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Genomes and Genes | Raymund J WellingerSummaryCountry: Canada Publications
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Publications
When the caps fall off: responses to telomere uncapping in yeastRaymund J Wellinger
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
FEBS Lett 584:3734-40. 2010..I will provide an outline of cellular responses to uncapping in budding yeast and briefly discuss the reverse, namely avoidance mechanisms that prevent telomere formation at inappropriate places...- The CST complex and telomere maintenance: the exception becomes the ruleRaymund J Wellinger
Department of Microbiology and Infectious Diseases, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Mol Cell 36:168-9. 2009..In this issue of Molecular Cell, Miyake et al. (2009) and Surovtseva et al. (2009) provide evidence that these complexes coexist and function at telomeres in many species... 
Telomerase- and capping-independent yeast survivors with alternate telomere statesMichel Larrivée
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada
Nat Cell Biol 8:741-7. 2006..Chromosome capping, therefore, is not strictly dependent on canonical capping proteins, such as Cdc13p, but can be achieved by alternate mechanisms...
Telomere capping in non-dividing yeast cells requires Yku and Rap1Momchil D Vodenicharov
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
EMBO J 29:3007-19. 2010..Together, our results show a high flexibility of the telomeric cap and suggest that distinct configurations may provide for efficient capping in dividing versus non-dividing cells...- Differential participation of homologous recombination and nucleotide excision repair in yeast survival to ultraviolet light radiationMartin Toussaint
Departement de Microbiologie et d Infectiologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Mutat Res 698:52-9. 2010..Additional aims were to study the participation of HR and NER in promoting UV survival during the cell cycle, and to define the extent of the co-participation for both repair pathways... - Introns within ribosomal protein genes regulate the production and function of yeast ribosomesJulie Parenteau
Laboratoire de génomique fonctionnelle de l Université de Sherbrooke, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Quebec, Canada
Cell 147:320-31. 2011..Together, our results indicate that splicing in yeast RP genes mediates intergene regulation and implicate the expression ratio of duplicated RP genes in modulating ribosome function... - RNase III-dependent regulation of yeast telomeraseStephanie Larose
RNA Group, Departement de Microbiologie et d Infectiologie, Facultéde Médecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
J Biol Chem 282:4373-81. 2007..These results reveal a new mechanism that influences telomeres length by controlling the expression of the telomerase subunits... - Budding yeast telomerase RNA transcription termination is dictated by the Nrd1/Nab3 non-coding RNA termination pathwayJean François Noël
RNA Group, Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, 3001, 12e Ave Nord, Sherbrooke, Quebec, J1H 5N4, Canada
Nucleic Acids Res 40:5625-36. 2012.... - Assessing telomeric phenotypesCatherine Lebel
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Quebec, Canada
Methods Mol Biol 313:265-316. 2006..However, growth phenotypes (senescence) and fine-structure analyses of the chromosome terminal DNA are also becoming increasingly important... - Abrupt telomere losses and reduced end-resection can explain accelerated senescence of Smc5/6 mutants lacking telomeraseJean François Noël
Dept of Microbiology and Infectious Diseases, Faculty of Medicine, University of Sherbrooke, 3001, 12e Ave Nord, Sherbrooke, Qc J1J 1G2, Canada
DNA Repair (Amst) 10:271-82. 2011.... - DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinaseMomchil D Vodenicharov
Department of Microbiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
Mol Cell 24:127-37. 2006..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts... - Telomerase is required to protect chromosomes with vertebrate-type T2AG3 3' ends in Saccharomyces cerevisiaeAmadou Bah
Departement de Microbiologie et d Infectiologie, Groupe ARN RNA Group, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
J Biol Chem 286:27132-8. 2011..These results show that in budding yeast the integrity of the 3' overhang is required for proper termination of telomere replication as well as protection... - A mutation in yeast Tel1p that causes differential effects on the DNA damage checkpoint and telomere maintenanceMikhail Chakhparonian
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, QC, J1H 5N4, Canada
Curr Genet 48:310-22. 2005..The results thus strongly suggest that the different cellular pathways affected by Tel1p do not require the same level of Tel1p activity to be fully functional... - The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complexMichel Larrivée
Departement De Microbiologie et Infectiologie, Groupe ARN RNA Group, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, J1H 5N4 Canada
Genes Dev 18:1391-6. 2004..Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment... - The cell division cycle puts up with unprotected telomeres: cell cycle regulated telomere uncapping as a means to achieve telomere homeostasisMomchil D Vodenicharov
Department of Microbiology, Faculty of Medicine, Universite, de Sherbrooke, Sherbrooke, Quebec, Canada
Cell Cycle 6:1161-7. 2007..Thus, a temporally limited state of telomere dysfunction leading to chromosome end processing may represent a well-governed cell cycle event that constitutes an integral part of the assembly of a new functional telomere... - Genome-wide mapping of DNA strand breaksFrederic Leduc
Departement de Biochimie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
PLoS ONE 6:e17353. 2011..This mapping technique could find several applications for instance in the study of aging, genotoxic drug screening, cancer, meiosis, radiation and oxidative DNA damage... - Two-dimensional agarose gel analysis of DNA replication intermediatesAlain T Dandjinou
Department of Medical Microbiology and Infectious Diseases, Faculty of Medicine, , Quebec, Canada
Methods Mol Biol 313:193-208. 2006..The 2-D gel is then blotted and probed to allow an examination of replication intermediates in specific DNA regions... - UV-induced DNA damage and DNA repair in ribosomal genes chromatinJulie Pelloux
Departement De Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, QC, Canada
Methods Mol Biol 809:303-20. 2012..Using this approach, we have shown that NER is faster in nonnucleosomes vs. nucleosomes rDNA, that the formation of CPDs promotes changes in the active rDNA chromatin, and that NER is coupled to rRNA genes transcription... - TLC1 RNA nucleo-cytoplasmic trafficking links telomerase biogenesis to its recruitment to telomeresFranck Gallardo
Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada
EMBO J 27:748-57. 2008..Altogether, our results reveal that the nucleo-cytoplasmic trafficking of the TLC1 RNA is an important step in telomere homeostasis, and link telomerase biogenesis to its recruitment to telomeres... - Telomeres: what's new at your end?Catherine Lebel
Department of Microbiology and Infectious Diseases, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
J Cell Sci 118:2785-8. 2005 - Deletion of many yeast introns reveals a minority of genes that require splicing for functionJulie Parenteau
Laboratoire de génomique fonctionnelle de l Université de Sherbrooke, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Mol Biol Cell 19:1932-41. 2008.... - Alternative splicing of SYK regulates mitosis and cell survivalPanagiotis Prinos
Laboratoire de Génomique Fonctionnelle, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
Nat Struct Mol Biol 18:673-9. 2011..The data suggest that splicing of selected genes is specifically modified during tumor development to allow the expression of isoforms that promote cancer cell survival... - A new link for a linker histoneAntonio Conconi
, , Sherbrooke, Quebec, J1H 5N4 Canada
Mol Cell 11:1421-3. 2003..Remarkably, yeast histone H1 may not comply, as it appears to regulate homologous recombination specifically... - A high-throughput method to measure the sensitivity of yeast cells to genotoxic agents in liquid culturesMartin Toussaint
, , , Sherbrooke, Que. J1H 5N4, Canada
Mutat Res 606:92-105. 2006..This new protocol is sensitive, provides quantifiable data, offers increased screening capability and speed compared to the colony formation test... - Humanized telomeres and an attempt to express a functional human telomerase in yeastAmadou Bah
Department of Microbiology and Infectiology, ARN/RNA Group, Faculty of Medicine, , Sherbrooke, Canada
Nucleic Acids Res 32:1917-27. 2004..The results suggest that vertebrate repeats on yeast telomeres are subject to a very high degree of repeat turnover and show that an innermost tract of 50 bp of yeast repeats are resistant to replacement... - Maintenance of double-stranded telomeric repeats as the critical determinant for cell viability in yeast cells lacking KuSerge Gravel
Department of Microbiology, RNA Group/Groupe ARN, Faculty of Medicine, , Sherbrooke, Quebec J1H 5N4, Canada
Mol Cell Biol 22:2182-93. 2002..Thus, we hypothesize that an aspect of the coordinated synthesis of double-stranded telomeric repeats is sensitive to elevated temperatures... - Limited TTP supply affects telomere length regulation in a telomerase-independent fashionMartin Toussaint
Department of Microbiology and Infectious Diseases, Faculty of Medicine, , 12 Ave Nord, Sherbrooke, , J1H 5N4, Canada
Nucleic Acids Res 33:704-13. 2005..These results thus re-emphasize the importance of the interplay between conventional replication and telomerase-mediated addition of telomeric repeats in telomere replication... - Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to endRaymund J Wellinger
Department of Microbiology and Infectiology, RNA Group, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Genetics 191:1073-105. 2012..We present a comprehensive review of yeast telomere biology that covers capping, replication, recombination, and transcription. We think of it as yeast telomeres--soup to nuts... - Telomere maintenance and DNA replication: how closely are these two connected?Mikhail Chakhparonian
Department of Microbiology and Infectiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12th Ave N, Sherbrooke QC, Canada J1H 5N4
Trends Genet 19:439-46. 2003.... - Targeting heterogeneous nuclear ribonucleoparticule A1 and A2 proteins by RNA interference promotes cell death in transformed but not in normal mouse cell linesCaroline Patry
, , , 3001 12th Avenue Nord, Sherbrooke, Quebec J1H 5N4, Canada
Mol Cancer Ther 3:1193-9. 2004..These results firmly establish A1 and A2 as proteins required for the viability of transformed murine and human cells, irrespective of the status of telomerase expression or the length of the double-stranded telomeric repeat... - Exposing secrets of telomere-telomerase encountersJean François Noël
Department of Microbiology and Infectiology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada
Cell 150:453-4. 2012..In human cells, this interaction is mediated by Cajal bodies as matchmaker, and now Zhong et al. reveal molecular determinants that establish good chemistry between the two partners... - A phylogenetically based secondary structure for the yeast telomerase RNAAlain T Dandjinou
Groupe ARN/RNA Group, Department of Microbiology and Infectious Diseases, Faculty of Medicine, , 3001 12th Avenue N, Sherbrooke, QC J1H 5N4, Canada
Curr Biol 14:1148-58. 2004..The predicted structure now allows for a detailed and rationally designed study to the structure-function relationships within the telomerase RNP-complex in a genetically tractable system... - [The structures at the termini of chromosomes: what is there hidden under the cap ?]Catherine Lebel
Departement de Microbiologie et d Infectiologie, Faculte de Medecine, Universite de Sherbrooke, 3001, 12e Avenue Nord, Sherbrooke, Quebec, J1H 5N4, Canada
Med Sci (Paris) 20:207-12. 2004..Therefore, telomeres, and particularly their terminal DNA structures, are critical for the integrity of the genome... - Identification of alternative splicing markers for breast cancerJulian P Venables
Laboratoire de génomique fonctionnelle de l Université de Sherbrooke, Quebec, Canada
Cancer Res 68:9525-31. 2008..These results provide a simple alternative for the classification of normal and cancerous breast tumor tissues and underscore the putative role of alternative splicing in the biology of cancer...