Craig A Simmons

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. pmc Animal models of calcific aortic valve disease
    Krista L Sider
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9
    Int J Inflam 2011:364310. 2011
  2. pmc Spatial heterogeneity of endothelial phenotypes correlates with side-specific vulnerability to calcification in normal porcine aortic valves
    Craig A Simmons
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia PA 19104, USA
    Circ Res 96:792-9. 2005
  3. doi β-catenin mediates mechanically regulated, transforming growth factor-β1-induced myofibroblast differentiation of aortic valve interstitial cells
    Jan Hung Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Arterioscler Thromb Vasc Biol 31:590-7. 2011
  4. doi Hydrogel substrate stiffness and topography interact to induce contact guidance in cardiac fibroblasts
    Shahed Al-Haque
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, M5S 3G9 Toronto, Ontario, Canada
    Macromol Biosci 12:1342-53. 2012
  5. doi The elastic properties of valve interstitial cells undergoing pathological differentiation
    Kristine Wyss
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    J Biomech 45:882-7. 2012
  6. doi A 3D microfluidic platform incorporating methacrylated gelatin hydrogels to study physiological cardiovascular cell-cell interactions
    Michelle B Chen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 13:2591-8. 2013
  7. doi Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformation
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 10:227-34. 2010
  8. doi Methylglyoxal-modified collagen promotes myofibroblast differentiation
    Amy Yuen
    CIHR Group in Matrix Dynamics, University of Toronto, Toronto, ON, Canada
    Matrix Biol 29:537-48. 2010
  9. doi Osteocyte apoptosis is mechanically regulated and induces angiogenesis in vitro
    Wing Yee Cheung
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    J Orthop Res 29:523-30. 2011
  10. doi Integration of statistical modeling and high-content microscopy to systematically investigate cell-substrate interactions
    Wen Li Kelly Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada
    Biomaterials 31:2489-97. 2010

Collaborators

Detail Information

Publications51

  1. pmc Animal models of calcific aortic valve disease
    Krista L Sider
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9
    Int J Inflam 2011:364310. 2011
    ....
  2. pmc Spatial heterogeneity of endothelial phenotypes correlates with side-specific vulnerability to calcification in normal porcine aortic valves
    Craig A Simmons
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia PA 19104, USA
    Circ Res 96:792-9. 2005
    ..These data implicate the endothelium in regulating valvular calcification and suggest that spatial heterogeneity of valvular endothelial phenotypes may contribute to the focal susceptibility for lesion development...
  3. doi β-catenin mediates mechanically regulated, transforming growth factor-β1-induced myofibroblast differentiation of aortic valve interstitial cells
    Jan Hung Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Arterioscler Thromb Vasc Biol 31:590-7. 2011
    ..We elucidated the roles of β-catenin and extracellular matrix stiffness in TGF-β1-induced myofibroblast differentiation of valve interstitial cells (VICs)...
  4. doi Hydrogel substrate stiffness and topography interact to induce contact guidance in cardiac fibroblasts
    Shahed Al-Haque
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, M5S 3G9 Toronto, Ontario, Canada
    Macromol Biosci 12:1342-53. 2012
    ..This report demonstrates a significant interactive effect of substrate stiffness and topography on cardiac fibroblast elongation and orientation using polyacrylamide substrates of different stiffness and topography...
  5. doi The elastic properties of valve interstitial cells undergoing pathological differentiation
    Kristine Wyss
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    J Biomech 45:882-7. 2012
    ..These data demonstrate that VIC phenotypic plasticity and mechanical adaptability are linked and regulated both biomechanically and biochemically, with the potential to influence the progression of CAVD...
  6. doi A 3D microfluidic platform incorporating methacrylated gelatin hydrogels to study physiological cardiovascular cell-cell interactions
    Michelle B Chen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 13:2591-8. 2013
    ..We expect this versatile organ-on-a-chip platform to have broad utility for mechanistic vascular and valvular biology studies and to be useful for drug screening in physiologically-relevant 3D cardiovascular microenvironments...
  7. doi Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformation
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 10:227-34. 2010
    ..More generally, this versatile platform has broad applicability in the fields of mechanobiology, tissue engineering and pathobiology...
  8. doi Methylglyoxal-modified collagen promotes myofibroblast differentiation
    Amy Yuen
    CIHR Group in Matrix Dynamics, University of Toronto, Toronto, ON, Canada
    Matrix Biol 29:537-48. 2010
    ..We conclude that collagen glycation augments the formation and migration of myofibroblasts, critical processes in the development of fibrosis in diabetes...
  9. doi Osteocyte apoptosis is mechanically regulated and induces angiogenesis in vitro
    Wing Yee Cheung
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    J Orthop Res 29:523-30. 2011
    ..Blocking VEGF in apoptotic osteocyte conditioned media abolished tubule formation effects (p < 0.001). Our results suggest that osteocyte apoptosis is flow-regulated and promotes angiogenesis in a VEGF-mediated manner...
  10. doi Integration of statistical modeling and high-content microscopy to systematically investigate cell-substrate interactions
    Wen Li Kelly Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada
    Biomaterials 31:2489-97. 2010
    ....
  11. doi Functional characterization of human coronary artery smooth muscle cells under cyclic mechanical strain in a degradable polyurethane scaffold
    Soroor Sharifpoor
    University of Toronto, Institute of Biomaterials and Biomedical Engineering, Faculty of Dentistry, 124 Edward Street, Toronto, Ontario, Canada M5G1G6
    Biomaterials 32:4816-29. 2011
    ..The study highlights the importance of implementing physiologically-relevant biomechanical stimuli in the development of synthetic elastomeric tissue engineering scaffolds...
  12. doi A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial arrays
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Biomaterials 31:577-84. 2010
    ..This complex mechanical interaction between cells and biomaterials further emphasizes the need for high-throughput approaches to conduct mechanically active experiments in three-dimensional culture...
  13. doi Calcification by valve interstitial cells is regulated by the stiffness of the extracellular matrix
    Cindy Ying Yin Yip
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, ON, Canada
    Arterioscler Thromb Vasc Biol 29:936-42. 2009
    ..In this study, the influence of matrix stiffness in modulating calcification by valve interstitial cells (VICs), and their differentiation to pathological phenotypes was assessed...
  14. doi Osteocyte apoptosis regulates osteoclast precursor adhesion via osteocytic IL-6 secretion and endothelial ICAM-1 expression
    Wing Yee Cheung
    Institute of Biomaterials and Biomedical Engineering, 164 College Street, Room 407, University of Toronto, Toronto, Ontario M5S 3G9
    Bone 50:104-10. 2012
    ....
  15. doi Microdevice array-based identification of distinct mechanobiological response profiles in layer-specific valve interstitial cells
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Integr Biol (Camb) 5:673-80. 2013
    ....
  16. doi Inhibition of pathological differentiation of valvular interstitial cells by C-type natriuretic peptide
    Cindy Y Y Yip
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada
    Arterioscler Thromb Vasc Biol 31:1881-9. 2011
    ..We aimed to determine whether CNP inhibits VIC pathological differentiation as a mechanism to explain its protective effects...
  17. doi Cell-matrix interactions in the pathobiology of calcific aortic valve disease: critical roles for matricellular, matricrine, and matrix mechanics cues
    Jan Hung Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
    Circ Res 108:1510-24. 2011
    ....
  18. doi Lessons from (patho)physiological tissue stiffness and their implications for drug screening, drug delivery and regenerative medicine
    Wen Li Kelly Chen
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada
    Adv Drug Deliv Rev 63:269-76. 2011
    ....
  19. doi A digital microfluidic platform for primary cell culture and analysis
    Suthan Srigunapalan
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 12:369-75. 2012
    ..The ability to manipulate, maintain, and assay primary cells demonstrates a useful application for DMF in studies involving precious samples of cells from small animals or human patients...
  20. doi The aortic valve microenvironment and its role in calcific aortic valve disease
    Cindy Ying Yin Yip
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
    Cardiovasc Pathol 20:177-82. 2011
    ....
  21. doi Comparison of analytical and inverse finite element approaches to estimate cell viscoelastic properties by micropipette aspiration
    Ruogang Zhao
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
    J Biomech 42:2768-73. 2009
    ..These data provide practical guidelines for analysis of MA data that account for the wide range of conditions encountered in typical experiments...
  22. ncbi A simple method to estimate the exponential material parameters of heart valve tissue based on analogy between uniaxial tension and micropipette aspiration
    Ruogang Zhao
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
    Biomech Model Mechanobiol 12:1283-90. 2013
    ..38).Thus, the analytical uniaxial tension model, which avoids solving resource-intensive numerical problems, is as accurate as the FE model in estimating the effective modulus of valve tissue tested by MA...
  23. doi Influence of substrate stiffness on the phenotype of heart cells
    Bashir Bhana
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, 164 College Street, Toronto, Ontario, Canada
    Biotechnol Bioeng 105:1148-60. 2010
    ..3), reasonable ET (ranging from 3.95 +/- 0.8 to 4.4 +/- 0.7 V/cm), high contractile force development (ranging from 0.52 +/- 0.2 to 1.60 +/- 0.6 mN/mm(2)), and well-developed striations, all consistent with a differentiated phenotype...
  24. doi Technique for real-time measurements of endothelial permeability in a microfluidic membrane chip using laser-induced fluorescence detection
    Edmond W K Young
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada, M5S 3G8
    Anal Chem 82:808-16. 2010
    ....
  25. doi Boning up on Wolff's Law: mechanical regulation of the cells that make and maintain bone
    Jan Hung Chen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
    J Biomech 43:108-18. 2010
    ....
  26. doi Determination of local and global elastic moduli of valve interstitial cells cultured on soft substrates
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
    J Biomech 46:1967-71. 2013
    ..They also provide new physiologically-relevant data on VIC mechanical properties and their correlation with substrate stiffness-dependent cytoskeletal changes, with relevance to heart valve mechanobiology and disease. ..
  27. doi An improved texture correlation algorithm to measure substrate-cytoskeletal network strain transfer under large compressive strain
    Ruogang Zhao
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
    J Biomech 45:76-82. 2012
    ..The tools and approaches developed here enable improved characterization of cell-matrix interactions under large deformation and in doing so, may reveal new insights into mechanotransduction mechanisms in such circumstances...
  28. pmc Single cell deposition and patterning with a robotic system
    Zhe Lu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 5:e13542. 2010
    ....
  29. doi Measurement of layer-specific mechanical properties in multilayered biomaterials by micropipette aspiration
    Ruogang Zhao
    Institute of Biomaterials and Biomedical Engineering, Department of Mechanical and Industrial Engineering, Faculty of Dentistry, University of Toronto, 164 College Street, Toronto, Ontario, Canada M5S 3G9
    Acta Biomater 7:1220-7. 2011
    ..01), consistent with data obtained by tensile testing of dissected layers. This study provides practical guidelines for the use of MA to measure the mechanical properties of single layers in intact multilayer biomaterials and tissues...
  30. ncbi Techniques for isolating and purifying porcine aortic valve endothelial cells
    Wing Yee Cheung
    Institute of Biomaterials and Biomedical Engineering, Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
    J Heart Valve Dis 17:674-81. 2008
    ..The study aim was to test various isolation protocols to improve the yield and purity of isolated ECs, and to assess two purification techniques to further deplete contaminating ICs and improve the quality of long-term EC cultures...
  31. ncbi Integrating polyurethane culture substrates into poly(dimethylsiloxane) microdevices
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Biomaterials 30:5241-50. 2009
    ..More generally, this technique can improve the impact and applicability of microdevice-based research by facilitating the use of alternate, relevant biomaterials while maintaining the advantages of using PDMS for microdevice fabrication...
  32. doi A study of vascular smooth muscle cell function under cyclic mechanical loading in a polyurethane scaffold with optimized porosity
    Soroor Sharifpoor
    University of Toronto, Institute of Biomaterials and Biomedical Engineering, Faculty of Dentistry, 124 Edward Street, Room 461, Toronto, Ontario M5G1G6, Canada
    Acta Biomater 6:4218-28. 2010
    ....
  33. doi A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditions
    Lindsey K Fiddes
    Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada
    Biomaterials 31:3459-64. 2010
    ..40-100 mum; (ii) the fabrication of microchannels with constrictions, and (iii) the capability to grow endothelial cells on the inner surface of the microchannels...
  34. pmc Design and formulation of functional pluripotent stem cell-derived cardiac microtissues
    Nimalan Thavandiran
    Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9
    Proc Natl Acad Sci U S A 110:E4698-707. 2013
    ..The design criteria identified with our CMW platform should accelerate the development of predictive in vitro assays of human heart tissue function. ..
  35. ncbi Biaxial mechanical testing of human sclera
    Armin Eilaghi
    Department of Mechanical and Industrial Engineering, University of Toronto, Canada
    J Biomech 43:1696-701. 2010
    ..026 and -0.058 in directions 1 and 2, respectively). Human sclera showed heterogeneous, near-isotropic, nonlinear mechanical properties over the scale of our samples...
  36. ncbi In situ mechanical characterization of the cell nucleus by atomic force microscopy
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada M5S 3G8
    ACS Nano 8:3821-8. 2014
    ..This technique has potential to become a reliable quantitative measurement tool for intracellular mechanics studies. ..
  37. pmc Mesenchymal stem cell mechanobiology and emerging experimental platforms
    Luke Macqueen
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    J R Soc Interface 10:20130179. 2013
    ..Our main goals are to provide engineers and microtechnology developers with an up-to-date description of MSC mechanobiology that is relevant to the design of experimental platforms and to introduce biologists to these emerging platforms...
  38. ncbi Miniaturized platform with on-chip strain sensors for compression testing of arrayed materials
    Luke Macqueen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
    Lab Chip 12:4178-84. 2012
    ..The miniaturized platform can significantly increase mechanical testing efficiency, particularly when testing of iterative sample formulations is required...
  39. doi Biophysical characterization of bladder cancer cells with different metastatic potential
    Haijiao Liu
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, M5S 3G8, Canada
    Cell Biochem Biophys 68:241-6. 2014
    ..Quantitative differences in SMC and Young's modulus values of the high-grade and low-grade UCC cells are, for the first time, reported. ..
  40. ncbi Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels
    Edmond W K Young
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada, M5S 3G8
    Lab Chip 7:1759-66. 2007
    ..Furthermore, they reveal important phenotypic differences between vascular and valvular endothelium, with implications for endothelial mechanobiology and the design of microdevices and engineered tissues...
  41. pmc Identification and characterization of aortic valve mesenchymal progenitor cells with robust osteogenic calcification potential
    Jan Hung Chen
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
    Am J Pathol 174:1109-19. 2009
    ..These findings demonstrate that the aortic valve is rich in a mesenchyma l progenitor cell population that has strong potential to contribute to valve calcification...
  42. ncbi A rapid, reliable method to isolate high quality endothelial RNA from small spatially-defined locations
    Craig A Simmons
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104 6383, USA
    Ann Biomed Eng 32:1453-9. 2004
    ....
  43. doi Molecular markers of early orthodontic tooth movement
    Patricia Joyce Brooks
    Orthodontic Department, Faculty of Dentistry, University of Toronto, Toronto, Canada
    Angle Orthod 79:1108-13. 2009
    ..To understand the molecular basis of early orthodontic tooth movement by looking at the expression of KI-67, runt-related transcription factor 2 (Runx2), and tumor necrosis factor ligand superfamily member 11 (RANKL) proteins...
  44. doi (Micro)managing the mechanical microenvironment
    Christopher Moraes
    Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
    Integr Biol (Camb) 3:959-71. 2011
    ....
  45. doi Effects of scleral stiffness properties on optic nerve head biomechanics
    Armin Eilaghi
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Canada
    Ann Biomed Eng 38:1586-92. 2010
    ..Estimations of the deformation at the optic nerve head region, particularly at elevated IOP, should take into account the nonlinear nature of scleral stiffness...
  46. doi Augmenting microgel flow via receptor-ligand binding in the constrained geometries of microchannels
    Lindsey K Fiddes
    Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario, Canada M5S 3H6
    Lab Chip 9:286-90. 2009
    ....
  47. doi Substrate architecture and fluid-induced shear stress during chondrocyte seeding: role of alpha5beta1 integrin
    Caroline G Spiteri
    CIHR BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada
    Biomaterials 29:2477-89. 2008
    ..Identifying the substrate-induced mechanical and molecular mechanisms that influence chondrocyte behaviour and tissue formation may ultimately lead to the formation of a tissue that more closely resembles natural articular cartilage...
  48. ncbi Aortic valve: turning over a new leaf(let) in endothelial phenotypic heterogeneity
    Peter F Davies
    Arterioscler Thromb Vasc Biol 24:1331-3. 2004
  49. ncbi Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro
    William L Murphy
    Departments of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Biomaterials 26:303-10. 2005
    ..Taken together, these data support the concept that substrate signals significantly influence MSC growth and differentiation, highlighting the importance of carrier material composition in stem cell-based tissue engineering schemes...
  50. ncbi Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells
    Craig A Simmons
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Bone 35:562-9. 2004
    ..These data demonstrate that appropriate combinations of soluble and biomaterial-mediated regulatory signals in cell-based tissue engineering systems can result in both more efficient and more effective tissue regeneration...
  51. ncbi Mechanobiology of the aortic heart valve
    Jonathan T Butcher
    Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA
    J Heart Valve Dis 17:62-73. 2008
    ..Finally, the means by which organ culture systems are being utilized to study heart valve biology, thereby providing a complementary approach to in vivo experimentation, are described...