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Genomes and Genes | Fred PossmayerSummaryAffiliation: University of Western Ontario Country: Canada Publications
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Publications
Surface activity in vitro: role of surfactant proteinsF Possmayer
Department of Ob Gyn, University of Western Ontario, London Health Sciences Centre University Campus, 339 Windermere Road, Ontario, N6A 5A5, London, Canada
Comp Biochem Physiol A Mol Integr Physiol 129:209-20. 2001..SP-A can promote adsorption during film formation, particularly in the presence of SP-B. SP-C appears to have a superior role to SP-B in formation of the surfactant reservoir and in reinsertion of collapse phase lipids...
Recent advances in alveolar biology: some new looks at the alveolar interfaceFred Possmayer
University of Western Ontario, Departments of Obstetrics Gynaecology and Biochemistry, London, Ontario, Canada
Respir Physiol Neurobiol 173:S55-64. 2010..This contribution attempts to emphasize how these new approaches have resulted in a fuller appreciation of events presumably occurring at the alveolar interface...
Atomic force microscopy studies of functional and dysfunctional pulmonary surfactant films, II: albumin-inhibited pulmonary surfactant films and the effect of SP-AYi Y Zuo
Department of Chemistry, University of Western Ontario, London, Ontario, Canada
Biophys J 95:2779-91. 2008..These experimental data indicate a new mechanism of surfactant inhibition by serum proteins, complementing the traditional competitive adsorption mechanism...- Alterations to surfactant precede physiological deterioration during high tidal volume ventilationAdam A Maruscak
Lawson Health Research Institute F4 117, 268 Grosvenor St, London, ON, Canada, N6A 4V2
Am J Physiol Lung Cell Mol Physiol 294:L974-83. 2008..Together, these results suggest that alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time... - A ToF-SIMS study of the lateral organization of lipids and proteins in pulmonary surfactant systemsEleonora Keating
Lawson Health Research Institute, London, Ontario, Canada
Biochim Biophys Acta 1808:614-21. 2011..This may further our understanding of pulmonary surfactant function... - Atomic force microscopy studies of functional and dysfunctional pulmonary surfactant films. I. Micro- and nanostructures of functional pulmonary surfactant films and the effect of SP-AYi Y Zuo
Department of Chemistry, University of Western Ontario, London, Ontario, Canada
Biophys J 94:3549-64. 2008..We conclude that the nanodomains play a predominant role in affecting the biophysical properties of PS monolayers and the monolayer-to-multilayer transition... - P2X7 receptors on osteoblasts couple to production of lysophosphatidic acid: a signaling axis promoting osteogenesisNattapon Panupinthu
Canadian Institutes of Health Research Group in Skeletal Development and Remodeling, London, ON, Canada
J Cell Biol 181:859-71. 2008..We conclude that P2X7 receptors enhance osteoblast function through a cell-autonomous mechanism. Furthermore, a novel signaling axis links P2X7 receptors to production of LPA and COX metabolites, which in turn stimulate osteogenesis... - Reactive oxygen species inactivation of surfactant involves structural and functional alterations to surfactant proteins SP-B and SP-CKarina Rodriguez-Capote
Department of Obstetrics Gynaecology, Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, University of Western Ontario, London, Ontario, Canada
Biophys J 90:2808-21. 2006.... - The effects of hyperoxia exposure on lung function and pulmonary surfactant in a rat model of acute lung injuryPatrick W Pace
Department of Physiology and Pharmacology, Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada
Exp Lung Res 35:380-98. 2009.... - Disparate effects of two phosphatidylcholine binding proteins, C-reactive protein and surfactant protein A, on pulmonary surfactant structure and functionKaushik Nag
Department of Chemistry, Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, University of Western Ontario, London, Ontario, Canada N6A 5A5
Am J Physiol Lung Cell Mol Physiol 287:L1145-53. 2004..These studies indicate that although SP-A and CRP both bind PC, there is a difference in the manner in which they interact with surface films... - Hyperoxia exposure impairs surfactant function and metabolismHiromi Zenri
Department of Medicine, Lawson Health Research Institute, St. Joseph's Health Centre, The University of Western Ontario, London, Ontario, Canada
Crit Care Med 32:1155-60. 2004..CONCLUSIONS: Oxidative stress on the endogenous surfactant system may represent an important mechanism contributing to the surfactant dysfunction and abnormal surfactant metabolism associated with hyperoxia-induced lung injury... - Atomic force microscopy analysis of rat pulmonary surfactant filmsXiujun Jiao
Lawson Health Research Institute, University of Western Ontario, London, Canada
Biophys Chem 158:119-25. 2011..Overall, the data indicate that surfactant films demonstrate phase separation at low surface pressures and multilayer formation at higher pressure, features likely important for normal surfactant function... - Current perspectives in pulmonary surfactant--inhibition, enhancement and evaluationYi Y Zuo
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
Biochim Biophys Acta 1778:1947-77. 2008.... - Effect of cholesterol on the biophysical and physiological properties of a clinical pulmonary surfactantEleonora Keating
Department of Chemistry, Surface Science Western, Western Science Center, Lawson Health Research Institute, University of Western Ontario, London, Canada
Biophys J 93:1391-401. 2007..We used time-of-flight-secondary ion mass spectrometry and principal component analysis to show that cholesterol is concentrated in the Lo phase, where it induces structural changes... - Protein inhibition of surfactant during mechanical ventilation of isolated rat lungsKeith Da Silva
Lawson Health Research Institute and Department of Physiology, The University of Western Ontario, London, Ontario, Canada
Exp Lung Res 31:745-58. 2005..It is concluded that during mechanical ventilation, leakage of proteins results in surfactant inhibition and thereby contribute to decreased lung compliance... - Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometrySuya Liu
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
Rapid Commun Mass Spectrom 22:197-203. 2008..The sequence of SP-C determined here also reveals an L to V substitution at its position 22 compared with the published bovine SP-B sequence... - A modified squeeze-out mechanism for generating high surface pressures with pulmonary surfactantEleonora Keating
Lawson Health Research Institute, The University of Western Ontario, London, Ontario, Canada
Biochim Biophys Acta 1818:1225-34. 2012..This allows the surface film to attain low surface tensions during compression and maintain values near equilibrium during expansion... - Metal nanoparticle pollutants interfere with pulmonary surfactant function in vitroMandeep Singh Bakshi
Department of Obstetrics and Gynaecology, University of Western Ontario, Schulich School of Medicine and Dentistry, London, Ontario, Canada
Biophys J 94:855-68. 2008..It is concluded that Au NP can interact with and sequester pulmonary surfactant phospholipids and, if inhaled from the atmosphere, could impede pulmonary surfactant function in the lung... - Physiological and inflammatory response to instillation of an oxidized surfactant in a rat model of surfactant deficiencyTimothy C Bailey
Department of Physiology and Pharmacology, Lawson Health Research Institute, University of Western Ontario, London, ON, Canada N6A 4V2
J Appl Physiol 96:1674-80. 2004..We conclude that instillation of an in vitro oxidized surfactant causes an inferior physiological response in a surfactant-deficient rat... - The enteric bacterial metabolite propionic acid alters brain and plasma phospholipid molecular species: further development of a rodent model of autism spectrum disordersRaymond H Thomas
The Kilee Patchell Evans Autism Research Group, Department of Psychology, University of Western Ontario, London, ON, N6A 5C2, Canada
J Neuroinflammation 9:153. 2012.... - Mechanical ventilation of isolated rat lungs changes the structure and biophysical properties of surfactantRuud A W Veldhuizen
Lawson Health Research Institute and Department of Physiology, University of Western Ontario, London, Ontario, Canada N6A 4V2
J Appl Physiol 92:1169-75. 2002..We conclude that the injurious mechanical ventilation impairs LA function and that this impairment is associated with significant morphological alterations... - Segregation of saturated chain lipids in pulmonary surfactant films and bilayersKaushik Nag
Department of Obstetrics and Gynecology, University of Western Ontario, London, Ontario N6A 5A5, Canada
Biophys J 82:2041-51. 2002.... - Phospholipid metabolism in lung surfactantRuud Veldhuizen
Departments of Medicine and Physiology and Pharmacology, Lawson Health Research Institute, London, ON, N6A 4V2, Canada
Subcell Biochem 37:359-88. 2004..Third, the surface film is converted to a small vesicular form. Finally, these small vesicles are taken-up by the type II cells for recycling and degradation and by alveolar macrophages for degradation... - Retinoic acid is a key regulatory switch determining the difference between lung and thyroid fates in Xenopus laevisJean H Wang
Children s Health Research Institute, London, Ontario, Canada
BMC Dev Biol 11:75. 2011..The lung and thyroid are derived from the anterior endoderm. Retinoic acid and Fgf signalling are known to be essential for development of the lung in mouse but little is known on how the lung and thyroid are specified in Xenopus... - Altered brain phospholipid and acylcarnitine profiles in propionic acid infused rodents: further development of a potential model of autism spectrum disordersRaymond H Thomas
The Kilee Patchell Evans Autism Research Group, Department of Psychology and Biology, University of Western Ontario, London, Ontario, Canada
J Neurochem 113:515-29. 2010..These results provide evidence of a relationship between changes in brain lipid profiles and the occurrence of ASD-like behaviors using the autism rodent model. We propose that altered brain fatty acid metabolism may contribute to ASD... - Lamellar phase supported synthesis of colloidal gold nanoparticles, nanoclusters, and nanowiresMandeep Singh Bakshi
Department of Ob Gyn and Biochemistry, University of Western Ontario, London, ON, Canada
J Nanosci Nanotechnol 7:916-24. 2007..The presence of CYC induces a tendency to form nanowire and it is more prominent in the case of surfactants with longer spacer group... - Acute lung injury and lung transplantation influence in vitro subtype conversion of pulmonary surfactantGayatri Maitra
Medical Research Council Group in Fetal and Neonatal Health and Development, Department of Biochemistry and Obstetrics and Gynaecology, University of Western Ontario, London N6A 5A5, Ontario, Canada N6A 4V2
Am J Physiol Lung Cell Mol Physiol 282:L67-74. 2002..LA from all experimental groups had similar protein levels. These studies show acute lung injury, transplant, ischemia-reperfusion, and surfactant treatment have major effects on surfactant subtype integrity... - The effect of repeated umbilical cord occlusions on pulmonary surfactant protein mRNA levels in the ovine fetusLaura Nardo
Department of Obstetrics and Gynaecology, The Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada
J Soc Gynecol Investig 12:510-7. 2005..CONCLUSION: The surprising decrease in SP-A and SP-B mRNA levels, which contrasts with other studies, suggests intermittent asphyxial episodes impact differently on surfactant apoprotein mRNA expression than does prolonged hypoxia... - Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolaseMeera Nanjundan
Department of Obstetrics and Gynaecology, Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, The University of Western Ontario, 339 Windermere Road, London, Ontario, Canada N6A 5A5
Am J Physiol Lung Cell Mol Physiol 284:L1-23. 2003..LPP activity could also influence transdifferentiation of alveolar type II to type I cells. It is considered likely that these lipid phosphohydrolases have critical roles in lung morphogenesis and in acute lung injury and repair... - Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRKAdel I Aziziyeh
Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada
Cell Signal 21:1207-17. 2009..Taken together, these results establish RalA and GRK2 as key regulators of LPA receptor signalling and demonstrate for the first time that LPA(1) activity facilitates the formation of a novel protein complex between these two proteins... - P2X7 nucleotide receptors mediate blebbing in osteoblasts through a pathway involving lysophosphatidic acidNattapon Panupinthu
Canadian Institutes of Health Research Group in Skeletal Development and Remodeling, Department of Physiology and Pharmacology, The University of Western Ontario, London, Canada
J Biol Chem 282:3403-12. 2007..This pathway may contribute to P2X7-stimulated osteogenesis during skeletal development and mechanotransduction... - Pulmonary surfactant protein-A (SP-A) restores the surface properties of surfactant after oxidation by a mechanism that requires the Cys6 interchain disulfide bond and the phospholipid binding domainKarina Rodriguez Capote
Departments of Obstetrics Gynecology and Biochemistry, Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, University of Western Ontario, London N6A 5A5, Canada
J Biol Chem 278:20461-74. 2003....