Cristian Gallardo Escárate


Country: Chile


  1. Núñez Acuña G, Gallardo Escárate C, Skiftesvik A, Fields D, Browman H. Silencing of ionotropic receptor 25a decreases chemosensory activity in the salmon louse Lepeophtheirus salmonis during the infective stage. Gene. 2019;697:35-39 pubmed publisher
    ..salmonis chemosensory perception system was not fully activated due to gene silencing. The results of this study demonstrate the role of ionotropic receptor 25a during host recognition by sea lice. ..
  2. Núñez Acuña G, Gallardo Escárate C. Characterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and non-coding RNA interplay. Mar Genomics. 2019;: pubmed publisher
    ..Target prediction and expression patterns suggested a pivotal role of miRNAs in the reproductive development of L. salmonis. ..
  3. Gonçalves A, Núñez Acuña G, Detree C, Gallardo Escárate C. Coding/non-coding cross-talk in intestinal epithelium transcriptome gives insights on how fish respond to stocking density. Comp Biochem Physiol Part D Genomics Proteomics. 2019;29:14-23 pubmed publisher
    ..This study suggests a cross-talk between coding and non-coding RNAs in the intestine of fish exposed to suboptimal conditions, providing new insights into the regulatory role of the lncRNAs on fish response to stressors. ..
  4. Detree C, Gallardo Escárate C. Single and repetitive microplastics exposures induce immune system modulation and homeostasis alteration in the edible mussel Mytilus galloprovincialis. Fish Shellfish Immunol. 2018;83:52-60 pubmed publisher
  5. Valenzuela Miranda D, Nuñez Acuña G, Valenzuela Muñoz V, Asgari S, Gallardo Escárate C. MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): emerging role in delousing drug response. Gene. 2015;555:231-41 pubmed publisher
    ..Our results suggest novel insights into the regulatory network of the post-transcriptional gene regulation in C. rogercresseyi mediated by miRNAs, evidencing a putative role during the ontogeny and drug response. ..
  6. Farlora R, Nuñez Acuña G, Gallardo Escárate C. Prohibitin-2 gene reveals sex-related differences in the salmon louse Caligus rogercresseyi. Gene. 2015;564:73-80 pubmed publisher
    ..These results contribute to expand the present knowledge of the reproduction-related genes in C. rogercresseyi, and may be useful in future experiments aimed at controlling the impacts of sea lice in fish farming. ..
  7. Núñez Acuña G, Gallardo Escárate C. Two novel male-associated peroxinectin genes are downregulated by exposure to delousing drugs in Caligus rogercresseyi. Gene. 2015;557:98-102 pubmed publisher
    ..Both PX transcripts were highly associated with male adults, and transcription levels were significantly reduced by deltamethrin and azamethiphos. This result suggests a modulation of peroxinectin in response to delousing drugs. ..
  8. Valenzuela Miranda D, Del Río Portilla M, Gallardo Escárate C. Characterization of the growth-related transcriptome in California red abalone (Haliotis rufescens) through RNA-Seq analysis. Mar Genomics. 2015;24 Pt 3:199-202 pubmed publisher
    ..These results provide a valuable resource of novel transcripts in this species and further understandings of the molecular bases regulating growth traits in H. rufescens. ..
  9. Astuya A, Carrera C, Ulloa V, Aballay A, Núñez Acuña G, Hégaret H, et al. Saxitoxin Modulates Immunological Parameters and Gene Transcription in Mytilus chilensis Hemocytes. Int J Mol Sci. 2015;16:15235-50 pubmed publisher
    ..These results suggest that STX can negatively affect the immunocompetence of M. chilensis hemocytes, which were capable of responding to STX exposure in vitro by increasing the mRNA levels of antioxidant enzymes. ..

More Information


  1. Haye P, Gallardo Escárate C. Transcriptome characterization of the ascidian Pyura chilensis using 454-pyrosequencing data from two distant localities on the southeast Pacific. Mar Genomics. 2015;20:19-22 pubmed publisher
  2. Chovar Vera O, Valenzuela Muñoz V, Gallardo Escárate C. Molecular characterization of collagen IV evidences early transcription expression related to the immune response against bacterial infection in the red abalone (Haliotis rufescens). Fish Shellfish Immunol. 2015;42:241-8 pubmed publisher
    ..The results obtained in this study propose a putative biological function for collagen IV involved in the early innate immune response of the red abalone H. rufescens. ..
  3. Maldonado Aguayo W, Lafarga De La Cruz F, Gallardo Escárate C. Identification and expression of antioxidant and immune defense genes in the surf clam Mesodesma donacium challenged with Vibrio anguillarum. Mar Genomics. 2015;19:65-73 pubmed publisher
    ..05). These results suggest that superoxide dismutase, catalase, ferritin and filamin, could be contributing to the innate immune response of M. donacium against the pathogen V. anguillarum. ..
  4. Valenzuela Muñoz V, Valenzuela Miranda D, Gallardo Escárate C. Comparative analysis of long non-coding RNAs in Atlantic and Coho salmon reveals divergent transcriptome responses associated with immunity and tissue repair during sea lice infestation. Dev Comp Immunol. 2018;87:36-50 pubmed publisher
    ..This study revealed high regulation of lncRNAs closely localized to immune and tissue repair-related genes in Atlantic and Coho salmon, respectively, suggesting putative roles for lncRNAs in salmon against sea lice infestation. ..
  5. Tapia F, Gallardo Escárate C. Spatio-temporal transcriptome analysis in the marine snail Tegula atra along central-northern Chile (28-31°S). Mar Genomics. 2015;23:61-5 pubmed publisher
    ..This transcriptome response or "frontloading" strategy can significantly increase the speed of response to thermal stress, and also be a relevant molecular underpinning to explain the genomic diversity along the Chilean coast. ..
  6. Valenzuela Miranda D, Cabrejos M, Yañez J, Gallardo Escárate C. From the viral perspective: infectious salmon anemia virus (ISAV) transcriptome during the infective process in Atlantic salmon (Salmo salar). Mar Genomics. 2015;20:39-43 pubmed publisher
    ..This study offers a more comprehensive approach regarding the ISAV infective process and gives novel knowledge for its molecular detection. ..
  7. Valenzuela Miranda D, Gallardo Escárate C, Valenzuela Muñoz V, Farlora R, Gajardo G. Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana. Mar Genomics. 2014;18PB:151-154 pubmed publisher
    ..Furthermore, 221 and 534 putative SNPs were identified exclusively in males and females, respectively. These results will build the foundation for further genomic studies in A. franciscana. ..
  8. Núñez Acuña G, Gonçalves A, Valenzuela Muñoz V, Pino Marambio J, Wadsworth S, Gallardo Escárate C. Transcriptome immunomodulation of in-feed additives in Atlantic salmon Salmo salar infested with sea lice Caligus rogercresseyi. Fish Shellfish Immunol. 2015;47:450-60 pubmed publisher
    ..This is the first published study that evaluates the transcriptomic response of sea lice-infested Atlantic salmon fed with in-feed additives. ..
  9. Boltaña S, Chávez Mardones J, Valenzuela Muñoz V, Gallardo Escárate C. Evidence for the Induction of Key Components of the NOTCH Signaling Pathway via Deltamethrin and Azamethiphos Treatment in the Sea Louse Caligus rogercresseyi. Int J Mol Sci. 2016;17: pubmed publisher
    ..rogercresseyi resulting from the frequent use of organophosphates and pyrethroids. ..
  10. Núñez Acuña G, Boltaña S, Gallardo Escárate C. Pesticides Drive Stochastic Changes in the Chemoreception and Neurotransmission System of Marine Ectoparasites. Int J Mol Sci. 2016;17: pubmed publisher
    ..These data suggest possible new targets for xenobiotic drugs that play key roles in the delousing effects of antiparasitics in sea lice. ..
  11. Vera Bizama F, Valenzuela Muñoz V, Gonçalves A, Marambio J, Hawes C, Wadsworth S, et al. Transcription expression of immune-related genes from Caligus rogercresseyi evidences host-dependent patterns on Atlantic and coho salmon. Fish Shellfish Immunol. 2015;47:725-31 pubmed publisher
    ..This study evidences transcriptome differences between two salmon host species and provides pivotal knowledge towards elaborating future control strategies. ..
  12. Farlora R, Valenzuela Muñoz V, Chávez Mardones J, Gallardo Escárate C. Aquaporin family genes exhibit developmentally-regulated and host-dependent transcription patterns in the sea louse Caligus rogercresseyi. Gene. 2016;585:119-127 pubmed publisher
    ..The observed differences in the regulation of aquaporin genes may reveal osmoregulatory adaptations associated with nutrient ingestion and metabolite waste export, exposing complex host-parasite relationships in C. rogercresseyi. ..
  13. Chávez Mardones J, Gallardo Escárate C. Next-Generation Transcriptome Profiling of the Salmon Louse Caligus rogercresseyi Exposed to Deltamethrin (AlphaMax™): Discovery of Relevant Genes and Sex-Related Differences. Mar Biotechnol (NY). 2015;17:793-810 pubmed publisher
    ..The present study reveals that deltamethrin generates a complex transcriptome response in C. rogercresseyi, thus providing valuable genomic information for developing new delousing drugs. ..
  14. Valenzuela Muñoz V, Gallardo Escárate C. Transcriptome mining: Multigene panel to test delousing drug response in the sea louse Caligus rogercresseyi. Mar Genomics. 2016;25:103-113 pubmed publisher
    ..The present study provides a multigene panel to test delousing drug response to pyrethroids and organophosphates in a highly prevalent pathogen of the Chilean salmon industry. ..
  15. Boltaña S, Sanchez M, Valenzuela V, Gallardo Escárate C. Density-dependent effects of Caligus rogercresseyi infestation on the immune responses of Salmo salar. Fish Shellfish Immunol. 2016;59:365-374 pubmed publisher
    ..This study highlights the molecular signatures for distinct parasitic loads, providing new perspectives towards fully understanding parasite-host interactions. ..
  16. Valenzuela Miranda D, Gallardo Escárate C. Novel insights into the response of Atlantic salmon (Salmo salar) to Piscirickettsia salmonis: Interplay of coding genes and lncRNAs during bacterial infection. Fish Shellfish Immunol. 2016;59:427-438 pubmed publisher
    ..These results represent the first comprehensive study of putative interplaying mechanisms of coding and non-coding RNAs during bacterial infection in salmonids. ..
  17. Valenzuela Muñoz V, Gallardo Escárate C. Iron metabolism modulation in Atlantic salmon infested with the sea lice Lepeophtheirus salmonis and Caligus rogercresseyi: A matter of nutritional immunity?. Fish Shellfish Immunol. 2017;60:97-102 pubmed publisher
    ..salmonis than C. rogercresseyi. Feeding behavior, for instance haematophagy, of the infesting sea lice species in relation to iron modulation is discussed. ..
  18. Valenzuela Muñoz V, Sturm A, Gallardo Escárate C. Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi. Parasit Vectors. 2015;8:209 pubmed publisher
    ..This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry. ..
  19. Farlora R, Araya Garay J, Gallardo Escárate C. Discovery of sex-related genes through high-throughput transcriptome sequencing from the salmon louse Caligus rogercresseyi. Mar Genomics. 2014;15:85-93 pubmed publisher
    ..This transcriptome dataset provides a useful resource for future functional analyses, opening new opportunities for sea lice pest control. ..
  20. Valenzuela Miranda D, Valenzuela Muñoz V, Farlora R, Gallardo Escárate C. MicroRNA-based transcriptomic responses of Atlantic salmon during infection by the intracellular bacterium Piscirickettsia salmonis. Dev Comp Immunol. 2017;77:287-296 pubmed publisher
  21. Valenzuela Muñoz V, Novoa B, Figueras A, Gallardo Escárate C. Modulation of Atlantic salmon miRNome response to sea louse infestation. Dev Comp Immunol. 2017;76:380-391 pubmed publisher
    ..This study contributes knowledge about the immune response of Atlantic salmon during infestation with sea lice. ..
  22. Tarifeño Saldivia E, Valenzuela Miranda D, Gallardo Escárate C. In the shadow: The emerging role of long non-coding RNAs in the immune response of Atlantic salmon. Dev Comp Immunol. 2017;73:193-205 pubmed publisher
  23. Boltaña S, Castellana B, Goetz G, Tort L, Teles M, Mulero V, et al. Extending Immunological Profiling in the Gilthead Sea Bream, Sparus aurata, by Enriched cDNA Library Analysis, Microarray Design and Initial Studies upon the Inflammatory Response to PAMPs. Int J Mol Sci. 2017;18: pubmed publisher
    ..We have developed and validated an oligonucleotide microarray (SAQ) that provides a platform enriched for the study of gene expression in S. aurata with an emphasis upon immunity and the immune response. ..
  24. Detree C, Núñez Acuña G, Tapia F, Gallardo Escárate C. Long non-coding RNAs are associated with spatiotemporal gene expression profiles in the marine gastropod Tegula atra. Mar Genomics. 2017;33:39-45 pubmed publisher
  25. Vorphal M, Gallardo Escárate C, Valenzuela Muñoz V, Dagnino Leone J, Vásquez J, Martinez Oyanedel J, et al. De novo transcriptome analysis of the red seaweed Gracilaria chilensis and identification of linkers associated with phycobilisomes. Mar Genomics. 2017;31:17-19 pubmed publisher
    ..chilensis. This resource will also provide the basis for the study of metabolic pathways related to polysaccharide production. ..
  26. Valenzuela Muñoz V, Boltaña S, Gallardo Escárate C. Comparative immunity of Salmo salar and Oncorhynchus kisutch during infestation with the sea louse Caligus rogercresseyi: An enrichment transcriptome analysis. Fish Shellfish Immunol. 2016;59:276-287 pubmed publisher
    ..Additionally, putative roles of TLRs in salmonids against sea lice were evidenced. This study is the first comparative transcriptome analysis that reveals species-specific immune responses in salmons infected with C. rogercresseyi. ..
  27. Valenzuela Miranda D, Boltaña S, Cabrejos M, Yáñez J, Gallardo Escárate C. High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues. Fish Shellfish Immunol. 2015;45:367-77 pubmed publisher