John L Rubinstein

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. doi request reprint Angle determination for side views in single particle electron microscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ont, Canada M5G1X8
    J Struct Biol 162:260-70. 2008
  2. doi request reprint Alignment of cryo-EM movies of individual particles by optimization of image translations
    John L Rubinstein
    Molecular Structure and Function Program, The Hospital for Sick Children, Canada Department of Medical Biophysics, University of Toronto, Canada Department of Biochemistry, University of Toronto, Canada Electronic address
    J Struct Biol 192:188-95. 2015
  3. pmc Structure of the mitochondrial ATP synthase by electron cryomicroscopy
    John L Rubinstein
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:6182-92. 2003
  4. ncbi request reprint Structural analysis of membrane protein complexes by single particle electron microscopy
    John L Rubinstein
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont, Canada M5G 1X8
    Methods 41:409-16. 2007
  5. doi request reprint Cryo-EM structure of the yeast ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8
    J Mol Biol 382:1256-64. 2008
  6. pmc Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM
    Lindsay A Baker
    Molecular Structure and Function Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 109:11675-80. 2012
  7. doi request reprint Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nature 481:214-8. 2012
  8. pmc Phages have adapted the same protein fold to fulfill multiple functions in virion assembly
    Lia Cardarelli
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada M5S 1A8
    Proc Natl Acad Sci U S A 107:14384-9. 2010
  9. doi request reprint The crystal structure of bacteriophage HK97 gp6: defining a large family of head-tail connector proteins
    Lia Cardarelli
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Mol Biol 395:754-68. 2010
  10. doi request reprint Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution
    Samir Benlekbir
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Nat Struct Mol Biol 19:1356-62. 2012

Collaborators

Detail Information

Publications28

  1. doi request reprint Angle determination for side views in single particle electron microscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ont, Canada M5G1X8
    J Struct Biol 162:260-70. 2008
    ..We demonstrate how this algorithm works in practice by applying it to a data set of images of affinity-purified bovine mitochondrial ATP synthase...
  2. doi request reprint Alignment of cryo-EM movies of individual particles by optimization of image translations
    John L Rubinstein
    Molecular Structure and Function Program, The Hospital for Sick Children, Canada Department of Medical Biophysics, University of Toronto, Canada Department of Biochemistry, University of Toronto, Canada Electronic address
    J Struct Biol 192:188-95. 2015
    ..This individual particle motion correction, in combination with weighting of Fourier components to account for increasing radiation damage in later frames, can be used to improve 3-D maps from single particle cryo-EM...
  3. pmc Structure of the mitochondrial ATP synthase by electron cryomicroscopy
    John L Rubinstein
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 22:6182-92. 2003
    ....
  4. ncbi request reprint Structural analysis of membrane protein complexes by single particle electron microscopy
    John L Rubinstein
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont, Canada M5G 1X8
    Methods 41:409-16. 2007
    ..This paper seeks to explain how high-quality specimen grids of membrane protein complexes may be prepared to allow for the determination of their structure by EM and image analysis...
  5. doi request reprint Cryo-EM structure of the yeast ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8
    J Mol Biol 382:1256-64. 2008
    ..This new map has allowed construction of a model of subunit arrangement in the F(O) motor of ATP synthase that dictates how dimerization of the complex via subunits e and g might occur...
  6. pmc Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM
    Lindsay A Baker
    Molecular Structure and Function Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 109:11675-80. 2012
    ..The observed bend in the micelle of the detergent-solubilized complex is consistent with previous electron tomography experiments and suggests that monomers of ATP synthase are sufficient to produce curvature in lipid bilayers...
  7. doi request reprint Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nature 481:214-8. 2012
    ..This structure therefore suggests the mechanism by which a transmembrane proton motive force is converted to rotation in rotary ATPases...
  8. pmc Phages have adapted the same protein fold to fulfill multiple functions in virion assembly
    Lia Cardarelli
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada M5S 1A8
    Proc Natl Acad Sci U S A 107:14384-9. 2010
    ..Our data also indicate that the addition of a structural element at the N terminus of the lambda head-tail connector protein endows it with a distinctive protein interaction capability compared with many of its putative homologues...
  9. doi request reprint The crystal structure of bacteriophage HK97 gp6: defining a large family of head-tail connector proteins
    Lia Cardarelli
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Mol Biol 395:754-68. 2010
    ..We have also identified gp7 of HK97 as a homologue of gp16 of phage SPP1, which is the second component of the connector of this phage. These proteins are members of another large protein family involved in connector assembly...
  10. doi request reprint Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution
    Samir Benlekbir
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Nat Struct Mol Biol 19:1356-62. 2012
    ..We provide support for this model by demonstrating that mutation of subunit H to increase the rigidity of the linker between its two domains decreases its ability to inhibit ATPase activity...
  11. doi request reprint Edged watershed segmentation: a semi-interactive algorithm for segmentation of low-resolution maps from electron cryomicroscopy
    Lindsay A Baker
    The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada
    J Struct Biol 176:127-32. 2011
    ..This algorithm has been characterized with experimental and simulated cryo-EM density maps at resolutions between 10 and 25Å...
  12. doi request reprint Description and comparison of algorithms for correcting anisotropic magnification in cryo-EM images
    Jianhua Zhao
    Molecular Structure and Function Program, The Hospital for Sick Children, Canada Department of Medical Biophysics, University of Toronto, Canada
    J Struct Biol 192:209-15. 2015
    ..The effect of anisotropic magnification on CTF parameters provides a simple way of detecting magnification anisotropy in cryo-EM datasets. ..
  13. doi request reprint Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase
    Wesley J Errington
    Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario M5S 1A8, Canada
    Structure 20:1141-53. 2012
    ..We propose that adaptor protein self-assembly provides a graded level of regulation of the SPOP/Cul3 E3 ligase toward its multiple protein substrates...
  14. doi request reprint A Bayesian method for 3D macromolecular structure inference using class average images from single particle electron microscopy
    Navdeep Jaitly
    Department of Computer Science, University of Toronto, Toronto, ON, Canada
    Bioinformatics 26:2406-15. 2010
    ..We present a new method for performing ab initio inference of the 3D structures of macromolecules from single particle electron cryo-microscopy experiments using class average images...
  15. doi request reprint TMaCS: a hybrid template matching and classification system for partially-automated particle selection
    Jianhua Zhao
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    J Struct Biol 181:234-42. 2013
    ..We show that this approach is able to rapidly select particle images from micrographs of a rotary ATPase, a type of membrane protein complex involved in many aspects of biology...
  16. doi request reprint Vma9p need not be associated with the yeast V-ATPase for fully-coupled proton pumping activity in vitro
    Stephanie A Bueler
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Biochemistry 54:853-8. 2015
    ..This observation shows that Vma9p is not necessary in vitro for this principal activity of the V-ATPase. ..
  17. doi request reprint Fabrication of carbon films with ∼ 500nm holes for cryo-EM with a direct detector device
    Chelsea R Marr
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Canada
    J Struct Biol 185:42-7. 2014
    ....
  18. doi request reprint Single particle electron microscopy
    Wilson C Y Lau
    Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada
    Methods Mol Biol 955:401-26. 2013
    ....
  19. pmc Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound V(O) motor
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute and Department of Biochemistry, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Proc Natl Acad Sci U S A 107:1367-72. 2010
    ..The arrangement of structural elements in V(O) gives insight into the mechanism of torque generation from proton translocation...
  20. pmc Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy
    Richard Henderson
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    J Mol Biol 413:1028-46. 2011
    ..A convincing tilt-pair parameter plot, where 60% of the particles show a single cluster around the expected tilt axis and tilt angle, provides confidence in a structure determined using electron cryomicroscopy...
  21. doi request reprint Structural Insights into KCTD Protein Assembly and Cullin3 Recognition
    Alan X Ji
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, ON, M5S 1A8, Canada
    J Mol Biol 428:92-107. 2016
    ..Finally, we confirm the 5:5 assembly of KCTD9/Cul3 complexes by cryo-electron microscopy and provide a molecular rationale for BTB-mediated Cul3 binding specificity in the KCTD family. ..
  22. doi request reprint Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase
    Jianhua Zhao
    1 Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, Ontario M5G 0A4, Canada 2 Department of Medical Biophysics, The University of Toronto, Toronto Medical Discovery Tower, MaRS Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada
    Nature 521:241-5. 2015
    ..The structures of these states provide direct evidence that deformation during rotation enables the smooth transmission of power through rotary ATPases. ..
  23. doi request reprint Radiation damage in electron cryomicroscopy
    Lindsay A Baker
    Molecular Structure and Function Program, The Hospital for Sick Children, Ontario, Canada
    Methods Enzymol 481:371-88. 2010
    ....
  24. ncbi request reprint ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk region
    John L Rubinstein
    The MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 345:513-20. 2005
    ..Models of the peripheral stalk are proposed that are consistent with this location and with reconstitution experiments conducted with isolated peripheral stalk subunits...
  25. ncbi request reprint Use of phage display and high-density screening for the isolation of an antibody against the 51-kDa subunit of complex I
    John L Rubinstein
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Anal Biochem 314:294-300. 2003
    ..Using this technique, monoclonal scFv and Fab fragments have been produced that bind to the 51-kDa subunit of bovine complex I, a large integral membrane protein complex from mitochondria...
  26. pmc Phospholipid association is essential for dynamin-related protein Mgm1 to function in mitochondrial membrane fusion
    Jarungjit Rujiviphat
    Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 284:28682-6. 2009
    ..Taken together, our data identify a lipid-binding domain in Mgm1, and the structural analysis suggests a model of how Mgm1 promotes the fusion of opposing mitochondrial inner membranes...
  27. doi request reprint The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architecture
    Andrew J Baldwin
    Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto, ON M5S 1A8, Canada
    Structure 19:1855-63. 2011
    ..The ensuing best-fit structures reveal the polyhedral architecture of αB-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion...
  28. doi request reprint Bacterial polysaccharide co-polymerases share a common framework for control of polymer length
    Ante Tocilj
    Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
    Nat Struct Mol Biol 15:130-8. 2008
    ..Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery...