Hualan Chen



  1. Zhu P, Liang L, Shao X, Luo W, Jiang S, Zhao Q, et al. Host Cellular Protein TRAPPC6AΔ Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. J Virol. 2017;91: pubmed publisher
    ..Importantly, we discovered that TRAPPC6AΔ can positively regulate viral replication in vitro by modulating M2 trafficking to the plasma membrane. ..
  2. Ding L, Chen P, Bao X, Li A, Jiang Y, Hu Y, et al. Recombinant duck enteritis viruses expressing the Newcastle disease virus (NDV) F gene protects chickens from lethal NDV challenge. Vet Microbiol. 2019;232:146-150 pubmed publisher
    ..rDEV-F may therefore serve as a promising vaccine candidate for chickens. This is the first report of a DEV-vectored vaccine providing robust protection against lethal NDV infection in chickens. ..
  3. Gu C, Zeng X, Song Y, Li Y, Liu L, Kawaoka Y, et al. Glycosylation and an amino acid insertion in the head of hemagglutinin independently affect the antigenic properties of H5N1 avian influenza viruses. Sci China Life Sci. 2019;62:76-83 pubmed publisher
    ..We also found that the amino acid N131 in the H5 HA protein is glycosylated. Our results provide experimental evidence that glycosylation and an amino acid insertion or deletion in HA influence antigenic variation. ..
  4. Zeng X, Tian G, Shi J, Deng G, Li C, Chen H. Vaccination of poultry successfully eliminated human infection with H7N9 virus in China. Sci China Life Sci. 2018;61:1465-1473 pubmed publisher
    ..These facts emphasize that active control of animal disease is extremely important for zoonosis control and human health protection. ..
  5. Yang H, Xiao Y, Meng F, Sun F, Chen M, Cheng Z, et al. Emergence of H3N8 equine influenza virus in donkeys in China in 2017. Vet Microbiol. 2018;214:1-6 pubmed publisher
    ..This study provides insight into the currently circulating and newly emerging H3N8 strains in donkeys in China. ..
  6. Shi J, Deng G, Ma S, Zeng X, Yin X, Li M, et al. Rapid Evolution of H7N9 Highly Pathogenic Viruses that Emerged in China in 2017. Cell Host Microbe. 2018;24:558-568.e7 pubmed publisher
    ..Additionally, some H7N9 and H7N2 viruses carrying duck virus genes are lethal in ducks. Thus, although vaccination reduced H7N9 infections, the increased virulence and expanded host range to ducks pose new challenges. ..
  7. Yang W, Yin X, Guan L, Li M, Ma S, Shi J, et al. A live attenuated vaccine prevents replication and transmission of H7N9 highly pathogenic influenza viruses in mammals. Emerg Microbes Infect. 2018;7:153 pubmed publisher
    ..Our study suggests that H7N9/AAca has the potential to be an effective H7N9 vaccine and should be evaluated in humans. ..
  8. Li X, Liu B, Ma S, Cui P, Liu W, Li Y, et al. High frequency of reassortment after co-infection of chickens with the H4N6 and H9N2 influenza A viruses and the biological characteristics of the reassortants. Vet Microbiol. 2018;222:11-17 pubmed publisher
    ..These results highlight the potential public risk of the avian influenza reassortants and the importance of surveillance of the co-existence of the H4N6 and H9N2 viruses in avian species and other animals. ..
  9. Xu X, Bao H, Ma Y, Sun J, Zhao Y, Wang Y, et al. Simultaneous detection of novel H7N9 and other influenza A viruses in poultry by multiplex real-time RT-PCR. Virol J. 2015;12:69 pubmed publisher
    ..The multiplex rRT-PCR assay in this study has good specificity, sensitivity and reproducibility, and will be useful for laboratory surveillance and rapid diagnosis of H7N9 and other influenza A viruses. ..

More Information


  1. Yang H, Chen Y, Qiao C, Xu C, Yan M, Xin X, et al. Two different genotypes of H1N2 swine influenza virus isolated in northern China and their pathogenicity in animals. Vet Microbiol. 2015;175:224-31 pubmed publisher
    ..Based on the results, it is possible that the new genotype of the swine H1N2 virus that emerged in China may become widespread in the swine population and pose a potential threat to public health. ..
  2. Bao H, Ma Y, Shi J, Zeng X, Zhao Y, Wang X, et al. Evaluation and application of a one-step duplex real-time reverse transcription polymerase chain reaction assay for the rapid detection of influenza A (H7N9) virus from poultry samples. Arch Virol. 2015;160:2471-7 pubmed publisher
  3. Qiao C, Liu L, Yang H, Chen Y, Xu H, Chen H. Novel triple reassortant H1N2 influenza viruses bearing six internal genes of the pandemic 2009/H1N1 influenza virus were detected in pigs in China. J Clin Virol. 2014;61:529-34 pubmed publisher
    ..Influenza virus reassortment is an ongoing process, and our findings highlight the urgent need for continued influenza surveillance among swine herds. ..
  4. Deng G, Shi J, Wang J, Kong H, Cui P, Zhang F, et al. Genetics, Receptor Binding, and Virulence in Mice of H10N8 Influenza Viruses Isolated from Ducks and Chickens in Live Poultry Markets in China. J Virol. 2015;89:6506-10 pubmed publisher
    ..The viruses bound to both human- and avian-type receptors, and four of the viruses caused 12.7% to 22.5% body weight loss in mice. ..
  5. Liu L, Zeng X, Chen P, Deng G, Li Y, Shi J, et al. Characterization of Clade 7.2 H5 Avian Influenza Viruses That Continue To Circulate in Chickens in China. J Virol. 2016;90:9797-9805 pubmed publisher
    ..2 viruses still exclusively bind to avian-type receptors and are of low pathogenicity to mice, suggesting that these H5 viruses pose a low risk to human public health. ..
  6. Feng X, Wang Z, Shi J, Deng G, Kong H, Tao S, et al. Glycine at Position 622 in PB1 Contributes to the Virulence of H5N1 Avian Influenza Virus in Mice. J Virol. 2016;90:1872-9 pubmed publisher
    ..This newly identified virulence-related marker could be applied to the development of live attenuated vaccines against H5N1 influenza. ..
  7. Li X, Fu Y, Yang J, Guo J, He J, Guo J, et al. Genetic and biological characterization of two novel reassortant H5N6 swine influenza viruses in mice and chickens. Infect Genet Evol. 2015;36:462-466 pubmed publisher
    ..However, they might keep evolving and pose a potential risk to public health and the continued surveillance of swine influenza should be strengthened. ..
  8. Wen X, Sun J, Wang X, Bao H, Zhao Y, Zeng X, et al. Identification of a novel linear epitope on the NS1 protein of avian influenza virus. BMC Microbiol. 2015;15:168 pubmed publisher
    ..This MAb and its epitope may facilitate future studies on NS1 function and aid the development of new diagnostic methods for AIV detection. ..
  9. Qu Z, Ma S, Kong H, Deng G, Shi J, Liu L, et al. Identification of a key amino acid in hemagglutinin that increases human-type receptor binding and transmission of an H6N2 avian influenza virus. Microbes Infect. 2017;19:655-660 pubmed publisher
  10. Cui P, Deng G, Shi J, Kong H, Liu L, Guan Y, et al. New influenza A(H7N7) viruses detected in live poultry markets in China. Virology. 2016;499:165-169 pubmed publisher
    ..It is important to continue to monitor the evolution of H7N7 viruses and to evaluate their potential to cause human infections. ..
  11. Zhao D, Liang L, Wang S, Nakao T, Li Y, Liu L, et al. Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response. J Virol. 2017;91: pubmed publisher
    ..This glycosylation event enhanced viral productivity, exacerbated the host response, and thereby contributed to the high pathogenicity of H5N1 virus in mice. ..
  12. Ge Y, Chai H, Fan Z, Wang X, Yao Q, Ma J, et al. New H6 influenza virus reassortment strains isolated from Anser fabalis in Anhui Province, China. Virol J. 2017;14:36 pubmed publisher
    ..Our findings suggest that regular surveillance of wild birds, especially migratory birds, is important for providing early warning and control of avian influenza outbreaks. ..
  13. Wang Z, Yang H, Chen Y, Tao S, Liu L, Kong H, et al. A Single-Amino-Acid Substitution at Position 225 in Hemagglutinin Alters the Transmissibility of Eurasian Avian-Like H1N1 Swine Influenza Virus in Guinea Pigs. J Virol. 2017;91: pubmed publisher
  14. Ma Y, Sun J, Gu L, Bao H, Zhao Y, Shi L, et al. Annexin A2 (ANXA2) interacts with nonstructural protein 1 and promotes the replication of highly pathogenic H5N1 avian influenza virus. BMC Microbiol. 2017;17:191 pubmed publisher
    ..Our results indicate that ANXA2 interacts with NS1 and ANXA2 expression increases HPAIV replication. ..
  15. Sui J, Yang D, Qiao C, Xu H, Xu B, Wu Y, et al. Protective efficacy of an inactivated Eurasian avian-like H1N1 swine influenza vaccine against homologous H1N1 and heterologous H1N1 and H1N2 viruses in mice. Vaccine. 2016;34:3757-63 pubmed publisher
    ..Our findings suggest that this EA H1N1 vaccine can provide protection against both homologous H1N1 and heterologous H1N1 or H1N2 virus infection. As such, it is an excellent vaccine candidate to prevent H1N1 swine influenza. ..