Johann Dd Pitout

Summary

Affiliation: University of Calgary
Country: Canada

Publications

  1. ncbi request reprint Enterobacteriaceae that produce extended-spectrum β-lactamases and AmpC β-lactamases in the community: the tip of the iceberg?
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Curr Pharm Des 19:257-63. 2013
  2. doi request reprint Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Expert Rev Anti Infect Ther 10:1165-76. 2012
  3. pmc Population-based laboratory surveillance of Hafnia alvei isolates in a large Canadian health region
    Kevin B Laupland
    Department of Medicine, University of Calgary and Calgary Health Region, Calgary Alberta, Canada
    Ann Clin Microbiol Antimicrob 5:12. 2006
  4. pmc Population-based laboratory surveillance for AmpC beta-lactamase-producing Escherichia coli, Calgary
    Johann D D Pitout
    University of Calgary, Calgary, Alberta, Canada
    Emerg Infect Dis 13:443-8. 2007
  5. doi request reprint Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Lancet Infect Dis 8:159-66. 2008
  6. doi request reprint Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
    J Antimicrob Chemother 61:999-1002. 2008
  7. doi request reprint Antimicrobial susceptibility of well-characterised multiresistant CTX-M-producing Escherichia coli: failure of automated systems to detect resistance to piperacillin/tazobactam
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Int J Antimicrob Agents 32:333-8. 2008
  8. doi request reprint Metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya
    J D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Department of Pathology and Laboratory Medicine, and Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Clin Microbiol Infect 14:755-9. 2008
  9. doi request reprint Multiresistant Enterobacteriaceae: new threat of an old problem
    Johann D D Pitout
    Department of Pathology and Laboratory Medicine, Microbiology and Infectious Diseases, University of Calgary, Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Expert Rev Anti Infect Ther 6:657-69. 2008
  10. pmc Using a commercial DiversiLab semiautomated repetitive sequence-based PCR typing technique for identification of Escherichia coli clone ST131 producing CTX-M-15
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    J Clin Microbiol 47:1212-5. 2009

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Enterobacteriaceae that produce extended-spectrum β-lactamases and AmpC β-lactamases in the community: the tip of the iceberg?
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Curr Pharm Des 19:257-63. 2013
    ....
  2. doi request reprint Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Expert Rev Anti Infect Ther 10:1165-76. 2012
    ..This review will highlight recent aspects on antimicrobial resistance in ExPEC, including the laboratory detection of these isolates, and describe some treatment options for infections due to antimicrobial-resistant isolates...
  3. pmc Population-based laboratory surveillance of Hafnia alvei isolates in a large Canadian health region
    Kevin B Laupland
    Department of Medicine, University of Calgary and Calgary Health Region, Calgary Alberta, Canada
    Ann Clin Microbiol Antimicrob 5:12. 2006
    ..Hospital-based series have characterized Hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness...
  4. pmc Population-based laboratory surveillance for AmpC beta-lactamase-producing Escherichia coli, Calgary
    Johann D D Pitout
    University of Calgary, Calgary, Alberta, Canada
    Emerg Infect Dis 13:443-8. 2007
    ..In this large Canadian region, AmpC beta-lactamase-producing E. coli is an emerging community pathogen that commonly causes urinary tract infections in older women...
  5. doi request reprint Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Lancet Infect Dis 8:159-66. 2008
    ..Thus, more rapid diagnostic testing of ESBL-producing bacteria and the possible modification of guidelines for community-onset bacteraemia associated with UTIs are required...
  6. doi request reprint Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
    J Antimicrob Chemother 61:999-1002. 2008
    ..A study was designed to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants among clinical isolates of Enterobacteriaceae from the Calgary Health Region (CHR)...
  7. doi request reprint Antimicrobial susceptibility of well-characterised multiresistant CTX-M-producing Escherichia coli: failure of automated systems to detect resistance to piperacillin/tazobactam
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Int J Antimicrob Agents 32:333-8. 2008
    ..coli. We recommend that laboratories using Vitek should employ alternative susceptibility testing methods for TZP before reporting the activity of this agent against ESBL-producing E. coli...
  8. doi request reprint Metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya
    J D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Department of Pathology and Laboratory Medicine, and Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Clin Microbiol Infect 14:755-9. 2008
    ..This study is the first report of VIM-2-producing P. aeruginosa from the African continent...
  9. doi request reprint Multiresistant Enterobacteriaceae: new threat of an old problem
    Johann D D Pitout
    Department of Pathology and Laboratory Medicine, Microbiology and Infectious Diseases, University of Calgary, Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Expert Rev Anti Infect Ther 6:657-69. 2008
    ..Recent developments in the epidemiology, clinical relevance and laboratory detection of infections caused by multiresistant Enterobacteriaceae with these new types of resistance mechanisms will be addressed in this review...
  10. pmc Using a commercial DiversiLab semiautomated repetitive sequence-based PCR typing technique for identification of Escherichia coli clone ST131 producing CTX-M-15
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    J Clin Microbiol 47:1212-5. 2009
    ..The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone...
  11. pmc Molecular characteristics of travel-related extended-spectrum-beta-lactamase-producing Escherichia coli isolates from the Calgary Health Region
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    Antimicrob Agents Chemother 53:2539-43. 2009
    ..Our study suggested that clone ST131 coproducing CTX-M-15, OXA-1, TEM-1, and AAC(6')-Ib-cr and clonally unrelated CTX-M-14 producers have emerged as important causes of community-acquired, travel-related infections...
  12. pmc Molecular characteristics of extended-spectrum-beta-lactamase-producing Escherichia coli isolates causing bacteremia in the Calgary Health Region from 2000 to 2007: emergence of clone ST131 as a cause of community-acquired infections
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, 9, 3535 Research Road NW, Calgary, Alberta, Canada
    Antimicrob Agents Chemother 53:2846-51. 2009
    ..coli isolates; and this is the first study to identify CTX-M-14 in E. coli clone ST131...
  13. doi request reprint Detection of AmpC beta-lactamases in Escherichia coli, Klebsiella spp., Salmonella spp. and Proteus mirabilis in a regional clinical microbiology laboratory
    J D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, AB, Canada
    Clin Microbiol Infect 16:165-70. 2010
    ..and P. mirabilis. This approach is convenient, practical and easy to incorporate into the workflow of a clinical laboratory. False-positive AmpC detection may occur with KPC-producing bacteria when inhibitor-based methods are used...
  14. ncbi request reprint IPSAT P1A, a class A beta-lactamase therapy for the prevention of penicillin-induced disruption to the intestinal microflora
    Johann D D Pitout
    University of Calgary, Calgary Laboratory Services and Department of Pathology and Laboratory Medicine, 3535 Research Road NW, Calgary, Alberta, Canada
    Curr Opin Investig Drugs 10:838-44. 2009
    ....
  15. doi request reprint Infections with extended-spectrum beta-lactamase-producing enterobacteriaceae: changing epidemiology and drug treatment choices
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Drugs 70:313-33. 2010
    ....
  16. pmc Molecular epidemiology over an 11-year period (2000 to 2010) of extended-spectrum β-lactamase-producing Escherichia coli causing bacteremia in a centralized Canadian region
    Gisele Peirano
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    J Clin Microbiol 50:294-9. 2012
    ..coli ST131. This will provide insight into the emergence and spread of this multiresistant sequence type...
  17. ncbi request reprint Development and clinical validation of a molecular diagnostic assay to detect CTX-M-type beta-lactamases in Enterobacteriaceae
    J D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
    Clin Microbiol Infect 13:291-7. 2007
    ..The CTX-M-specific PCR assay was reproducible and easy to use. It can be introduced in a clinical or reference laboratory to track and monitor the spread of organisms producing CTX-M enzymes in the community and hospital settings...
  18. pmc Molecular epidemiology of CTX-M-producing Escherichia coli in the Calgary Health Region: emergence of CTX-M-15-producing isolates
    Johann D D Pitout
    Calgary Laboratory Services, Department of Pathology and Laboratory Medicine, University of Calgary, 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
    Antimicrob Agents Chemother 51:1281-6. 2007
    ..This study illustrates the importance of molecular surveillance in tracking CTX-M-producing E. coli strains in the community and investigating their influx into hospitals...
  19. pmc Molecular epidemiology of metallo-beta-lactamase-producing Pseudomonas aeruginosa in the Calgary Health Region: emergence of VIM-2-producing isolates
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
    J Clin Microbiol 45:294-8. 2007
    ..Our findings suggest that CR and dissemination of MBL clusters among P. aeruginosa populations in large geographic healthcare regions are dynamic processes that require continuous molecular surveillance...
  20. pmc Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, 9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
    J Clin Microbiol 43:3129-35. 2005
    ..We recommend that all IPM-nonsusceptible P. aeruginosa isolates be routinely screened for MBL production using the EDTA disk screen test and that PCR confirmation be performed at a regional laboratory...
  21. pmc Modification of the double-disk test for detection of enterobacteriaceae producing extended-spectrum and AmpC beta-lactamases
    J D D Pitout
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary T3C 0J5, Canada
    J Clin Microbiol 41:3933-5. 2003
    ..MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in enterobacteriaceae...
  22. pmc Association between handling of pet treats and infection with Salmonella enterica serotype newport expressing the AmpC beta-lactamase, CMY-2
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services and Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada
    J Clin Microbiol 41:4578-82. 2003
    ..enterica serotype Newport strains in Canada, this study illustrates the necessity of rapid and accurate laboratory-based surveillance in the identification of novel types of antimicrobial resistance...
  23. ncbi request reprint Emerging gram-negative enteric infections
    Johann D D Pitout
    Department of Pathology and Laboratory Medicine, University of Calgary, 11th Floor, Foothills Medical Centre, 1403 29 Street NW, Calgary, Alberta T2N 2T9, Canada
    Clin Lab Med 24:605-26, vi. 2004
    ..It concludes with suggestions for the implementation of molecular techniques to ensure rapid surveillance and enhanced patient care...
  24. ncbi request reprint Emergence of Enterobacteriaceae producing extended-spectrum beta-lactamases (ESBLs) in the community
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Alberta, Canada
    J Antimicrob Chemother 56:52-9. 2005
    ....
  25. pmc Community-wide outbreaks of clonally related CTX-M-14 beta-lactamase-producing Escherichia coli strains in the Calgary health region
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, 9 3535 Research Rd NW, Calgary, Alberta T2L 2K8, Canada
    J Clin Microbiol 43:2844-9. 2005
    ..Further studies are warranted to investigate whether community-onset diseases caused by ESBL-producing E. coli are related to a point source or transmission within the community...
  26. pmc Virulence factors of Escherichia coli isolates that produce CTX-M-type extended-spectrum beta-lactamases
    Johann D D Pitout
    Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada
    Antimicrob Agents Chemother 49:4667-70. 2005
    ..Our results indicate that CTX-M type predicts phylogenetic background, and the virulence potential of ESBL-producing E. coli isolates is a complex issue, requiring further study and ongoing surveillance...
  27. doi request reprint Molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli from the Chicago area: high prevalence of ST131 producing CTX-M-15 in community hospitals
    Gisele Peirano
    Division of Microbiology, Calgary Laboratory Services, Calgary, AB, Canada
    Int J Antimicrob Agents 36:19-23. 2010
    ..coli clonal complex ST131 producing CTX-M-15, CTX-M-14, OXA-1, TEM-1 and aac(6')-Ib-cr has emerged as an important cause of community-onset urinary tract infections caused by ESBL-producing E. coli isolates in the Chicago area...
  28. doi request reprint Molecular epidemiology of Escherichia coli producing CTX-M beta-lactamases: the worldwide emergence of clone ST131 O25:H4
    Gisele Peirano
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    Int J Antimicrob Agents 35:316-21. 2010
    ....
  29. pmc High prevalence of ST131 isolates producing CTX-M-15 and CTX-M-14 among extended-spectrum-beta-lactamase-producing Escherichia coli isolates from Canada
    Gisele Peirano
    Calgary Laboratory Services, Calgary, Alberta, Canada
    Antimicrob Agents Chemother 54:1327-30. 2010
    ..ST131 is an important cause of community onset urinary tract infections due to ESBL-producing E. coli across Canada...
  30. ncbi request reprint Population-based laboratory surveillance for Escherichia coli-producing extended-spectrum beta-lactamases: importance of community isolates with blaCTX-M genes
    Johann D D Pitout
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary Laboratory Services, and the Centre for Antimicrobial Resistance, Calgary, Alberta, Canada
    Clin Infect Dis 38:1736-41. 2004
    ..2; 95% confidence interval, 3.69-54.84). Strains of E. coli with blaCTX-M genes commonly cause community-onset infections, and women and older patients are at highest risk...
  31. ncbi request reprint Investigation of sources of potential bias in laboratory surveillance for anti-microbial resistance
    Kevin B Laupland
    Department of Medicine, University of Calgary and Calgary Health Region, Calgary, Alberta, Canada
    Clin Invest Med 30:E159-66. 2007
    ..Our objective was to evaluate the potential magnitude of bias in reporting of etiologic agents and their resistance rates associated with inclusion of multiple patient samples and non-random timing and location of sampling...
  32. pmc Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp
    Johann D D Pitout
    Division of Microbiology, Calgary Laboratory Services, Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada
    J Clin Microbiol 42:5715-21. 2004
    ..Following the NCCLS recommendations for ESBL testing, all of the control and clinical strains were detected when screened with cefpodoxime and when both cefotaxime and ceftazidime with clavulanate were used as confirmation tests...
  33. ncbi request reprint Population-based epidemiological study of infections caused by carbapenem-resistant Pseudomonas aeruginosa in the Calgary Health Region: importance of metallo-beta-lactamase (MBL)-producing strains
    Kevin B Laupland
    Department of Medicine, University of Calgary, Centre for Anti microbial Resistance, and Calgary Health Region, Calgary, Alberta, Canada
    J Infect Dis 192:1606-12. 2005
    ....
  34. doi request reprint Community-onset extended-spectrum beta-lactamase (ESBL) producing Escherichia coli: importance of international travel
    Kevin B Laupland
    Department of Medicine, University of Calgary and Calgary Health Region, Calgary, Alberta, Canada
    J Infect 57:441-8. 2008
    ..Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli have emerged as significant causes of community-onset disease. We sought to identify risk factors for acquiring community-onset ESBL-producing E. coli...
  35. ncbi request reprint Development and validation of a molecular beacon probe-based real-time polymerase chain reaction assay for rapid detection of methicillin resistance in Staphylococcus aureus
    Sameer Elsayed
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada
    Arch Pathol Lab Med 127:845-9. 2003
    ..A rapid, real-time, duplex, fluorescent molecular beacon probe-based polymerase chain reaction (PCR) assay was recently developed for the detection of methicillin-resistant Staphylococcus aureus...
  36. pmc Clinical outcome of empiric antimicrobial therapy of bacteremia due to extended-spectrum beta-lactamase producing Escherichia coli and Klebsiella pneumoniae
    Vikas P Chaubey
    Department of Medicine, University of Calgary, Calgary, Canada
    BMC Res Notes 3:116. 2010
    ....
  37. doi request reprint Incidence, risk factors, and outcomes of Klebsiella pneumoniae bacteremia
    Bonnie L Meatherall
    Department of Medicine, University of Calgary and Calgary Laboratory Services, Calgary, Alberta, Canada
    Am J Med 122:866-73. 2009
    ..We sought to describe the incidence of, risk factors for, and outcomes associated with K. pneumoniae bacteremia...
  38. pmc Characteristics of NDM-1-producing Escherichia coli isolates that belong to the successful and virulent clone ST131
    Gisele Peirano
    Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada
    Antimicrob Agents Chemother 55:2986-8. 2011
    ..coli ST101, previously isolated from a patient in Canada. The presence of NDM β-lactamases in a very successful and virulent E. coli sequence type is of concern...
  39. pmc Clinical and molecular characteristics of extended-spectrum-beta-lactamase-producing Escherichia coli causing bacteremia in the Rotterdam Area, Netherlands
    Akke K van der Bij
    1Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, Netherlands
    Antimicrob Agents Chemother 55:3576-8. 2011
    ..The majority of E. coli isolates produced CTX-M-15, and 4 sequence types (ST38, ST131, ST405, and ST648) predominated. There were significant differences in clinical and molecular characteristics between the 2 community hospitals...
  40. pmc Use of beta-lactamase inhibitors in disk tests to detect plasmid-mediated AmpC beta-lactamases
    Jennifer A Black
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA
    J Clin Microbiol 42:2203-6. 2004
    ....
  41. pmc Community-onset disease caused by Citrobacter freundii producing a novel CTX-M beta-lactamase, CTX-M-30, in Canada
    Baha Abdalhamid
    Center for Research in Antiinfectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA
    Antimicrob Agents Chemother 48:4435-7. 2004
    ..Sequencing of PCR products by use of CTX-M-specific primers revealed a new combination of four amino acid substitutions. This new gene was designated bla(CTX-M-30) and was encoded on a 3-kb plasmid. The pI of CTX-M-30 was 8.0...
  42. pmc New and sensitive assay for determining Pseudomonas aeruginosa metallo-beta-lactamase resistance to imipenem
    Jingyuan Tan
    Department of Cell and Systems Biology, University of Toronto, Toronto, Canada
    J Clin Microbiol 46:1870-2. 2008
    ..It is appropriate for both high-accuracy screens and laboratories in developing countries with limited resources...
  43. pmc Evaluation of beta-lactamase inhibitors in disk tests for detection of plasmid-mediated AmpC beta-lactamases in well-characterized clinical strains of Klebsiella spp
    Jennifer A Black
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA
    J Clin Microbiol 43:4168-71. 2005
    ..The combination of Syn 2190 and CTT had a sensitivity of 91%, a specificity of 100%, and a reproducibility of 100% and showed the best potential of using an inhibitor for detection of Klebsiella spp. producing pAmpCs...
  44. pmc In vivo selection of fluoroquinolone-resistant Escherichia coli isolates expressing plasmid-mediated quinolone resistance and expanded-spectrum beta-lactamase
    Laurent Poirel
    Service de Bacteriologie Virologie, Hopital de Bicetre, Faculte de Medecine Paris Sud, Universite Paris XI, 94275 Le Kremlin Bicetre cedex, France
    Antimicrob Agents Chemother 50:1525-7. 2006
    ..Both isolates harbored a plasmid-encoded sul1-type integron with qnrA1 and blaVEB-1 genes. Isolate 1B had amino acid substitutions in gyrase and topoisomerase...