Affiliation: Universidad de Chile
AlcaÃno J, Cifuentes V, Baeza M. Physiological adaptations of yeasts living in cold environments and their potential applications. World J Microbiol Biotechnol. 2015;31:1467-73 pubmed publisher
..In this minireview, we describe the physiological adaptations of several psychrophilic yeasts and their possible biotechnological applications. ..
Carrasco M, Villarreal P, Barahona S, AlcaÃno J, Cifuentes V, Baeza M. Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts. BMC Microbiol. 2016;16:21 pubmed publisher
..However, Rh. glacialis and M. blollopis displayed high amylase or cellulase activity, respectively, under 22Â Â°C. In this sense, these yeasts are interesting candidates for industrial processes that require lower temperatures. ..
Alcaíno J, Baeza M, Cifuentes V. Carotenoid Distribution in Nature. Subcell Biochem. 2016;79:3-33 pubmed publisher
..Finally, as an example, the synthesis of the xanthophyll astaxanthin is discussed. ..
Martinez Moya P, Niehaus K, AlcaÃno J, Baeza M, Cifuentes V. Proteomic and metabolomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous using different carbon sources. BMC Genomics. 2015;16:289 pubmed publisher
..The high metabolite level generated in this pathway could increase the cellular respiration rate, producing reactive oxygen species, which induces carotenogenesis. ..
Leiva K, Werner N, SepÃºlveda D, Barahona S, Baeza M, Cifuentes V, et al
. Identification and functional characterization of the CYP51 gene from the yeast Xanthophyllomyces dendrorhous that is involved in ergosterol biosynthesis. BMC Microbiol. 2015;15:89 pubmed publisher
..These results suggest that the CYP51 gene identified in X. dendrorhous encodes a functional sterol C14-demethylase that is involved in ergosterol biosynthesis. ..
Werner N, Gomez M, Baeza M, Cifuentes V, Alcaíno J. Functional characterization of thiolase-encoding genes from Xanthophyllomyces dendrorhous and their effects on carotenoid synthesis. BMC Microbiol. 2016;16:278 pubmed
..The POT1 gene would encode a functional 3-ketoacyl Co-A thiolase that is non-essential for cell growth, but its mutation indirectly affects pigment production. ..
Baeza M, AlcaÃno J, Barahona S, SepÃºlveda D, Cifuentes V. Codon usage and codon context bias in Xanthophyllomyces dendrorhous. BMC Genomics. 2015;16:293 pubmed publisher
..dendrorhous, which is more evident for ORFs that are expressed at the highest levels under the studied conditions. However, there is no direct relation between the totiviral ORF biases and the host ORFs. ..
CÃ³rdova P, Gonzalez A, Nelson D, GutiÃ©rrez M, Baeza M, Cifuentes V, et al
. Characterization of the cytochrome P450 monooxygenase genes (P450ome) from the carotenogenic yeast Xanthophyllomyces dendrorhous. BMC Genomics. 2017;18:540 pubmed publisher
..These findings established a basis for future studies about the role of P450s in the carotenogenic yeast X. dendrorhous and their potential biotechnological applications. ..
Contreras G, Barahona S, Rojas M, Baeza M, Cifuentes V, Alcaíno J. Increase in the astaxanthin synthase gene (crtS) dose by in vivo DNA fragment assembly in Xanthophyllomyces dendrorhous. BMC Biotechnol. 2013;13:84 pubmed publisher
..This methodology could be used to easily and rapidly overexpress individual genes or combinations of genes simultaneously in X. dendrorhous, eliminating numerous steps involved in conventional cloning methods. ..