D Robert Sutherland

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. doi request reprint Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers
    D Robert Sutherland
    Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
    Cytotherapy 11:595-605. 2009
  2. doi request reprint Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria clones by flow cytometry
    D Robert Sutherland
    Laboratory Medicine Program, Toronto General Hospital, Toronto, Ontario, Canada
    Cytometry B Clin Cytom 82:195-208. 2012
  3. ncbi request reprint Quantification of CD34 epitopes in AML
    Robert Sutherland
    Department of Medical Oncology and Hematology, The University Health Network, Toronto, Ontario, Canada
    Haematologica 88:ELT22; author reply ELT23. 2003
  4. doi request reprint Use of a FLAER-based WBC assay in the primary screening of PNH clones
    D Robert Sutherland
    Laboratory Medicine Program, Toronto General Hospital 11E 416, Toronto, Ontario M5G 2C4, Canada
    Am J Clin Pathol 132:564-72. 2009
  5. ncbi request reprint Diagnosing PNH with FLAER and multiparameter flow cytometry
    D Robert Sutherland
    Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
    Cytometry B Clin Cytom 72:167-77. 2007
  6. ncbi request reprint Clonality analysis of cell lineages in acute myeloid leukemia with inversion 16
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, 610 University Avenue, 4 320 Toronto, ON, M5G 2M9, Canada
    Cancer Genet Cytogenet 156:175-8. 2005
  7. ncbi request reprint Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts
    Michael Keeney
    The London Health Sciences Centre, London, Ontario, Canada
    Cytometry A 58:72-5. 2004
  8. ncbi request reprint Identification of cell lineages involved by t(15;17) in acute promyelocytic leukemia by combined fluorescence activated cell sorting and FISH
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 158:43-8. 2005
  9. doi request reprint Enumeration of CD34+ hematopoietic stem and progenitor cells
    D Robert Sutherland
    The University Health Network, Toronto, Ontario, Canada
    Curr Protoc Cytom . 2003
  10. ncbi request reprint A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens
    Andre C Schuh
    Institute of Medical Science and the Department of Medicine, University of Toronto, ON, Canada
    Blood 99:1692-8. 2002

Collaborators

Detail Information

Publications12

  1. doi request reprint Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers
    D Robert Sutherland
    Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
    Cytotherapy 11:595-605. 2009
    ..5x10(6) viable CD34(+) cells (measured pre-cryopreservation)/kg patient weight will reliably permit engraftment of the hematopoietic system (as measured by the time to 20000 platelets/microL) by day 12-14 post-infusion...
  2. doi request reprint Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria clones by flow cytometry
    D Robert Sutherland
    Laboratory Medicine Program, Toronto General Hospital, Toronto, Ontario, Canada
    Cytometry B Clin Cytom 82:195-208. 2012
    ....
  3. ncbi request reprint Quantification of CD34 epitopes in AML
    Robert Sutherland
    Department of Medical Oncology and Hematology, The University Health Network, Toronto, Ontario, Canada
    Haematologica 88:ELT22; author reply ELT23. 2003
  4. doi request reprint Use of a FLAER-based WBC assay in the primary screening of PNH clones
    D Robert Sutherland
    Laboratory Medicine Program, Toronto General Hospital 11E 416, Toronto, Ontario M5G 2C4, Canada
    Am J Clin Pathol 132:564-72. 2009
    ..2%), and always with a smaller clone size. The FLAER assay on WBCs is a more sensitive and robust primary screening assay for detecting PNH clones in clinical samples...
  5. ncbi request reprint Diagnosing PNH with FLAER and multiparameter flow cytometry
    D Robert Sutherland
    Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
    Cytometry B Clin Cytom 72:167-77. 2007
    ..Our goals were to develop a FLAER-based assay to diagnose and monitor patients with PNH and to improve detection of minor populations of PNH clones in other hematologic disorders...
  6. ncbi request reprint Clonality analysis of cell lineages in acute myeloid leukemia with inversion 16
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, 610 University Avenue, 4 320 Toronto, ON, M5G 2M9, Canada
    Cancer Genet Cytogenet 156:175-8. 2005
    ..The current study provides a direct evidence for the clonal involvement of myeloid lineage cells and B-lymphocytes and suggests that T-cells are not part of the malignant clone in this disease...
  7. ncbi request reprint Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts
    Michael Keeney
    The London Health Sciences Centre, London, Ontario, Canada
    Cytometry A 58:72-5. 2004
  8. ncbi request reprint Identification of cell lineages involved by t(15;17) in acute promyelocytic leukemia by combined fluorescence activated cell sorting and FISH
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 158:43-8. 2005
    ..These results suggest that lymphocytes and erythroblasts are not clonally involved in APL, and that malignant transformation in some cases of APL may occur at the level of CD34+ cells...
  9. doi request reprint Enumeration of CD34+ hematopoietic stem and progenitor cells
    D Robert Sutherland
    The University Health Network, Toronto, Ontario, Canada
    Curr Protoc Cytom . 2003
    ..All the possible problems are discussed and examples provided, allowing the clinical hematology lab to be certain that analysis of CD34(+) cells is performed in an appropriate manner...
  10. ncbi request reprint A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens
    Andre C Schuh
    Institute of Medical Science and the Department of Medicine, University of Toronto, ON, Canada
    Blood 99:1692-8. 2002
    ..We conclude that the Gov alleles are defined by a 2108 A>C SNP that results in a Tyr703Ser substitution of CD109 and that genotyping studies are more accurate for Gov alloantigen determination than are conventional serologic methods...
  11. ncbi request reprint Interphase cytogenetic analysis of clonality in peripheral blood cells from a patient with Down syndrome and acute megakaryoblastic leukemia
    Hong Chang
    Princess Margaret Hospital, University Health Network, University of Toronto, 4 320 Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 148:141-4. 2004
    ..The transformation occurs in progenitor cells with limited myeloid differentiation and without involvement of lymphoid lineage cells...
  12. ncbi request reprint Early hematopoietic reconstitution after clinical stem cell transplantation: evidence for stochastic stem cell behavior and limited acceleration in telomere loss
    Ian Thornley
    Division of Hematology Oncology, The Hospital for Sick Children, University Health Network, University of Toronto, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    Blood 99:2387-96. 2002
    ....