Ricardo Cabrera

Summary

Affiliation: Universidad de Chile
Country: Chile

Publications

  1. pmc The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: kinetic and structural analysis of the allosteric ATP inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    J Biol Chem 286:5774-83. 2011
  2. doi request reprint Crystallographic structure of phosphofructokinase-2 from Escherichia coli in complex with two ATP molecules. Implications for substrate inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    J Mol Biol 383:588-602. 2008
  3. ncbi request reprint Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli
    Andrés Caniuguir
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    FEBS Lett 579:2313-8. 2005
  4. ncbi request reprint Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli
    Mauricio Baez
    Departamento de Biologia, Facultad de Ciencias Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 46:6141-8. 2007
  5. pmc Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase family
    Ricardo Cabrera
    Laboratorio de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:935-7. 2006
  6. ncbi request reprint Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family member
    Rafael E Parducci
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 45:9291-9. 2006
  7. doi request reprint Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coli
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 502:23-30. 2010
  8. ncbi request reprint Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle x-ray scattering and homology modeling
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    J Biol Chem 278:12913-9. 2003
  9. pmc A ribokinase family conserved monovalent cation binding site enhances the MgATP-induced inhibition in E. coli phosphofructokinase-2
    Mauricio Baez
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, Santiago, Chile
    Biophys J 105:185-93. 2013
  10. ncbi request reprint Ligand-dependent structural changes and limited proteolysis of Escherichia coli phosphofructokinase-2
    Ricardo Cabrera
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 406:289-95. 2002

Collaborators

  • Victoria Guixé
  • Claudia Stange
  • Stefano Trapani
  • Mauricio Baez
  • Jorge Babul
  • Andrés Caniuguir
  • María Francisca Aguayo
  • Karel Olavarria
  • Jennifer Alcaíno
  • Rafael E Parducci
  • Humberto M Pereira
  • Teresa Altabella
  • Rodrigo Munoz
  • Richard C Garratt
  • Alejandro Blanco
  • Roberto Parada
  • Diego Ampuero
  • Michael Handford
  • Marta Gallart
  • Pablo Villalobos
  • Patricio Mandujano
  • César A Ramírez-Sarmiento
  • Víctor Cifuentes
  • Marcelo Baeza
  • Matías Fuentealba
  • Diego Valdés
  • Claudio C Vásquez

Detail Information

Publications13

  1. pmc The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: kinetic and structural analysis of the allosteric ATP inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    J Biol Chem 286:5774-83. 2011
    ..Differences and resemblances in the patterns of fructose-6-P binding and the mechanism of inhibition are discussed for Pfk-1 and Pfk-2, as an example of evolutionary convergence, because these enzymes do not share a common ancestor...
  2. doi request reprint Crystallographic structure of phosphofructokinase-2 from Escherichia coli in complex with two ATP molecules. Implications for substrate inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    J Mol Biol 383:588-602. 2008
    ..This structure also provides the grounds to compare analogous features of the nonhomologous phosphofructokinases from E. coli...
  3. ncbi request reprint Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli
    Andrés Caniuguir
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    FEBS Lett 579:2313-8. 2005
    ....
  4. ncbi request reprint Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli
    Mauricio Baez
    Departamento de Biologia, Facultad de Ciencias Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 46:6141-8. 2007
    ....
  5. pmc Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase family
    Ricardo Cabrera
    Laboratorio de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:935-7. 2006
    ..8, b = 86.8, c = 171.3 A. The calculated Matthews coefficient of 2.45 A(3) Da(-1) indicates the presence of two monomers in the asymmetric unit, corresponding to a solvent content of 49%. Structure determination is ongoing...
  6. ncbi request reprint Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family member
    Rafael E Parducci
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 45:9291-9. 2006
    ..Given that the E190Q mutant presents alterations in the inhibition by MgATP(2-) and phosphate, we conclude that the E190 residue has a role not only in catalysis but also in allosteric regulation...
  7. doi request reprint Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coli
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 502:23-30. 2010
    ..Site-directed mutagenesis of R90 and D256 present in these motifs, indicate that R90 participates in the binding of the phosphorylated substrate and that D256 is involved in the phosphoryl transfer mechanism...
  8. ncbi request reprint Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle x-ray scattering and homology modeling
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    J Biol Chem 278:12913-9. 2003
    ..Based on these findings, a proposal is made to account for the inhibitory effect of MgATP on the enzymatic activity...
  9. pmc A ribokinase family conserved monovalent cation binding site enhances the MgATP-induced inhibition in E. coli phosphofructokinase-2
    Mauricio Baez
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, Santiago, Chile
    Biophys J 105:185-93. 2013
    ..These results indicate that binding of the monovalent metal ions indirectly influences the allosteric site of Pfk-2 by increasing its affinity for MgATP with no alteration in the conformation of residues present at the catalytic site...
  10. ncbi request reprint Ligand-dependent structural changes and limited proteolysis of Escherichia coli phosphofructokinase-2
    Ricardo Cabrera
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 406:289-95. 2002
    ....
  11. doi request reprint Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana
    María Francisca Aguayo
    Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile
    Plant Sci 205:63-75. 2013
    ..This evidence suggests that limitations in the metabolism of sugar alcohols alter the growth of Arabidopsis and its response to drought...
  12. doi request reprint The cofactor preference of glucose-6-phosphate dehydrogenase from Escherichia coli--modeling the physiological production of reduced cofactors
    Karel Olavarria
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    FEBS J 279:2296-309. 2012
    ..Nevertheless, we show that certain combinations of rR(NADP) and rR(NAD) sustain greater levels of NADH production over NADPH...
  13. ncbi request reprint Modeling the interfacial interactions between CrtS and CrtR from Xanthophyllomyces dendrorhous , a P450 system involved in astaxanthin production
    Jennifer Alcaíno
    Centro de Biotecnología y Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile
    J Agric Food Chem 60:8640-7. 2012
    ..Compared with the possible interface between CrtS and both FMNbd, the Xanthophyllomyces system appears to be stabilized by more saline bridges...