J I Nagy

Summary

Affiliation: University of Manitoba
Country: Canada

Publications

  1. pmc Evidence for connexin36 localization at hippocampal mossy fiber terminals suggesting mixed chemical/electrical transmission by granule cells
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 745 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0J9
    Brain Res 1487:107-22. 2012
  2. pmc Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Glia 44:205-18. 2003
  3. ncbi request reprint Connexin26 in adult rodent central nervous system: demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3J7
    J Comp Neurol 441:302-23. 2001
  4. pmc Astrocyte and oligodendrocyte connexins of the glial syncytium in relation to astrocyte anatomical domains and spatial buffering
    James I Nagy
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Cell Commun Adhes 10:401-6. 2003
  5. ncbi request reprint Update on connexins and gap junctions in neurons and glia in the mammalian nervous system
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue Winnipeg, Manitoba, Canada R3E 3J7
    Brain Res Brain Res Rev 47:191-215. 2004
  6. ncbi request reprint A brain slice model for in vitro analyses of astrocytic gap junction and connexin43 regulation: actions of ischemia, glutamate and elevated potassium
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Eur J Neurosci 12:4567-72. 2000
  7. ncbi request reprint Connexins and gap junctions of astrocytes and oligodendrocytes in the CNS
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Brain Res Brain Res Rev 32:29-44. 2000
  8. pmc Connexin26 expression in brain parenchymal cells demonstrated by targeted connexin ablation in transgenic mice
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 745 Bannatyne Ave, Winnipeg, MB, Canada R3E 0J9
    Eur J Neurosci 34:263-71. 2011
  9. ncbi request reprint Selective monoclonal antibody recognition and cellular localization of an unphosphorylated form of connexin43
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Exp Cell Res 236:127-36. 1997
  10. pmc Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 3J7, Canada
    J Comp Neurol 464:356-70. 2003

Collaborators

Detail Information

Publications39

  1. pmc Evidence for connexin36 localization at hippocampal mossy fiber terminals suggesting mixed chemical/electrical transmission by granule cells
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 745 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0J9
    Brain Res 1487:107-22. 2012
    ..This article is part of a Special Issue entitled Electrical Synapses...
  2. pmc Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Glia 44:205-18. 2003
    ..Reductions in Cx30 and Cx26 after Cx32 deletion suggest that these astrocytic connexins likely form junctions with Cx32 and that their incorporation into A/O gap junctions is dependent on the presence of oligodendrocytic Cx32...
  3. ncbi request reprint Connexin26 in adult rodent central nervous system: demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3J7
    J Comp Neurol 441:302-23. 2001
    ....
  4. pmc Astrocyte and oligodendrocyte connexins of the glial syncytium in relation to astrocyte anatomical domains and spatial buffering
    James I Nagy
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Cell Commun Adhes 10:401-6. 2003
    ....
  5. ncbi request reprint Update on connexins and gap junctions in neurons and glia in the mammalian nervous system
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue Winnipeg, Manitoba, Canada R3E 3J7
    Brain Res Brain Res Rev 47:191-215. 2004
    ....
  6. ncbi request reprint A brain slice model for in vitro analyses of astrocytic gap junction and connexin43 regulation: actions of ischemia, glutamate and elevated potassium
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Eur J Neurosci 12:4567-72. 2000
    ..These processes can be related in part to neuronal regulation of astrocytic gap junctional coupling state, which is also amenable to analysis in brain slices...
  7. ncbi request reprint Connexins and gap junctions of astrocytes and oligodendrocytes in the CNS
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Brain Res Brain Res Rev 32:29-44. 2000
    ....
  8. pmc Connexin26 expression in brain parenchymal cells demonstrated by targeted connexin ablation in transgenic mice
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, 745 Bannatyne Ave, Winnipeg, MB, Canada R3E 0J9
    Eur J Neurosci 34:263-71. 2011
    ....
  9. ncbi request reprint Selective monoclonal antibody recognition and cellular localization of an unphosphorylated form of connexin43
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Exp Cell Res 236:127-36. 1997
    ....
  10. pmc Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system
    James I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 3J7, Canada
    J Comp Neurol 464:356-70. 2003
    ....
  11. pmc Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain
    X Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Neuroscience 126:611-30. 2004
    ..Further, our findings suggest a scaffolding and/or regulatory role of ZO-1 at the oligodendrocyte side of astrocyte-to-oligodendrocyte gap junctions...
  12. pmc Association of connexin36 and zonula occludens-1 with zonula occludens-2 and the transcription factor zonula occludens-1-associated nucleic acid-binding protein at neuronal gap junctions in rodent retina
    C Ciolofan
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Neuroscience 140:433-51. 2006
    ....
  13. ncbi request reprint Identification of sequence, protein isoforms, and distribution of the hyaluronan-binding protein RHAMM in adult and developing rat brain
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3J7
    J Comp Neurol 439:315-30. 2001
    ..The widespread expression of RHAMM in adult and developing brain implies a role for this protein and its ligand hyaluronan in key events of cell signaling and cytoskeletal regulation in the CNS...
  14. pmc Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, Manitoba, Canada
    Eur J Neurosci 28:1503-17. 2008
    ....
  15. ncbi request reprint Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina
    C Ciolofan
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Neuroscience 148:473-88. 2007
    ....
  16. ncbi request reprint Connexin30 in rodent, cat and human brain: selective expression in gray matter astrocytes, co-localization with connexin43 at gap junctions and late developmental appearance
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Neuroscience 88:447-68. 1999
    ..Taken together, these results suggest that astrocytic connexin30 expression at both regional and cellular levels is subject to regulation in adult brain as well as during brain development...
  17. doi request reprint The effector and scaffolding proteins AF6 and MUPP1 interact with connexin36 and localize at gap junctions that form electrical synapses in rodent brain
    X Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, 745 Bannatyne Ave, Winnipeg, Manitoba, Canada
    Eur J Neurosci 35:166-81. 2012
    ....
  18. doi request reprint Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Eur J Neurosci 34:1783-93. 2011
    ..In addition, Cx30 protein may be required for co-stabilization of gap junctions or for co-trafficking in cells...
  19. pmc Connexin29 expression, immunocytochemistry and freeze-fracture replica immunogold labelling (FRIL) in sciatic nerve
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, R3E 3J7 Canada
    Eur J Neurosci 16:795-806. 2002
    ..In addition, Cx29 is present in distinctive IMP arrays in the inner most layer of myelin, adjacent to internodal axonal plasma membranes, where this connexin may have previously unrecognized functions...
  20. pmc Under construction: building the macromolecular superstructure and signaling components of an electrical synapse
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada
    J Membr Biol 245:303-17. 2012
    ....
  21. ncbi request reprint Subcellular distribution, calmodulin interaction, and mitochondrial association of the hyaluronan-binding protein RHAMM in rat brain
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    J Neurosci Res 65:6-16. 2001
    ....
  22. ncbi request reprint Elevated connexin43 immunoreactivity at sites of amyloid plaques in Alzheimer's disease
    J I Nagy
    Department of Physiology, University of Manitoba, Winnipeg, Canada
    Brain Res 717:173-8. 1996
    ....
  23. ncbi request reprint Enrichment of neuronal and glial connexins in the postsynaptic density subcellular fraction of rat brain
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, R3E 3J7, Manitoba, Canada
    Brain Res 898:1-8. 2001
    ..Thus, protocols used to isolate PSDs may serve as a basis for development of methods to isolate CNS gap junctions, which would aid biochemical identification of regulatory and structural proteins associated with these structures...
  24. ncbi request reprint Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells
    B D Lynn
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, R3E 3J7, Manitoba, Canada
    Neurosci Lett 306:49-52. 2001
    ..Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells...
  25. pmc Neuronal connexin36 association with zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO-1
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Ave, Winnipeg, Manitoba, Canada R3E 3J7
    Eur J Neurosci 19:2132-46. 2004
    ..These results demonstrating a Cx36/ZO-1 association suggest a regulatory and/or scaffolding role of ZO-1 at gap junctions that form electrical synapses between neurons in mammalian brain...
  26. ncbi request reprint Ultrastructural immunolocalization of adenosine deaminase in histaminergic neurons of the tuberomammillary nucleus of rat
    T Yamamoto
    Department of Physiology, University of Manitoba, Winnipeg, Canada
    Brain Res 527:335-41. 1990
    ..Immunolocalization of ADA therefore represents a reliable and convenient method for the identification of TM neurons in EM studies of their ultrastructure and synaptic interactions...
  27. ncbi request reprint Pharmacokinetics of 2'-deoxycoformycin, an inhibitor of adenosine deaminase, in the rat
    J D Geiger
    Department of Pharmacology, University of Manitoba, Faculty of Medicine, Winnipeg, Canada
    Neuropharmacology 26:1383-7. 1987
    ..The failure of the activity of ADA in brain to recover after treatment with 18.6 mumol/kg suggests either long-term down-regulation of the expression of ADA or irreversible damage to ADA-containing neurons...
  28. ncbi request reprint Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, R3E 3J7, Canada
    Histochem Cell Biol 122:485-98. 2004
    ....
  29. ncbi request reprint Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain
    Mihai C Penes
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Ave, Winnipeg, Manitoba R3E 3J7, Canada
    Eur J Neurosci 22:404-18. 2005
    ..Given previous observations that ZONAB and ZO-1 in combination regulate gene expression, our results suggest roles of glial gap junction-mediated anchoring of signalling molecules in a wide variety of glial homeostatic processes...
  30. doi request reprint Mouse Hyal3 encodes a 45- to 56-kDa glycoprotein whose overexpression increases hyaluronidase 1 activity in cultured cells
    Richard Hemming
    Department of Biochemistry and Medical Genetics, The University of Manitoba, Winnipeg, Canada
    Glycobiology 18:280-9. 2008
    ..The results indicate that Hyal3 overexpressed in cultured cells lacks intrinsic hyaluronidase activity and that Hyal3 may contribute to HA metabolism by augmenting the activity of Hyal1...
  31. ncbi request reprint Zebrafish Cx35: cloning and characterization of a gap junction gene highly expressed in the retina
    Elizabeth McLachlan
    Department of Zoology, University of Manitoba, Winnipeg, Canada
    J Neurosci Res 73:753-64. 2003
    ..A similar pattern was seen in the 4 and 5 dpf retina, but no labeling was detected in the retina of earlier embryos...
  32. ncbi request reprint Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins
    Vincent C Chen
    Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada
    J Proteome Res 5:2123-34. 2006
    ....
  33. pmc Direct association of connexin36 with zonula occludens-2 and zonula occludens-3
    Xinbo Li
    Department of Physiology, University of Manitoba, Winnipeg, Canada
    Neurochem Int 54:393-402. 2009
    ..These findings, together with the known association of ZO-2 with a variety of proteins, including transcription factors, suggest that ZO-2 may serve to anchor regulatory proteins at gap junctions composed of Cx36...
  34. pmc Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1
    Xinbo Li
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    J Neurosci 28:9769-89. 2008
    ..These data document that in Cx45-expressing neurons of IPL, Cx45 is almost always accompanied by Cx36, forming "bihomotypic" gap junctions, with Cx45 structurally coupling to Cx45 and Cx36 coupling to Cx36...
  35. ncbi request reprint Characterization of connexin30.3-deficient mice suggests a possible role of connexin30.3 in olfaction
    Qingyi Zheng-Fischhöfer
    Institut fur Genetik, Abteilung Molekulargenetik, Römerstrassae 164, D 53117 Bonn, Germany
    Eur J Cell Biol 86:683-700. 2007
    ..Furthermore, they showed normal auditory thresholds as measured by brain stem evoked potentials. These mice did, however, exhibit reduced behavioural responses to a vanilla scent...
  36. pmc Interaction between connexin35 and zonula occludens-1 and its potential role in the regulation of electrical synapses
    Carmen E Flores
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 105:12545-50. 2008
    ..The interaction of ZO-1 with conserved regions of the C termini of Cx35/Cx36 orthologs may have a common function at electrical synapses of mammals and other vertebrates...
  37. pmc Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb
    John E Rash
    Department of Biomedical Sciences, Colorado State University, Fort Collins, 80523, USA
    J Neurocytol 34:307-41. 2005
    ....
  38. ncbi request reprint Dynamics of electrical transmission at club endings on the Mauthner cells
    Alberto E Pereda
    Department of Neuroscience, Albert Einstein College of Medicine, New York, USA
    Brain Res Brain Res Rev 47:227-44. 2004
    ..The widespread distribution of connexin35 and connexin36 and the ubiquity of some of the proposed regulatory elements suggest that other electrical synapses may be similarly regulated...
  39. ncbi request reprint Characterization of connexin31.1-deficient mice reveals impaired placental development
    Qingyi Zheng-Fischhöfer
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, D 53117 Bonn, Germany
    Dev Biol 312:258-71. 2007
    ..We conclude that Connexin31.1 is critical for normal placental development but appears to be functionally compensated by other connexin isoforms in the embryo proper and adult mouse...