Pamela A Sokol

Summary

Affiliation: University of Calgary
Country: Canada

Publications

  1. pmc Identification of a siderophore receptor required for ferric ornibactin uptake in Burkholderia cepacia
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 68:6554-60. 2000
  2. ncbi request reprint The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Microbiology 149:3649-58. 2003
  3. ncbi request reprint Communication systems in the genus Burkholderia: global regulators and targets for novel antipathogenic drugs
    Pamela A Sokol
    University of Calgary, Department of Microbiology and Infectious Diseases, Faculty of Medicine, 3330 Hospital Dr, NW Calgary, Alberta T2N 4N1, Canada
    Future Microbiol 2:555-63. 2007
  4. pmc Identification of potential CepR regulated genes using a cep box motif-based search of the Burkholderia cenocepacia genome
    Catherine E Chambers
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada
    BMC Microbiol 6:104. 2006
  5. ncbi request reprint Immunization with a Pseudomonas aeruginosa elastase peptide reduces severity of experimental lung infections due to P. aeruginosa Or Burkholderia cepacia
    P A Sokol
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    J Infect Dis 181:1682-92. 2000
  6. pmc Role of ornibactin biosynthesis in the virulence of Burkholderia cepacia: characterization of pvdA, the gene encoding L-ornithine N(5)-oxygenase
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1
    Infect Immun 67:4443-55. 1999
  7. pmc A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation
    Rebecca J Malott
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Bacteriol 191:2447-60. 2009
  8. pmc Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia
    Eoin P O'Grady
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    BMC Genomics 10:441. 2009
  9. pmc A LysR-type transcriptional regulator in Burkholderia cenocepacia influences colony morphology and virulence
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr, N W, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 76:38-47. 2008
  10. pmc Use of suppression-subtractive hybridization to identify genes in the Burkholderia cepacia complex that are unique to Burkholderia cenocepacia
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Dr N W, Calgary, Alberta, Canada
    J Bacteriol 187:5278-91. 2005

Collaborators

Detail Information

Publications45

  1. pmc Identification of a siderophore receptor required for ferric ornibactin uptake in Burkholderia cepacia
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 68:6554-60. 2000
    ..The orbA mutant was less virulent in a chronic respiratory infection model than the parent strain, indicating that ornibactin uptake and utilization are important in the pathogenesis of B. cepacia respiratory infections...
  2. ncbi request reprint The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Microbiology 149:3649-58. 2003
    ..cenocepacia infections. A zinc metalloprotease gene (zmpA) was shown to be regulated by CepR and may be one of the factors that accounts for the difference in virulence between the cepI mutant and the parent strain...
  3. ncbi request reprint Communication systems in the genus Burkholderia: global regulators and targets for novel antipathogenic drugs
    Pamela A Sokol
    University of Calgary, Department of Microbiology and Infectious Diseases, Faculty of Medicine, 3330 Hospital Dr, NW Calgary, Alberta T2N 4N1, Canada
    Future Microbiol 2:555-63. 2007
    ..QS systems have been demonstrated to be essential for full virulence in various infection models and, thus, these regulatory systems represent attractive targets for the development of novel therapeutics...
  4. pmc Identification of potential CepR regulated genes using a cep box motif-based search of the Burkholderia cenocepacia genome
    Catherine E Chambers
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada
    BMC Microbiol 6:104. 2006
    ..A bioinformatics approach was used to screen the B. cenocepacia J2315 genome for CepR binding site motifs...
  5. ncbi request reprint Immunization with a Pseudomonas aeruginosa elastase peptide reduces severity of experimental lung infections due to P. aeruginosa Or Burkholderia cepacia
    P A Sokol
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    J Infect Dis 181:1682-92. 2000
    ..These studies suggest that immunization with pep15 can reduce the severity of lung infections due to P. aeruginosa or B. cepacia...
  6. pmc Role of ornibactin biosynthesis in the virulence of Burkholderia cepacia: characterization of pvdA, the gene encoding L-ornithine N(5)-oxygenase
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1
    Infect Immun 67:4443-55. 1999
    ..The pvdA mutants were less virulent than the parent strain in chronic and acute models of respiratory infection. A functional pvdA gene appears to be required for effective colonization and persistence in B. cepacia lung infections...
  7. pmc A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation
    Rebecca J Malott
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Bacteriol 191:2447-60. 2009
    ..This study demonstrates that the orphan LuxR homolog CepR2 contributes to the quorum-sensing regulatory network in two distinct strains of B. cenocepacia...
  8. pmc Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia
    Eoin P O'Grady
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    BMC Genomics 10:441. 2009
    ..The B. cenocepacia quorum sensing network includes the CepIR and CciIR regulatory systems...
  9. pmc A LysR-type transcriptional regulator in Burkholderia cenocepacia influences colony morphology and virulence
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr, N W, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 76:38-47. 2008
    ..cenocepacia correlates with reduced biofilm formation and virulence, and we determined that BCAS0225 is one gene involved in the regulation of these phenotypes...
  10. pmc Use of suppression-subtractive hybridization to identify genes in the Burkholderia cepacia complex that are unique to Burkholderia cenocepacia
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Dr N W, Calgary, Alberta, Canada
    J Bacteriol 187:5278-91. 2005
    ..Other genes specific to B. cenocepacia and/or the ET12 lineage were shown to play a role in biofilm formation and swarming or swimming motility...
  11. pmc A unique regulator contributes to quorum sensing and virulence in Burkholderia cenocepacia
    Eoin P O'Grady
    Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    PLoS ONE 7:e37611. 2012
    ..Gene expression and phenotypic assays as well as vertebrate and invertebrate infection models showed that BCAM1871 significantly contributes to pathogenesis in B. cenocepacia...
  12. pmc Expression of the bviIR and cepIR quorum-sensing systems of Burkholderia vietnamiensis
    Rebecca J Malott
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr N W, Calgary, Alberta, Canada
    J Bacteriol 189:3006-16. 2007
    ..Lack of expression of G4 bviI in these three strains indicated that an additional regulatory element may be involved in the regulation of bviIR expression in certain strains of B. vietnamiensis...
  13. pmc Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence
    Sujatha Subramoni
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Infect Immun 79:2984-97. 2011
    ..Together, our data show that the ShvR-regulated genes BCAS0208 to BCAS0201 are required for the rough colony morphotype, biofilm and pellicle formation, and virulence in B. cenocepacia...
  14. doi request reprint Role of Burkholderia cenocepacia afcE and afcF genes in determining lipid-metabolism-associated phenotypes
    Sujatha Subramoni
    Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada
    Microbiology 159:603-14. 2013
    ..Taken together, these results indicate that afcE and afcF are involved in metabolic pathway(s) influencing lipid profiles and affect both cell surface and antifungal properties of B. cenocepacia...
  15. pmc Quorum-sensing mutations affect attachment and stability of Burkholderia cenocepacia biofilms
    Kerry L Tomlin
    Department of Biological Sciences, University of Calgary, 2500 University Drive N W, Calgary, Alberta, Canada T2N 1N4
    Appl Environ Microbiol 71:5208-18. 2005
    ....
  16. ncbi request reprint Interspecies communication between Burkholderia cepacia and Pseudomonas aeruginosa
    Shawn Lewenza
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, AB, Canada
    Can J Microbiol 48:707-16. 2002
    ..It is possible that a mixed bacterial population of B. cepacia and P. aeruginosa can coordinately regulate some of their virulence factors and influence the progression of lung disease due to infection with these organisms...
  17. pmc Comparative analysis of plant and animal models for characterization of Burkholderia cepacia virulence
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 71:5306-13. 2003
    ..Therefore, this alfalfa infection model may be a useful tool for assessing virulence of strains of the B. cepacia complex and identifying new virulence-associated genes...
  18. pmc Detection of N-acyl homoserine lactones using a traI-luxCDABE-based biosensor as a high-throughput screening tool
    Steve P Bernier
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada
    BMC Biotechnol 8:59. 2008
    ..Bacteria use N-acyl homoserine lactone (AHL) molecules to regulate the expression of genes in a density-dependent manner. Several biosensors have been developed and engineered to detect the presence of all types of AHLs...
  19. pmc Identification of genes regulated by the cepIR quorum-sensing system in Burkholderia cenocepacia by high-throughput screening of a random promoter library
    Benchamas Subsin
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 189:968-79. 2007
    ..This study extends our understanding of genes whose expression is influenced by cepIR and indicates the global regulatory effect of the cepIR system in B. cenocepacia...
  20. pmc Characterization of the cciIR quorum-sensing system in Burkholderia cenocepacia
    Rebecca J Malott
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr N W, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 73:4982-92. 2005
    ..The cciR mutant had less protease activity than the parent strain. The phenotypes of the cepI cciI and cepR cciR mutants were similar to cepI or cepR mutants, with less protease activity and swarming motility than the parent strain...
  21. pmc The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes
    Eoin P O'Grady
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    J Bacteriol 193:163-76. 2011
    ..In summary, ShvR was determined to have a significant influence on the expression of quorum-sensing, protease, lipase, type II secretion, and afc genes...
  22. pmc Importance of the ornibactin and pyochelin siderophore transport systems in Burkholderia cenocepacia lung infections
    M B Visser
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 72:2850-7. 2004
    ..These studies suggest that the ornibactin uptake system is the most important siderophore-mediated iron transport system in B. cenocepacia lung infections...
  23. ncbi request reprint Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients
    Catherine E Chambers
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alta, Canada T2N 4N1
    FEMS Microbiol Lett 244:297-304. 2005
    ..OHL and N-decanoyl-l-homoserine lactone were detected in nanomolar concentrations compared to picomolar amounts of the 3-oxo-derivatives of the AHLs identified...
  24. pmc Functional quorum sensing systems are maintained during chronic Burkholderia cepacia complex infections in patients with cystic fibrosis
    Suzanne A McKeon
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    J Infect Dis 203:383-92. 2011
    ..These data suggest that there is a selective advantage for strains with QS systems and that QS mutations do not occur at a high frequency in B. cepacia complex isolates...
  25. doi request reprint Burkholderia cenocepacia zinc metalloproteases influence resistance to antimicrobial peptides
    Cora Kooi
    Department of Microbiology, University of Calgary, Calgary AB T2N 4N1, Canada
    Microbiology 155:2818-25. 2009
    ..These studies suggest that ZmpA and ZmpB may influence the resistance of B. cenocepacia to host antimicrobial peptides as well as alter the host protease/anti-protease balance in chronic respiratory infections...
  26. ncbi request reprint An extracellular zinc metalloprotease gene of Burkholderia cepacia
    C R Corbett
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1
    Microbiology 149:2263-71. 2003
    ..cepacia strain Pc715j and a Pc715j zmpA mutant. The results indicate that this extracellular zinc metalloprotease may play a greater role in virulence in some strains of B. cepacia...
  27. pmc Identification and characterization of the tolQRA genes of Pseudomonas aeruginosa
    J J Dennis
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Alberta, Canada
    J Bacteriol 178:7059-68. 1996
    ..aeruginosa. Attempts to inactivate the chromosomal copy of the tolA or tolQ gene in the parent strain PAO proved to be unsuccessful, and we propose that inactivation of these genes in P. aeruginosa results in a lethal phenotype...
  28. pmc Distribution of quorum-sensing genes in the Burkholderia cepacia complex
    E Lutter
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 69:4661-6. 2001
    ..bviIR were amplified from B. vietnamiensis. All genomovars produced N-octanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone. B. vietnamiensis and B. cepacia genomovar VII produced additional N-acyl-L-homoserine lactones...
  29. pmc Quorum sensing in Burkholderia cepacia: identification of the LuxRI homologs CepRI
    S Lewenza
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 181:748-56. 1999
    ..K56-I2 hyperproduced ornibactin and did not produce protease. These data suggest both a positive and negative role for cepIR in the regulation of extracellular virulence factor production by B. cepacia...
  30. doi request reprint Quorum sensing systems influence Burkholderia cenocepacia virulence
    Sujatha Subramoni
    Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    Future Microbiol 7:1373-87. 2012
    ..QS inhibitors in combination with antibiotics can reduce biofilm formation and virulence in infection models...
  31. pmc Regulation of ornibactin biosynthesis and N-acyl-L-homoserine lactone production by CepR in Burkholderia cepacia
    S Lewenza
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 183:2212-8. 2001
    ..These results suggest that CepR is both a positive and negative transcriptional regulator and that CepR may influence the expression of ornibactin biosynthetic genes in addition to the expression of the cepIR quorum-sensing system...
  32. pmc RegA, iron, and growth phase regulate expression of the Pseudomonas aeruginosa tol-oprL gene cluster
    K Duan
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada
    J Bacteriol 182:2077-87. 2000
    ..RegA is a positive yet nonessential regulator of tol-oprL expression...
  33. doi request reprint The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling
    Daniel F Aubert
    Centre for Human Immunology, Department of Microbiology, University of Western Ontario, London, Ontario, Canada, N6A 5C1 Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada, T2N 4N1
    Environ Microbiol 15:372-85. 2013
    ..Together, our results strongly suggest that AtsR is a global virulence regulator in B.‚ÄÉcenocepacia...
  34. pmc Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment
    Eoin P O'Grady
    Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada
    Front Cell Infect Microbiol 1:15. 2011
    ..Genes identified in this study are potentially important for virulence during host-pathogen interactions and may be associated with survival and adaptation to the host environment during chronic lung infections...
  35. pmc Detection of bacterial virulence genes by subtractive hybridization: identification of capsular polysaccharide of Burkholderia pseudomallei as a major virulence determinant
    S L Reckseidler
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 69:34-44. 2001
    ..Thus, the combination of PCR-based subtractive hybridization, insertional inactivation, and animal virulence studies has facilitated the identification of an important virulence determinant in B. pseudomallei...
  36. pmc Distribution and expression of the ZmpA metalloprotease in the Burkholderia cepacia complex
    S Gingues
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada
    J Bacteriol 187:8247-55. 2005
    ..These studies suggest that although some strains of the Bcc contain the zmpA gene, multiple factors may influence its expression...
  37. pmc Functional analysis of the Burkholderia cenocepacia ZmpA metalloprotease
    C Kooi
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 187:4421-9. 2005
    ..cenocepacia ZmpA may cause direct tissue damage to the host or damage to host tissues through a modulation of the host's immune system...
  38. pmc Burkholderia cenocepacia ZmpB is a broad-specificity zinc metalloprotease involved in virulence
    C Kooi
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada, USA
    Infect Immun 74:4083-93. 2006
    ..cenocepacia virulence. Expression of zmpB was regulated by both the CepIR and CciIR quorum-sensing systems...
  39. ncbi request reprint Putative virulence factors are released in association with membrane vesicles from Burkholderia cepacia
    Nick D Allan
    Canadian Bacterial Disease Network National Centre of Excellence, Department of Microbiology, College of Biological Science, University of Guelph, ON
    Can J Microbiol 49:613-24. 2003
    ..This finding suggests that certain components are selected by B. cepacia during n-MV formation, and since some are putative virulence factors, this property could help deliver the factors to tissue, thereby aiding infection...
  40. pmc The Burkholderia cepacia epidemic strain marker is part of a novel genomic island encoding both virulence and metabolism-associated genes in Burkholderia cenocepacia
    Adam Baldwin
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, Wales, Canada TN2 4N1
    Infect Immun 72:1537-47. 2004
    ....
  41. pmc A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo
    Slade A Loutet
    Department of Microbiology and Immunology, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5C1
    J Bacteriol 188:2073-80. 2006
    ..Together, these data show that the B. cenocepacia LPS inner core oligosaccharide is needed for in vitro resistance to three structurally unrelated antimicrobial peptides and for in vivo survival in a rat model of chronic lung infection...
  42. doi request reprint Virulence determinants from a cystic fibrosis isolate of Pseudomonas aeruginosa include isocitrate lyase
    Tamishia L Lindsey
    Department of Biological Sciences, Auburn University, AL 36849, USA
    Microbiology 154:1616-27. 2008
    ..aeruginosa relies upon to persist within human niches...
  43. pmc Burkholderia cenocepacia requires a periplasmic HtrA protease for growth under thermal and osmotic stress and for survival in vivo
    Ronald S Flannagan
    Department of Microbiology and Immunology, Dental Sciences Building, Rm 3014, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Infect Immun 75:1679-89. 2007
    ..Together, our data demonstrate that HtrA(BCAL2829) is a virulence factor in B. cenocepacia...
  44. pmc Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo
    Tracey A Hunt
    Department of Microbiology and Immunology, Dental Sciences Building, Rm 3014, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Infect Immun 72:4010-22. 2004
    ..This study provides a blueprint to investigate at the molecular level the basis for survival and persistence of B. cenocepacia within the airways...
  45. pmc A simple alfalfa seedling infection model for Pseudomonas aeruginosa strains associated with cystic fibrosis shows AlgT (sigma-22) and RhlR contribute to pathogenesis
    Laura Silo-Suh
    Department of Microbiology and Immunology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond 23298 0678, USA
    Proc Natl Acad Sci U S A 99:15699-704. 2002
    ..These studies also demonstrated that the wounded alfalfa seedling infection model is a useful tool to identify factors contributing to the persistence of P. aeruginosa in CF...