Paul G Scott

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. ncbi request reprint Crystal structure of the biglycan dimer and evidence that dimerization is essential for folding and stability of class I small leucine-rich repeat proteoglycans
    Paul G Scott
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 281:13324-32. 2006
  2. ncbi request reprint Light and X-ray scattering show decorin to be a dimer in solution
    Paul G Scott
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 278:18353-9. 2003
  3. doi request reprint Increased severity of bleomycin-induced skin fibrosis in mice with leukocyte-specific protein 1 deficiency
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    J Invest Dermatol 128:2767-76. 2008
  4. ncbi request reprint Improvement in postburn hypertrophic scar after treatment with IFN-alpha2b is associated with decreased fibrocytes
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Canada
    J Interferon Cytokine Res 27:921-30. 2007
  5. ncbi request reprint Accelerated wound healing in leukocyte-specific, protein 1-deficient mouse is associated with increased infiltration of leukocytes and fibrocytes
    Jianfei Wang
    Wound Healing Research Group Division of Plastic and Reconstructive Surgery and Critical Care, Department of Surgery, University of Alberta, Edmonton, Alberta T6G 2B7, Canada
    J Leukoc Biol 82:1554-63. 2007
  6. doi request reprint Improved scar in postburn patients following interferon-alpha2b treatment is associated with decreased angiogenesis mediated by vascular endothelial cell growth factor
    Jianfei Wang
    Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    J Interferon Cytokine Res 28:423-34. 2008
  7. doi request reprint Deep dermal fibroblasts contribute to hypertrophic scarring
    Jianfei Wang
    1Division of Plastic and Reconstructive Surgery, University of Alberta, Edmonton, Alberta, Canada
    Lab Invest 88:1278-90. 2008
  8. pmc Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan
    Paul G Scott
    Department of Biochemistry and Alberta Synchrotron Institute, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Proc Natl Acad Sci U S A 101:15633-8. 2004
  9. ncbi request reprint Increased TGF-beta-producing CD4+ T lymphocytes in postburn patients and their potential interaction with dermal fibroblasts in hypertrophic scarring
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, AB, Canada
    Wound Repair Regen 15:530-9. 2007
  10. ncbi request reprint Bone marrow-derived stem cells in wound healing: a review
    Yaojiong Wu
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Wound Repair Regen 15:S18-26. 2007

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Crystal structure of the biglycan dimer and evidence that dimerization is essential for folding and stability of class I small leucine-rich repeat proteoglycans
    Paul G Scott
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 281:13324-32. 2006
    ..This implies novel interrelated functions for the parallel beta sheet faces of these leucine-rich repeat proteoglycans, including dimerization and stabilization of protein folding...
  2. ncbi request reprint Light and X-ray scattering show decorin to be a dimer in solution
    Paul G Scott
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 278:18353-9. 2003
    ..6 +/- 0.4 A, a maximum diameter of 98 +/- 5 A, and approximates two intertwined C shapes...
  3. doi request reprint Increased severity of bleomycin-induced skin fibrosis in mice with leukocyte-specific protein 1 deficiency
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    J Invest Dermatol 128:2767-76. 2008
    ..The most likely mechanism for this effect seems to be through an increase in leukocyte infiltration, leading to locally elevated synthesis and the release of chemokines and growth factors...
  4. ncbi request reprint Improvement in postburn hypertrophic scar after treatment with IFN-alpha2b is associated with decreased fibrocytes
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Canada
    J Interferon Cytokine Res 27:921-30. 2007
    ....
  5. ncbi request reprint Accelerated wound healing in leukocyte-specific, protein 1-deficient mouse is associated with increased infiltration of leukocytes and fibrocytes
    Jianfei Wang
    Wound Healing Research Group Division of Plastic and Reconstructive Surgery and Critical Care, Department of Surgery, University of Alberta, Edmonton, Alberta T6G 2B7, Canada
    J Leukoc Biol 82:1554-63. 2007
    ..Further analysis of Lsp1(-/-) mice may suggest ways to improve wound healing and/or treat fibrotic conditions of skin and other tissue...
  6. doi request reprint Improved scar in postburn patients following interferon-alpha2b treatment is associated with decreased angiogenesis mediated by vascular endothelial cell growth factor
    Jianfei Wang
    Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    J Interferon Cytokine Res 28:423-34. 2008
    ..The current in vitro results may provide some insights into the scar improvement that is seen with systemic IFN-alpha2b treatment...
  7. doi request reprint Deep dermal fibroblasts contribute to hypertrophic scarring
    Jianfei Wang
    1Division of Plastic and Reconstructive Surgery, University of Alberta, Edmonton, Alberta, Canada
    Lab Invest 88:1278-90. 2008
    ..Taken together, these data strongly demonstrate that fibroblasts from the deeper layers of the dermis resemble HTS fibroblasts, suggesting that the deeper layer fibroblasts may be critical in the formation of HTS...
  8. pmc Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan
    Paul G Scott
    Department of Biochemistry and Alberta Synchrotron Institute, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Proc Natl Acad Sci U S A 101:15633-8. 2004
    ..This motif seems to be unique to SLRPs and has not been observed in any other LRR protein structure to date. Possible implications of these features for decorin ligand binding and SLRP function are discussed...
  9. ncbi request reprint Increased TGF-beta-producing CD4+ T lymphocytes in postburn patients and their potential interaction with dermal fibroblasts in hypertrophic scarring
    Jianfei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, AB, Canada
    Wound Repair Regen 15:530-9. 2007
    ..Our study suggests that CD4+/TGF-beta-producing T lymphocytes may play an important role in postburn hypertrophic scarring...
  10. ncbi request reprint Bone marrow-derived stem cells in wound healing: a review
    Yaojiong Wu
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Wound Repair Regen 15:S18-26. 2007
    ..Enhanced understanding of these cells may help develop novel therapies for difficult cutaneous conditions such as non-healing chronic wounds and hypertrophic scarring as well as engineering cutaneous substitutes...
  11. doi request reprint Chimeras of bacterial translation factors Tet(O) and EF-G
    Nehal S Thakor
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    FEBS Lett 582:1386-90. 2008
    ..Domains 3-5: (i) are necessary, but not sufficient, for functional specificity; and (ii) modulate GTP hydrolysis by EF-G, while minimally affecting Tet(O), under substrate turnover conditions...
  12. ncbi request reprint An improved procedure for expression and purification of ribosomal protection protein Tet(O) for high-resolution structural studies
    Nehal S Thakor
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alta, Canada T6G 2H7
    Protein Expr Purif 55:388-94. 2007
    ..Here we report molecular cloning, overexpression of His-tagged Tet(O) in E. coli, an improved purification procedure and initial biochemical and biophysical characterization of His-tagged Tet(O)...
  13. ncbi request reprint Cellular and molecular pathology of HTS: basis for treatment
    Alexis Armour
    Department of Surgery, University of Alberta Hospital, Edmonton, Alberta, Canada
    Wound Repair Regen 15:S6-17. 2007
    ..On this background, clinicians are faced with patients who require treatment and the pathophysiologic basis as currently understood is reviewed for a number of emerging modalities...
  14. ncbi request reprint Polarized Th2 cytokine production in patients with hypertrophic scar following thermal injury
    Edward E Tredget
    Wound Healing Research Group, Division of Plastic and Reconstructive Surgery and Critical Care, University of Alberta, Edmonton, Alberta, T6G 2B7, Canada
    J Interferon Cytokine Res 26:179-89. 2006
    ....
  15. ncbi request reprint Skin construct or biological bandage?
    Paul G Scott
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Lancet 366:788-90. 2005
  16. pmc Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM
    Jun Lu
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    EMBO J 25:2930-9. 2006
    ..Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation...
  17. ncbi request reprint Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis
    Yaojiong Wu
    161 HMRC, University of Alberta, 113 Street and 87 Avenue, Edmonton, Alberta T6G 2E1, Canada
    Stem Cells 25:2648-59. 2007
    ..Thus, our data suggest that BM-MSCs promote wound healing through differentiation and release of proangiogenic factors. Disclosure of potential conflicts of interest is found at the end of this article...
  18. ncbi request reprint Identification of fibrocytes in postburn hypertrophic scar
    Liju Yang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Wound Repair Regen 13:398-404. 2005
    ....
  19. ncbi request reprint Development, characterization, and wound healing of the keratin 14 promoted transforming growth factor-beta1 transgenic mouse
    Teddy Chan
    Wound Healing Research Group, University of Alberta, Alberta, Canada
    Wound Repair Regen 10:177-87. 2002
    ....
  20. ncbi request reprint Peripheral blood fibrocytes from burn patients: identification and quantification of fibrocytes in adherent cells cultured from peripheral blood mononuclear cells
    Liju Yang
    Department of Surgery, Wound Healing Research Group, University of Alberta, Edmonton, Alberta, Canada
    Lab Invest 82:1183-92. 2002
    ..Increased TGF-beta in serum stimulates the differentiation of the CD14(+) cell population in PBMC into collagen-producing cells that may be important in wound healing and scarring...
  21. ncbi request reprint Latent and active transforming growth factor beta1 released from genetically modified keratinocytes modulates extracellular matrix expression by dermal fibroblasts in a coculture system
    Barbara S Bauer
    Department of Surgery, Wound Healing Research Group Department of Biochemistry, and Division of Plastic and Reconstructive Surgery, Division of Critical Care, University of Alberta, Edmonton, Canada
    J Invest Dermatol 119:456-63. 2002
    ..005) while also decreasing collagenase mRNA (p < 0.05). These results illustrate the ability of increased levels of transforming growth factor beta1 to override the effects of normal keratinocytes on the behavior of dermal fibroblasts...
  22. ncbi request reprint The JCR:LA-cp rat: a novel model for impaired wound healing
    Barbara S Bauer
    Department of Surgery, Wound Healting Group, University of Alberta, Edmonton, Canada
    Wound Repair Regen 12:86-92. 2004
    ..The JCR:LA-cp rat develops insulin resistance, atherosclerosis and early type 2 diabetes and may be a good model for impairment of wound healing in humans with metabolic syndrome...
  23. ncbi request reprint Degradation of myosin light chain in isolated rat hearts subjected to ischemia-reperfusion injury: a new intracellular target for matrix metalloproteinase-2
    Grzegorz Sawicki
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada
    Circulation 112:544-52. 2005
    ..MMP-2 not only remodels the extracellular matrix but also acts intracellularly in I/R by degrading troponin I. Whether other intracellular targets exist for MMP-2 during I/R is unknown...
  24. ncbi request reprint Fibrocytes from burn patients regulate the activities of fibroblasts
    Jian Fei Wang
    Wound Healing Research Group, Department of Surgery, University of Alberta, Edmonton, AB, Canada
    Wound Repair Regen 15:113-21. 2007
    ..Our results suggest that in healing burn wounds, fibrocytes could regulate the activities of local fibroblasts...
  25. ncbi request reprint Pathophysiology of chronic nonhealing wounds
    Abelardo Medina
    Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, University of Alberta, 8440 112 Street, Edmonton, Alberta, Canada T6G 2B7
    J Burn Care Rehabil 26:306-19. 2005
  26. ncbi request reprint Characterization of opticin and evidence of stable dimerization in solution
    Magali M Le Goff
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences and Research Group in Eye and Vision Science, The Medical School, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:45280-7. 2003
    ..Dimerization could have a number of functional consequences, including divalent ligand interactions...
  27. ncbi request reprint Age-related changes in the proteoglycans of human skin. Specific cleavage of decorin to yield a major catabolic fragment in adult skin
    David A Carrino
    Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:17566-72. 2003
    ..Because of the capacity of decorin to influence collagen fibrillogenesis, catabolism of decorin may have important functional implications with respect to the dermal collagen network...
  28. ncbi request reprint Novel mutations in the small leucine-rich repeat protein/proteoglycan (SLRP) genes in high myopia
    Marja Majava
    Collagen Research Unit, Biocenter and Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Hum Mutat 28:336-44. 2007
    ..This study provides new insight into the genetics of high myopia, suggesting that sequence variations in the SLRP genes expressed in the eye may be among the genetic risk factors underlying the pathogenesis of high myopia...
  29. ncbi request reprint Structural correlations in the family of small leucine-rich repeat proteins and proteoglycans
    Paul A McEwan
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK
    J Struct Biol 155:294-305. 2006
    ..Taken together these results suggest the need for reevaluation of currently accepted models of SLRP interaction with their ligands...
  30. ncbi request reprint Biochemical and immunohistochemical studies of the protein expression and localization of decorin and biglycan in the temporomandibular joint disc of growing rats
    Mikio Kuwabara
    Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Arch Oral Biol 47:473-80. 2002
    ....
  31. ncbi request reprint On the calculation of the binding force between decorin and collagen
    Paul G Scott
    J Biomech 39:1159-60; author reply 1160-2. 2006