel A Esteban
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510530, China
    Curr Opin Genet Dev 22:423-8. 2012
    ..Further understanding of the molecular events controlling both situations has relevant implications for regenerative medicine and disease...
  27. doi request reprint Modeling abnormal early development with induced pluripotent stem cells from aneuploid syndromes
    Wen Li
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Hum Mol Genet 21:32-45. 2012
    ..These data support that abnormal organogenesis and early lethality in TS are not caused by a tissue-specific differentiation blockade, but rather involves other abnormalities including impaired placentation...
  28. doi request reprint Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells
    Huanxing Su
    Stem Cell and Cancer Biology Group, Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Stem Cell Res 10:338-48. 2013
    ..Our results revealed a critical role of Cdh2 in the process of efficient neural differentiation of mouse iPS cells...
  29. ncbi request reprint Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1
    Michael J Wilson
    Minneapolis VA Medical Center, and Department of Laboratory Medicine and Pathology, and Minnesoata Cancer Center, University of Minnesota, Minneapolis, USA
    J Androl 25:274-85. 2004
    ..The molecular basis for the low level of processing of pro-MMP-2 by PC-3 cells may be due to an overabundance of TIMP-2 and/or a low level of cell surface active MT1-MMP...
  30. pmc Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
    Jinglei Cai
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China
    J Biol Chem 285:11227-34. 2010
    ....
  31. pmc Class IIa histone deacetylases and myocyte enhancer factor 2 proteins regulate the mesenchymal-to-epithelial transition of somatic cell reprogramming
    Qiang Zhuang
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510530, China and
    J Biol Chem 288:12022-31. 2013
    ..Our work highlights an unexpected role for a developmental axis in somatic cell reprogramming and provides new insight into how the MET is regulated in this context...
  32. doi request reprint The histone demethylases Jhdm1a/1b enhance somatic cell reprogramming in a vitamin-C-dependent manner
    Tao Wang
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Stem Cell 9:575-87. 2011
    ..Our results therefore reveal a role for H3K36me2/3 in cell fate determination and establish a link between histone demethylases and vitamin-C-induced reprogramming...
  33. pmc Generation of induced pluripotent stem cell lines from Tibetan miniature pig
    Miguel A Esteban
    Stem Cell and Cancer Biology Group, Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510663, USA
    J Biol Chem 284:17634-40. 2009
    ..Because porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutic applications of iPS in a large animal model...
  34. ncbi request reprint Membrane type-1 matrix metalloproteinase promotes human melanoma invasion and growth
    Joji Iida
    Department of Laboratory Medicine and Pathology, and University of Minnesota Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Invest Dermatol 122:167-76. 2004
    ..The results suggest a more general role for elevated MT1-MMP in promoting both the selective invasion and increased growth of malignant melanoma in vivo...
  35. pmc Induced pluripotent stem cells can be used to model the genomic imprinting disorder Prader-Willi syndrome
    Jiayin Yang
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    J Biol Chem 285:40303-11. 2010
    ..These results indicate that iPSCs may be a useful tool to study PWS and perhaps other genetic imprinting diseases as well...
  36. pmc Structure of sorting nexin 11 (SNX11) reveals a novel extended phox homology (PX) domain critical for inhibition of SNX10-induced vacuolation
    Jinxin Xu
    State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    J Biol Chem 288:16598-605. 2013
    ..We propose that this PXe domain is present in SNX10 and is responsible for the vacuolation activity of SNX10. Thus, this novel PXe domain constitutes a structurally and functionally important PX domain subfamily...
  37. doi request reprint Pyrimido[4,5-d]pyrimidin-4(1H)-one Derivatives as Selective Inhibitors of EGFR Threonine(790) to Methionine(790) (T790M) Mutants
    Tianfeng Xu
    Institute of Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Guangzhou 510530 China University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049 China
    Angew Chem Int Ed Engl 52:8387-90. 2013
    ..They also selectively inhibited EGFR signal transduction and proliferation of NSCLC cells harboring EGFR(L858R/T790M) mutation, but were significantly less potent to cells with EGFR(WT) . ..
  38. doi request reprint EMT and MET as paradigms for cell fate switching
    Jiekai Chen
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine at Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    J Mol Cell Biol 4:66-9. 2012
    ..As such, we believe that investigations into MET-EMT may yield detailed molecular insights into cell fate decisions, not only for the switching between epithelial and mesenchymal cells, but also other cell types...
  39. doi request reprint Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells
    Xiaofei Zhang
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 283:35825-33. 2008
    ..In summary, our experiments help to understand how Esrrb coordinates with Nanog and Oct4 to activate the internal machinery of ES cells...
  40. doi request reprint Vitamin C enhances the generation of mouse and human induced pluripotent stem cells
    Miguel Angel Esteban
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    Cell Stem Cell 6:71-9. 2010
    ..Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process...
  41. doi request reprint Urine as a source of stem cells
    Christina Benda
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, 510530, China
    Adv Biochem Eng Biotechnol 129:19-32. 2013
    ..In addition, recent work including ours reinforces the idea that urine contains a variety of viable cell types with relevant applications. In this review, we describe those cell types and their potential uses...
  42. doi request reprint Design, synthesis, and biological evaluation of novel conformationally constrained inhibitors targeting epidermal growth factor receptor threonine⁷⁹⁰ → methionine⁷⁹⁰ mutant
    Shaohua Chang
    Key Laboratory of Regenerative Biology and Institute of Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Guangzhou 510530, China
    J Med Chem 55:2711-23. 2012
    ..Moreover, 2s displayed promising anticancer efficacy in a human NSCLC (H1975) xenograft nude mouse model...
  43. pmc A SNX10/V-ATPase pathway regulates ciliogenesis in vitro and in vivo
    Yanqun Chen
    CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China
    Cell Res 22:333-45. 2012
    ....
  44. doi request reprint Rescue of ATP7B function in hepatocyte-like cells from Wilson's disease induced pluripotent stem cells using gene therapy or the chaperone drug curcumin
    Shiqiang Zhang
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, People s Republic of China
    Hum Mol Genet 20:3176-87. 2011
    ....
  45. pmc MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition
    Baojian Liao
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China
    J Biol Chem 286:17359-64. 2011
    ..Our work thus provides an interesting alternative for improving reprogramming using miRNAs and adds new evidence for the emerging relationship between pluripotency and the epithelial phenotype...
  46. doi request reprint Porcine induced pluripotent stem cells may bridge the gap between mouse and human iPS
    Miguel A Esteban
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    IUBMB Life 62:277-82. 2010
    ..However, although these studies are relevant and have created significant interest they face analogous problems that we discuss herein together with potential new directions...
  47. ncbi request reprint Sorting nexin 10 induces giant vacuoles in mammalian cells
    Baoming Qin
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 281:36891-6. 2006
    ..Brefeldin A, a chemical known to block the endoplasmic reticulum to Golgi transport, inhibited the vacuolization process. Together, these results suggest that SNX10 activity may be involved in the regulation of endosome homeostasis...
  48. pmc Stem-like cancer cells are inducible by increasing genomic instability in cancer cells
    Yi Liang
    State Key Laboratory of Oncology in Southern China, Guangzhou, China
    J Biol Chem 285:4931-40. 2010
    ..Our study thus suggested that, beside transformation of tissue stem cells leading to CSC generation, genomic instability could be another potential mechanism resulting in SLCC formation, especially at tumor recurrence stage...
  49. ncbi request reprint Rapid inactivation of alpha-1-proteinase inhibitor by neutrophil specific leukolysin/membrane-type matrix metalloproteinase 6
    Jing Nie
    Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Cell Res 296:145-50. 2004
    ..Taken together, these results suggest that neutrophils may mediate tissue destruction by deploying leukolysin to weaken the alpha1-PI protective shield at inflammatory sites...
  50. doi request reprint Vitamin C modulates TET1 function during somatic cell reprogramming
    Jiekai Chen
    1 Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 2 Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 3
    Nat Genet 45:1504-9. 2013
    ..Given its benefit to human health, vitamin C should be investigated further for its role in epigenetic regulation. ..
  51. pmc Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays
    Yang Deng
    Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 130:6267-71. 2008
    ..These demonstrations establish the viability of the fluidic and air-stable SLB platform for generating content microarrays in high throughput studies, e.g., the screening of drugs and nanomedicine targeting cell surface receptors...
  52. pmc VHL inactivation induces HEF1 and Aurora kinase A
    Jianyong Xu
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    J Am Soc Nephrol 21:2041-6. 2010
    ..Suppression of this pathway improved the formation of primary cilia and reduced cell motility in VHL-defective renal cancer cells. Our results highlight the gatekeeper role of VHL in the kidney epithelium...
  53. ncbi request reprint Stem cell pluripotency and transcription factor Oct4
    Guang Jin Pan
    Department of Pharmacology, School of Sciences, Tsinghua University, Beijing 100084, China
    Cell Res 12:321-9. 2002
    ..In this review, we summarize the structure and function of Oct4 and address issues related to Oct4 function in maintaining totipotency or pluripotency of embryonic stem cells...
  54. pmc Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells
    Jiekai Chen
    Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China
    J Biol Chem 285:31066-72. 2010
    ..Moreover, we could reprogram meningeal cells using only two factors Oct4/Klf4. Therefore, iSF1 represents a basal medium that may be used for mechanistic studies and testing new reprogramming approaches...
  55. ncbi request reprint Immobilization of oriented protein molecules on poly(ethylene glycol)-coated Si(111)
    Taewoon Cha
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Proteomics 4:1965-76. 2004
    ..This kind of quantitative analysis is essential in tuning surface concentration and chemical environment for optimal sensitivity in probe-target interaction...
  56. doi request reprint Synthesis of aza-fused polycyclic quinolines through copper-catalyzed cascade reactions
    Qian Cai
    Key Laboratory of Regenerative Biology and Institute of Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, no 190, Kaiyuan Avenue, Science Park, Guangzhou, China, 510530
    Org Lett 12:1500-3. 2010
    ..The method is applied to a wide range of 2-iodo, 2-bromo, and 2-chloro aryl aldehyde substrates to yield the aza-fused polycyclic quinolines in good yields...
  57. doi request reprint Vitamin C improves the quality of somatic cell reprogramming
    Miguel A Esteban
    Stem Cell and Cancer Biology Group at the Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Nat Genet 44:366-7. 2012
    ..A new study now shows that vitamin C enhances the quality of somatic cell reprogramming in mice, highlighting the possibility that further manipulation of culture conditions could improve this technology for regenerative medicine...
  58. pmc Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells
    Quan Wang
    State Key Laboratory of Drug Research, The National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
    Cell Res 21:1424-35. 2011
    ..Our results not only provide a straightforward method to improve the iPSC generation efficiency, but also identified a histone demethylase as a critical modulator for somatic cell reprogramming...
  59. doi request reprint Atovaquone derivatives as potent cytotoxic and apoptosis inducing agents
    Jing Zhou
    Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, International Business Incubator A 3, Guangzhou Science Park, Guangzhou, China
    Bioorg Med Chem Lett 19:5091-4. 2009
    ..Several compounds displayed significantly improved cytotoxic activities against a panel of cancer cell lines than that of atovaquone. These compounds also induced apoptosis through activating pro-apoptotic caspases 9 and 3...
  60. ncbi request reprint Proprotein convertase furin interacts with and cleaves pro-ADAMTS4 (Aggrecanase-1) in the trans-Golgi network
    Ping Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:15434-40. 2004
    ..These results indicate that the activation mechanism for ADAMTS4 can be targeted for therapeutical intervention against this enzyme...
  61. pmc Reprogramming of mouse and human somatic cells by high-performance engineered factors
    Yang Wang
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    EMBO Rep 12:373-8. 2011
    ..Synthetic factor-based reprogramming might lead to a paradigm shift in reprogramming research...
  62. pmc Mouse meningiocytes express Sox2 and yield high efficiency of chimeras after nuclear reprogramming with exogenous factors
    Dajiang Qin
    Stem Cell and Cancer Biology Group, Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663
    J Biol Chem 283:33730-5. 2008
    ..Our results define a population of somatic cells that are ready to undergo iPS, thus highlighting a very attractive cell type for iPS research and application...
  63. doi request reprint Zfyve9a regulates the proliferation of hepatic cells during zebrafish embryogenesis
    Nian Liu
    Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, China
    Int J Dev Biol 57:773-8. 2013
    ..Thus, our results reveal the liver-specific function of zfyve9a during early embryogenesis and indicate that the zfyve9a mediated signal is essential for the proliferation of hepatic cells during the expansion of liver bud. ..
  64. pmc Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells
    Yanting Xue
    CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China
    PLoS ONE 8:e70573. 2013
    ..The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells. ..
  65. pmc Low immunogenicity of neural progenitor cells differentiated from induced pluripotent stem cells derived from less immunogenic somatic cells
    Pengfei Liu
    Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong Province, PR China
    PLoS ONE 8:e69617. 2013
    ....
  66. doi request reprint Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells
    Guangjin Pan
    CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
    Bioessays 34:472-6. 2012
    ..In this essay, we discuss this progress and the potentials of these two reprogramming approaches for regenerative medicine...
  67. ncbi request reprint Total synthesis of cyclic tetrapeptide FR235222, a potent immunosuppressant that inhibits mammalian histone deacetylases
    Weiqing Xie
    State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Lu, Shanghai 200032, China
    Org Lett 7:2775-7. 2005
    ....
  68. ncbi request reprint Order from chaos: single cell reprogramming in two phases
    Guangjin Pan
    CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Stem Cell 11:445-7. 2012
    ..Recently in Cell, Buganim et al. (2012) changed this view using single cell analyses to reveal a stochastic early and hierarchical late phase, with implications for productive alternative reprogramming strategies...
  69. ncbi request reprint Direct activation of pro-matrix metalloproteinase-2 by leukolysin/membrane-type 6 matrix metalloproteinase/matrix metalloproteinase 25 at the asn(109)-Tyr bond
    Jing Nie
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Cancer Res 63:6758-62. 2003
    ..Taken these facts together, we conclude that MT6-MMP may participate in tumor invasion and metastasis by directly converting proMMP-2 into active form...
  70. pmc Generation of induced pluripotent stem cells from urine
    Ting Zhou
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China
    J Am Soc Nephrol 22:1221-8. 2011
    ..This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs...
  71. ncbi request reprint Distinct roles of catalytic and pexin-like domains in membrane-type matrix metalloproteinase (MMP)-mediated pro-MMP-2 activation and collagenolysis
    Aixiang Jiang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 278:38765-71. 2003
    ..Natl. Acad. Sci. U. S. A. 98, 13693-13698). Taken together, these results demonstrate that domains in MT-MMPs function differently toward a given substrate and thus should be targeted differentially for future therapeutic development...
  72. ncbi request reprint Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4
    Dajiang Qin
    Cell Res 17:959-62. 2007
  73. ncbi request reprint Aromatic residues in the C-terminal domain 2 are required for Nanog to mediate LIF-independent self-renewal of mouse embryonic stem cells
    Zhe Wang
    Laboratory of Stem Cell Biology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084 corrected China
    J Biol Chem 283:4480-9. 2008
    ..Taken together, our results demonstrate that the CD2 domain of Nanog is a unique transactivator that utilizes aromatic residues to confer specific activity absolutely required for ES self-renewal...
  74. ncbi request reprint The stem cell pluripotency factor NANOG activates transcription with two unusually potent subdomains at its C terminus
    Guangjin Pan
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, Institutes of Biomedicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, 100084 Beijing, China
    J Biol Chem 280:1401-7. 2005
    ..These data suggest that either WR or CD2 is sufficient for NANOG to function as a transactivator...
  75. ncbi request reprint Intracellular activation of human adamalysin 19/disintegrin and metalloproteinase 19 by furin occurs via one of the two consecutive recognition sites
    Tiebang Kang
    Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee 32306 4390, USA
    J Biol Chem 277:25583-91. 2002
    ..This report is the first thorough investigation of the intracellular activation of adamalysin 19, demonstrating that furin activated pro-hADAM19 in the secretory pathway via one of the two consecutive furin recognition sites...
  76. ncbi request reprint Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals
    Ping Cui
    Institute of Pharmacology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua Institutes of Biomedical Sciences, Tsinghua University, Beijing, China
    Exp Cell Res 293:154-63. 2004
    ..Finally, multiple nuclear localization signals were identified in PSR sequence, each capable of targeting GFP to the nuclei. Together, these results suggest that PSR may serve a dual role both on the cell surface and in the nuclei...
  77. ncbi request reprint Membrane type-matrix metalloproteinases (MT-MMP)
    Stanley Zucker
    VA Medical Center, Northport, New York 11768, USA
    Curr Top Dev Biol 54:1-74. 2003
  78. ncbi request reprint The roles of substrate thermal stability and P2 and P1' subsite identity on matrix metalloproteinase triple-helical peptidase activity and collagen specificity
    Dmitriy Minond
    Department of Chemistry and Biochemistry, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431 0991, USA
    J Biol Chem 281:38302-13. 2006
    ..Further exploration of MMP active sites and exosites, in combination with substrate conformation, may prove valuable for additional dissection of collagenolysis and yield information useful in the design of more selective MMP inhibitors...
  79. ncbi request reprint A dominant-negative form of mouse SOX2 induces trophectoderm differentiation and progressive polyploidy in mouse embryonic stem cells
    Jun Li
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 282:19481-92. 2007
    ..These results suggest that SOX2 maintains stem cell pluripotency by shuttling between the nucleus and cytoplasm in cooperation with OCT4 to prevent trophectoderm differentiation and polyploid formation in ES cells...
  80. ncbi request reprint Genomic instability in laminopathy-based premature aging
    Baohua Liu
    Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Hong Kong
    Nat Med 11:780-5. 2005
    ..Our results indicate that unprocessed prelamin A and truncated lamin A act dominant negatively to perturb DNA damage response and repair, resulting in genomic instability which might contribute to laminopathy-based premature aging...