Affiliation: Central South University
Ma C, Liu H, Li W, Chen H, Jin S, Wang J, et al
. Label-free monitoring of DNA methyltransferase activity based on terminal deoxynucleotidyl transferase using a thioflavin T probe. Mol Cell Probes. 2016;30:118-21 pubmed publisher
..And because no labeling with a fluorophore-quencher pair was required, it is simple and low cost. We envision that our novel fluorescent detection method for Dam MTase activity could be applied as a useful tool in biomedical research. ..
Li Z, Guo X, Tang L, Peng L, Chen M, Luo X, et al
. Methylation analysis of plasma cell-free DNA for breast cancer early detection using bisulfite next-generation sequencing. Tumour Biol. 2016;37:13111-13119 pubmed
..Quantitative analysis of methylation pattern of plasma cfDNA by next-generation sequencing might be a valuable non-invasive tool for early detection of breast cancer. ..
Ma C, Jin S, Liu H, Xia K, Tang J, Wang K, et al
. Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors. Mol Cell Probes. 2015;29:500-502 pubmed publisher
..01 unit/mL limit of detection, which is better than those with labeled fluorophores. Furthermore, T4 PNKP inhibition by the inhibitor heparin is shown, demonstrating the potential to screen suitable inhibitor drugs for T4 PNKP. ..
Ma C, Wu K, Liu H, Xia K, Wang K, Wang J. Label-free fluorescence turn-on detection of uracil DNA glycosylase activity based on G-quadruplex formation. Talanta. 2016;160:449-453 pubmed publisher
..This new method could be used to evaluate the inhibition effect of 5-fluorouracil on UDG activity, and become a useful tool in biomedical research. ..
Li Z, Heng J, Yan J, Guo X, Tang L, Chen M, et al
. Integrated analysis of gene expression and methylation profiles of 48 candidate genes in breast cancer patients. Breast Cancer Res Treat. 2016;160:371-383 pubmed
..These findings would contribute to molecular characterization and identification of biomarkers for potential clinical applications. ..
Ma C, Liu H, Wu K, Chen M, Zheng L, Wang J. An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA. Sensors (Basel). 2017;17: pubmed publisher
..5 nM-10 times lower than a previously reported quencher-free fluorescence method. Furthermore, this method has potential applications in clinical diagnosis and biomedical research. ..
Li G, Guo X, Tang L, Chen M, Luo X, Peng L, et al
. Analysis of BRCA1/2 mutation spectrum and prevalence in unselected Chinese breast cancer patients by next-generation sequencing. J Cancer Res Clin Oncol. 2017;143:2011-2024 pubmed publisher
..Our study provided a reliable and effective platform for BRCA1/2 genetic testing, and suggested that there was a relatively high prevalence and special spectrum of BRCA1/2 mutations in unselected Chinese breast cancer patients. ..