C Whitfield

Summary

Affiliation: University of Guelph
Country: Canada

Publications

  1. Diao J, Bouwman C, Yan D, Kang J, Katakam A, Liu P, et al. Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate. MBio. 2017;8: pubmed publisher
  2. Doyle L, Ovchinnikova O, Whitfield C. Utilization of Fluorescently Tagged Synthetic Acceptor Molecules for In Vitro Characterization of a Dual-Domain Glycosyltransferase Enzyme, KpsC, from Escherichia coli. Methods Mol Biol. 2019;1954:151-159 pubmed publisher
    ..Here we describe an application of this technique which was previously used to functionally characterize a dual-domain glycosyltransferase enzyme, KpsC, involved in capsular polysaccharide biosynthesis in Escherichia coli. ..
  3. Williams D, Ovchinnikova O, Whitfield C. In Vitro Characterization of a Multidomain Glycosyltransferase Using Fluorescently Tagged Synthetic Acceptors. Methods Mol Biol. 2019;1954:245-253 pubmed publisher
    ..The reaction products were verified by mass spectrometry and nuclear magnetic resonance methods. ..
  4. Whitfield C, Naismith J. Periplasmic export machines for outer membrane assembly. Curr Opin Struct Biol. 2008;18:466-74 pubmed publisher
    ..New structural and biochemical information identifies an envelope-spanning molecular scaffold for the export of group 1 capsular polysaccharides and provides insight into a complex molecular machine. ..
  5. Nickerson N, Mainprize I, Hampton L, Jones M, Naismith J, Whitfield C. Trapped translocation intermediates establish the route for export of capsular polysaccharides across Escherichia coli outer membranes. Proc Natl Acad Sci U S A. 2014;111:8203-8 pubmed publisher
  6. Liston S, Clarke B, Greenfield L, Richards M, Lowary T, Whitfield C. Domain interactions control complex formation and polymerase specificity in the biosynthesis of the Escherichia coli O9a antigen. J Biol Chem. 2015;290:1075-85 pubmed publisher
    ..Complex domain interactions are needed to position the polymerase components appropriately for assembly into a functional complex located at the cytoplasmic membrane. ..
  7. Clarke B, Ovchinnikova O, Kelly S, Williamson M, BUTLER J, Liu B, et al. Molecular basis for the structural diversity in serogroup O2-antigen polysaccharides in Klebsiella pneumoniae. J Biol Chem. 2018;293:4666-4679 pubmed publisher
    ..These findings now provide a complete understanding of the molecular genetic basis for the known variations in Klebsiella O-antigen carbohydrate structures based on the O2a backbone. ..
  8. Ovchinnikova O, Doyle L, Huang B, Kimber M, Lowary T, Whitfield C. Biochemical Characterization of Bifunctional 3-Deoxy-?-d-manno-oct-2-ulosonic Acid (?-Kdo) Transferase KpsC from Escherichia coli Involved in Capsule Biosynthesis. J Biol Chem. 2016;291:21519-21530 pubmed
    ..Based on phylogenetic analyses, we propose the linkage specificities of the glycosyltransferase domains are conserved in KpsC homologs from other bacterial species. ..
  9. Williams D, Ovchinnikova O, Koizumi A, Mainprize I, Kimber M, Lowary T, et al. Single polysaccharide assembly protein that integrates polymerization, termination, and chain-length quality control. Proc Natl Acad Sci U S A. 2017;114:E1215-E1223 pubmed publisher
    ..These findings offer insight into glycan assembly mechanisms and the generation of antigenic diversity as well as potential tools for glycoengineering. ..

More Information

Publications15

  1. request reprint
    Whitfield C, Roberts I. Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol Microbiol. 1999;31:1307-19 pubmed
    ..coli into four distinct groups...
  2. request reprint
    Whitfield C. Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Annu Rev Biochem. 2006;75:39-68 pubmed
    ..These systems have an impact on both a general understanding of membrane trafficking in bacteria and on bacterial pathogenesis...
  3. Mann E, Ovchinnikova O, King J, Whitfield C. Bacteriophage-mediated Glucosylation Can Modify Lipopolysaccharide O-Antigens Synthesized by an ATP-binding Cassette (ABC) Transporter-dependent Assembly Mechanism. J Biol Chem. 2015;290:25561-70 pubmed publisher
    ..coli chromosome, restoring native level antisera sensitivity and structure. There are therefore no intrinsic mechanistic barriers for bacteriophage-mediated O-antigen glucosylation in ABC transporter-dependent pathways. ..
  4. Mann E, Mallette E, Clarke B, Kimber M, Whitfield C. The Klebsiella pneumoniae O12 ATP-binding Cassette (ABC) Transporter Recognizes the Terminal Residue of Its O-antigen Polysaccharide Substrate. J Biol Chem. 2016;291:9748-61 pubmed publisher
    ..These results demonstrate that the CBM-mediated substrate-recognition mechanism is evolutionarily conserved and can operate with glycans of widely differing structures. ..
  5. Liston S, Ovchinnikova O, Whitfield C. Unique lipid anchor attaches Vi antigen capsule to the surface of Salmonella enterica serovar Typhi. Proc Natl Acad Sci U S A. 2016;113:6719-24 pubmed publisher
    ..The structure of the lipidated terminus dictates a unique assembly mechanism and has potential implications in pathogenesis and vaccine production. ..
  6. Liston S, Mann E, Whitfield C. Glycolipid substrates for ABC transporters required for the assembly of bacterial cell-envelope and cell-surface glycoconjugates. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862:1394-1403 pubmed publisher
    ..This article is part of a Special Issue entitled: Bacterial Lipids edited by Russell E. Bishop. ..