Silke Wiesner

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. ncbi Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain
    Silke Wiesner
    Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Cell 130:651-62. 2007
  2. ncbi The ubiquitin binding region of the Smurf HECT domain facilitates polyubiquitylation and binding of ubiquitylated substrates
    Abiodun A Ogunjimi
    Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    J Biol Chem 285:6308-15. 2010
  3. ncbi Structural studies of FF domains of the transcription factor CA150 provide insights into the organization of FF domain tandem arrays
    James M Murphy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Mol Biol 393:409-24. 2009
  4. ncbi An exchange-free measure of 15N transverse relaxation: an NMR spectroscopy application to the study of a folding intermediate with pervasive chemical exchange
    D Flemming Hansen
    Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada, M5S 1A8
    J Am Chem Soc 129:11468-79. 2007
  5. ncbi A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases
    Silke Wiesner
    Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada
    EMBO J 25:4686-96. 2006
  6. ncbi Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins
    Patrik Lundström
    Department of Medical Genetics, The University of Toronto, Toronto, ON, Canada, M5S 1A8
    J Biomol NMR 38:199-212. 2007
  7. ncbi The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains
    Alexander Gasch
    Structural Biology Program, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:356-64. 2006
  8. ncbi NMR Assignment Reveals an alpha-Helical Fold for the F-Actin Binding Domain of Human Bcr-Abl/c-Abl
    Silke Wiesner
    J Biomol NMR 32:335. 2005
  9. ncbi Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl
    Oliver Hantschel
    Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 19/3, 1090 Vienna, Austria
    Mol Cell 19:461-73. 2005
  10. ncbi Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40
    Silke Wiesner
    Structural Biology Programme, EMBL Heidelberg, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Mol Biol 324:807-22. 2002

Collaborators

Detail Information

Publications12

  1. ncbi Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain
    Silke Wiesner
    Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Cell 130:651-62. 2007
    ..Thus, interactions between C2 and HECT domains autoinhibit a subset of HECT-type E3s to protect them and their substrates from futile degradation in cells...
  2. ncbi The ubiquitin binding region of the Smurf HECT domain facilitates polyubiquitylation and binding of ubiquitylated substrates
    Abiodun A Ogunjimi
    Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    J Biol Chem 285:6308-15. 2010
    ..Our findings suggest the UBS promotes polyubiquitylation by stabilizing ubiquitylated substrate binding to the HECT domain...
  3. ncbi Structural studies of FF domains of the transcription factor CA150 provide insights into the organization of FF domain tandem arrays
    James M Murphy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Mol Biol 393:409-24. 2009
    ....
  4. ncbi An exchange-free measure of 15N transverse relaxation: an NMR spectroscopy application to the study of a folding intermediate with pervasive chemical exchange
    D Flemming Hansen
    Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada, M5S 1A8
    J Am Chem Soc 129:11468-79. 2007
    ....
  5. ncbi A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases
    Silke Wiesner
    Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada
    EMBO J 25:4686-96. 2006
    ....
  6. ncbi Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins
    Patrik Lundström
    Department of Medical Genetics, The University of Toronto, Toronto, ON, Canada, M5S 1A8
    J Biomol NMR 38:199-212. 2007
    ..The utility of the labeling is established by recording (13)C R (1rho) and CPMG-based experiments on a number of different protein systems...
  7. ncbi The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains
    Alexander Gasch
    Structural Biology Program, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:356-64. 2006
    ..Our results also revealed that not all FF domains in Prp40 are functionally equivalent. We proposed that at least two different interaction surfaces exist in FF domains that have evolved to recognize distinct binding motifs...
  8. ncbi NMR Assignment Reveals an alpha-Helical Fold for the F-Actin Binding Domain of Human Bcr-Abl/c-Abl
    Silke Wiesner
    J Biomol NMR 32:335. 2005
  9. ncbi Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl
    Oliver Hantschel
    Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 19/3, 1090 Vienna, Austria
    Mol Cell 19:461-73. 2005
    ..We propose that these interactions represent a major determinant for both Bcr-Abl and c-Abl localization...
  10. ncbi Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40
    Silke Wiesner
    Structural Biology Programme, EMBL Heidelberg, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Mol Biol 324:807-22. 2002
    ..In contrast, no interaction was observed between the Prp40 WW domains and the CTD repeats used in this work...
  11. ncbi Interactions between the three CIN85 SH3 domains and ubiquitin: implications for CIN85 ubiquitination
    Irina Bezsonova
    Department of Chemistry, University of Toronto, 80 St George Street, Toronto, ON, Canada M5S 1A8
    Biochemistry 47:8937-49. 2008
    ..These results suggest that competition between Cbl and ubiquitin binding to CIN85 regulates Cbl function and EGFR endocytosis...
  12. ncbi WW and SH3 domains, two different scaffolds to recognize proline-rich ligands
    Maria J Macias
    Structural and Computational Biology Program, EMBL Heidelberg, Heidelberg, Germany
    FEBS Lett 513:30-7. 2002
    ..The binding surface of the modeled WW domain of Npw38 protein shows a remarkable similarity to the SH3 domain of Sem5 protein, confirming biochemical data on similar binding predilections of both domains...