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Genomes and Genes | Ricardo CabreraSummaryAffiliation: Universidad de Chile Country: Chile Publications
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Publications
The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: kinetic and structural analysis of the allosteric ATP inhibitionRicardo Cabrera
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
J Biol Chem 286:5774-83. 2011..Differences and resemblances in the patterns of fructose-6-P binding and the mechanism of inhibition are discussed for Pfk-1 and Pfk-2, as an example of evolutionary convergence, because these enzymes do not share a common ancestor...
Crystallographic structure of phosphofructokinase-2 from Escherichia coli in complex with two ATP molecules. Implications for substrate inhibitionRicardo Cabrera
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
J Mol Biol 383:588-602. 2008..This structure also provides the grounds to compare analogous features of the nonhomologous phosphofructokinases from E. coli...
Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coliMauricio Baez
Departamento de Biologia, Facultad de Ciencias Universidad de Chile, Casilla 653, Santiago, Chile
Biochemistry 46:6141-8. 2007....- Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coliAndrés Caniuguir
Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
FEBS Lett 579:2313-8. 2005.... - Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase familyRicardo Cabrera
Laboratorio de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:935-7. 2006..8, b = 86.8, c = 171.3 A. The calculated Matthews coefficient of 2.45 A(3) Da(-1) indicates the presence of two monomers in the asymmetric unit, corresponding to a solvent content of 49%. Structure determination is ongoing... - Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family memberRafael E Parducci
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Biochemistry 45:9291-9. 2006..Given that the E190Q mutant presents alterations in the inhibition by MgATP(2-) and phosphate, we conclude that the E190 residue has a role not only in catalysis but also in allosteric regulation... - Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coliRicardo Cabrera
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Arch Biochem Biophys 502:23-30. 2010..Site-directed mutagenesis of R90 and D256 present in these motifs, indicate that R90 participates in the binding of the phosphorylated substrate and that D256 is involved in the phosphoryl transfer mechanism... - Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle x-ray scattering and homology modelingRicardo Cabrera
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
J Biol Chem 278:12913-9. 2003..Based on these findings, a proposal is made to account for the inhibitory effect of MgATP on the enzymatic activity... - Ligand-dependent structural changes and limited proteolysis of Escherichia coli phosphofructokinase-2Ricardo Cabrera
Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Arch Biochem Biophys 406:289-95. 2002.... - The cofactor preference of glucose-6-phosphate dehydrogenase from Escherichia coli--modeling the physiological production of reduced cofactorsKarel Olavarria
Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
FEBS J 279:2296-309. 2012..Nevertheless, we show that certain combinations of rR(NADP) and rR(NAD) sustain greater levels of NADH production over NADPH... - Modeling the interfacial interactions between CrtS and CrtR from Xanthophyllomyces dendrorhous , a P450 system involved in astaxanthin productionJennifer Alcaíno
Centro de Biotecnología y Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile
J Agric Food Chem 60:8640-7. 2012..Compared with the possible interface between CrtS and both FMNbd, the Xanthophyllomyces system appears to be stabilized by more saline bridges...