Genomes and Genes
John L Rubinstein
Affiliation: University of Toronto
- Angle determination for side views in single particle electron microscopyLindsay A Baker
Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ont, Canada M5G1X8
J Struct Biol 162:260-70. 2008..We demonstrate how this algorithm works in practice by applying it to a data set of images of affinity-purified bovine mitochondrial ATP synthase...
- Structure of the mitochondrial ATP synthase by electron cryomicroscopyJohn L Rubinstein
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
EMBO J 22:6182-92. 2003....
- Structural analysis of membrane protein complexes by single particle electron microscopyJohn L Rubinstein
Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont, Canada M5G 1X8
Methods 41:409-16. 2007..This paper seeks to explain how high-quality specimen grids of membrane protein complexes may be prepared to allow for the determination of their structure by EM and image analysis...
- Cryo-EM structure of the yeast ATP synthaseWilson C Y Lau
Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8
J Mol Biol 382:1256-64. 2008..This new map has allowed construction of a model of subunit arrangement in the F(O) motor of ATP synthase that dictates how dimerization of the complex via subunits e and g might occur...
- Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EMLindsay A Baker
Molecular Structure and Function Program, Hospital for Sick Children Research Institute, Toronto, ON, Canada M5G 1X8
Proc Natl Acad Sci U S A 109:11675-80. 2012..The observed bend in the micelle of the detergent-solubilized complex is consistent with previous electron tomography experiments and suggests that monomers of ATP synthase are sufficient to produce curvature in lipid bilayers...
- Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthaseWilson C Y Lau
Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
Nature 481:214-8. 2012..This structure therefore suggests the mechanism by which a transmembrane proton motive force is converted to rotation in rotary ATPases...
- Edged watershed segmentation: a semi-interactive algorithm for segmentation of low-resolution maps from electron cryomicroscopyLindsay A Baker
The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada
J Struct Biol 176:127-32. 2011..This algorithm has been characterized with experimental and simulated cryo-EM density maps at resolutions between 10 and 25Å...
- Single particle electron microscopyWilson C Y Lau
Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada
Methods Mol Biol 955:401-26. 2013....
- Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolutionSamir Benlekbir
Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
Nat Struct Mol Biol 19:1356-62. 2012..We provide support for this model by demonstrating that mutation of subunit H to increase the rigidity of the linker between its two domains decreases its ability to inhibit ATPase activity...
- Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound V(O) motorWilson C Y Lau
Molecular Structure and Function Program, The Hospital for Sick Children Research Institute and Department of Biochemistry, University of Toronto, Toronto, Ontario M5G 1X8, Canada
Proc Natl Acad Sci U S A 107:1367-72. 2010..The arrangement of structural elements in V(O) gives insight into the mechanism of torque generation from proton translocation...
- Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopyRichard Henderson
MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
J Mol Biol 413:1028-46. 2011..A convincing tilt-pair parameter plot, where 60% of the particles show a single cluster around the expected tilt axis and tilt angle, provides confidence in a structure determined using electron cryomicroscopy...
- Radiation damage in electron cryomicroscopyLindsay A Baker
Molecular Structure and Function Program, The Hospital for Sick Children, Ontario, Canada
Methods Enzymol 481:371-88. 2010....
- ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk regionJohn L Rubinstein
The MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
J Mol Biol 345:513-20. 2005..Models of the peripheral stalk are proposed that are consistent with this location and with reconstitution experiments conducted with isolated peripheral stalk subunits...
- Use of phage display and high-density screening for the isolation of an antibody against the 51-kDa subunit of complex IJohn L Rubinstein
MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
Anal Biochem 314:294-300. 2003..Using this technique, monoclonal scFv and Fab fragments have been produced that bind to the 51-kDa subunit of bovine complex I, a large integral membrane protein complex from mitochondria...
- The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architectureAndrew J Baldwin
Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto, ON M5S 1A8, Canada
Structure 19:1855-63. 2011..The ensuing best-fit structures reveal the polyhedral architecture of αB-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion...
- Bacterial polysaccharide co-polymerases share a common framework for control of polymer lengthAnte Tocilj
Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
Nat Struct Mol Biol 15:130-8. 2008..Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery...