Yu Sun

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. ncbi request reprint In situ mechanical characterization of mouse oocytes using a cell holding device
    Xinyu Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, CanadaM5S 3G8
    Lab Chip 10:2154-61. 2010
  2. pmc Single cell deposition and patterning with a robotic system
    Zhe Lu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 5:e13542. 2010
  3. pmc Automated microinjection of recombinant BCL-X into mouse zygotes enhances embryo development
    Xinyu Liu
    Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 6:e21687. 2011
  4. ncbi request reprint Characterization of red blood cell deformability change during blood storage
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Lab Chip 14:577-83. 2014
  5. doi request reprint MEMS capacitive force sensors for cellular and flight biomechanics
    Yu Sun
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto M5S 3G8, Canada
    Biomed Mater 2:S16-22. 2007
  6. ncbi request reprint Autofocusing in computer microscopy: selecting the optimal focus algorithm
    Yu Sun
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Canada
    Microsc Res Tech 65:139-49. 2004
  7. doi request reprint Controlled aspiration and positioning of biological cells in a micropipette
    Xuping Zhang
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, ON, Canada
    IEEE Trans Biomed Eng 59:1032-40. 2012
  8. doi request reprint Automated micropipette aspiration of single cells
    Ehsan Shojaei-Baghini
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Ann Biomed Eng 41:1208-16. 2013
  9. doi request reprint In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats
    Michael K L Chu
    Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Lab Chip 12:2533-9. 2012
  10. doi request reprint Three-dimensional rotation of mouse embryos
    Clement Leung
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, ON, Canada
    IEEE Trans Biomed Eng 59:1049-56. 2012

Collaborators

Detail Information

Publications47

  1. ncbi request reprint In situ mechanical characterization of mouse oocytes using a cell holding device
    Xinyu Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, CanadaM5S 3G8
    Lab Chip 10:2154-61. 2010
    ..Biomembrane and cytoskeleton structures of the healthy and defective oocytes are also investigated in an attempt to correlate the measured subtle mechanical difference to cellular structure changes...
  2. pmc Single cell deposition and patterning with a robotic system
    Zhe Lu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 5:e13542. 2010
    ....
  3. pmc Automated microinjection of recombinant BCL-X into mouse zygotes enhances embryo development
    Xinyu Liu
    Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 6:e21687. 2011
    ..This approach may lead to a possible treatment option for patients with repeated in vitro fertilization (IVF) failure due to poor embryo quality...
  4. ncbi request reprint Characterization of red blood cell deformability change during blood storage
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Lab Chip 14:577-83. 2014
    ..However, significant differences exist in time constants and circularity distribution widths, which can be used to quantify stored RBC quality or age. ..
  5. doi request reprint MEMS capacitive force sensors for cellular and flight biomechanics
    Yu Sun
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto M5S 3G8, Canada
    Biomed Mater 2:S16-22. 2007
    ..This paper describes our research in the development of MEMS capacitive force sensors that have already demonstrated their effectiveness in the areas of cell mechanics and Drosophila flight dynamics studies...
  6. ncbi request reprint Autofocusing in computer microscopy: selecting the optimal focus algorithm
    Yu Sun
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Canada
    Microsc Res Tech 65:139-49. 2004
    ..Image preprocessing was also conducted to extensively reveal the performance and robustness of the focus algorithms. The presented guidelines allow for the selection of the optimal focus algorithm for different microscopy applications...
  7. doi request reprint Controlled aspiration and positioning of biological cells in a micropipette
    Xuping Zhang
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, ON, Canada
    IEEE Trans Biomed Eng 59:1032-40. 2012
    ....
  8. doi request reprint Automated micropipette aspiration of single cells
    Ehsan Shojaei-Baghini
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Ann Biomed Eng 41:1208-16. 2013
    ..Experiments on both solid-like and liquid-like cells demonstrated that the system is capable of efficiently performing single-cell micropipette aspiration and has low operator skill requirements...
  9. doi request reprint In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats
    Michael K L Chu
    Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Lab Chip 12:2533-9. 2012
    ..The in vitro and in vivo testing results demonstrated the efficacy of closed-loop biosensing and rapid response of the 'smart' insulin delivery devices...
  10. doi request reprint Three-dimensional rotation of mouse embryos
    Clement Leung
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, ON, Canada
    IEEE Trans Biomed Eng 59:1049-56. 2012
    ..Experimental results demonstrated the system's capability for polar-body orientation with a high success rate of 90%, an accuracy of 1.9 °, and an average speed of 22.8 s/cell (versus averagely 40 s/cell in manual operation)...
  11. doi request reprint A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial arrays
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Biomaterials 31:577-84. 2010
    ..This complex mechanical interaction between cells and biomaterials further emphasizes the need for high-throughput approaches to conduct mechanically active experiments in three-dimensional culture...
  12. doi request reprint Robotic ICSI (intracytoplasmic sperm injection)
    Zhe Lu
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto, ON M5S 3G8 Canada
    IEEE Trans Biomed Eng 58:2102-8. 2011
    ..Using the hamster oocyte-human sperm model in preliminary trials, the robotic system demonstrated a high success rate of 90.0% and survival rate of 90.7% (n=120)...
  13. ncbi request reprint Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformation
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 10:227-34. 2010
    ..More generally, this versatile platform has broad applicability in the fields of mechanobiology, tissue engineering and pathobiology...
  14. doi request reprint Classification of cell types using a microfluidic device for mechanical and electrical measurement on single cells
    Jian Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada
    Lab Chip 11:3174-81. 2011
    ..These preliminary results suggest that biomechanical and bioelectrical parameters, when used in combination, could provide a higher cell classification success rate than using electrical or mechanical parameter alone...
  15. ncbi request reprint Microfluidic characterization of specific membrane capacitance and cytoplasm conductivity of single cells
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Biosens Bioelectron 42:496-502. 2013
    ..12 S/m) cells. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results since these parameters contain information additional to cell size...
  16. doi request reprint Mechanical characterization of polymeric microcapsules using a force-feedback MEMS microgripper
    Keekyoung Kim
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, ON, Canada, M5S 3G8
    Conf Proc IEEE Eng Med Biol Soc 2008:1845-8. 2008
    ....
  17. doi request reprint Microdevice array-based identification of distinct mechanobiological response profiles in layer-specific valve interstitial cells
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Integr Biol (Camb) 5:673-80. 2013
    ....
  18. doi request reprint Investigation of mechanical properties of soft hydrogel microcapsules in relation to protein delivery using a MEMS force sensor
    Keekyoung Kim
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada
    J Biomed Mater Res A 92:103-13. 2010
    ..4 phosphate-buffered saline (PBS). Protein release rates were also determined in DDI water and PBS. Finally, protein release rates were correlated with mechanical properties of the microcapsules...
  19. doi request reprint High-throughput biophysical measurement of human red blood cells
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada
    Lab Chip 12:2560-7. 2012
    ..In comparison with previously reported microfluidic devices for single RBC biophysical measurement, this system has a higher throughput, higher signal to noise ratio, and the capability of performing multi-parameter measurements...
  20. doi request reprint Electrical measurement of red blood cell deformability on a microfluidic device
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Lab Chip 13:3275-83. 2013
    ..The experimental results proved the capability of the system for distinguishing different RBC populations based on their deformability with a throughput of ~10 cells s(-1)...
  21. doi request reprint Automated sperm immobilization for intracytoplasmic sperm injection
    Clement Leung
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto M5S 3G8, Canada
    IEEE Trans Biomed Eng 58:935-42. 2011
    ..95 μm; 2) a sperm tail visual tracking success rate of 96%; 3) a sperm immobilization success rate of 88.2% (based on 1000 trials); and 4) a speed of 6-7 s per successful immobilization...
  22. doi request reprint Biophysical characterization of bladder cancer cells with different metastatic potential
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, M5S 3G8, Canada
    Cell Biochem Biophys 68:241-6. 2014
    ..Quantitative differences in SMC and Young's modulus values of the high-grade and low-grade UCC cells are, for the first time, reported. ..
  23. ncbi request reprint Automated nanomanipulation for nanodevice construction
    Yan Liang Zhang
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Nanotechnology 23:065304. 2012
    ..This technology represents a seamless integration of wafer-scale microfabrication and automated nanorobotic manipulation for producing nano-FET sensors with consistent response across devices...
  24. doi request reprint Recent advances in microfluidic techniques for single-cell biophysical characterization
    Yi Zheng
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    Lab Chip 13:2464-83. 2013
    ..Different techniques are compared. The technical details, advantages, and limitations of various microfluidic devices are discussed...
  25. ncbi request reprint Integrating polyurethane culture substrates into poly(dimethylsiloxane) microdevices
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Biomaterials 30:5241-50. 2009
    ..More generally, this technique can improve the impact and applicability of microdevice-based research by facilitating the use of alternate, relevant biomaterials while maintaining the advantages of using PDMS for microdevice fabrication...
  26. pmc A fully automated robotic system for microinjection of zebrafish embryos
    WenHui Wang
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
    PLoS ONE 2:e862. 2007
    ....
  27. ncbi request reprint In situ mechanical characterization of the cell nucleus by atomic force microscopy
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada M5S 3G8
    ACS Nano 8:3821-8. 2014
    ..This technique has potential to become a reliable quantitative measurement tool for intracellular mechanics studies. ..
  28. doi request reprint Elastic and viscoelastic characterization of microcapsules for drug delivery using a force-feedback MEMS microgripper
    Keekyoung Kim
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto, Ontario M5S3G8, Canada
    Biomed Microdevices 11:421-7. 2009
    ....
  29. ncbi request reprint Miniaturized platform with on-chip strain sensors for compression testing of arrayed materials
    Luke Macqueen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 12:4178-84. 2012
    ..The miniaturized platform can significantly increase mechanical testing efficiency, particularly when testing of iterative sample formulations is required...
  30. doi request reprint Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection
    Xinyu Liu
    Advanced Micro and Nanosystems Laboratory, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada
    Biomed Microdevices 11:1169-74. 2009
    ..Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development...
  31. pmc Mesenchymal stem cell mechanobiology and emerging experimental platforms
    Luke Macqueen
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    J R Soc Interface 10:20130179. 2013
    ..Our main goals are to provide engineers and microtechnology developers with an up-to-date description of MSC mechanobiology that is relevant to the design of experimental platforms and to introduce biologists to these emerging platforms...
  32. doi request reprint Determination of local and global elastic moduli of valve interstitial cells cultured on soft substrates
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
    J Biomech 46:1967-71. 2013
    ..They also provide new physiologically-relevant data on VIC mechanical properties and their correlation with substrate stiffness-dependent cytoskeletal changes, with relevance to heart valve mechanobiology and disease. ..
  33. doi request reprint Quantitative analysis of locomotive behavior of human sperm head and tail
    Jun Liu
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, ON, Canada
    IEEE Trans Biomed Eng 60:390-6. 2013
    ....
  34. doi request reprint Effect of oscillating fluid flow stimulation on osteocyte mRNA expression
    Jason Li
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    J Biomech 45:247-51. 2012
    ..These findings suggest that dynamic fluid flow with higher peak shear stress amplitudes, faster oscillating frequencies, and longer loading durations provide the best conditions for promoting bone formation...
  35. doi request reprint (Micro)managing the mechanical microenvironment
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Integr Biol (Camb) 3:959-71. 2011
    ....
  36. doi request reprint An automated microfluidic sample preparation system for laser scanning cytometry
    Eric Wu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Biomed Microdevices 13:393-401. 2011
    ..This prototype system reduces 36 pipetting steps to 1, reduces the amount of cell sample from 180 μL to 56 μL, and shortens the time used by technicians...
  37. ncbi request reprint High-throughput measurement of gap junctional intercellular communication
    Jun Liu
    Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Ontario, Canada and
    Am J Physiol Heart Circ Physiol 306:H1708-13. 2014
    ..This robotic microinjection dye transfer technique permits rapid assessment of gap junction function in confluent cell cultures...
  38. doi request reprint A fast and simple method to fabricate circular microchannels in polydimethylsiloxane (PDMS)
    Mohamed Abdelgawad
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Lab Chip 11:545-51. 2011
    ..A comparison between the performance of square and circular microchannels in trapping SiHa cells (cervical cancer cell line) is shown...
  39. doi request reprint A monolithic polymeric microdevice for pH-responsive drug delivery
    Jian Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada
    Biomed Microdevices 11:1251-7. 2009
    ..4 to 4. This device concept can serve as a platform technology for intelligent drug delivery in response to various in vivo environmental signals...
  40. doi request reprint Effect of nanowire number, diameter, and doping density on nano-FET biosensor sensitivity
    Jason Li
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto ON M5S 3G8, Canada
    ACS Nano 5:6661-8. 2011
    ..These results provide insight into the importance of controlling nanowire properties for maximizing sensitivity and minimizing performance variation across devices when designing and manufacturing nano-FET biosensors...
  41. pmc Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle
    Yun Xiao
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, 164 College St, Rm 407, Toronto, ON M5S 3G9, Canada
    Lab Chip 14:869-82. 2014
    ..This microfabricated platform provides a unique opportunity to assess pharmacological effects on cardiac tissue in vitro by perfusion in a cardiac bundle model, which could provide improved physiological relevance. ..
  42. ncbi request reprint Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system
    Deok Ho Kim
    Microsystems Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130 650, Republic of Korea
    J Biomech 38:1359-3. 2005
    ..The results mechanically quantitate "chorion softening" in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development...
  43. ncbi request reprint Mechanical property characterization of mouse zona pellucida
    Yu Sun
    Swiss Federal Institute of Technology ETH Zurich, CH 8092 Zurich, Switzerland
    IEEE Trans Nanobioscience 2:279-86. 2003
    ..7 times those for oocyte ZP. The technique and model presented in this paper can be applied to investigations into the mechanical properties of other biomembranes, such as the plasma membrane of oocytes or other cell types...
  44. ncbi request reprint Prediction of the orientations of adsorbed protein using an empirical energy function with implicit solvation
    Yu Sun
    Department of Bioengineering and Center for Advanced Engineering Fibers and Films CAEFF, Clemson University, Clemson, South Carolina 29634, USA
    Langmuir 21:5616-26. 2005
    ....
  45. ncbi request reprint The discovery of transcriptional modules by a two-stage matrix decomposition approach
    Huai Li
    Bioinformatics Unit, Branch of Research Resources, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Bioinformatics 23:473-9. 2007
    ..Unraveling such transcriptional modules is important for understanding biological network, deciphering regulatory mechanisms and identifying biomarkers...
  46. ncbi request reprint Mechanical analysis of chorion softening in prehatching stages of zebrafish embryos
    Deok Ho Kim
    Microsystem Research Center, Korea Institute of Science and Technology, Seoul 138 791, Korea
    IEEE Trans Nanobioscience 5:89-94. 2006
    ..This similarity suggests that "chorion softening" may be induced by the release of protease from the embryos, and the chemical nature of the process involves proteolytic fragmentation of the ZP2 protein...
  47. ncbi request reprint The effect of 10-hydroxycamptothecine in preventing fibroblast proliferation and epidural scar adhesion after laminectomy in rats
    Yu Sun
    Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road 300, Nanjing, Jiangsu Province, China
    Eur J Pharmacol 593:44-8. 2008
    ..In conclusion, topical application of 0.1 mg/ml HCPT could effectively prevent fibroblast proliferation and reduce epidural adhesion after laminectomy in rats...