Christopher G Proud

Summary

Affiliation: University of British Columbia
Country: Canada

Publications

  1. ncbi Amino acids and mTOR signalling in anabolic function
    C G Proud
    Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Biochem Soc Trans 35:1187-90. 2007
  2. ncbi Regulation of protein synthesis by insulin
    C G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, Canada V6T 1Z3
    Biochem Soc Trans 34:213-6. 2006
  3. ncbi Signalling to translation: how signal transduction pathways control the protein synthetic machinery
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Biochem J 403:217-34. 2007
  4. pmc A sharper instrument for dissecting signalling events: a specific AGC kinase inhibitor
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Biochem J 401:e1-3. 2007
  5. doi The binding of PRAS40 to 14-3-3 proteins is not required for activation of mTORC1 signalling by phorbol esters/ERK
    Bruno D Fonseca
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
    Biochem J 411:141-9. 2008
  6. pmc Re-evaluating the roles of proposed modulators of mammalian target of rapamycin complex 1 (mTORC1) signaling
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver V6T 1Z3, Canada
    J Biol Chem 283:30482-92. 2008
  7. doi Rheb activates protein synthesis and growth in adult rat ventricular cardiomyocytes
    Yanni Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    J Mol Cell Cardiol 45:812-20. 2008
  8. doi Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1
    Vivian H Y Lee
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    FEBS J 275:2185-99. 2008
  9. doi Blocking eukaryotic initiation factor 4F complex formation does not inhibit the mTORC1-dependent activation of protein synthesis in cardiomyocytes
    Brandon P H Huang
    Department of Biochemistry and Molecular Biology, University of British Columbia and Diabetes Research Group, Life Sciences Institute, Vancouver, Canada
    Am J Physiol Heart Circ Physiol 296:H505-14. 2009
  10. ncbi Cell signaling. mTOR, unleashed
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Science 318:926-7. 2007

Collaborators

Detail Information

Publications68

  1. ncbi Amino acids and mTOR signalling in anabolic function
    C G Proud
    Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Biochem Soc Trans 35:1187-90. 2007
    ..This does not require the TSC1/2 (tuberous sclerosis complex 1/2) complex, which is involved in the activation of mTORC1, for example, by insulin. Progress in understanding the mechanisms responsible for this will be reviewed...
  2. ncbi Regulation of protein synthesis by insulin
    C G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, Canada V6T 1Z3
    Biochem Soc Trans 34:213-6. 2006
    ..This is inhibited by rapamycin. Several key questions remain about, for example, the mechanisms by which mTOR controls 4E-BP1 and eEF2 kinase and the control of ribosomal protein translation...
  3. ncbi Signalling to translation: how signal transduction pathways control the protein synthetic machinery
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Biochem J 403:217-34. 2007
    ..However, important questions remain about both the contributions of individual regulatory events to the control of general protein synthesis and the mechanisms by which the translation of specific mRNAs is controlled...
  4. pmc A sharper instrument for dissecting signalling events: a specific AGC kinase inhibitor
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Biochem J 401:e1-3. 2007
    ....
  5. doi The binding of PRAS40 to 14-3-3 proteins is not required for activation of mTORC1 signalling by phorbol esters/ERK
    Bruno D Fonseca
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T1Z3, Canada
    Biochem J 411:141-9. 2008
    ..Indeed, our results suggest that PRAS40 may not actually be involved in controlling mTORC1, but rather be a downstream target of mTORC1 that is regulated in response only to specific stimuli, such as insulin...
  6. pmc Re-evaluating the roles of proposed modulators of mammalian target of rapamycin complex 1 (mTORC1) signaling
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver V6T 1Z3, Canada
    J Biol Chem 283:30482-92. 2008
    ..Our data also indicate that, in the mammalian cell lines tested here, neither TCTP nor FKBP38 regulates mTORC1 signaling...
  7. doi Rheb activates protein synthesis and growth in adult rat ventricular cardiomyocytes
    Yanni Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    J Mol Cell Cardiol 45:812-20. 2008
    ..Our findings reveal that Rheb itself can activate both protein synthesis and cell growth in ARVC and demonstrate the key role played by mTORC1 in the growth of cardiomyocytes...
  8. doi Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1
    Vivian H Y Lee
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    FEBS J 275:2185-99. 2008
    ..These data provide important information on the structural requirements for efficient signalling downstream of mTORC1...
  9. doi Blocking eukaryotic initiation factor 4F complex formation does not inhibit the mTORC1-dependent activation of protein synthesis in cardiomyocytes
    Brandon P H Huang
    Department of Biochemistry and Molecular Biology, University of British Columbia and Diabetes Research Group, Life Sciences Institute, Vancouver, Canada
    Am J Physiol Heart Circ Physiol 296:H505-14. 2009
    ..Therefore, other mTORC1 targets are more important in the inhibition by rapamycin of the rapid activation of protein synthesis and of cell growth...
  10. ncbi Cell signaling. mTOR, unleashed
    Christopher G Proud
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Science 318:926-7. 2007
  11. ncbi Methods for studying signal-dependent regulation of translation factor activity
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada
    Methods Enzymol 431:113-42. 2007
    ..In addition, we provide guidelines on using small molecule inhibitors to assess the involvement of specific signaling pathways in controlling translation factors and protein synthesis...
  12. pmc Screen for chemical modulators of autophagy reveals novel therapeutic inhibitors of mTORC1 signaling
    Aruna D Balgi
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 4:e7124. 2009
    ..Addressing the therapeutic potential of modulating mTORC1 signaling and autophagy in human disease requires active chemicals with pharmacologically desirable properties...
  13. doi The C-terminal domain of Mnk1a plays a dual role in tightly regulating its activity
    Susan Goto
    Department of Biochemistry and Molecular Biology and the Diabetes Research Group, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada
    Biochem J 423:279-90. 2009
    ..These studies help to identify the features that give rise to the diverse properties of human Mnk isoforms...
  14. pmc A novel mechanism for the control of translation initiation by amino acids, mediated by phosphorylation of eukaryotic initiation factor 2B
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
    Mol Cell Biol 28:1429-42. 2008
    ..These findings identify a new way in which amino acids regulate a key step in translation initiation and indicate that this involves a novel amino acid-sensitive signaling mechanism...
  15. pmc Insights into the regulation of eukaryotic elongation factor 2 kinase and the interplay between its domains
    Craig R Pigott
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    Biochem J 442:105-18. 2012
    ..On the basis of these findings, we propose a model for the functional organization and control of eEF2K...
  16. ncbi The mTOR pathway in the control of protein synthesis
    Xuemin Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Physiology (Bethesda) 21:362-9. 2006
    ..mTOR plays key roles in cell physiology. mTOR regulates numerous components involved in protein synthesis, including initiation and elongation factors, and the biogenesis of ribosomes themselves...
  17. doi Severity of vanishing white matter disease does not correlate with deficits in eIF2B activity or the integrity of eIF2B complexes
    Rui Liu
    Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, People s Republic of China
    Hum Mutat 32:1036-45. 2011
    ....
  18. ncbi PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex
    Bruno D Fonseca
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 282:24514-24. 2007
    ..However, this has no effect on the phosphorylation of Akt or TSC2 (an Akt substrate). These data place PRAS40 downstream of mTORC1 but upstream of its effectors, such as S6K1 and 4E-BP1...
  19. doi Oxidized LDL-mediated macrophage survival involves elongation factor-2 kinase
    Johnny H Chen
    Department of Medicine, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada
    Arterioscler Thromb Vasc Biol 29:92-8. 2009
    ..In this report, we investigate the mechanism of oxLDL-mediated macrophage survival...
  20. doi Protein kinase D is a key regulator of cardiomyocyte lipoprotein lipase secretion after diabetes
    Min Suk Kim
    Faculty of Pharmaceutical Sciences, Vancouver, BC, Canada
    Circ Res 103:252-60. 2008
    ..Results from this study could help in restricting cardiac LPL translocation, leading to strategies that overcome contractile dysfunction after diabetes...
  21. ncbi Regulation of mammalian translation factors by nutrients
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, UK
    Eur J Biochem 269:5338-49. 2002
    ..Since control of eIF2B is independent of mTOR, these data indicate the operation of additional, and so far unknown, regulatory mechanisms that control eIF2B activity...
  22. ncbi Translation matters: protein synthesis defects in inherited disease
    Gert C Scheper
    Department of Child Neurology Center for Neurogenomics and Cognitive Research, Vrije Universiteit Medical Center, De Boelelaan 1117, 1081HV Amsterdam, The Netherlands
    Nat Rev Genet 8:711-23. 2007
    ..Given the numerous proteins involved in mRNA translation, it is likely that further inherited diseases will turn out to be caused by mutations in genes that are involved in this complex process...
  23. ncbi The tuberous sclerosis protein TSC2 is not required for the regulation of the mammalian target of rapamycin by amino acids and certain cellular stresses
    Ewan M Smith
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 280:18717-27. 2005
    ..This likely involves the phosphorylation of the elongation factor-2 kinase by the AMP-activated protein kinase...
  24. pmc Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins
    Xuemin Wang
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 25:2558-72. 2005
    ..These data have important implications for understanding signaling downstream of mTOR and the development of new strategies to impair mTOR signaling...
  25. pmc Activation of protein synthesis in cardiomyocytes by the hypertrophic agent phenylephrine requires the activation of ERK and involves phosphorylation of tuberous sclerosis complex 2 (TSC2)
    Mark Rolfe
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, UK
    Biochem J 388:973-84. 2005
    ....
  26. pmc The Drosophila protein kinase LK6 is regulated by ERK and phosphorylates the eukaryotic initiation factor eIF4E in vivo
    Josep L Parra-Palau
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, UK
    Biochem J 385:695-702. 2005
    ..These results show that LK6 binds to ERK and is activated by ERK signalling and it is responsible for phosphorylating eIF4E in Drosophila...
  27. pmc A novel mTOR-regulated phosphorylation site in elongation factor 2 kinase modulates the activity of the kinase and its binding to calmodulin
    Gareth J Browne
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 24:2986-97. 2004
    ..eEF2 kinase is thus a target for mTOR signaling independently of previously known downstream components of the pathway...
  28. pmc Regulation of targets of mTOR (mammalian target of rapamycin) signalling by intracellular amino acid availability
    Anne Beugnet
    School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Scotland, UK
    Biochem J 372:555-66. 2003
    ..These and other data presented in the current study are consistent with the idea that it is intracellular amino acid levels that regulate mTOR signalling...
  29. pmc The C terminus of initiation factor 4E-binding protein 1 contains multiple regulatory features that influence its function and phosphorylation
    Xuemin Wang
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 23:1546-57. 2003
    ..However, in 4E-BP3, replacement of the alanine at the position corresponding to S112 by serine or glutamate did not confer the ability to be released from eIF4E in response to insulin...
  30. ncbi Control of the translational machinery in mammalian cells
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB, CoMPLEX, Dow Street, UK
    Eur J Biochem 269:5337. 2002
  31. ncbi Regulation of the phosphorylation of elongation factor 2 by MEK-dependent signalling in adult rat cardiomyocytes
    Lijun Wang
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee, UK
    FEBS Lett 531:285-9. 2002
    ..Expression of an activated form of MEK leads to dephosphorylation of eEF2, in an mTOR independent manner, indicating that signalling via MEK/Erk suffices to cause dephosphorylation of eEF2...
  32. pmc Evidence that the dephosphorylation of Ser(535) in the epsilon-subunit of eukaryotic initiation factor (eIF) 2B is insufficient for the activation of eIF2B by insulin
    Xuemin Wang
    Division of Molecular Physiology, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 367:475-81. 2002
    ....
  33. ncbi mTOR-mediated regulation of translation factors by amino acids
    Christopher G Proud
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem Biophys Res Commun 313:429-36. 2004
    ..Ser78 is not phosphorylated by known components of the mTOR pathway implying the existence of novel mTOR-regulated kinases that control eEF2 kinase...
  34. ncbi Translational regulation of terminal oligopyrimidine mRNAs induced by serum and amino acids involves distinct signaling events
    Sara Caldarola
    Department of Biology, University Tor Vergata, Rome 00133, Italy
    J Biol Chem 279:13522-31. 2004
    ..Interestingly, rapamycin treatment suggests a novel connection between the mTOR pathway and eukaryotic initiation factor-2alpha phosphorylation in mammalian cells, which may not, however, be involved in TOP mRNA translational regulation...
  35. ncbi Ras/Erk signaling is essential for activation of protein synthesis by Gq protein-coupled receptor agonists in adult cardiomyocytes
    Lijun Wang
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee, UK
    Circ Res 91:821-9. 2002
    ..This study provides new insights into the mechanisms underlying the stimulation of protein synthesis by hypertrophic agents in heart...
  36. pmc Caspase cleavage of initiation factor 4E-binding protein 1 yields a dominant inhibitor of cap-dependent translation and reveals a novel regulatory motif
    Andrew R Tee
    Division of Molecular Physiology, School of Life Sciences, Medical Sciences Institute, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    Mol Cell Biol 22:1674-83. 2002
    ..This suggests that the N-terminal sequence of 4E-BP1 is required for optimal regulation of 4E-BPs by insulin...
  37. pmc Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization
    Taras Valovka
    Ludwig Institute for Cancer Research, London W1W 7BS, United Kingdom
    Mol Cell Biol 23:852-63. 2003
    ..Taken together, this study uncovers a novel mechanism for the regulation of nucleocytoplasmic shuttling of S6KbetaII by PKC-mediated phosphorylation...
  38. ncbi The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha
    Maria Buxade
    Division of Molecular Physiology, College of Life Sciences, University of Dundee, Dundee, UK
    J Biol Chem 283:57-65. 2008
    ..These findings identify a novel Mnk substrate. They also suggest that the Mnk-catalyzed phosphorylation of PSF may regulate the fate of specific mRNAs by modulating their binding to PSF.p54(nrb)...
  39. ncbi Localisation and regulation of the eIF4E-binding protein 4E-BP3
    Miranda Kleijn
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK
    FEBS Lett 532:319-23. 2002
    ..Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus...
  40. ncbi ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling
    Laura E McLeod
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK
    FEBS Lett 531:448-52. 2002
    ..Only at later times is an effect on mTOR signalling observed. These data suggest that energy depletion leads to inhibition of protein synthesis through phosphorylation of eEF2 independently of inhibition of mTOR signalling...
  41. ncbi Mechanisms underlying suppression of protein synthesis induced by transient focal cerebral ischemia in mouse brain
    Thorsten Mengesdorf
    Department of Experimental Neurology, Max Planck Institute for Neurological Research, 50931, Koln, Germany
    Exp Neurol 177:538-46. 2002
    ....
  42. ncbi Regulation of peptide-chain elongation in mammalian cells
    Gareth J Browne
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB complex, Dundee, UK
    Eur J Biochem 269:5360-8. 2002
    ..Conversely, eEF2 is inactivated by phosphorylation in response to stimuli that increase energy demand or reduce its supply. This likely serves to slow down protein synthesis and thus conserve energy under such circumstances...
  43. pmc cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle- and amino acid-dependent manner
    Ewan M Smith
    Division of Molecular Physiology, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 27:1005-16. 2008
    ..These data closely match the control of Ser359 phosphorylation and indicate that cdc2 may be regulated by mTORC1...
  44. ncbi The Mnks: MAP kinase-interacting kinases (MAP kinase signal-integrating kinases)
    Maria Buxade
    Immunopathology Unit, Department of Experimental and Health Sciences, Pompeu Fabra University, Doctor Aiguader 88, 08003 Barcelona, Spain
    Front Biosci 13:5359-73. 2008
    ..Mnks may also control production of inflammatory mediators and signaling from tyrosine kinase receptors, as well as cell proliferation or survival...
  45. ncbi Does phosphorylation of the cap-binding protein eIF4E play a role in translation initiation?
    Gert C Scheper
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, UK
    Eur J Biochem 269:5350-9. 2002
    ..The implications of these studies are discussed in the light of other, in vitro and in vivo, investigations designed to address the role of eIF4E phosphorylation in mRNA translation or its control...
  46. pmc The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes
    Miranda Kleijn
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee, MSI Wellcome Trust Biocentre, DD1 5EH United Kingdom
    BMC Biochem 3:11. 2002
    ..We studied the regulation of protein synthesis after 1 h of activation using alphaCD3 antibody to stimulate the T cell receptor and alphaCD28 antibody to provide the co-stimulus...
  47. ncbi Cellular stresses profoundly inhibit protein synthesis and modulate the states of phosphorylation of multiple translation factors
    Jashmin Patel
    Department of Biosciences, University of Kent at Canterbury, Canterbury, UK
    Eur J Biochem 269:3076-85. 2002
    ..Our data reveal that these stress-inducing agents, which are widely used to study stress-signalling in mammalian cells, exert multiple and complex inhibitory effects on the translational machinery...
  48. ncbi eIF2 and the control of cell physiology
    Christopher G Proud
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dunde DD15EH, United Kingdom
    Semin Cell Dev Biol 16:3-12. 2005
    ....
  49. ncbi Engineering mRNA translation initiation to enhance transient gene expression in chinese hamster ovary cells
    Michele F Underhill
    Research School of Biosciences, University of Kent at Canterbury, UK
    Biotechnol Prog 19:121-9. 2003
    ..This work therefore forms the basis of a rational strategy to generically up-regulate transient expression of recombinant proteins by simultaneous host cell engineering...
  50. ncbi The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2
    Sonia Paytubi
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Biochem J 409:223-31. 2008
    ..Expression of a mutant of ABC50 in which both sites are mutated to alanine markedly decreased the association of eIF2 with 80S ribosomal and polysomal fractions...
  51. ncbi Intracellular sensing of amino acids in Xenopus laevis oocytes stimulates p70 S6 kinase in a target of rapamycin-dependent manner
    Graham R Christie
    Medical Research Council Nutrient Sensing and Signaling Group, Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland
    J Biol Chem 277:9952-7. 2002
    ....
  52. ncbi The multifaceted role of mTOR in cellular stress responses
    Christopher G Proud
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK
    DNA Repair (Amst) 3:927-34. 2004
    ..However, these responses make physiological sense, as impairment of mTOR signalling under these conditions will tend to inhibit anabolic processes and cell growth and division...
  53. ncbi ANG II activates effectors of mTOR via PI3-K signaling in human coronary smooth muscle cells
    Sassan Hafizi
    Department of Cardiothoracic Surgery, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, Heart Science Centre, Harefield Hospital, Middlesex, United Kingdom
    Am J Physiol Heart Circ Physiol 287:H1232-8. 2004
    ..These signaling mechanisms may mediate the growth-promoting effect of ANG II in human cSMCs...
  54. ncbi Translation factors: in sickness and in health
    Catherine M Abbott
    Medical Genetics, University of Edinburgh, Molecular Medicine Centre, Western General Hospital, Edinburgh, EH4 2XU, UK
    Trends Biochem Sci 29:25-31. 2004
    ..It has been discovered recently that mutations in subunits of eukaryotic initiation factor 2B (eIF2B) underlie the neurodegenerative disease termed 'vanishing white matter'...
  55. ncbi Target of rapamycin (TOR)-signaling and RAIP motifs play distinct roles in the mammalian TOR-dependent phosphorylation of initiation factor 4E-binding protein 1
    Anne Beugnet
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 278:40717-22. 2003
    ..The RAIP motif thus promotes the mTOR-dependent phosphorylation of multiple sites in 4E-BP1 independently of the 4E-BP1/raptor interaction...
  56. ncbi Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1
    Andrew R Tee
    University of Dundee, Medical Sciences Institute Wellcome Building Complex, Dow Street, Dundee DD1 5EH, UK
    FEBS Lett 579:4763-8. 2005
    ..Mutation of Rheb and RhebL1 at N41 impaired their interaction with mTOR, which identifies mTOR as a common downstream target of both Rheb and RhebL1...
  57. ncbi The Mnks are novel components in the control of TNF alpha biosynthesis and phosphorylate and regulate hnRNP A1
    Maria Buxade
    Departament de Fisiologia, Universitat de Barcelona, Spain
    Immunity 23:177-89. 2005
    ..Moreover, Mnk-mediated phosphorylation decreases binding of hnRNP A1 to TNFalpha-ARE in vitro or TNFalpha-mRNA in vivo. Therefore, Mnks are novel players in cytokine regulation and potential new targets for anti-inflammatory therapy...
  58. ncbi Features of the catalytic domains and C termini of the MAPK signal-integrating kinases Mnk1 and Mnk2 determine their differing activities and regulatory properties
    Josep Lluis Parra
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, UK
    J Biol Chem 280:37623-33. 2005
    ..These data indicate that multiple features determine the activities of the Mnks and thus impact on their ability to phosphorylate physiological substrates such as eukaryotic initiation factor 4E...
  59. pmc Exercise rapidly increases eukaryotic elongation factor 2 phosphorylation in skeletal muscle of men
    Adam J Rose
    Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, Department of Human Physiology, Copenhagen University, Denmark
    J Physiol 569:223-8. 2005
    ..Given that eEF2 phosphorylation inhibits eEF2 activity and mRNA translation, these findings suggest that the inhibition of protein synthesis in contracting skeletal muscle is due to the Ca(2)(+)-induced stimulation of eEF2 kinase...
  60. pmc The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization
    Gert C Scheper
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 23:5692-705. 2003
    ..Within the nucleus, Mnk2b and certain variants of Mnk2a that are present in the nucleus colocalize with the promyelocytic leukemia protein PML, which also binds to eIF4E...
  61. ncbi Defective translation initiation causes vanishing of cerebral white matter
    Gert C Scheper
    Department of Pediatrics, VU University Medical Center, 1081 HV Amsterdam, The Netherlands
    Trends Mol Med 12:159-66. 2006
    ..Here, we discuss the mechanisms that might be responsible for the selective involvement of the brain white matter in VWM. At present, VWM research is in need of an animal model to study disease mechanisms and therapeutic interventions...
  62. ncbi Quantitative proteomics identifies Gemin5, a scaffolding protein involved in ribonucleoprotein assembly, as a novel partner for eukaryotic initiation factor 4E
    Ivo Fierro-Monti
    Department of Biochemistry and Molecular Biology, University of Southern Denmark
    J Proteome Res 5:1367-78. 2006
    ..Our results demonstrate that our quantitative proteomic strategy can be applied to the identification and quantitation of protein complex components in human cells grown under different conditions...
  63. pmc Muscarinic receptor-mediated activation of p70 S6 kinase 1 (S6K1) in 1321N1 astrocytoma cells: permissive role of phosphoinositide 3-kinase
    Xiuwen Tang
    Division of Cell Signalling, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 374:137-43. 2003
    ..Moreover, 4E-BP1 and hence, presumably, mTOR can be regulated independently of PI3K activation through these mechanisms...
  64. ncbi The rapid activation of protein synthesis by growth hormone requires signaling through mTOR
    Amanda A Hayashi
    Institute of Food Nutrition and Human Health, Massey University, and Metabolism and Microbial Genomics Section, AgResearch Limited, Palmerston North, New Zealand
    Am J Physiol Endocrinol Metab 292:E1647-55. 2007
    ..These results demonstrate for the first time that mTORC1 plays a major role in the rapid activation of protein synthesis by GH...
  65. pmc Ca(2+)-independent protein kinase C activity is required for alpha1-adrenergic-receptor-mediated regulation of ribosomal protein S6 kinases in adult cardiomyocytes
    Lijun Wang
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 373:603-11. 2003
    ..Our data thus suggest that Ca(2+)-independent PKC isoforms play an important role in coupling the alpha(1)-adrenergic receptor to mTOR signalling and protein synthesis in adult cardiomyocytes...
  66. ncbi Shut-down of translation, a global neuronal stress response: mechanisms and pathological relevance
    Wulf Paschen
    Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Pharm Des 13:1887-902. 2007
    ....
  67. pmc Mutations linked to leukoencephalopathy with vanishing white matter impair the function of the eukaryotic initiation factor 2B complex in diverse ways
    Wei Li
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 24:3295-306. 2004
    ..We provide evidence that expression of VWM mutant eIF2B may enhance the translation of specific mRNAs. The variability of the clinical phenotype in VWM may reflect the multiple ways in which VWM mutations affect eIF2B function...
  68. ncbi Ras, PI3-kinase and mTOR signaling in cardiac hypertrophy
    Christopher G Proud
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Cardiovasc Res 63:403-13. 2004
    ....