John L Rubinstein

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. doi request reprint Angle determination for side views in single particle electron microscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ont, Canada M5G1X8
    J Struct Biol 162:260-70. 2008
  2. pmc Structure of the mitochondrial ATP synthase by electron cryomicroscopy
    John L Rubinstein
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:6182-92. 2003
  3. ncbi request reprint Structural analysis of membrane protein complexes by single particle electron microscopy
    John L Rubinstein
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont, Canada M5G 1X8
    Methods 41:409-16. 2007
  4. doi request reprint Cryo-EM structure of the yeast ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8
    J Mol Biol 382:1256-64. 2008
  5. pmc Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM
    Lindsay A Baker
    Molecular Structure and Function Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 109:11675-80. 2012
  6. doi request reprint Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nature 481:214-8. 2012
  7. doi request reprint Edged watershed segmentation: a semi-interactive algorithm for segmentation of low-resolution maps from electron cryomicroscopy
    Lindsay A Baker
    The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada
    J Struct Biol 176:127-32. 2011
  8. doi request reprint Single particle electron microscopy
    Wilson C Y Lau
    Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada
    Methods Mol Biol 955:401-26. 2013
  9. doi request reprint Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution
    Samir Benlekbir
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Nat Struct Mol Biol 19:1356-62. 2012
  10. pmc Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound V(O) motor
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute and Department of Biochemistry, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Proc Natl Acad Sci U S A 107:1367-72. 2010

Collaborators

Detail Information

Publications17

  1. doi request reprint Angle determination for side views in single particle electron microscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ont, Canada M5G1X8
    J Struct Biol 162:260-70. 2008
    ..We demonstrate how this algorithm works in practice by applying it to a data set of images of affinity-purified bovine mitochondrial ATP synthase...
  2. pmc Structure of the mitochondrial ATP synthase by electron cryomicroscopy
    John L Rubinstein
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:6182-92. 2003
    ....
  3. ncbi request reprint Structural analysis of membrane protein complexes by single particle electron microscopy
    John L Rubinstein
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont, Canada M5G 1X8
    Methods 41:409-16. 2007
    ..This paper seeks to explain how high-quality specimen grids of membrane protein complexes may be prepared to allow for the determination of their structure by EM and image analysis...
  4. doi request reprint Cryo-EM structure of the yeast ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8
    J Mol Biol 382:1256-64. 2008
    ..This new map has allowed construction of a model of subunit arrangement in the F(O) motor of ATP synthase that dictates how dimerization of the complex via subunits e and g might occur...
  5. pmc Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM
    Lindsay A Baker
    Molecular Structure and Function Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 109:11675-80. 2012
    ..The observed bend in the micelle of the detergent-solubilized complex is consistent with previous electron tomography experiments and suggests that monomers of ATP synthase are sufficient to produce curvature in lipid bilayers...
  6. doi request reprint Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nature 481:214-8. 2012
    ..This structure therefore suggests the mechanism by which a transmembrane proton motive force is converted to rotation in rotary ATPases...
  7. doi request reprint Edged watershed segmentation: a semi-interactive algorithm for segmentation of low-resolution maps from electron cryomicroscopy
    Lindsay A Baker
    The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada
    J Struct Biol 176:127-32. 2011
    ..This algorithm has been characterized with experimental and simulated cryo-EM density maps at resolutions between 10 and 25Å...
  8. doi request reprint Single particle electron microscopy
    Wilson C Y Lau
    Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada
    Methods Mol Biol 955:401-26. 2013
    ....
  9. doi request reprint Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution
    Samir Benlekbir
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Nat Struct Mol Biol 19:1356-62. 2012
    ..We provide support for this model by demonstrating that mutation of subunit H to increase the rigidity of the linker between its two domains decreases its ability to inhibit ATPase activity...
  10. pmc Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound V(O) motor
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute and Department of Biochemistry, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Proc Natl Acad Sci U S A 107:1367-72. 2010
    ..The arrangement of structural elements in V(O) gives insight into the mechanism of torque generation from proton translocation...
  11. pmc Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy
    Richard Henderson
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    J Mol Biol 413:1028-46. 2011
    ..A convincing tilt-pair parameter plot, where 60% of the particles show a single cluster around the expected tilt axis and tilt angle, provides confidence in a structure determined using electron cryomicroscopy...
  12. doi request reprint TMaCS: a hybrid template matching and classification system for partially-automated particle selection
    Jianhua Zhao
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    J Struct Biol 181:234-42. 2013
    ..We show that this approach is able to rapidly select particle images from micrographs of a rotary ATPase, a type of membrane protein complex involved in many aspects of biology...
  13. doi request reprint Radiation damage in electron cryomicroscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children, Ontario, Canada
    Methods Enzymol 481:371-88. 2010
    ....
  14. ncbi request reprint ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk region
    John L Rubinstein
    The MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 345:513-20. 2005
    ..Models of the peripheral stalk are proposed that are consistent with this location and with reconstitution experiments conducted with isolated peripheral stalk subunits...
  15. ncbi request reprint Use of phage display and high-density screening for the isolation of an antibody against the 51-kDa subunit of complex I
    John L Rubinstein
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Anal Biochem 314:294-300. 2003
    ..Using this technique, monoclonal scFv and Fab fragments have been produced that bind to the 51-kDa subunit of bovine complex I, a large integral membrane protein complex from mitochondria...
  16. doi request reprint The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architecture
    Andrew J Baldwin
    Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto, ON M5S 1A8, Canada
    Structure 19:1855-63. 2011
    ..The ensuing best-fit structures reveal the polyhedral architecture of αB-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion...
  17. doi request reprint Bacterial polysaccharide co-polymerases share a common framework for control of polymer length
    Ante Tocilj
    Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
    Nat Struct Mol Biol 15:130-8. 2008
    ..Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery...