A J Watson

Summary

Affiliation: University of Western Ontario
Country: Canada

Publications

  1. pmc Ouabain stimulates a Na+/K+-ATPase-mediated SFK-activated signalling pathway that regulates tight junction function in the mouse blastocyst
    Holly Giannatselis
    Department of Obstetrics and Gynaecology, The University of Western Ontario, London, Ontario, Canada
    PLoS ONE 6:e23704. 2011
  2. pmc Genomic RNA profiling and the programme controlling preimplantation mammalian development
    Christine E Bell
    Department of Obstetrics and Gynaecology and Physiology and Pharmacology, The University of Western Ontario, Children s Health Research Institute Lawson Health Research Institute, London, Ontario, Canada
    Mol Hum Reprod 14:691-701. 2008
  3. pmc Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation
    Barry Fong
    Department of Physiology and Pharmacology, The University of Western Ontario London, Ontario, Canada
    BMC Dev Biol 7:2. 2007
  4. ncbi request reprint Oocyte cytoplasmic maturation: a key mediator of oocyte and embryo developmental competence
    A J Watson
    Department of Obstetrics and Gynaecology, The University of Western Ontario, Children s Health Research Institute, London, Ontario, Canada N6A 4G5
    J Anim Sci 85:E1-3. 2007
  5. ncbi request reprint Molecular regulation of blastocyst formation
    A J Watson
    Department of Biochemistry and Molecular Biology, The University of Calgary, Health Sciences Centre, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Anim Reprod Sci 82:583-92. 2004
  6. ncbi request reprint Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis
    A J Watson
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 62:355-64. 2000
  7. ncbi request reprint Gene expression regulating blastocyst formation
    A J Watson
    Dept of Obstetrics and Gynaecology, The University of Western Ontario, London, Canada
    Theriogenology 51:117-33. 1999
  8. ncbi request reprint Sensitivity of bovine blastocyst gene expression patterns to culture environments assessed by differential display RT-PCR
    D R Natale
    Department of Obstetrics and Gynaecology and Department of Physiology, The University of Western Ontario, London, Ontario, Canada
    Reproduction 122:687-93. 2001
  9. ncbi request reprint The gamma-subunit of the Na-K-ATPase as a potential regulator of apical and basolateral Na+-pump isozymes during development of bovine pre-attachment embryos
    L C Barcroft
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    Reproduction 124:387-97. 2002
  10. ncbi request reprint Cyclooxygenase-2 and prostaglandin E(2)(PGE(2)) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE(2) induces moderate expansion of the bovine cumulus in vitro
    M D Calder
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 65:135-40. 2001

Collaborators

Detail Information

Publications21

  1. pmc Ouabain stimulates a Na+/K+-ATPase-mediated SFK-activated signalling pathway that regulates tight junction function in the mouse blastocyst
    Holly Giannatselis
    Department of Obstetrics and Gynaecology, The University of Western Ontario, London, Ontario, Canada
    PLoS ONE 6:e23704. 2011
    ..The results demonstrate that SFKs are expressed during preimplantation development and that SFK activity is required for blastocyst formation and is an important mediator of trophectoderm tight junction permeability...
  2. pmc Genomic RNA profiling and the programme controlling preimplantation mammalian development
    Christine E Bell
    Department of Obstetrics and Gynaecology and Physiology and Pharmacology, The University of Western Ontario, Children s Health Research Institute Lawson Health Research Institute, London, Ontario, Canada
    Mol Hum Reprod 14:691-701. 2008
    ..All of this research is relevant due to the necessity to produce healthy preimplantation embryos for embryo transfer, to ensure that assisted reproductive technologies are applied in the most efficient and safest way possible...
  3. pmc Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation
    Barry Fong
    Department of Physiology and Pharmacology, The University of Western Ontario London, Ontario, Canada
    BMC Dev Biol 7:2. 2007
    ....
  4. ncbi request reprint Oocyte cytoplasmic maturation: a key mediator of oocyte and embryo developmental competence
    A J Watson
    Department of Obstetrics and Gynaecology, The University of Western Ontario, Children s Health Research Institute, London, Ontario, Canada N6A 4G5
    J Anim Sci 85:E1-3. 2007
    ..Collectively we are beginning to specify oocyte cytoplasmic maturation, and eventually a coherent understanding of this critical event in oocyte biology will emerge...
  5. ncbi request reprint Molecular regulation of blastocyst formation
    A J Watson
    Department of Biochemistry and Molecular Biology, The University of Calgary, Health Sciences Centre, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Anim Reprod Sci 82:583-92. 2004
    ..Such studies define the physiology of blastocyst formation and serve to support the application of assisted reproductive technologies (ART) to both human and animal species...
  6. ncbi request reprint Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis
    A J Watson
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 62:355-64. 2000
    ..Our findings indicate that cSOFMaa medium is an effective base medium for bovine oocyte maturation...
  7. ncbi request reprint Gene expression regulating blastocyst formation
    A J Watson
    Dept of Obstetrics and Gynaecology, The University of Western Ontario, London, Canada
    Theriogenology 51:117-33. 1999
    ....
  8. ncbi request reprint Sensitivity of bovine blastocyst gene expression patterns to culture environments assessed by differential display RT-PCR
    D R Natale
    Department of Obstetrics and Gynaecology and Department of Physiology, The University of Western Ontario, London, Ontario, Canada
    Reproduction 122:687-93. 2001
    ..Continued research using molecular techniques will lead to increased understanding of developmental mechanisms causing culture-related phenotypic abnormalities in post-implantation embryos...
  9. ncbi request reprint The gamma-subunit of the Na-K-ATPase as a potential regulator of apical and basolateral Na+-pump isozymes during development of bovine pre-attachment embryos
    L C Barcroft
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    Reproduction 124:387-97. 2002
    ....
  10. ncbi request reprint Cyclooxygenase-2 and prostaglandin E(2)(PGE(2)) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE(2) induces moderate expansion of the bovine cumulus in vitro
    M D Calder
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 65:135-40. 2001
    ..Although the PGE(2) pathway is involved in cumulus expansion, serum factors are required to mediate PGE(2)-induced expansion...
  11. ncbi request reprint Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos
    P A De Sousa
    Department of Obstetrics and Gynaecology, University of Western Ontario, London, Canada
    Mol Reprod Dev 49:119-30. 1998
    ....
  12. ncbi request reprint Differential involvement of Na(+),K(+)-ATPase isozymes in preimplantation development of the mouse
    D J Macphee
    Department of Physiology, The University of Western Ontario, London, Ontario, N6A 5C1, Canada
    Dev Biol 222:486-98. 2000
    ..Our findings imply that the regulation of sodium transport within the preimplantation mouse embryo is more complex than had been appreciated...
  13. ncbi request reprint Regulation of blastocyst formation
    A J Watson
    Departments of Obstetrics and Gynaecology and Physiology, The University of Western Ontario, London, Ontario Canada, N6A 5C1
    Front Biosci 6:D708-30. 2001
    ..Blastocyst formation is therefore directly linked with trophectoderm cell differentiation, which arises through fundamental cell biological processes that are associated with the establishment of cell polarity...
  14. ncbi request reprint Expression of PTHrP and PTHR (PTH/PTHrP-r) mRNAs and polypeptides in bovine ovary and stimulation of bovine blastocyst development in vitro following PTHrP treatment during oocyte maturation
    P H Watson
    Department of Medicine, The University of Western Ontario, and the Lawson Research Institute, London, Canada
    Anat Embryol (Berl) 203:175-84. 2001
    ..The results suggest that granulosa cells may be a primary site of PTHrP production and release. Oocytes from all follicular stages stained strongly for PTHrP polypeptides and PTHrP enhanced development to the blastocyst stage in vitro...
  15. doi request reprint Bovine oocytes and early embryos express Staufen and ELAVL RNA-binding proteins
    M D Calder
    Department of Physiology and Pharmacology, University of Western Ontario, Children s Health Research Institute LHRI, 5th Floor Victoria Research Laboratories, 800 Commissioners Road, London, Ontario, Canada N6A 4G5
    Zygote 16:161-8. 2008
    ..The findings provide a foundation for investigating the role of RBPs during mammalian oocyte maturation and early embryogenesis...
  16. ncbi request reprint Transient expression of a translation initiation factor is conservatively associated with embryonic gene activation in murine and bovine embryos
    P A De Sousa
    Departments of Obstetrics and Gynaecology and Physiology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biol Reprod 59:969-77. 1998
    ..Our results suggest that transient expression of eIF-1A in the mouse and cow is a conserved pattern of gene expression associated with EGA in mammals...
  17. ncbi request reprint Effect of estrogen-treated porcine ampulla oviductal epithelial cells on early embryonic development in vitro and characterization of their protein synthetic activity
    P Xia
    Department of Obstetrics and Gynaecology, University of Western Ontario, London, Canada
    Anim Reprod Sci 45:217-29. 1996
    ....
  18. ncbi request reprint UPR-independent dithiothreitol stress-induced genes in Aspergillus niger
    D A MacKenzie
    Department of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
    Mol Genet Genomics 274:410-8. 2005
    ..We conclude that DTT alone does not provide for specific induction of UPR genes and that other stress conditions must also be examined...
  19. ncbi request reprint Deletion of the Na/K-ATPase alpha1-subunit gene (Atp1a1) does not prevent cavitation of the preimplantation mouse embryo
    L C Barcroft
    Department Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, OH 45267 0524, USA
    Mech Dev 121:417-26. 2004
    ....
  20. ncbi request reprint mRNAs encoding aquaporins are present during murine preimplantation development
    H Offenberg
    Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
    Mol Reprod Dev 57:323-30. 2000
    ..These findings predict that AQPs may function as conduits for trophectoderm fluid transport during blastocyst formation...
  21. ncbi request reprint Expression of genes encoding antioxidant enzymes in preimplantation mouse and cow embryos and primary bovine oviduct cultures employed for embryo coculture
    M B Harvey
    Department of Medical Biochemistry, University of Calgary, AB, Canada
    Biol Reprod 53:532-40. 1995
    ..These experiments suggest that differences in gene expression may contribute to the variation in the ability of embryos to develop in vitro with respect to levels of oxygen and dependence on coculture...