Research Topics
Genomes and GenesSpecies | Craig A SimmonsSummaryAffiliation: University of Toronto Country: Canada Publications
| Collaborators
|
Detail Information
Publications
Spatial heterogeneity of endothelial phenotypes correlates with side-specific vulnerability to calcification in normal porcine aortic valvesCraig A Simmons
Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia PA 19104, USA
Circ Res 96:792-9. 2005..These data implicate the endothelium in regulating valvular calcification and suggest that spatial heterogeneity of valvular endothelial phenotypes may contribute to the focal susceptibility for lesion development...
β-catenin mediates mechanically regulated, transforming growth factor-β1-induced myofibroblast differentiation of aortic valve interstitial cellsJan Hung Chen
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Arterioscler Thromb Vasc Biol 31:590-7. 2011..We elucidated the roles of β-catenin and extracellular matrix stiffness in TGF-β1-induced myofibroblast differentiation of valve interstitial cells (VICs)...- Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformationChristopher Moraes
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada M5S 3G8
Lab Chip 10:227-34. 2010..More generally, this versatile platform has broad applicability in the fields of mechanobiology, tissue engineering and pathobiology... - Calcification by valve interstitial cells is regulated by the stiffness of the extracellular matrixCindy Ying Yin Yip
Institute of Biomaterials and Biomedical Engineering, University of Toronto, ON, Canada
Arterioscler Thromb Vasc Biol 29:936-42. 2009..In this study, the influence of matrix stiffness in modulating calcification by valve interstitial cells (VICs), and their differentiation to pathological phenotypes was assessed... - Integration of statistical modeling and high-content microscopy to systematically investigate cell-substrate interactionsWen Li Kelly Chen
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada
Biomaterials 31:2489-97. 2010.... - A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial arraysChristopher Moraes
Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
Biomaterials 31:577-84. 2010..This complex mechanical interaction between cells and biomaterials further emphasizes the need for high-throughput approaches to conduct mechanically active experiments in three-dimensional culture... - Influence of substrate stiffness on the phenotype of heart cellsBashir Bhana
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 164 College Street, Toronto, Ontario, Canada
Biotechnol Bioeng 105:1148-60. 2010..3), reasonable ET (ranging from 3.95 +/- 0.8 to 4.4 +/- 0.7 V/cm), high contractile force development (ranging from 0.52 +/- 0.2 to 1.60 +/- 0.6 mN/mm(2)), and well-developed striations, all consistent with a differentiated phenotype... - Comparison of analytical and inverse finite element approaches to estimate cell viscoelastic properties by micropipette aspirationRuogang Zhao
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
J Biomech 42:2768-73. 2009..These data provide practical guidelines for analysis of MA data that account for the wide range of conditions encountered in typical experiments... - Technique for real-time measurements of endothelial permeability in a microfluidic membrane chip using laser-induced fluorescence detectionEdmond W K Young
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada, M5S 3G8
Anal Chem 82:808-16. 2010.... 
Techniques for isolating and purifying porcine aortic valve endothelial cellsWing Yee Cheung
Institute of Biomaterials and Biomedical Engineering, Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada
J Heart Valve Dis 17:674-81. 2008..The study aim was to test various isolation protocols to improve the yield and purity of isolated ECs, and to assess two purification techniques to further deplete contaminating ICs and improve the quality of long-term EC cultures...- Boning up on Wolff's Law: mechanical regulation of the cells that make and maintain boneJan Hung Chen
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
J Biomech 43:108-18. 2010.... - Integrating polyurethane culture substrates into poly(dimethylsiloxane) microdevicesChristopher Moraes
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
Biomaterials 30:5241-50. 2009..More generally, this technique can improve the impact and applicability of microdevice-based research by facilitating the use of alternate, relevant biomaterials while maintaining the advantages of using PDMS for microdevice fabrication... - Inhibition of pathological differentiation of valvular interstitial cells by C-type natriuretic peptideCindy Y Y Yip
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada
Arterioscler Thromb Vasc Biol 31:1881-9. 2011..We aimed to determine whether CNP inhibits VIC pathological differentiation as a mechanism to explain its protective effects... - Biaxial mechanical testing of human scleraArmin Eilaghi
Department of Mechanical and Industrial Engineering, University of Toronto, Canada
J Biomech 43:1696-701. 2010..026 and -0.058 in directions 1 and 2, respectively). Human sclera showed heterogeneous, near-isotropic, nonlinear mechanical properties over the scale of our samples... - A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditionsLindsey K Fiddes
Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada
Biomaterials 31:3459-64. 2010..40-100 mum; (ii) the fabrication of microchannels with constrictions, and (iii) the capability to grow endothelial cells on the inner surface of the microchannels... - Identification and characterization of aortic valve mesenchymal progenitor cells with robust osteogenic calcification potentialJan Hung Chen
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, ON, Canada M5S 3G8
Am J Pathol 174:1109-19. 2009..These findings demonstrate that the aortic valve is rich in a mesenchyma l progenitor cell population that has strong potential to contribute to valve calcification... - Osteocyte apoptosis is mechanically regulated and induces angiogenesis in vitroWing Yee Cheung
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
J Orthop Res 29:523-30. 2011..Blocking VEGF in apoptotic osteocyte conditioned media abolished tubule formation effects (p < 0.001). Our results suggest that osteocyte apoptosis is flow-regulated and promotes angiogenesis in a VEGF-mediated manner... - A rapid, reliable method to isolate high quality endothelial RNA from small spatially-defined locationsCraig A Simmons
Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104 6383, USA
Ann Biomed Eng 32:1453-9. 2004.... - Single cell deposition and patterning with a robotic systemZhe Lu
Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
PLoS ONE 5:e13542. 2010.... - Measurement of layer-specific mechanical properties in multilayered biomaterials by micropipette aspirationRuogang Zhao
Institute of Biomaterials and Biomedical Engineering, Department of Mechanical and Industrial Engineering, Faculty of Dentistry, University of Toronto, 164 College Street, Toronto, Ontario, Canada M5S 3G9
Acta Biomater 7:1220-7. 2011..01), consistent with data obtained by tensile testing of dissected layers. This study provides practical guidelines for the use of MA to measure the mechanical properties of single layers in intact multilayer biomaterials and tissues... - Effects of scleral stiffness properties on optic nerve head biomechanicsArmin Eilaghi
Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Canada
Ann Biomed Eng 38:1586-92. 2010..Estimations of the deformation at the optic nerve head region, particularly at elevated IOP, should take into account the nonlinear nature of scleral stiffness... - Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channelsEdmond W K Young
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario, Canada, M5S 3G8
Lab Chip 7:1759-66. 2007..Furthermore, they reveal important phenotypic differences between vascular and valvular endothelium, with implications for endothelial mechanobiology and the design of microdevices and engineered tissues... - Augmenting microgel flow via receptor-ligand binding in the constrained geometries of microchannelsLindsey K Fiddes
Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario, Canada M5S 3H6
Lab Chip 9:286-90. 2009.... - Molecular markers of early orthodontic tooth movementPatricia Joyce Brooks
Orthodontic Department, Faculty of Dentistry, University of Toronto, Toronto, Canada
Angle Orthod 79:1108-13. 2009..To understand the molecular basis of early orthodontic tooth movement by looking at the expression of KI-67, runt-related transcription factor 2 (Runx2), and tumor necrosis factor ligand superfamily member 11 (RANKL) proteins... - (Micro)managing the mechanical microenvironmentChristopher Moraes
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King s College Road, Toronto, Ontario M5S 3G8, Canada
Integr Biol (Camb) 3:959-71. 2011.... - Animal models of calcific aortic valve diseaseKrista L Sider
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9
Int J Inflam 2011:364310. 2011.... - Cell-matrix interactions in the pathobiology of calcific aortic valve disease: critical roles for matricellular, matricrine, and matrix mechanics cuesJan Hung Chen
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
Circ Res 108:1510-24. 2011.... - The aortic valve microenvironment and its role in calcific aortic valve diseaseCindy Ying Yin Yip
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada
Cardiovasc Pathol 20:177-82. 2011.... - Lessons from (patho)physiological tissue stiffness and their implications for drug screening, drug delivery and regenerative medicineWen Li Kelly Chen
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada
Adv Drug Deliv Rev 63:269-76. 2011.... - Substrate architecture and fluid-induced shear stress during chondrocyte seeding: role of alpha5beta1 integrinCaroline G Spiteri
CIHR BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada
Biomaterials 29:2477-89. 2008..Identifying the substrate-induced mechanical and molecular mechanisms that influence chondrocyte behaviour and tissue formation may ultimately lead to the formation of a tissue that more closely resembles natural articular cartilage... - Methylglyoxal-modified collagen promotes myofibroblast differentiationAmy Yuen
CIHR Group in Matrix Dynamics, University of Toronto, Toronto, ON, Canada
Matrix Biol 29:537-48. 2010..We conclude that collagen glycation augments the formation and migration of myofibroblasts, critical processes in the development of fibrosis in diabetes... - Aortic valve: turning over a new leaf(let) in endothelial phenotypic heterogeneityPeter F Davies
Arterioscler Thromb Vasc Biol 24:1331-3. 2004 - Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cellsCraig A Simmons
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Bone 35:562-9. 2004..These data demonstrate that appropriate combinations of soluble and biomaterial-mediated regulatory signals in cell-based tissue engineering systems can result in both more efficient and more effective tissue regeneration... - Mechanobiology of the aortic heart valveJonathan T Butcher
Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA
J Heart Valve Dis 17:62-73. 2008..Finally, the means by which organ culture systems are being utilized to study heart valve biology, thereby providing a complementary approach to in vivo experimentation, are described... - Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitroWilliam L Murphy
Departments of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Biomaterials 26:303-10. 2005..Taken together, these data support the concept that substrate signals significantly influence MSC growth and differentiation, highlighting the importance of carrier material composition in stem cell-based tissue engineering schemes...