Research Topics
| Dan DuSummaryAffiliation: Central China Normal University Country: China Publications
| Collaborators
|
Detail Information
Publications
Integrated lateral flow test strip with electrochemical sensor for quantification of phosphorylated cholinesterase: biomarker of exposure to organophosphorus agentsDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Anal Chem 84:1380-5. 2012..It is baseline free and thus provides a rapid, sensitive, selective, and inexpensive tool for in-field and point-of-care assessment of exposures to OP pesticides and nerve agents...- Development of acetylcholinesterase biosensor based on CdTe quantum dots/gold nanoparticles modified chitosan microspheres interfaceDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China
Biosens Bioelectron 24:475-9. 2008..3 ng mL(-1). The proposed biosensor showed good precision and reproducibility, acceptable stability and accuracy in garlic samples analysis... - Electrochemical pesticide sensitivity test using acetylcholinesterase biosensor based on colloidal gold nanoparticle modified sol-gel interfaceDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Talanta 74:766-72. 2008..This method could be developed as a conventional method to select efficient enzyme inhibitors and investigate toxic compounds against to enzyme... - Immobilization of acetylcholinesterase based on the controllable adsorption of carbon nanotubes onto an alkanethiol monolayer for carbaryl sensingDan Du
Department of Chemistry, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan, China
Analyst 133:1790-5. 2008..001 to 1 microg mL(-1) and 2 to 15 microg mL(-1), with a detection limit of 0.6 ng mL(-1). The determination of carbaryl in garlic samples showed acceptable accuracy, which provided a new promising tool for analysis of enzyme inhibitors... 
Comparison of pesticide sensitivity by electrochemical test based on acetylcholinesterase biosensorDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Biosens Bioelectron 23:285-9. 2007....- Multiplexed electrochemical immunoassay of phosphorylated proteins based on enzyme-functionalized gold nanorod labels and electric field-driven accelerationDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Anal Chem 83:6580-5. 2011..The disposable immunosensor array shows excellent promise for clinical screening of phosphorylated proteins and convenient point-of-care diagnostics... - Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol-gel film for amperometric detection of organophosphorous insecticideDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Biosens Bioelectron 23:130-4. 2007..9930 and 0.9985, respectively. The detection limit was 0.6 ng/ml at a 10% inhibition. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors... - Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: an exposure biomarker of organophosphatesDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, Hubei Province, PR China
Biosens Bioelectron 26:4231-5. 2011..05 to 20 nM, with detection limit of 0.02 nM. This new oxidative desorption assay thus provides a sensitive and quantitative tool for biomonitoring of the exposure to OP pesticides and nerve agents... - Covalent coupling of organophosphorus hydrolase loaded quantum dots to carbon nanotube/Au nanocomposite for enhanced detection of methyl parathionDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, Hubei Province, PR China
Biosens Bioelectron 25:1370-5. 2010..Unlike cholinesterase-based biosensor, the MPDE biosensor can be potentially reused and is suitable for continuous monitoring... - Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphatesDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Biosens Bioelectron 25:2503-8. 2010..01 to 0.5 microg/mL and from 1 to 25 microg/mL, with a detection limit of 1.0 ng/mL. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors... - One-step electrochemically deposited gold nanoparticles interface grafted with avidin for acetylcholinesterase biosensor designWeiying Zhang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
J Nanosci Nanotechnol 10:5685-91. 2010..05 to 15 microg mL(-1) with detection limit of 0.001 microg mL(-1). The simple method showed good fabrication reproducibility and acceptable stability, which provided a new avenue for electrochemical biosensor design... - Highly sensitive and selective immuno-capture/electrochemical assay of acetylcholinesterase activity in red blood cells: a biomarker of exposure to organophosphorus pesticides and nerve agentsAiqiong Chen
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Environ Sci Technol 46:1828-33. 2012..It offers a new approach for sensitive, selective, and rapid AChE activity assay for biomonitoring of exposure to OPs... - Acetylcholinesterase biosensor based on gold nanoparticles and cysteamine self assembled monolayer for determination of monocrotophosDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P R China
J Nanosci Nanotechnol 9:2368-73. 2009.... - Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticidesLin Zhang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Nanoscale 4:4674-9. 2012..1 ng mL(-1). These results suggest that the PBNCs/rGO hybrids nanocomposite exhibited high electrocatalytic activity towards the oxidation of thiocholine, which lead to the sensitive detection of OP pesticides... - An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube-cross-linked chitosan compositeDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan, 430079, China
Anal Bioanal Chem 387:1059-65. 2007..10 micromol L(-1). Fabrication reproducibility of the sensor was good and the stability was acceptable. The sensor is a promising new tool for characterization of enzyme inhibitors and for pesticide analysis. Abstract... - CdTe nanocrystal-based electrochemical biosensor for the recognition of neutravidin by anodic stripping voltammetry at electrodeposited bismuth filmDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Biosens Bioelectron 24:869-74. 2008..Unlike earlier two-step sandwich bioassays, the present protocol relies on a one-step competitive assay, which is more accurate and sensitive, showing great promise for rapid, simple and cost-effective analysis of protein... - Methyl parathion hydrolase based nanocomposite biosensors for highly sensitive and selective determination of methyl parathionShizhen Chen
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str 152, Wuhan 430079, Hubei Province, PR China
Biosens Bioelectron 26:4320-5. 2011..The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species... - In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor designDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Talanta 74:1337-43. 2008..001 to 0.1 microg mL(-1) and from 0.1 to 25 microg mL(-1), with detection limit of 0.001 microg mL(-1). The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design... - Determination of carbaryl pesticide using amperometric acetylcholinesterase sensor formed by electrochemically deposited chitosanDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Colloids Surf B Biointerfaces 58:145-50. 2007..The developed sensor exhibited good fabrication reproducibility and acceptable stability, which provided a new promising tool for pesticide analysis... - A gold nanoparticle labeling strategy for the sensitive kinetic assay of the carbamate-acetylcholinesterase interaction by surface plasmon resonanceXi Huang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China
Talanta 78:1036-42. 2009..This AuNPs labeling strategy is versatile and may be applicable for the direct or competitive SPR kinetic assay of the interaction between small molecule inhibitors and their target proteins with a high sensitivity... - Detection of organophosphate pesticide using polyaniline and carbon nanotubes composite based on acetylcholinesterase inhibitionDaiqin Chen
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
J Nanosci Nanotechnol 10:5662-6. 2010..The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity... - Graphene-based immunosensor for electrochemical quantification of phosphorylated p53 (S15)Yunying Xie
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, PR China
Anal Chim Acta 699:44-8. 2011..This easily fabricated immunosensor provides a new promising tool for analysis of phospho-p53(15) and other phosphorylated proteins... - Rational design and application of molecularly imprinted sol-gel polymer for the electrochemically selective and sensitive determination of Sudan IShizhen Chen
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Talanta 84:451-6. 2011..0 × 10(-5)M, with a detection limit of 2.0 × 10(-9)M. The strategy is generally applicable in developing sensitive, selective, and moreover, reusable electrochemical sensors for quantitative determination of electroactive species... - Functionalized graphene oxide as a nanocarrier in a multienzyme labeling amplification strategy for ultrasensitive electrochemical immunoassay of phosphorylated p53 (S392)Dan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, People s Republic of China
Anal Chem 83:746-52. 2011..8%). This simple and low-cost immunosensor shows great promise for detection of other phosphorylated proteins and clinical applications... - Electrochemical immunoassay of membrane P-glycoprotein by immobilization of cells on gold nanoparticles modified on a methoxysilyl-terminated butyrylchitosan matrixDan Du
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, PR China
Biochemistry 44:11539-45. 2005..0 x 10(4) cells mL(-)(1). The results were comparable to flow cytometric analysis of P-gp expression. This proposed method was practical, convenient, and significant in the clinic and cytobiology... 
Biomonitoring of organophosphorus agent exposure by reactivation of cholinesterase enzyme based on carbon nanotube-enhanced flow-injection amperometric detectionDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Anal Chem 81:9314-20. 2009....- Rhodamine-based ratiometric fluorescence sensing for the detection of mercury(II) in aqueous solutionHaizhu Liu
Key Laboratory of Analysis and Detection Technology for Food Safety, Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
Talanta 81:433-7. 2010..The proposed approach was successfully applied to the determination of Hg(2+) in water samples... - One-step electrodeposition of a molecularly imprinting chitosan/phenyltrimethoxysilane/AuNPs hybrid film and its application in the selective determination of p-nitrophenolShanshan Li
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P R China
Analyst 138:2761-8. 2013..The methodology is extendable as a simple and general platform for developing hybrid film sensors for the specific determination of various electrochemically active species... - Immunological assay for carbohydrate antigen 19-9 using an electrochemical immunosensor and antigen immobilization in titania sol-gel matrixDan Du
State Key Laboratory of Coordination Chemistry, Department of Chemistry, Institute of Analytical Science, Nanjing University, 210093 Nanjing, PR China
J Immunol Methods 283:67-75. 2003..The proposed immunosensor provides a new promising tool for the clinical immunoassay of CA19-9... - The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathionYuting Zhao
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Nanoscale 5:1121-6. 2013..The simplicity and ease of operation of the proposed method has great potential for on-site detection of P-S containing pesticides and provides a promising strategy to construct a robust biosensor... - Preparation, characterization of Fe3O4 at TiO2 magnetic nanoparticles and their application for immunoassay of biomarker of exposure to organophosphorus pesticidesXiao Zhang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
Biosens Bioelectron 41:669-74. 2013..It offers a new method for rapid, sensitive, selective and inexpensive screening/evaluating exposure to OP pesticides and nerve agents... - Determination of trace aluminum in biological and water samples by cloud point extraction preconcentration and graphite furnace atomic absorption spectrometry detectionHongbo Sang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
J Hazard Mater 154:1127-32. 2008..09 ng mL(-1), and the relative standard deviation is 4.7% at 10 ng mL(-1) Al(III) level (n=7). The proposed method has been applied for determination of trace amount of aluminum in biological and water samples with satisfactory results... - Reagentless amperometric carbohydrate antigen 19-9 immunosensor based on direct electrochemistry of immobilized horseradish peroxidaseDan Du
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
Talanta 71:1257-62. 2007..0 PBS at 4 degrees C for 10 days. This method avoids the addition of electron transfer mediator, thus simplifies the immunoassay procedure and decreases the analytical time. It provides a new promising platform for clinical immunoassay... - Colloidal gold nanoparticle modified carbon paste interface for studies of tumor cell adhesion and viabilityDan Du
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China, Nanjing University, Nanjing 210093, PR China
Biomaterials 26:6487-95. 2005..This simple and rapid method could be applied for the electrochemical investigation of exogenous effect and characterization of the viability of living cells... - Recognition of glycoprotein peroxidase via Con A-carrying self-assembly layer on goldSongqin Liu
College of Chemistry and Chemical Engineering, Southeast University, Nanjing, PR China
Biomacromolecules 8:2142-8. 2007..The specific binding achieved is desirable in biosensor fabrication, glycoprotein separation, recognition, and purification as well as in drug-releasing systems... - Construction of a biomimetic zwitterionic interface for monitoring cell proliferation and apoptosisDan Du
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China, Department of Chemistry, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing, People's Republic of China
Langmuir 21:8394-9. 2005..Thus, this man-made interface would be applicable to the growth of cells and the study of biomaterial-cell interaction and has potential applications in medicine and cytobiology... - Electrochemical antitumor drug sensitivity test for leukemia K562 cells at a carbon-nanotube-modified electrodeJing Chen
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China
Chemistry 11:1467-72. 2005..The method proposed could be developed as a convenient means to study the sensitivity of tumor cells to antitumor drugs...