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Genomes and Genes | Lawrence L SprietSummaryAffiliation: University of Guelph Country: Canada Publications
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Regulation of pyruvate dehydrogenase (PDH) activity in human skeletal muscle during exerciseLawrence L Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
Exerc Sport Sci Rev 30:91-5. 2002..During exercise, pyruvate dehydrogenase activation in human skeletal muscle is proportional to the relative aerobic power output (percent VO2max) and is regulated by increases in Ca2+, free ADP, and pyruvate concentrations...
Effects of plasma adrenaline on hormone-sensitive lipase at rest and during moderate exercise in human skeletal muscleMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Physiol 550:325-32. 2003..The results also demonstrate that increased ERK 1/2 phosphorylation coincides with elevated HSL activity, indicating that ERK 1/2 may mediate the contraction-induced increase in HSL activity early in exercise...- The effects of training in hyperoxia vs. normoxia on skeletal muscle enzyme activities and exercise performanceChristopher G R Perry
Dept of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Appl Physiol 102:1022-7. 2007.... - Regulation of substrate use during the marathonLawrence L Spriet
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Sports Med 37:332-6. 2007..However, they compete at such high running speeds, that CHO oxidation (also highly adapted) may be the exclusive source of energy while racing. Further work with elite athletes is needed to examine this possibility... - Time course of insulin sensitivity and skeletal muscle glycogen synthase activity after a single bout of exercise in horsesShannon E Pratt
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada
J Appl Physiol 103:1063-9. 2007.... - Nutritional strategies to influence adaptations to trainingLawrence L Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Sports Sci 22:127-41. 2004..Research in these areas in the coming years will lead to strategies designed to improve the adaptive response to training... 
Legal pre-event nutritional supplements to assist energy metabolismLawrence L Spriet
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Essays Biochem 44:27-43. 2008..However, other studies have reported no benefit and the incidence of negative side effects is high...- Regulation of skeletal muscle fat oxidation during exercise in humansLawrence L Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Med Sci Sports Exerc 34:1477-84. 2002..VO(2max)). Although it is expected that the signals that activate carbohydrate metabolism during exercise (Ca and free ADP, AMP, and P(i)) would also play a role in fat metabolism, this has not been demonstrated to date... - Adrenergic regulation of HSL serine phosphorylation and activity in human skeletal muscle during the onset of exerciseJason L Talanian
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Am J Physiol Regul Integr Comp Physiol 291:R1094-9. 2006..This adrenergic stimulation contributes to the increase in HSL activity that occurs in human skeletal muscle in the first minute of exercise at 65% and 90% VO2 peak... - Exercise training increases sarcolemmal and mitochondrial fatty acid transport proteins in human skeletal muscleJason L Talanian
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Am J Physiol Endocrinol Metab 299:E180-8. 2010..These results suggest that increases in skeletal muscle fatty acid oxidation following training are related in part to changes in fatty acid transport protein content and localization... - High-intensity aerobic interval training increases fat and carbohydrate metabolic capacities in human skeletal muscleChristopher G R Perry
Department of Human Health and Nutritional Sciences, University of Guelph, ON N1G 2W1, Canada
Appl Physiol Nutr Metab 33:1112-23. 2008..week-1) is a powerful method to increase whole-body and skeletal muscle capacities to oxidize fat and carbohydrate in previously untrained individuals... - Mitochondrial long chain fatty acid oxidation, fatty acid translocase/CD36 content and carnitine palmitoyltransferase I activity in human skeletal muscle during aerobic exerciseGraham P Holloway
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada
J Physiol 571:201-10. 2006..We further suggest that decreased CPTI M-CoA sensitivity and increased mitochondrial FAT/CD36 protein are both important for increasing whole body fatty acid oxidation during prolonged exercise... - Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscleGraham P Holloway
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada, N1G 2W1
J Physiol 582:393-405. 2007.... - Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese womenGraham P Holloway
Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd, Guelph, Ontario, Canada N1G2W1
Am J Physiol Endocrinol Metab 292:E1782-9. 2007..In addition, it appears that mitochondrial FAT/CD36 may be involved in regulating fatty acid oxidation in human skeletal muscle... - Fluid and electrolyte supplementation after prolonged moderate-intensity exercise enhances muscle glycogen resynthesis in Standardbred horsesAmanda P Waller
Dept of Human Health and Nutritional Sciences, Univ of Guelph, Guelph, ON, Canada N1G 2W1
J Appl Physiol 106:91-100. 2009..It is speculated that postexercise dehydration may be one key contributor to the slow muscle glycogen replenishment in horses... - Decreased PDH activation and glycogenolysis during exercise following fat adaptation with carbohydrate restorationTrent Stellingwerff
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
Am J Physiol Endocrinol Metab 290:E380-8. 2006.... - Effects of hyperoxia on skeletal muscle carbohydrate metabolism during transient and steady-state exerciseTrent Stellingwerff
Dept of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Appl Physiol 98:250-6. 2005..The mechanism responsible for the decreased muscle glycogenolysis during hyperoxia in the present study is not clear... - PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta proteinGraham P Holloway
Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
Am J Physiol Endocrinol Metab 294:E1060-9. 2008..Also, it does not appear that alterations in the proteins associated with mitochondrial network formation and degradation can account for the observed decrease in mitochondrial content... - Hormone-sensitive lipase activity and fatty acyl-CoA content in human skeletal muscle during prolonged exerciseMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
J Appl Physiol 95:314-21. 2003..The accumulation of LCFA CoA in the 2nd h of exercise may also have reduced the flux through HSL and accounted for the reduction in IMTG utilization previously observed late in prolonged exercise... - Skeletal muscle fat and carbohydrate metabolism during recovery from glycogen-depleting exercise in humansNicholas E Kimber
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Physiol 548:919-27. 2003..Plasma fatty acids, very low density lipoprotein triacylglycerols, as well as intramuscular acetylcarnitine stores are likely to be important fuel sources for aerobic energy, particularly during the first few hours of recovery... - Regulation of skeletal muscle mitochondrial fatty acid metabolism in lean and obese individualsGraham P Holloway
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
Am J Clin Nutr 89:455S-62S. 2009..These findings imply that obese individuals would benefit from interventions that increase the skeletal muscle mitochondrial content and the potential for oxidizing FAs... - Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exerciseMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1
J Appl Physiol 97:1261-7. 2004..The increased PDK activity was independent of changes in intra-mitochondrial effectors, and PDK-2 and PDK-4 protein content, suggesting that it was caused by a change in the specific activity of the existing kinases... - Oral acetate supplementation after prolonged moderate intensity exercise enhances early muscle glycogen resynthesis in horsesAmanda P Waller
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G2W1
Exp Physiol 94:888-98. 2009..It is concluded that oral acetate could be an alternative energy source in the horse... - Effects of dehydration during cycling on skeletal muscle metabolism in femalesHeather M Logan-Sprenger
Department of Human Health and Nutritional Sciences, University of Guelph, Ontario, Canada
Med Sci Sports Exerc 44:1949-57. 2012..This study investigated the effects of progressive dehydration on the time course of changes to whole body substrate oxidation and skeletal muscle metabolism during 120 min of cycling in hydrated females... - Hyperoxia decreases muscle glycogenolysis, lactate production, and lactate efflux during steady-state exerciseTrent Stellingwerff
Dept. of Human Health and Nutritional Sciences, Univ. of Guelph, Guelph, ON, N1G 2W1, Canada
Am J Physiol Endocrinol Metab 290:E1180-90. 2006..The tighter metabolic match between pyruvate production and oxidation resulted in a decrease in total lactate production and efflux over 40 min of exercise during hyperoxia... - Enzymatic regulation of glucose disposal in human skeletal muscle after a high-fat, low-carbohydrate dietTanya L Pehleman
Dept. of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Appl Physiol 98:100-7. 2005..The rapid increase in PDK activity during the HF/LC diet appeared to account for the reduced potential for oxidative carbohydrate disposal... - Intramuscular triacylglycerol, glycogen and acetyl group metabolism during 4 h of moderate exercise in manMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario N1G 2W1
J Physiol 541:969-78. 2002..Although the factor underlying the PDHa decrease was not apparent, reduced pyruvate provision secondary to diminished glycolytic flux is the most likely mechanism... - Rosiglitazone increases fatty acid oxidation and fatty acid translocase (FAT/CD36) but not carnitine palmitoyltransferase I in rat muscle mitochondriaCarley R Benton
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Physiol 586:1755-66. 2008..Moreover, these studies identify for the first time a mechanism by which rosiglitazone stimulates fatty acid oxidation in skeletal muscle, namely the chronic, subcellular relocation of FAT/CD36 to mitochondria... - Effects of reduced free fatty acid availability on hormone-sensitive lipase activity in human skeletal muscle during aerobic exerciseMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, ON, Canada N1G 2W1
J Appl Physiol 97:1938-45. 2004..In conclusion, reduced plasma FFA availability accompanied by increased epinephrine concentration did not further activate HSL beyond exercise alone... - The effect of acute taurine ingestion on endurance performance and metabolism in well-trained cyclistsJane A Rutherford
Dept of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Int J Sport Nutr Exerc Metab 20:322-9. 2010..The acute ingestion of 1.66 g of T before exercise did not enhance TT performance but did result in a small but significant increase in fat oxidation during submaximal cycling in endurance-trained cyclists... - Negligible direct lactate oxidation in subsarcolemmal and intermyofibrillar mitochondria obtained from red and white rat skeletal muscleYuko Yoshida
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Physiol 582:1317-35. 2007..e. an intracellular lactate shuttle) does not occur within the matrix in either IMF or SS mitochondria obtained from red or white rat skeletal muscle, because of the very limited quantity of LDH within mitochondria... - On-ice sweat rate, voluntary fluid intake, and sodium balance during practice in male junior ice hockey players drinking water or a carbohydrate-electrolyte solutionMatthew S Palmer
Department of Human Health and Nutritional Sciences, University of Guelph, ON N1G 2W1, Canada
Appl Physiol Nutr Metab 35:328-35. 2010..The CES provided some salt to offset the salt lost in sweat, and carbohydrate, which may help maintain physical and mental performance in the later stages of practice... - Two weeks of high-intensity aerobic interval training increases the capacity for fat oxidation during exercise in womenJason L Talanian
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
J Appl Physiol 102:1439-47. 2007..In summary, seven sessions of HIIT over 2 wk induced marked increases in whole body and skeletal muscle capacity for fatty acid oxidation during exercise in moderately active women... - Carbohydrate ingestion reduces skeletal muscle acetylcarnitine availability but has no effect on substrate phosphorylation at the onset of exercise in manMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario N1G 2W1, Canada
J Physiol 544:949-56. 2002..These data suggest that acetylcarnitine availability is unlikely to be the site of metabolic inertia during the transition from rest to steady-state moderate intensity exercise... - Effects of reduced free fatty acid availability on skeletal muscle PDH activation during aerobic exercise. Pyruvate dehydrogenaseTrent Stellingwerff
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Am J Physiol Endocrinol Metab 284:E589-96. 2003..The increased activation of PDH was not explained by changes in muscle pyruvate or the ATP/ADP ratio but may be related to a decrease in the NADH/NAD(+) ratio or an epinephrine-induced increase in calcium concentration... - Effects of dynamic exercise intensity on the activation of hormone-sensitive lipase in human skeletal muscleMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Physiol 547:301-8. 2003.... - Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fastingLawrence L Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Appl Physiol 96:2082-7. 2004..The changes in PDK-4 expression and PDH activity did not coincide with increases in the transcriptional activators PPAR-alpha and FKHR... - Estimated fluid and sodium balance and drink preferences in elite male junior players during an ice hockey gameHeather M Logan-Sprenger
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada
Appl Physiol Nutr Metab 36:145-52. 2011..Losing 2% BM has been associated with decreases in athletic performance... - Regulation and role of hormone-sensitive lipase activity in human skeletal muscleMatthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
Proc Nutr Soc 63:315-22. 2004..For example, it must be remembered that the actual flux (IMTG degradation) through HSL may be allosterically inhibited during prolonged exercise as a result of the accumulation of long-chain fatty acyl-CoA... - Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscleChristopher G R Perry
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
J Physiol 590:5475-86. 2012..This enhanced regulation of miCK-dependent phosphate shuttling may improve energy homeostasis through more efficient coupling of oxidative phosphorylation to perturbations in cellular energy charge during subsequent bouts of contraction... - Acute endurance exercise increases plasma membrane fatty acid transport proteins in rat and human skeletal muscleNicolette S Bradley
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Am J Physiol Endocrinol Metab 302:E183-9. 2012..This study is also the first to demonstrate that endurance cycling induces an increase in plasma membrane FAT/CD36 and FABPpm content in human skeletal muscle, which is predicted to increase fatty acid transport... - Effects of microhydrin supplementation on endurance performance and metabolism in well-trained cyclistsLee R Glazier
Dept of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Int J Sport Nutr Exerc Metab 14:560-73. 2004..05). The results indicate that oral Microhydrin supplementation does not enhance cycling time trial performance or alter metabolism during prolonged submaximal exercise in endurance-trained cyclists... - Skeletal muscle metabolism is unaffected by DCA infusion and hyperoxia after onset of intense aerobic exerciseIngrid Savasi
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Am J Physiol Endocrinol Metab 283:E108-15. 2002.... - Identification of fatty acid translocase on human skeletal muscle mitochondrial membranes: essential role in fatty acid oxidationVeronic Bezaire
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
Am J Physiol Endocrinol Metab 290:E509-15. 2006.... - Metabolic regulation of fat use during exercise and in recoveryLawrence L Spriet
Department of Human Health and Nutritional Science, University of Guelph, Guelph, ON, Canada
Nestle Nutr Inst Workshop Ser 69:39-53; discussion 53-8. 2011..Recent work using molecular and cellular compartmentalization techniques have advanced the knowledge in this area... - Intramuscular triacylglycerol utilization in human skeletal muscle during exercise: is there a controversy?Matthew J Watt
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Appl Physiol 93:1185-95. 2002..Therefore, it is our contention that the muscle biopsy, isotope tracer, and (1)H-MRS techniques report significant and energetically important oxidation of free fatty acids derived from IMTGs during prolonged moderate exercise... - Regulation of CPT I activity in intermyofibrillar and subsarcolemmal mitochondria from human and rat skeletal muscleVeronic Bezaire
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Am J Physiol Endocrinol Metab 286:E85-91. 2004..Exercise-induced increases in calcium and adenylate charge metabolites do not appear responsible for upregulating CPT I activity in human or rat skeletal muscle during moderate aerobic exercise... - AMP-activated protein kinase is required for exercise-induced peroxisome proliferator-activated receptor co-activator 1 translocation to subsarcolemmal mitochondria in skeletal muscleBrennan K Smith
Human Health and Nutritional Sciences, University of Guelph, 491 Gordon St, Guelph, ON, N1G 2W1, Canada
J Physiol 591:1551-61. 2013..These data suggest that exercise causes translocation of PGC-1α preferentially to SS mitochondria in an AMPK-dependent manner... - The acute effects of fluid intake on urine specific gravity and fluid retention in a mildly dehydrated stateHeather M Logan-Sprenger
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
J Strength Cond Res 27:1002-8. 2013..Following this practice will minimize the incidence of starting a practice or competition hypohydrated... - Conjugated linoleic acid improves insulin sensitivity in young, sedentary humansValerie Eyjolfson
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
Med Sci Sports Exerc 36:814-20. 2004..However, there is considerable individual variability in the response. Additional studies are required to identify underlying metabolic changes in human skeletal muscle... - Sweat rate, salt loss, and fluid intake during an intense on-ice practice in elite Canadian male junior hockey playersMatthew S Palmer
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
Appl Physiol Nutr Metab 33:263-71. 2008..However, 1/3 of players lost more than 1% body mass despite ready access to fluid and numerous drinking opportunities from the coaches... - Fasting activates the gene expression of UCP3 independent of genes necessary for lipid transport and oxidation in skeletal muscleRebecca J Tunstall
School of Health Sciences, Deakin University, Burwood, VIC 3125, Australia
Biochem Biophys Res Commun 294:301-8. 2002..This suggests a unique role for UCP3 in the regulation of FA homeostasis during fasting as adaptation to 40 h of fasting does not require alterations in the expression of other genes necessary for lipid metabolism... - Significant intramyocellular lipid use during prolonged cycling in endurance-trained males as assessed by three different methodologiesTrent Stellingwerff
Department of Movement Science, Maastricht University, Maastricht, The Netherlands
Am J Physiol Endocrinol Metab 292:E1715-23. 2007..In addition, this study shows differences in resting subcellular intramyocellular lipid deposit distribution and in the subsequent net use of these deposits during exercise... - Seven days of oral taurine supplementation does not increase muscle taurine content or alter substrate metabolism during prolonged exercise in humansStuart D R Galloway
Department of Sports Studies, University of Stirling, Stirling, United Kingdom
J Appl Physiol 105:643-51. 2008.... - Hormone-sensitive lipase activity and triacylglycerol hydrolysis are decreased in rat soleus muscle by cyclopiazonic acidMatthew J Watt
School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, Victoria 3083, Australia
Am J Physiol Endocrinol Metab 285:E412-9. 2003..It is possible that more powerful signals or a higher [Ca2+] may override the lipid-storage effect of the CPA-mediated effects during muscular contractions... - Reply to letter "Pyruvate metabolism in working human skeletal muscle" by Henderson et alTrent Stellingwerff
Am J Physiol Endocrinol Metab 292:E1238-9. 2007 - Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and femalesRobyn M Murphy
School of Health Sciences, Deakin University, Burwood, Australia
Mol Cell Biochem 244:151-7. 2003..8 +/- 2.2, females: 125.3 +/- 4.3 mmol.kg(-1) dry mass) were also unaffected (p > 0.05) by gender. These data demonstrate that gender does not influence skeletal muscle TCr content and CreaT gene expression in young human subjects...